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Purification and Characterization of Two Endo-β-1,4-glucanases from Mollusca,Ampullaria crossean

Acta Biochimica et Biophysica Sinica 2005年第10期37卷 702-708页

作者： Yan-Hong LI~(1#) Rui GUO~(3#) Qiu-Yu YIN~1 Ming DING~1 Si-Liang ZHANG~3 Gen-Jun XU~(1,2) Fu-Kun ZHAO~(1,2*) 1 Key Laboratory of Proteomics,institute of Biochemistry and Cell Biology,shanghai institutes for Biological sciences.Graduate School of the Chinese Academy of sciences,Chinese Academy of sciences,shanghai 200031,china 2 College of Life science,Zhejiang Sci-Tech university,Hangzhou 310018,china 3 bioengineering institute,east china university of science and technology,shanghai 200233,china Key Laboratory of Proteomics Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Graduate School of the Chinese Academy of Sciences Chinese Academy of SciencesShanghai 200031 China bioengineering institute East China University of Science and TechnologyShanghai 200233 China College of Life science Zhejiang Sci-Tech UniversityHangzhou 310018 China

Two novel endo-β-1,4-glucanases,EG45 and EG27,were isolated from the gastric juice ofmollusca,Ampullaria crossean,by anion exchange,hydrophobic interaction,gel filtration and a secondround of anion exchange *** purified proteins EG45 and EG27 appeared as a single bandon sodium dodecylsulfate polyacrylamide gel electrophoresis with a molecular mass of 45 kDa and 27 kDa,*** optimum pH for CMC activity was 5.5 for EG45 and 4.4-4.8 for *** optimumtemperature range for EG27 was broad,between 50℃ and 60℃;for EG45 it was 50℃.The analysis on thestability of these two endo-β-1,4-glucanases showed that EG27 was acceptably stable at pH 3.0-11.0 evenwhen the incubation time was prolonged to 24 h at 30℃,whereas EG45 remained relatively stable at *** 85% of the activity of EG27 could be retained upon incubation at 60℃ for 24 ***,less than 10% residual activity of EG45 was detected at 50℃.Among different kinds of substrates,bothenzymes showed a high preference for carboxymethyl ***45,in particular,showed a carboxymethylcellulose hydrolytic activity of 146.5 IU/mg *** enzymes showed low activities to xylan (from oatspelt) and Sigmacell 101,and they were inactive to p-nitrophenyl-β-D-cellobioside,salicin and starch.

关键词： cellulase endo-β-1,4-glucanase Ampullaria crossean purification substrate specificity

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An improved approach to the efficient construction of and search operations in motion graphs

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science china(Information sciences) 2012年第5期55卷 1042-1051页

作者： GAO Yan 1,CHEN ZhiHua 2,CHEN MinGang 3 & SHEN Yang 3 1 Software Engineering institute,east china Normal university,shanghai 200062,china 2 Department of Computer science and Engineering,east china university of science and technology,shanghai 200237,china 3 Department of Computer science and Engineering,shanghai Jiao Tong university,shanghai 200240,china Software Engineering institute East China Normal University Shanghai China Department of Computer science and Engineering East China University of Science and Technology Shanghai China Department of Computer science and Engineering Shanghai Jiao Tong University Shanghai China

Motion graphs have been widely used as an effective technique in the synthesis of human *** synthesize well-formed motions,motion graphs require smooth transitions and good ***,the time and costs grow quadratically as the graph size *** this paper,we present a method to set up a hierarchical structure,called a structure *** coarse level of this graph models different motion categories and their transitions,and the fine level mainly captures different motion *** interpolation space method is used to improve the graph connectivity at the fine *** structure guides the establishment of a faster and more realistic graphical *** the building process,this structure also helps with searching the motion graph,by performing a traverse search and placing the findings into a direct look-up table or local search ***,when there is no direct link between the different motions,a navigation map is introduced to conduct an appropriate search path via a series of transition motion *** experimental results indicate that our approach could lower the computation time in construction and searching,while simultaneously providing a richer variety of motion styles.

