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Cross-linked Polyethylenimine as Potential DNA Vector for Gene Delivery with High Efficiency and Low Cytotoxicity

Acta Biochimica et Biophysica Sinica 2006年第11期38卷 780-787页

作者： Wei DONG~(1,2) Guang-Hui JIN~1 Shu-Feng LI~(1,3) Qi-Ming SUN~1 Ding-Yuan MA~1 Zi-Chun HUA~1 1 State Key Laboratory of Pharmaceutical Biotechnology,college of Life science,nanjing university,nanjing 210093,china 2 School of Chemical Engineering nanjing university of science and Technology,nanjing 210094,china 3 college of basic medical science,southeast university,nanjing 210009,china State Key Laboratory of Pharmaceutical Biotechnology College of Life Science Nanjing UniversityNanjing 210093 China School of Chemical Engineering Nanjing University of Science and TechnologyNanjing 210094 China college of basic medical science Southeast UniversityNanjing 210009 China

Polyethylenimine (PEI) has been known as an efficient gene carrier with the highest cationiccharge *** transfection efficiency of PEI,along with its cytotoxicity,strongly depends on itsmolecular *** enhance its gene delivery efficiency and minimize cytotoxicity,we have synthesizedsmall cross-linked PEI with biodegradable linkages and evaluated their transfection efficiencies in *** study,branched PEI with a molecular weight of 800 Da was cross-linked by small diacrylate[1,4-butanediol diacrylate or ethyleneglycol dimethacrylate (EGDMA)] for 2-6 *** efficiencies of thecross-linked PEI in in vitro transfection of plasmid DNA containing enhanced green fluorescent protein(EGFP) reporter gene were assessed in melanoma B 16F10 cell line and other cell *** cytometrywas used to quantify the cellular entry efficiency of plasmid and the transgene expression *** of the cross-linked PEI in these cells were evaluated by MTT ***-PEI 800-4h,atypical cross-linked PEI reported here,mediated a more efficient expression of reporter gene than thecommercially available 25-kDa branched PEI control,and resulted in a 9-fold increase in gene deliveryin B16F10 cells and a 16-fold increase in 293T cells,while no cytotoxicity was found at the optimizedcondition for gene ***,the transfection activity of polyplexes was preserved in thepresence of serum proteins.

关键词： polyethylenimine gene delivery cytotoxicity B16F10 293T cell line

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2023 Chinese national clinical practice guideline on diagnosis and management of Crohn’s disease

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中华医学杂志(英文版) 2024年第14期137卷 2024 Jul 2页

作者： Inflammatory Bowel Disease Group, Chinese Society of Gastroenterology, Chinese medical Association Inflammatory Bowel Disease Quality Control Center of china Xijing Hospital Air Force Medical University China Peking Union medical college Hospital China West china Hospital Sichuan University China The First Affiliated Hospital Dalian Medical University China university of Nottingham Ningbo China GRADE Centre United Kingdom The First Affiliated Hospital Sun Yat-sen University China Sir Run Run Shaw Hospital Zhejiang University School of Medicine China Peking university People's Hospital China The Second Affiliated Hospital Zhejiang University School of Medicine China Renmin Hospital Wuhan University China Chinese PLA General Hospital China The First Affiliated Hospital Naval Medical University China The Sixth Affiliated Hospital Sun Yat-sen University China The First Affiliated Hospital Anhui Medical University China Zhongshan Hospital Xiamen University China Zhongnan Hospital Wuhan University China The First Hospital of Shanxi medical university China The Eighth Affiliated Hospital Sun Yat-sen University China Peking university Third Hospital China Peking university First Hospital China Qilu Hospital Shandong University China General Hospital of Eastern Theater Command China Xiangya Hospital Central South University China The Tenth People's Hospital Tongji University China The First Affiliated Hospital Kunming Medical University China Shanghai Pudong New District Zhoupu Hospital China Ruijin Hospital Shanghai Jiao Tong University School of Medicine China Tongji medical college Huazhong University of Science and Technology China General Hospital of nanjing Military Command China The Third Xiangya Hospital Central South University China Daping Hospital Chongqing China The Second Xiangya Hospital Central South University China Jiangsu Provincial People's Hospital China The Second Hospital of Hebei medical university China nanjing Drum Tower Hospital China Nanfan Hospital Southern Medical University China GRADE Center University of Nottingham Ningbo China The Fifth Affiliated Hospital Zhengzhou University

