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Cross-linked Polyethylenimine as Potential DNA Vector for Gene Delivery with High Efficiency and Low Cytotoxicity

Acta Biochimica et Biophysica Sinica 2006年第11期38卷 780-787页

作者： Wei DONG~(1,2) Guang-Hui JIN~1 Shu-Feng LI~(1,3) Qi-Ming SUN~1 Ding-Yuan MA~1 Zi-Chun HUA~1 1 State Key Laboratory of Pharmaceutical Biotechnology,college of Life science,nanjing university,nanjing 210093,china 2 School of Chemical Engineering nanjing university of science and Technology,nanjing 210094,china 3 college of basic medical science,southeast university,nanjing 2.0009,china State Key Laboratory of Pharmaceutical Biotechnology College of Life Science Nanjing UniversityNanjing 210093 China School of Chemical Engineering Nanjing University of Science and TechnologyNanjing 210094 China college of basic medical science Southeast UniversityNanjing 210009 China

Polyethylenimine (PEI) has been known as an efficient gene carrier with the highest cationiccharge *** transfection efficiency of PEI,along with its cytotoxicity,strongly depends on itsmolecular *** enhance its gene delivery efficiency and minimize cytotoxicity,we have synthesizedsmall cross-linked PEI with biodegradable linkages and evaluated their transfection efficiencies in *** study,branched PEI with a molecular weight of 800 Da was cross-linked by small diacrylate[1,4-butanediol diacrylate or ethyleneglycol dimethacrylate (EGDMA)] for 2-6 *** efficiencies of thecross-linked PEI in in vitro transfection of plasmid DNA containing enhanced green fluorescent protein(EGFP) reporter gene were assessed in melanoma B 16F10 cell line and other cell *** cytometrywas used to quantify the cellular entry efficiency of plasmid and the transgene expression *** of the cross-linked PEI in these cells were evaluated by MTT ***-PEI 800-4h,atypical cross-linked PEI reported here,mediated a more efficient expression of reporter gene than thecommercially available 25-kDa branched PEI control,and resulted in a 9-fold increase in gene deliveryin B16F10 cells and a 16-fold increase in 293T cells,while no cytotoxicity was found at the optimizedcondition for gene ***,the transfection activity of polyplexes was preserved in thepresence of serum proteins.

关键词： polyethylenimine gene delivery cytotoxicity B16F10 293T cell line

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Dynamic damage and stress-strain relations of ultra-high performance cementitious composites subjected to repeated impact

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science china(Technological sciences) 2010年第6期53卷 1520-1525页

作者： LAI JianZhong1 & SUN Wei2 1 Department of Materials science and Engineering,nanjing university of science and Technology,nanjing 210094,china 2 college of Materials science and Engineering,southeast university,nanjing 211189,china Department of Materials science and Engineering Nanjing University of Science and Technology Nanjing China college of Materials science and Engineering Southeast University Nanjing China

Ultra-high performance cementitious composites (UHPCC) were prepared by replacing 60% of cement with ultra-fine industrial waste *** dynamic damage and compressive stress-strain relations of UHPCC were studied using split Hopkinson pressure bar (SHPB).The damage of UHPCC subjected to repeated impact was measured by the ultrasonic pulse velocity *** show that the dynamic damage of UHPCC increases linearly with impact times and the abilities of repeated impact resistance of UHPCC are improved with increasing fiber volume *** stress waves on impact were recorded and the average stress,strain and strain rate of UHPCC were calculated based on the wave propagation *** effects of strain rate,fibers volume fraction and impact times on the stress-strain relations of UHPCC were *** show that the peak stress and elastic modulus decrease while the strain rate and peak strain increase gradually with increasing impact times.

关键词： ultra-high performance cementitious composites (UHPCC) split Hopkinson pressure bar (SHPB) repeated impact damage stress-strain

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Spatial Chaos of Bose-Einstein Condensates in a Cigar-Shaped Trap

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Communications in Theoretical Physics 2007年第7期48卷 107-111页

作者： LI Fei REN Zhong-Zhou LUO Hai-Lu SHU Wei-Xing Department of Physics,nanjing university,nanjing 210008,chinaWU Qin School of basic medical science,Guangdong medical college,Dongguan 523808,china Department of Physics Nanjing University Nanjing 210008 China School of basic medical science Guangdong Medical College Dongguan 523808 China

The spatial chaos of Bose-Einstein condensates in a cigar-shaped trap is *** a system with asteady current,we construct the general solution of the 1st-order *** the boundedness condition of thegeneral solution, we obtain the Melnikov function predicting the onset of *** unpredictability of the system's dis-tribution of atom density is also theoretically *** a ^(23)Na system meeting the perturbation oondition,numericalsimulations show the existence of chaos,which is in accordance with our analytical *** simulations of a^(87)Rb system dissatisfying the perturbation condition also demonstrate that there exists chaos in the *** casewithout a current is also investigated.

