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A preliminary molecular historical biogeography of Caragana (Leguminosae) based on ITS and trnL-F DNA sequence data

Journal of Arid Land 2009年第1期1卷 64-73页

作者： MingLi ZHANG1,2, Yun KANG3 , JunBo YANG4 1 Laboratory of Evolution and Biodiversity Conservation in Arid Region, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, china 2 Institute of Botany, Chinese Academy of Sciences, Beijing 100093, china 3 school of pharmacy, fudan university, shanghai 200032, china 4 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, china Chinese Acad Sci Lab Evolut & Biodivers Conservat Arid Reg Xinjiang Inst Ecol & Geog Urumqi 830011 Peoples R China Chinese Acad Sci Inst Bot Beijing 100093 Peoples R China fudan Univ Sch Pharm Shanghai 200032 Peoples R China Chinese Acad Sci Kunming Inst Bot Kunming 650204 Peoples R China

The nrDNA internal transcribed spacer (ITS) and cpDNA trnL-F internal spacer (IGS) sequence data of Caragana eight species and one outgroup Halimodendron halodendron, was employed to reconstruct a phylogenetic tree, then the area relationship was analyzed by means of component analysis (CA), Brooks parsimony analysis (BPA), and dispersal-vicariance analysis (DIVA), six areas were selected from two divided distributions of East Asia and Tethys in Caragana. The phylogenetic tree indicated that there were three distinctive groups, which were attributed to some morphological characters, first with pinnate foliage and deciduous rachis, second with palmate foliage and persistent sclerotic stick rachis, and third with pinnate foliage and persistent sclerotic stick rachis. The results of CA and BPA illustrated general area relationships. An explicit area relationship should be Altai-Sayan, Far East NE china and North china (Hengduan Mountains). DIVA recognized several explanatory vicariance and dispersal events. As the scenario of Caragana distribution pattern, it looks like the vicariance versus dispersal plays more important role. In vicaraince, there are not only the isolated far-distance vicariance, but also the adjacent vicariance especially a vicariance between Hengduan Mountains and North china.

关键词： Caragana ITS trnL-F phylogeny cladistic biogeography DIVA distribution pattern

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Conformational re-analysis of(+)-meptazinol:an opioid with mixed anal-gesic pharmacophores

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Acta Pharmacologica Sinica 2006年第9期27卷 1247-1252页

作者： Wei LI~2 Xing-hai WANG~2 Choi-wan LAU~3 Yun TANG~(2,4)Qiong XIE~2 Zhui-bai QIU~(2,5)2 Department of Medicinal Chemistry 3 NMR Laboratory,school of pharmacy,fudan university,shanghai 200032,china 4 school of pharmacy,East china university of Science and Technology,shanghai 200237,china DepartmentofMedicinalChemistry NMRLaboratory SchoolofPharmacyFudanUniversityShanghai200032China schoolofpharmacy EastChinaUniversityofScienceandTechnologyShanghai200237China

Aim:To further investigate the analgesic pharmacophore of(+)-***:Two different opioid pharmacophores,Pharm-Ⅰ and Pharm-Ⅱ,were es-tablished from structures of nine typical opiates and meperidine by using molecu-lar modeling approaches according to their different structure activity relation-ship *** were further validated by a set of conformationally con-strained *** conformers of(+)-meptazinol(Conformer-Ⅰ and Con-former-Ⅱ)detected in solution were then fitted into the pharmacophores,respectively,by Fit Atoms facilities available in SYBYL,a computational modelingtool kit for molecular design and ***:Conformer-Ⅰ fit Pharm-Ⅰ fromtypical opiates ***,Conformer-Ⅱ fit none of these ***,it was found to be similar to another potent analgesic,benzofuro[2,3-c]pyridin-6-ol,whose pharmacophore was suggested to hold the transitional statebetween the two established *** typical analgesics derivedfrom 4-aryl piperidine(eg,meperidine)with one conformer absolutely overwhelming,the(+)-meptazinol exists in two conformers with similar amounts in ***,both conformers can not transform to each other freely in ordinaryconditions based on our NMR ***:(+)-meptazinol was suggestedto be an opioid with mixed analgesic pharmacophores,which may account for thecomplicated pharmacological properties of meptazinol.