The role of Chinese expert consensus in standardization and improvement of the clinical diagnosis and treatment of inflammatory bowel disease (IBD) is self-evident. As clinical and basic research in china becomes standardized under a better understanding of IBD, more data on Chinese patients have become available to develop a consensus and guideline. This guideline, collaboratively developed by the IBD Group, Chinese Society of Gastroenterology, incorporates the latest international consensus statements,[1-7] domestic research findings, and practical considerations, as an update based on the 2018 Chinese consensus on IBD management.[8] The IBD guideline consists of two parts: ulcerative colitis (UC) and Crohn's disease, and this manuscript presented the UC part. The evidence used in this guideline was collected and analyzed by the standard of guideline, and the contents were organized as problem statements, for clarity. The formulation of this guideline aimed to reflect the latest progress in IBD, providing comprehensive and valuable guidance for the clinical management of IBD.

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Effects of coffee and caffeine on bladder dysfunction in streptozotocininduced diabetic rats

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Acta Pharmacologica Sinica 2006年第8期27卷 1037-1043页

作者： Chao-ran YI~(2,5) Zhong-qing WEI~2 Xiang-lei DENG~4 Ze-yu SUN~2 Xing-rong LI~4 Cheng-gong TIAN~3 ~2Department of Urology,~3Department of Endocrinology,the Affiliated Drum Tower Hospital,nanjing university medical School,nanjing 210008,china ~4Department of Biochemisto,and Molecular Biology,Medieal college of southeast university,nanjing 210009,china Department of Urology the Affiliated Drum Tower Hospital Nanjing University Medical School Nanjing China Department of Biochemistry and Molecular Biology Medical College of Southeast University Nanjing China Department of Endocrinology the Affiliated Drum Tower Hospital Nanjing University Medical School Nanjing China

Aim:To explore the effects and mechanisms of caffeine and coffee on bladderdysfunction in streptozotocin-induced diabetic ***:Sprague-Dawleymale rats were divided randomly into 4 groups:control,diabetes mellitus(DM),DM with coffee treatment,and DM with caffeine *** diabetic rat wasinduced by intraperitoneal injection of streptozotocin(60 mg/kg).After 7 weeks oftreatment with coffee and caffeine,cystometrogram,contractile responses to elec-trical field stimulation(EFS)and acetylcholine(ACh),and cyclic AMP(cAMP)concentration of the bladder body and base were ***:The bladderweight,volume threshold for micturition and post-void residual volume(PVR)inthe diabetic rats were significantly higher compared to those in the control *** or caffeine treatment significantly reduced the bladder weight,bladdercapacity and PVR in the diabetic *** caused significant decreases in cAMPconcentration of the bladder and coffee and caffeine caused upregulation of cAMPcontent in the diabetic *** addition,coffee and caffeine tended to normal-ize the altered detrusor contractile responses to EFS and ACh in the diabetic ***:These results indicate that caffeine and coffee may have beneficialeffects on bladder dysfunction in the early stage of diabetes by increasing cAMPcontent in the lower urinary tract,recovering the micturition reflex and improvingthe detrusor contractility.

关键词： caffeine diabetic mellitus bladder urodynamics muscle contraction cyclic AMP

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Dynamic damage and stress-strain relations of ultra-high performance cementitious composites subjected to repeated impact

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science china(Technological sciences) 2010年第6期53卷 1520-1525页

作者： LAI JianZhong1 & SUN Wei2 1 Department of Materials science and Engineering,nanjing university of science and Technology,nanjing 210094,china 2 college of Materials science and Engineering,southeast university,nanjing 211189,china Department of Materials science and Engineering Nanjing University of Science and Technology Nanjing China college of Materials science and Engineering Southeast University Nanjing China

Ultra-high performance cementitious composites (UHPCC) were prepared by replacing 60% of cement with ultra-fine industrial waste *** dynamic damage and compressive stress-strain relations of UHPCC were studied using split Hopkinson pressure bar (SHPB).The damage of UHPCC subjected to repeated impact was measured by the ultrasonic pulse velocity *** show that the dynamic damage of UHPCC increases linearly with impact times and the abilities of repeated impact resistance of UHPCC are improved with increasing fiber volume *** stress waves on impact were recorded and the average stress,strain and strain rate of UHPCC were calculated based on the wave propagation *** effects of strain rate,fibers volume fraction and impact times on the stress-strain relations of UHPCC were *** show that the peak stress and elastic modulus decrease while the strain rate and peak strain increase gradually with increasing impact times.