关键词： Bose-Einstein condensates Melnikov function chaos

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basic study on architectural planning of psychiatric hospital

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science china(Technological sciences) 2010年第7期53卷 1755-1767页

作者： ZHOU Ying1 & SUN YaoNan2 1 southeast university, nanjing 210096, china 2 nanjing university of science & Technology, nanjing 210094, china southeast university Nanjing China nanjing university of science & Technology Nanjing China

This paper considers the main problems of the current mental health facilities in china from the standpoint of architects on the background that mental disorder has become a serious threat to human health. In order to effectively learn from the developed countries, this paper reviews the brief history of the world's mental health and the main phases of the evolution of psychiatric hospitals, analyzes the actual situation of mental health facilities abroad based on a large amount of data, compares the roles of public and private psychiatric hospitals from the viewpoint of government supervision, and investigates the functions and positions of psychiatric hospitals and the psychiatry divisions of general hospitals in the medical system. At last, this paper discusses the necessity of the systemization of mental health facilities, the role of psychiatric hospitals in this facility system and the developing approaches of psychiatric hospitals in china.

关键词： medical facility for mental disorder facility system psychiatric hospital principles

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Microfilament-binding properties of N-terminal extension of the isoform of smooth muscle long myosin light chain kinase

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Cell Research 2006年第4期16卷 367-376页

作者： Chun Xiang Yang~(1,3,*) Hua Qun Chen~(2,*) Chen Chen~1 Wei Ping Yu~3 Wen Cheng Zhang~1 Ya Jin Peng~1 Wei Qi He~1 Dong Mei Wei~1 Xiang Gao~1 Min Sheng Zhu~(1,4)~1Model Animal Research Center and National Key Lab of Medicine,nanjing university,nanjing 210061,china,~2college of Life science,nanjing Normal Unversity,nanjing 210097,china ~3medical college,southeast university,nanjing 210096,china ~4Huadong Research Institute for medical Biotechnics,Nanjiang 210002,china Model Animal Research Center and National Key Lab of Medicine Nanjing University Nanjing China medical college Southeast University Nanjing China college of Life science Nanjing Normal University Nanjing China Huadong Research Institute for medical Biotechnics Nanjing China

Myosin light chain kinases (MLCK) phosphorylate the regulatory light chain of myosin Ⅱ in thick filaments andbind to F-actin-containing thin filaments with high *** ability of short myosin light chain kinase (S-MLCK)to bind F-actin is structurally attributed to the DFRXXL regions in its *** long myosin light chain kinase(L-MLCK) has two additional DFRXXL motifs and six lg-like modules in its N-terminal *** six lg-likemodules are capable of binding to stress fibers *** results from the imaging analysis demonstrated thatthe first two intact lg-like modules (21g) in N-terminal extension of L-MLCK is the minimal binding module required formicrofilament *** assay confirmed that F-actin was able to bind *** of2lg peptide weresimilar for myofilament or pure *** binding affinities were slightly lower than 5DFRXXL peptide as *** to DFRXXL peptides,the 2Ig peptide also caused efficient F-actin bundle formation in *** theliving cell,over-expression of 2Ig fragment increased"spike"-like protrusion formation with over-bundled *** suggest that L-MLCK may act as a potent F-actin bundling protein via its DFRXXL region and the 2Ig region,implying that L-MLCK plays a role in cytoskeleton organization.