关键词： motion graph structure table registration curve motion interpolation

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cDNA Cloning of Two Novel T-superfamily Conotoxins from Conus leopardus

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 287-291页

作者： Wei-Hua CHEN~(1,2#) Yu-Hong HAN~(2,3#) Qi WANG~(2,3#) Xiao-Wei MIAO~(2,4) Ling OU~1 Xiao-Xia SHAO~2* 1 bioengineering institute,east china university of science and technology,shanghai 200237,china 2 institute of Protein Research,Tongji university,shanghai 200092,china 3 institute of Biochemistry and Cell Biology,shanghai institute of Biochemical sciences,Chinese Academy of sciences,shanghai 200031,china 4 Department of Biological Engineering,School of Life science,shanghai university,shanghai 200436,china bioengineering institute East China University of Science and TechnologyShanghai 200237 China institute of Protein Research Tongji UniversityShanghai 200092 China institute of Biochemistry and Cell Biology Shanghai Institute of Biochemical Sciences Chinese Academy of SciencesShanghai 200031 China Department of Biological Engineering School of Life Science Shanghai UniversityShanghai 200436 China

The full-length cDNAs of two novel T-superfamily conotoxins,Lp5.1 and Lp5.2,were clonedfrom a vermivorous cone snail Conus leopardus using 3'/5'-rapid amplification of cDNA *** cDNA ofLp5.1 encodes a precursor of 65 residues,including a 22-residue signal peptide,a 28-residue propeptide anda 15-residue mature ***5.1 is processed at the common signal site -X-Arg- immediately before themature peptide *** the case of Lp5.2,the precursor includes a 25-residue signal peptide anda 43-residue sequence comprising the propeptide and mature peptide,which is probably cleaved to yield a29-residue propeptide and a 14-residue mature *** these two conotoxins share a similar signalsequence and a conserved disulfide pattern with the known T-superfamily,the pro-region and mature peptidesare of low identity,especially Lp5.2 with an identity as low as 10.7% compared with the reference *** elucidated cDNAs of these two toxins will facilitate a better understanding of the species distribution,the sequence diversity of T-superfamily conotoxins,the special gene structure and the evolution of thesepeptides.

关键词： Conus leopardus T-superfamily conotoxin cDNA cloning

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EMMA:An Efficient Massive Mapping Algorithm Using Improved Approximate Mapping Filtering

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 857-864页

作者： Xin ZHANG~1 Zhi-Wei CAO~2 Zhi-Xin LIN~3 Qing-Kang WANG~1* Yi-Xue LI~4 1 institute of Micro/Nano science and technology,shanghai Jiaotong university,shanghai 200030,china 2 shanghai Center for Bioinformation technology,shanghai 200235,china 3 College of Life science and technology,shanghai Jiaotong university,shanghai 200030,china 4 Bioinformation Center of shanghai institutes for Biological sciences,Chinese Academy of sciences,shanghai 200031,china institute of Micro/Nano science and technology Shanghai Jiaotong UniversityShanghai 200030 China shanghai Center for Bioinformation technology Shanghai 200235 China College of Life science and technology Shanghai Jiaotong UniversityShanghai 200030 China Bioinformation Center of shanghai institutes for Biological sciences Chinese Academy of SciencesShanghai 200031 China

Efficient massive mapping algorithm (EMMA),an algorithm on efficiently mapping massivecDNAs onto genomic sequences,has recently been *** process of mapping massive cDNAsonto genomic sequences has been improved using more approximate mapping filtering based on an enhancedsuffix array coupled with a pruned fast hash table,algorithms of block alignment extensions,and *** compared with the classical BLAT software in this field,the computing of EMMA ranges fromtwo to forty-one times faster under similar prediction precisions.