关键词： (+)meptazinol opioid analgesics arylpiperidines Conformational Analysis,pharmacophore.

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严重急性呼吸道综合征冠状病毒疫苗研究现状

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生物化学与生物物理进展 2003年第3期30卷 331-334页

作者：叶迅孟夏董继斌梁旻胡放陈红专上海第二医科大学药理学教研室上海200025 复旦大学药学院生物化学教研室上海200032 上海三维生物技术有限公司

冠状病毒被认为是新近爆发的严重急性呼吸道综合征的病原体 .迄今公共数据库中已发表了不同国家和地区分离的 12个SARS病毒株基因组完整序列 .突起蛋白是冠状病毒的主要抗原 ,包含许多抗原决定簇 .SARS冠状病毒突起蛋白的相对保守性为... 详细信息

冠状病毒被认为是新近爆发的严重急性呼吸道综合征的病原体 .迄今公共数据库中已发表了不同国家和地区分离的 12个SARS病毒株基因组完整序列 .突起蛋白是冠状病毒的主要抗原 ,包含许多抗原决定簇 .SARS冠状病毒突起蛋白的相对保守性为有效疫苗的开发奠定了很好的研究基础 .灭活或减毒的冠状病毒疫苗存在一定的局限性和危险性 .

关键词：严重急性呼吸道综合征冠状病毒疫苗突起蛋白 SARS 基因组

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Deferiprone protects the isolated atria from cardiotoxicity induced by doxorubicin

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Acta Pharmacologica Sinica 2006年第10期27卷 1333-1339页

作者： Ling-jie XU~(1,4) Liang JIN~1 Hong PAN~1 Ao-zhen ZHANG~2 Gang WEI~1 Ping-ping LI~3 Wei-yue LU~(1,5) ~1fudan-PharmCo Drug Targeting Research Center,Department of Pharmaceutics,~2Department of Pharmacology,~3school of pharmacy,fudan university,shanghai 200032,china ~4Department of Pharmaceutics,school of pharmacy,Guiyang Medical university,Guiyang 550004,china fudan-PharmCo Drug Targeting Research Center Department of Pharmaceutics Fudan University Shanghai 200032 China

Aim:To investigate the effects of deferiprone on doxorubicin-inducedcardiotoxicity and determine its protection on cardiac contractility in vivo at tis-sue ***:Spontaneously-beating isolated atria from rats were pre-treated with deferiprone for 10 min at 1.2 mmol/L or 0.3 mmol/L,respectively beforeco-incubation with doxorubicin(DOX)at 0.03 mmol/L for 60 ***(dF/dt)was assessed every 10 min during the *** that,the tissuesaround the sinuatrial nodes were fixed for ultrastructural study;succinate dehy-drogenase(SDH)and Cu,Zn superoxide dismutase(Cu,Zn-SOD)activity,as wellas malondialdehyde(MDA)level of the atria were ***:Treatmentwith DOX alone resulted in a 49.34% reduction of the contractility,mitochondriaswelling,disruption of mitochondrial crista and decreased electron density of ***,with the presence of deferiprone,the negative inotropiceffect and lesions in the cardiac mitochondria structure induced by DOX ***,Zn-SOD activity increased by 12.97%-12.11%,the MDA leveldecreased by 29.12%-39.82% and succinate dehydrogenase(SDH)activity wasameliorated by 25.15%-34.76%.Conclusion:Deferiprone can efficiently pre-serve cardiac ***,the results of this study indicate thatdeferiprone is able to protect mitochondrial function and structure form damageinduced by *** cardiac protective potential of deferiprone could be due toits defense capability against oxidative damage.

