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Stable Skin-specific Overexpression of Human CTLA4-Ig in Transgenic Mice through Seven Generations

Acta Biochimica et Biophysica Sinica 2006年第3期38卷 171-178页

作者： Yong WANG~1 Yong NI~(1,3) Hong WEI~1* Feng-Chao WANG~2 Liang-Peng GE~1 Xiang GAO~41 Department of Animal Science,College of Basic Medicine,Third Military Medical university,chongqing 400038,china 2 Institute of Combined Injury,College of Military Preventive Medicine,Third Military Medical university,chongqing 400038,china 3 school of life sciences,southwest university,chongqing 400715,china 4 Animal Model Research Institute,Nanjing university,Nanjing 210093,china Department of Animal Science College of Basic Medicine Third Military Medical UniversityChongqing 400038 China school of life sciences Southwest UniversityChongqing 400715 China Institute of Combined Injury College of Military Preventive Medicine Third Military Medical UniversityChongqing 400038 China Animal Model Research Institute Nanjing UniversityNanjing 210093 China

Skin graft rejection is a typical cellular immune response,mainly mediated by T *** lymphocyte associated antigen 4-immunoglobin (CTLA4-Ig) extends graft survival by blocking the T cellco-stimulation pathway and inhibiting T cell *** investigate the efficacy of CTLA4-Ig in prolong-ing skin graft survival,human CTLA4-Ig (hCTLA4-Ig) was engineered to overexpress in mouse skin bytransgenesis using the K14 *** transcription-polymerase chain reaction (RT-PCR) and West-ern blot assay indicated that the expression of CTLA4-Ig remained skin-specific and relatively constantcompared to the internal control protein,AKT,through seven *** presence and concentrationof the hCTLA4-Ig protein in transgenic mouse sera was determined by enzyme-linked immunosorbent assay(ELISA),and the results indicated that the serum CTLA4-Ig concentration also remained constant *** of transgenic mouse skins grafted onto rat wounds was remarkably prolonged com-pared to that of wild-type skins from the same mouse strain,and remained comparable among all *** suggested that the bioactive hCTLA4-Ig protein was stably expressed in transgenical micethrough at least seven generations,which was consistent with the stable skin-specific CTLA4-Ig *** results demonstrated that the transgenic expression of hCTLA4-Ig in skin driven by the K14 promoterremained constant through generations,and a transgenic line can be established to provide transgenic skinwith extended survival reproducibly.

关键词： cytotoxic T lymphocyte antigen 4-Ig transgenic mice skin K14 promoter

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重庆外来入侵植物调查研究

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西南师范大学学报（自然科学版） 2004年第5期29卷 863-866页

作者：石胜璋田茂洁刘玉成重庆林业规划设计院重庆400060 西华师范大学生命科学学院四川南充637002 西南师范大学生命科学学院重庆400715

经多年调查积累和资料查阅,发现重庆市有外来入侵植物46种39属21科.其中具有严重危害性植物3种(空心莲子草、凤眼莲和红花酢浆草),较严重危害性植物6种,一般影响植物37种.重庆已发现紫茎泽兰,应尽早清除,否则后果严重.同时简略讨论了外... 详细信息

经多年调查积累和资料查阅,发现重庆市有外来入侵植物46种39属21科.其中具有严重危害性植物3种(空心莲子草、凤眼莲和红花酢浆草),较严重危害性植物6种,一般影响植物37种.重庆已发现紫茎泽兰,应尽早清除,否则后果严重.同时简略讨论了外来入侵植物的入侵过程,危害作用,控制清除方法.

关键词：重庆外来入侵植物植物危害生态环境

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Theoretical study of bifurcated bent blue-shifted hydrogen bonds CH_2…Y

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Science china Chemistry 2008年第7期51卷 623-631页

作者： LI AnYong school of Chemistry and Chemical Engineering, southwest university, chongqing 400715, china school of Chemistry and Chemical Engineering Southwest University Chongqing China

Ab initio quantum chemistry methods were applied to study the bifurcated bent hydrogen bonds Y… H2CZ (Z = O, S, Se) and Y…H2CZ2 (Z = F, Cl, Br) (Y = Cl-, Br-) at the MP2/6-311++G(d,p) and MP2/6-311++G(2df,2p) levels. The results show that in each complex there are two equivalent blue-shifted H-bonds Y…H—C, and that the interaction energies and blue shifts are large, the energy of each Y…H—C H-bond is 15-27 kJ/mol, and Δr(CH) = -0.1 - -0.5 pm and Δv(CH) = 30 - 80 cm-1. The natural bond orbital analysis shows that these blue-shifted H-bonds are caused by three factors: large rehybridization; small direct intermolecular hyperconjugation and larger indirect intermolecular hy- perconjugation; large decrease of intramolecular hyperconjugation. The topological analysis of elec- tron density shows that in each complex there are three intermolecular critical points: there is one bond critical point between the acceptor atom Y and each hydrogen, and there is a ring critical point inside the tetragon YHCH, so these interactions are exactly H-bonding.