关键词： cDNA mapping maximal exact match enhanced suffix array pruned fast hash table extension algorithm

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Lie bialgebras of generalized Witt type

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science china Mathematics 2006年第4期49卷 533-544页

作者： SONG Guang’ai & SU Yucai College of Mathematics and Information science, Shandong institute of Business and technology, Yantai 264005, china Department of Mathematics, university of science and technology of china, Hefei 230026, china Department of Mathematics, shanghai Jiaotong university, shanghai 200240, china College of Mathematics and Information science Shandong Institute of Business and Technology Yantai China Department of Mathematics Shanghai Jiaotong University Shanghai China Department of Mathematics University of Science and Technology of China Hefei China

In this paper, all Lie bialgebra structures on the Lie algebras of generalized Witt type are considered. It is proved that, for any Lie algebra W of generalized Witt type, all Lie bialgebras on W are the coboundary triangular Lie bialgebras. As a by-product, it is also proved that the first cohomology group H1(W, W(?)W) is trivial.

关键词： Lie bialgebras, Yang-Baxter equation, Lie algebra of generalized Witt type.

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Density functional theory study on the structural and electronic properties of Ag-adsorbed (MgO)_n clusters

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science china(Physics,Mechanics & Astronomy) 2009年第5期52卷 734-741页

作者： GE GuiXian1, JING Qun1 & LUO YouHua2,3 1 Key Laboratory of Ecophysics and Department of Physics, Normal College, Shihezi university, Shihezi 832003, china 2 School of science, east china university of science and technology, shanghai 200237, china 3 institute for Theoretical Physics, Henan university, Kaifeng 475004, china Key Laboratory of Ecophysics and Department of Physics Normal College Shihezi University Shihezi China School of science East China University of Science and Technology Shanghai China institute for Theoretical Physics Henan University Kaifeng China

Equilibrium geometries, charge distributions, stabilities and electronic properties of the Ag-adsorbed (MgO)n (n = 1-8) clusters have been investigated by density functional theory (DFT) with generalized gradient approximation (GGA) for exchange-correlation functional. The results show that hollow site is energetically preferred for n≥4, and the incoming Ag atoms tend to cluster on the existing Ag cluster. The Mulliken populations indicate that the interaction between the Ag atom and Magnesia clusters is mainly induced by a weak atomic polarization. The adsorbed Ag atom only causes charge redistributions of the atoms near itself. The effect of the adsorbed Ag atom on the bonding natures and structural features of Magnesia clusters is minor. Furthermore, the investigations on the first energy difference, fragmentation energies and electron affinities show that the Ag(MgO)4 and Ag(MgO)6 are the most stable among studied clusters.

关键词： Ag (MgO)_n clusters geometries electronic properties

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A SEMI-CIRCLE THEOREM FOR UNSTABLE PERTURBATION IN TROPICAL CYCLONE-SCALE VORTICLES

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Journal of Hydrodynamics 2009年第4期21卷 473-481页

作者： HUANG Hong ZHANG Ming XU Ming institute of Meteorology PLA University of Science and Technology Nanjing 211101 China shanghai Typhoon institute China Meteorological Administration Shanghai 200030 China Laboratory of Typhoon Forecast Technique/CMA Shanghai 200030 China

In this paper, we discusses the spectrum distribution of baroclinic disturbances in tropical cyclone-scale vortices with the assumption that the basic flow does not vary with height. The semi-circle theorem for the unstable perturbation is obtained and the upper bound of growth rate can be estimated. The upper bound of instability growth rate increases with the increase of the maximum basic-state tangential wind speed, angular speed, inertial parameter, absolute vorticity, radial gradient of absolute vorticity and static stability. The upper bound of instability growth rate is greater for vortices of larger horizontal scale and smaller vertical scale and lower wave-number disturbances.

关键词： tropical cyclone-scale vortex spiral bands instability semi-circle theorem

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Identification of human dopamine D1-like receptor agonist using a cellbased functional assay

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Acta Pharmacologica Sinica 2005年第10期26卷 1181-1186页

作者： Nan JIANG~2, Ke-qing OU-YANG~3, Shao-xi CAI~3, Ying-he HU~2, Zhi-liang XU~(2,4) ~2 shanghai institute of Brain Functional Genomics and Key Lab of Brain Functional Genomics, Ministry of Education, science and technology Commission of shanghai Municipality, east china Normal Uuiversitv, shanghai 200062 ~3 College of bioengineering, Chongqing university, Chongqing 400044, china shanghai institute of Brain Functional Genomics and Key Lab of Brain Functional Genomics Ministry of Education Science and Technology Commission of Shanghai Municipality East China Normal University Shanghai China College of bioengineering Chongqing University Chongqing China