关键词： MLCK F-actin bundle Ig-like module

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Stable Skin-specific Overexpression of Human CTLA4-Ig in Transgenic Mice through Seven Generations

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Acta Biochimica et Biophysica Sinica 2006年第3期38卷 171-178页

作者： Yong WANG~1 Yong NI~(1,3) Hong WEI~1* Feng-Chao WANG~2 Liang-Peng GE~1 Xiang GAO~41 Department of Animal science,college of basic Medicine,Third Military medical university,Chongqing 400038,china 2 Institute of Combined Injury,college of Military Preventive Medicine,Third Military medical university,Chongqing 400038,china 3 School of Life sciences,Southwest university,Chongqing 400715,china 4 Animal Model Research Institute,nanjing university,nanjing 210093,china Department of Animal science College of Basic Medicine Third Military Medical UniversityChongqing 400038 China School of Life sciences Southwest UniversityChongqing 400715 China Institute of Combined Injury College of Military Preventive Medicine Third Military Medical UniversityChongqing 400038 China Animal Model Research Institute Nanjing UniversityNanjing 210093 China

Skin graft rejection is a typical cellular immune response,mainly mediated by T *** lymphocyte associated antigen 4-immunoglobin (CTLA4-Ig) extends graft survival by blocking the T cellco-stimulation pathway and inhibiting T cell *** investigate the efficacy of CTLA4-Ig in prolong-ing skin graft survival,human CTLA4-Ig (hCTLA4-Ig) was engineered to overexpress in mouse skin bytransgenesis using the K14 *** transcription-polymerase chain reaction (RT-PCR) and West-ern blot assay indicated that the expression of CTLA4-Ig remained skin-specific and relatively constantcompared to the internal control protein,AKT,through seven *** presence and concentrationof the hCTLA4-Ig protein in transgenic mouse sera was determined by enzyme-linked immunosorbent assay(ELISA),and the results indicated that the serum CTLA4-Ig concentration also remained constant *** of transgenic mouse skins grafted onto rat wounds was remarkably prolonged com-pared to that of wild-type skins from the same mouse strain,and remained comparable among all *** suggested that the bioactive hCTLA4-Ig protein was stably expressed in transgenical micethrough at least seven generations,which was consistent with the stable skin-specific CTLA4-Ig *** results demonstrated that the transgenic expression of hCTLA4-Ig in skin driven by the K14 promoterremained constant through generations,and a transgenic line can be established to provide transgenic skinwith extended survival reproducibly.

关键词： cytotoxic T lymphocyte antigen 4-Ig transgenic mice skin K14 promoter

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Dynamic Mechanical Behaviour of Ultra-high Performance Fiber Reinforced Concretes

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Journal of Wuhan university of Technology(Materials science) 2008年第6期23卷 938-945页

作者：赖建中 Department of Materials science & Engineering Nanjing University of Science & Technology

Ultra-high performance fiber reinforced concretes （UHPFRC） were prepared by replacing 60% of cement with ultra-fine industrial waste powder. The dynamic mechanical behaviour of UHPFRC with different fiber volume fraction was researched on repeated compressive impact in four kinds of impact modes through split Hopkinson pressure bar （SHPB）. The experimental results show that the peak stress and elastic modulus decrease and the strain rate and peak strain increase gradually with the increasing of impact times. The initial material damage increases and the peak stress of the specimen decreases from the second impact with the increasing of the initial incident wave. Standard strength on repeated impact is defined to compare the ability of resistance against repeated impact among different materials. The rate of reduction of standard strength is decreased by fiber reinforcement under repeated impact. The material damage is reduced and the ability of repeated impact resistance of UHPFRC is improved with the increasing of fiber volume fraction.

关键词： ultra-high performance fiber reinforced concretes split Hopkinson pressurebar dynamic repeated impact

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MMSE relay precoding schemes based on quantization error compensation for multi-relay system

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science china(Information sciences) 2010年第12期53卷 2548-2556页

作者： LI Ting 1,2 & YANG LuXi 2 1 college of Communication and Information Engineering,nanjing university of Posts and Telecommunications,nanjing 210003,china 2 School of Information science and Engineering,southeast university,nanjing 210096,china college of Communication and Information Engineering Nanjing University of Posts and Telecommunications Nanjing China School of Information science and Engineering Southeast University Nanjing China

In order to deal with the performance degradation resulting from the quantization error,this paper introduces the statistics of Grassmannian codebook's quantization error into relay precoding design for multirelay *** on the two-step MMSE optimization algorithm for relay precoding and receiver equalizing with full feedback under QoS requirements,we first propose a limited feedback two-step MMSE optimization scheme for relay precoding and receiver equalizing with quantization error compensation,and then we propose a limited feedback joint MMSE optimization scheme for relay precoding and receiver equalizing with quantization error compensation subject to a global power constraint at the output of *** results show that the proposed joint MMSE optimization scheme with quantization error compensation has a much lower bit-error rate than the two-step MMSE optimization scheme with quantization error compensation and the joint MMSE optimization scheme without quantization error compensation.