关键词： ultra-high performance cementitious composites (UHPCC) split Hopkinson pressure bar (SHPB) repeated impact damage stress-strain

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Anticancer effect of two diterpenoid compounds isolated from Annona glabra Linn

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Acta Pharmacologica Sinica 2004年第7期25卷 91-96页

作者： Yong-hong ZHANG2, Hai-yan PENG3, Guo-hao XIA3, Ming-yan WANG3, Ying HAN4 First Clinical college 3basic Medicine college, nanjing university of TraditionalChinese Medicine, nanjing 210029 4Jiangsu Institute of Materia Medica, nanjing 210009, china First Clinical college，nanjing university of Traditional Chinese Medicine Nanjing 210009 basic Medicine college Nanjing University of Traditional Chinese Medicine Nanjing 210029 Jiangsu Institute of Materia Medica Nanjing 210009

AIM: To study the inhibitory effect of two diterpenoid compounds isolated from Annona glabra Linn (Cunabic acid and ent-kauran-19-al-17-oic acid) on the proliferation of Human Liver Cancer (HLC) cell line SMMC-7721 and its mechanism. METHODS: Inhibition of cell proliferation was measured by MTT assay. The morphological changes of SMMC-7721 cells were observed under inverted phase-contrast microscope, fluorescent microscope, transmission electron microscope (TEM), and scanning electron microscope (SEM). Flow cytometer (FCM) was used to calculate the cell apoptotic rate, and immunohistochemical staining was used to observe the regulation of gene expression. RESULTS: The proliferation of SMMC-7721 cells was obviously inhibited after being treated with Cunabic acid at the concentration >5 μmol/L and ent-kauran-19-al-17-oic acid >10 μmol/L. The biggest inhibitory effect was 81.05 % when treated with Cunabic acid at the concentration of 25 μmol/L. The effect had a linear relationship with concentration. The result indicated that drug-treated cells exhibit typical morphological changes of apoptosis, including condensed chromatin and a reduction in volume. Sub-G0/G1 peak was found by FCM analysis and the cell cycle was arrested at G0/G1 stage. The apoptotic rates of the cells treated by Cunabic acid and ent-kauran-19-al-17-oic acid were 43.31 % and 24.95 %, respectively. It was visualized by immunohis- tochemical staining that the drugs down-regulated the gene expression of bcl-2 gene and up-regulated that of bax gene. CONCLUSION: The two diterpenoid compounds isolated from Annona glabra Linn, Cunabic acid and ent- kauran-19-al-17-oic acid can obviously inhibit the proliferation of HLC cell line SMMC-7721. The mechanism is correlated with the induction of cell apoptosis by down-regulating the gene expression of bcl-2 gene and up- regulating that of bax gene.

关键词： Annona glabra Linn diterpenes hepatocellular carcinoma apoptosis gene expression

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Expert Consensus on the Prevention and Treatment of Chronic Hepatitis B in Children

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Infectious Diseases & Immunity 2024年第3期4卷 106-120页

作者： Chinese Society of Infectious Diseases Chinese medical Association Chinese Society of Hepatology Chinese medical Association Group of Infectious Diseases Chinese Pediatric Society Chinese medical Association National Clinical Research Center for Infectious Diseases(Beijing) Fu-Sheng Wang Shishu Zhu Min Zhang Jianshe Wang Shuangjie Li Junliang Fu Yuanyuan Li Yuming Guo Jing Li Hongmei Xu Wenxian Ouyang Yuemin Nan Sainan Shu Shuangsuo Dang Xiaoyuan Xu Taisheng Li Jinlin Hou 不详 The Fifth medical Center of PLA General Hospital Beijing 100039China Fudan university Children’s Hospital Hunan Children’s Hospital Children’s Hospital of Chongqing medical university Third Hospital Hebei Medical University Tongji Hospital Tongji Medical CollegeHuazhong University of Science and Technology The Second Affiliated Hospital of Xi’an Jiaotong university Peking university First Hospital Peking Union medical college Hospital Chinese Academy of Medical Sciences&Peking Union Medical College Nanfang Hospital Southern Medical University

The aim of this consensus is to standardize the prevention, diagnosis, and treatment of chronic hepatitis B in children and to achieve the goal of “eliminating viral hepatitis as a major public health threat by 2030” issued by the World Health Organization. Formulated by organized experts of the Chinese Society of Infectious Diseases and Chinese Society of Hepatology, Chinese medical Association;Group of Infectious Diseases, Chinese Pediatric Society, Chinese medical Association;and the National Clinical Research Center for Infectious Diseases (Beijing), the consensus provides the latest evidence and recommendations for the prevention, diagnosis, and treatment of chronic hepatitis B in children.