关键词： deferiprone doxorubicin cardiotoxicity cardiac protection isolated atria

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丹皮酚的分析方法

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时珍国医国药 2005年第1期16卷 63-64页

作者：柳淑玉张彤柳晨山东省龙口市人民医院 265701 山东省烟台市北海医院 265701 复旦大学医学院上海200032

目的 :概述丹皮酚的定量分析方法 ,为丹皮酚及其复方制剂的质量控制提供参考 ,同时便于药学工作者根据各种实际情况加以选择应用。方法 :对近年来的相关文献进行追踪、整理分类。结果 :归纳出丹皮酚的 9种定量分析方法。结论 :对丹皮酚... 详细信息

目的 :概述丹皮酚的定量分析方法 ,为丹皮酚及其复方制剂的质量控制提供参考 ,同时便于药学工作者根据各种实际情况加以选择应用。方法 :对近年来的相关文献进行追踪、整理分类。结果 :归纳出丹皮酚的 9种定量分析方法。结论 :对丹皮酚的定量分析方法的研究已相当深入和广泛 ,为丹皮酚制剂的质量控制打下良好的基础。

关键词：丹皮酚分析方法

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Role of Apolipoprotein A-I in Protecting against Endotoxin Toxicity

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 419-424页

作者： Juan MA, Xue-Ling LIAO, Bin LOU, and Man-Ping WU* Department of Biochemistry, school of pharmacy, fudan university, shanghai 200032, china Department of Biochemistry School of Pharmacy Fudan UniversityShanghai 200032 China

High density lipoprotein (HDL) binds lipopolysaccharide (LPS or endotoxin) and neutralizes its toxicity. We investigated the function of Apolipoprotein A-I (ApoA-I), a major apolipoprotein in HDL, in this process. Mouse macrophages were incubated with LPS, LPS+ApoA-I, LPS+ApoA-I+LFF (lipoprotein-free plasma fraction d>1.210 g/ml), LPS+HDL, LPS+HDL+LFF, respectively. MTT method was used to detect the mortality of L-929 cells which were attacked by the release-out cytokines in LPS-activated macrophages. It was found that ApoA-I significantly decreased L-929 cells mortality caused by LPS treatment (LPS vs. LPS+ApoA-I, P3.20 ± 10.13) h in LPS+ApoA-I group and (46.80 ± 3.79) h in LPS+ApoA-I+LFF group, which were significantly longer than that in LPS group (16.25 ± 17.28) h (P35 ± 3.70) (P3) ApoA-I is the major contributor for HDL anti-endotoxin function.

关键词： Apolipoprotein-I lipopolysaccharide (LPS) macrophages FITC-lipopolysaccharide (FITC-LPS) L-929 cells

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A New Stilbene Tetramer from Caragana rosea

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Chinese Chemical Letters 2003年第10期14卷 1048-1050页

作者： Guo Xun YANG, Chang Qi HU school of pharmacy, fudan university, shanghai 200032 school of pharmacy Fudan University Shanghai 200032 China

Cararosinol A, a new stilbene tetramer, was isolated from Caragana rosea. Its structure has been established on the basis of spectroscopic evidence.

关键词： Caragana rosea Leguminase stilbene tetramer.

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Identification and Characterization of glnA Promoter and its Corresponding Trans-regulatory Protein GlnR in the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 831-843页

作者： Hao YU~(1&) Wen-Tao PENG~1 Yang LIU~1 Ting WU~2 Yu-Feng YAO~1 Ming-Xue CUI~1 Wei-Hong JIANG~1 Guo-Ping ZHAO~(1,3,4*) 1 Laboratory of Molecular Microbiology,Institute of Plant Physiology and Ecology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 200032,china 2 Department of Medicinal Chemistry,school of pharmacy,fudan university,shanghai 200032,china 3 State Key Laboratory of Genetic Engineering & Department of Microbiology,school of Life Science,fudan university,shanghai 200433,china 4 Laboratory of Disease and Health Genomics,Chinese National Human Genome Center at shanghai,shanghai 201203,china Laboratory of Molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai 200032 China State Key Laboratory of Genetic Engineering & Department of Microbiology School of Life Science Fudan University Shanghai 200433 China Laboratory of Disease and Health Genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China