关键词： multi-relay precoding limited feedback minimum mean square error

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Anticancer effect of two diterpenoid compounds isolated from Annona glabra Linn

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Acta Pharmacologica Sinica 2004年第7期25卷 91-96页

作者： Yong-hong ZHANG2, Hai-yan PENG3, Guo-hao XIA3, Ming-yan WANG3, Ying HAN4 First Clinical college 3basic Medicine college, nanjing university of TraditionalChinese Medicine, nanjing 210029 4Jiangsu Institute of Materia Medica, nanjing 2.0009, china First Clinical college，nanjing university of Traditional Chinese Medicine Nanjing 210009 basic Medicine college Nanjing University of Traditional Chinese Medicine Nanjing 210029 Jiangsu Institute of Materia Medica Nanjing 210009

AIM: To study the inhibitory effect of two diterpenoid compounds isolated from Annona glabra Linn (Cunabic acid and ent-kauran-19-al-17-oic acid) on the proliferation of Human Liver Cancer (HLC) cell line SMMC-7721 and its mechanism. METHODS: Inhibition of cell proliferation was measured by MTT assay. The morphological changes of SMMC-7721 cells were observed under inverted phase-contrast microscope, fluorescent microscope, transmission electron microscope (TEM), and scanning electron microscope (SEM). Flow cytometer (FCM) was used to calculate the cell apoptotic rate, and immunohistochemical staining was used to observe the regulation of gene expression. RESULTS: The proliferation of SMMC-7721 cells was obviously inhibited after being treated with Cunabic acid at the concentration >5 μmol/L and ent-kauran-19-al-17-oic acid >10 μmol/L. The biggest inhibitory effect was 81.05 % when treated with Cunabic acid at the concentration of 25 μmol/L. The effect had a linear relationship with concentration. The result indicated that drug-treated cells exhibit typical morphological changes of apoptosis, including condensed chromatin and a reduction in volume. Sub-G0/G1 peak was found by FCM analysis and the cell cycle was arrested at G0/G1 stage. The apoptotic rates of the cells treated by Cunabic acid and ent-kauran-19-al-17-oic acid were 43.31 % and 24.95 %, respectively. It was visualized by immunohis- tochemical staining that the drugs down-regulated the gene expression of bcl-2 gene and up-regulated that of bax gene. CONCLUSION: The two diterpenoid compounds isolated from Annona glabra Linn, Cunabic acid and ent- kauran-19-al-17-oic acid can obviously inhibit the proliferation of HLC cell line SMMC-7721. The mechanism is correlated with the induction of cell apoptosis by down-regulating the gene expression of bcl-2 gene and up- regulating that of bax gene.

关键词： Annona glabra Linn diterpenes hepatocellular carcinoma apoptosis gene expression

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A NOVEL INTERPRETATION OF CONCENTRATION DEPENDENCE OF VISCOSITY OF DILUTE POLYMER SOLUTION

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Chinese Journal of Polymer science 2000年第1期18卷 57-67页

作者： Yan Pan Rong-shi Cheng college of Material science and Engineering, South china university of Technology, Guangzhou 510641, china college of Chemistry and Chemical Engineering, nanjing university, nanjing 210093, china 华南理工大学广东广州

The concentration dependence of the reduced viscosity of dilute polymer solution is interpreted in the light of a new concept of the self-association of polymer chains in dilute solution. The apparent self-association constant is defined as the molar association constant divided by the molar mass of individual polymer chain and is numerically interconvertible with the Huggins coefficient. The molar association constant is directly proportional to the effective hydrodynamic volume of the polymer chain in solution and is irrespective of the chain architecture. The effective hydrodynamic volume accounts for the non-spherical conformation of a short polymer chain in solution and is a product of a shape factor and hydrodynamic volume. The observed enhancement of Huggins coefficient for short chain and branched polymer is satisfactorily interpreted by the concept of self-association. The concept of self-association allows us to predict the existence of a boundary concentration C-s(dynamic contact concentration) which divides the dilute polymer solution into two regions.

关键词： self-association polymer solution viscosity concentration dependence

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