关键词： Chronic hepatitis B Children Treatment Prevention Consensus

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Statistical study of cement additives with and without chloride on performance modification of Portland cement

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Progress in Natural science:Materials International 2011年第3期21卷 246-253页

作者： Hong HUANG,Xiao-dong SHEN State Key Laboratory of Materials-Oriented Chemical Engineering,college of Material science and Engineering, nanjing university of Technology,nanjing 210009,china State Key Laboratory of Materials-Oriented Chemical Engineering College of Material Science and Engineering Nanjing University of Technology Nanjing 210009 China

The effectiveness of cement additives with and without chloride on the fluidity and the strength development of Portland cement was compared by using statistical full factorial *** experimental results show that the cement additive containing CaCl2and Ca(NO3)2can enhance early strength of cement ***,Ca(NO3)2is less effective than CaCl2even if it is combined with other organic chemicals such as alkanolamine and *** significant difference is found between CaCl2and Ca(NO3)2influencing the fluidity and 28 d strength of *** fluidity is determined by saccharide and its interaction with *** 28 d strength enhancement is determined by *** fluidity as well as the mechanical performance of treated cement can be significantly improved by combining Ca(NO3)2with TIPA and saccharide.

关键词： Portland cement cement additive accelerator type fluidity strength development full factorial design

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Spatial Chaos of Bose-Einstein Condensates in a Cigar-Shaped Trap

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Communications in Theoretical Physics 2007年第7期48卷 107-111页

作者： LI Fei REN Zhong-Zhou LUO Hai-Lu SHU Wei-Xing Department of Physics,nanjing university,nanjing 210008,chinaWU Qin School of basic medical science,Guangdong medical college,Dongguan 523808,china Department of Physics Nanjing University Nanjing 210008 China School of basic medical science Guangdong Medical College Dongguan 523808 China

The spatial chaos of Bose-Einstein condensates in a cigar-shaped trap is *** a system with asteady current,we construct the general solution of the 1st-order *** the boundedness condition of thegeneral solution, we obtain the Melnikov function predicting the onset of *** unpredictability of the system's dis-tribution of atom density is also theoretically *** a ^(23)Na system meeting the perturbation oondition,numericalsimulations show the existence of chaos,which is in accordance with our analytical *** simulations of a^(87)Rb system dissatisfying the perturbation condition also demonstrate that there exists chaos in the *** casewithout a current is also investigated.

关键词： Bose-Einstein condensates Melnikov function chaos

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Analysis of variance for testing method of cement in determination of strength

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Progress in Natural science:Materials International 2011年第4期21卷 341-346页

The statistical tools such as descriptive statistics,full factorial design and analysis of source of variation were used to identify the potential factors that impact the validity of testing method for determining the strength of *** results showed that personal error impacted both accuracy and precision of test *** time associated with temperature fluctuation resulted in strength variation but did not impact the precision of test in all curing *** compactions did not impact the precision of test but resulted in the strength variation on 3 d and 28 d *** methods for the initial moist air curing significantly impacted the precision of testing method and resulted in the strength variation of cement on 1 d.

关键词： testing method of cement analysis of variance strength accuracy precision

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传染病多点触发智慧化监测预警系统关键技术专家共识

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中华医学杂志 2024年第32期104卷 1-15页

作者：中国医学科学院北京协和医学院群医学及公共卫生学院北京大学公共卫生学院中国疾病预防控制中心传染病管理处四川大学华西公共卫生学院中国科学院地理科学与资源研究所首都医科大学附属北京地坛医院中华医学会公共卫生分会

有效的传染病监测预警系统是公共卫生安全体系的重要组成部分, 对预防与控制传染病暴发流行至关重要, 提升监测预警能力是推动疾病预防控制事业高质量发展的当务之急。专家组结合公共卫生与预防医学、临床医学、管理学、数据科学、计算... 详细信息

有效的传染病监测预警系统是公共卫生安全体系的重要组成部分, 对预防与控制传染病暴发流行至关重要, 提升监测预警能力是推动疾病预防控制事业高质量发展的当务之急。专家组结合公共卫生与预防医学、临床医学、管理学、数据科学、计算机科学等多学科专家的研究成果与实践经验, 经过反复多轮专家研讨, 形成了传染病多点触发智慧化监测预警系统关键技术专家共识。本共识内容主要包括传染病多点触发智慧化监测预警系统的相关概念和定义, 关键技术框架, 多渠道监测预警数据来源、获取与治理, 预警方法分类, 多点触发智慧化监测预警关键流程及路径, 多点触发预警及综合研判, 预警信号响应以及预警效果评价等方面, 以期为传染病多点触发智慧化监测预警系统建设及应用提供技术参考。

关键词：传染病监测预警多点触发智慧化

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