Aim: To establish a cell-based assay to screen human dopamine D1 and D5 receptor agonists against compounds from a natural product compound library. Methods: Synthetic responsive elements 6xcAMP response elements （CRE） and a mini promoter containing a TATA box were inserted into the pGL3 basic vector to generate the reporter gene construct pCRE/TA/Luci. CHO cells were co-trans- fected with the reporter gene construct and human D1 or D5 receptor cDNA in mammalian expression vectors. Stable cell lines were established for agonist screening. A natural product compound library from over 300 herbs has been established. The extracts from these herbs were used for human D1 and D5 receptor agonist screenings. Results: A number of extracts were identified thatactivated both D1 and D5 receptors. One of the herb extracts, SBG492, demonstrated distinct pharmacological characteristics with human D1 and D5 receptors. The EC50values of SBG492 were 342.7 μg/mL for the D1 receptor and 31.7 μg/mL for the D5 receptor. Conclusion: We have established a cell-based assay for high-throughput drug screening to identify D1-like receptor agonists from natural products. Several extracts that can active D1-like receptors were discovered. These compounds could be useful tools for studies onthe functions of these receptors in the brain and could potentially be developed into therapeutic drugs for the treatment of central nervous system diseases.

关键词： dopamine DI receptors drug screening Chinese herbal drugs

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P-twisted affine Lie algebra and its realizations by twisted vertex operators

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science china Mathematics 2005年第Z1期48卷 295-305页

作者： LU Keping, CHU Yanjun, ZHENG Zhujun & DONG Wenfeng institute of Mathematics, Henan university, Kaifeng 475001, china USTC shanghai institute for Advanced Studies, university of science and technology of china, shanghai 201315, china institute of Mathematics Henan University Kaifeng China USTC shanghai institute for Advanced Studies University of Science and Technology of China Shanghai China

In this paper, we define a P-twisted affine Lie algebra, and construct its realizations by twisted vertex operators.

关键词： P-twisted affine Lie algebra, twisted vertex operator, extended P-twisted afline algebra.

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Enhanced activity of yqhD oxidoreductase in synthesis of 1,3-propanediol by error-prone PCR

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Progress in Natural science:Materials International 2008年第12期18卷 1519-1524页

作者： Hongmei Li~a Jia Chen~a Yinghua Li~b a institute of Food science and Biological technology,university of shanghai for science and technology,shanghai 200093,china b institute of Pharmaceutical Engineering,Zhejiang university,Hangzhou 310027,china institute of Food science and Biological technology University of Shanghai for Science and Technology Shanghai 200093 China institute of Pharmaceutical Engineering Zhejiang University Hangzhou 310027 China

yqhD oxidoreductase was determined to be an NADP-dependent dehydrogenase,and was more active toward 3-HPA when compared to 1,3-propanediol *** further improve enzyme activity towards 3-hydroxypropionaldehyde (3-HPA),error-prone PCR was implemented to mutant yqhD *** mutants,D99QN147H and Q202A with increased catalytic and affinity efficiency,were obtained after one round of error-prone polymerase chain *** the catalytic efficiency of the mutant D99QN147H was up to 4-fold greater than the wild enzyme (0.0375 min-1mM-1vs.0.0078 min-1mM-1).The recombined strain containing pEY28yqhD D99QN147H yielded 28 g L-1of 1,3-propanediol in the fed-batch LB cultures (1 L volume) with an initial 3-HPA concentration of 40 g L-1,which was higher than the 21 and 17 g L-1of 1,3-propanediol from the mutant Q202A and the wild-type,*** for propionaldehyde,the optimal mutant D99QN147H also exhibited higher activity on a range of substituted aldehydes than the wild- type.

关键词： yqhD oxidoreductase Error-prone PCR Catalytic efficiency 1,3-Propanediol

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