The genetic requirements for the transcription of glnA,encoding the major glutaminesynthetase in a rifamycin SV-producing Amycolatopsis mediterranei strain,U32,were *** experiments showed that the promoter of U32 glnA （pglnA） was likely to have two transcriptioninitiation sites:P1and P2,located 157 and 45 nucleotides （nt） upstream of the translational start codon,*** mobility shift and DNase I footprinting analyses revealed a 30 bp cis-element locatedat 45 to 75 nt downstream of P1,or 38 to 68 nt upstream of *** sequence of the cis-element displayedhigh similarity to the corresponding regions of pglnA from Streptomyces coelicolor and *** as a reporter gene,the expression levels of U32 pglnA and its deletion derivatives under differentnitrogen-source conditions were analyzed by detecting the catechol dioxygenase activities in ***54,*** J508 and *** FS10 （glnR mutant）.These in vivo studies showed that theactivation of U32 pglnA in *** required GlnR,and its binding to the U32 pglnA was furtherconfirmed by the gel mobility shift *** and heterologous expression of the U32 glnR allowed usto detect the in vitro interaction between the U32 GlnR and the corresponding pglnA *** shown by in vivo glnR inactivation and complementation indicated that GlnR is essential for theactive transcription of glnA in U32.

关键词： glnA promoter cis-element glnR Amycolatopsis mediterranei

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Distribution of nimodipine in brain following intranasal administration in rats

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Acta Pharmacologica Sinica 2004年第4期25卷 124-129页

作者： Qi-zhi ZHANG, Xin-guo JIANG2, Chun-hua WU Department of Pharmaceutics, school ofpharmacy, fudan university, shanghai 200032, china Department of Pharmaceutics School of Pharmacy Fudan University

AIM: To determine whether nasally applied nimodipine (NM) could improve its systemic bioavailability and be transported directly from the nasal cavity to the brain. METHODS: NM was administered nasally, intravenously (iv), and orally to male Sprague-Dawley rats. At different times post dose, blood, cerebrospinal fluid (CSF), and brain tissue samples were collected, and the concentrations of NM in the samples were analyzed byHPLC. RESULTS: Oral systemic bioavailability of NM in rats was 1.17 %, nasal dosing improved bioavailibility to 67.4 %. Following intranasal administration, NM concentrations in olfactory bulb (OB) within 30 min post dose were found significant higher than in the other brain tissues. However, similar NM levels in different brain regions were observed after iv injection. AUC in CSF and OB from the nasal route was 1.26 and 1.39 fold compared with the iv route, respectively. The brain-to-plasma AUC ratios were significantly higher after nasal administration than after iv administration (P<0.01). CONCLUSION: Nasally administered NM could markedly improve the bioavailability and a fraction of the NM dose could be transported into brain via the olfactory pathway in rats.

关键词： intranasal administration nimodipine olfactory bulb olfactory pathways

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Impact of Long-term Exposure to Air Particulate Matter on Life Expectancy and Survival Rate of shanghai Residents

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Biomedical and Environmental Sciences 2002年第3期15卷 209-214页

作者： KAN HAI-DONG1 AND CHEN BING-HENG Department of Environmental Health, school of Public Health, fudan university, shanghai 200032, china Department of Environmental Health School of Public Health Fudan University 上海 200032

Objective To evaluate the impact of long-term air particulate matter exposure on the life expectancy and survival rate of shanghai residents. Methods Epidemiology - based exposure-response function was used for the calculation of attributable deaths to air particulate matter in shanghai, and the effect of long-term exposure to particulate matter on life expectancy and survival rate was estimated using the life table of shanghai residents in 1999. Results It was shown that in 1999, the long-term air particulate matter exposure caused 1.34-1.69 years reduction of life expectancy and a decrease of survival rate for each age group of shanghai residents. Conclusion The effect of long-term exposure to air particulate matter on life expectancy is substantial in shanghai.

关键词： Particulate matter Life expectancy Survival rate shanghai

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