关键词： bifurcated bent H-bonds blue-shifted H-bonds topological property of electron density intramolecular hyperconjugation

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Size effect of fullerene cages and encaged clusters in M3N@C2n

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Science china Chemistry 2009年第5期52卷 584-589页

作者： GAN LiHua school of Chemistry and Chemical Engineering, southwest university, chongqing 400715, china school of Chemistry and Chemical Engineering Southwest University Chongqing China

Based on the calculated findings that the sizes of encaged clusters determine the structures and the stability of C80-based trimetallic nitride fullerenes (TNFs), more extensive density functional theory calculations were performed on M3N@C68, M3N@C78 and M3N@C80 (M=Sc, Y and La). The calculated results demonstrated that the structures and stability undergo a transition with the increasing of the sizes of the cages and clusters. Sc3N is planar inside the three considered cages, Y3N is slightly pyramidal inside C68-6140 and C78-5 and planar inside I h C80-7, however, La3N is pyramidal inside all the three cages. Those cages with pyramidal clusters inside deformed considerably, compared with their parent cages. In these cases, the bonding of metallic atoms toward the cages does not play an impor-tant role, and the encaged cluster tends to be located inside the cages with the largest M-M and M-C distances so that the strain energy can be released mostly. These calculations revealed the size effect of fullerene cages and encaged clusters, and can explain the position priority of M3N inside fullerene cages and the differences in yield of M3N@C2n.

关键词： trimetallic nitride fullerenes (TNFs) density functional theory (DFT) size effect stability

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Nitrogen content features of several indigenous evergreen and decidous tree species in chongqing region

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西南师范大学学报(自然科学版) 1999年第5期24卷 586-593页

作者： Zeng\ Bo school of life Science,southwest china Normal university,chongqing 400715 西南师范大学重庆

To aid to reveal the basic physiological differences existing between evergreen and deciduous plants, so as to facilitate our understanding of relationships between physiological features of evergreen & deciduous plants and their corresponding growth and biomass production, nitrogen content patterns of four indigenous tree species in chongqing region including evergreen and deciduous plants growing in optimal conditions were analyzed. It is shown that leaf nitrogen content of deciduous tree species was significantly higher than that of evergreen tree species. Based on this result, it can be inferred that researched evergreen and deciduous tree species had basic differences in their leaf nitrogen content. There were no significant differences appeared between studied evergreen and deciduous tree species on nitrogen content of branches and stems. Compared with branch and stem, leaves had highest nitrogen content unexceptionally for all researched plants regardless of species. Nitrogen content of stems decreased with the age of stem for all investigated evergreen and deciduous species.

关键词： evergreen deciduous tree species nitrogen content chongqing

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Construction and cross-correlation analysis of the affective physiological response database

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Science china(Information sciences) 2010年第9期53卷 1774-1784页

作者： WEN WanHui1,QIU YuHui2,LIU GuangYuan1*,CHENG NanPu3 & HUANG XiTing4 1school of Electronic and Information Engineering,southwest university,chongqing 400715,china 2College of Computer and Information Science,southwest university,chongqing 400715,china 3school of Materials Science and Engineering,southwest university,chongqing 400715,china 4school of Psychology,southwest university,chongqing 400715,china school of Electronic and Information Engineering Southwest University Chongqing China College of Computer and Information Science Southwest University Chongqing China school of Materials Science and Engineering Southwest University Chongqing China school of Psychology Southwest University Chongqing China

Six kinds of emotions,namely joy,surprise,disgust,grief,anger and fear,are elicited from 300 ordinary college students by means of high-rated movie *** signals of galvanic skin response(GSR),heart rate(HR),blood volume pulse(BVP),electrocardiogram(ECG),respiration(Rsp.),facial electromyography(EMG) and two channels of electroencephalography(EEG) from the frontal lobe were recorded while the subjects were watching the affective eliciting *** samples of each kind of signals are analyzed by using random matrix theory(RMT).From the distribution of the eigenvalues and eigenvectors of the correlation matrices of BVP,facial EMG and two EEGs,we find that the statistical properties of these four kinds of signals coincide with the RMT ***,the largest eigenvalue and the corresponding eigenvector component distribution of the correlation matrices of GSR,HR,ECG and *** deviated significantly from the RMT *** experiments also reveal that the correlations between two arbitrary samples of BVP,facial EMG or EEGs does not result from affective response but from random noises,whereas the amplitude variations of GSR,HR,ECG and *** the correlated affective physiological response *** to the data analysis results of RMT,110 features are firstly extracted from the GSR and HR signals,and then a subset of the initial features is selected by using the Backward Selection *** affective data samples each of which is a vector of the features are classified by using a Fisher classifier,and furthermore,the user-independent emotion recognition systems with good prediction performance are constructed during the model selection process.

关键词： affective computing emotion recognition affective physiological response database correlation analysis random matrix theory

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Identification of incompatible groups in sweet potato( Ipomoea batatas (L.) Lam.) with RAPD markers

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西南师范大学学报(自然科学版) 1999年第5期 581-585页

作者： Sun\ Min school of life Sciense,southwest china Normal university,chongqing 400715

RAPD (Random Amplified Polymorphic DNA) analysis was applied to sweet potato ( Ipomoea batatats (L.) Lam.). In order to assess the degree of polymorphism with the incompatible groups and to investigate if this approach was suitable for identifying incompatible groups, sixteen sweet potato cultivars and one related wild species? 獻.trifida , including 7 incompatible groups, were evaluated by using random 10-mer primers. Wide variations in banding profiles among cultivars were observed and cluster analysis was used to generate a dendrogram showing the relationships among incompatible groups of sweet potato.

关键词： Incompatible group RAPD marker Ipomoea batatas

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An isoperimetric deficit upper bound of the convex domain in a surface of constant curvature

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Science china Mathematics 2010年第8期53卷 1941-1946页

作者： LI Ming & ZHOU JiaZu school of Mathematics and Statistics,southwest university,chongqing 400715,china school of Mathematics and Statistics Southwest University Chongqing China

In this paper,we give a reverse analog of the Bonnesen-style inequality of a convex domain in the surface X of constant curvature,that is,an isoperimetric deficit upper bound of the convex domain in *** result is an a... 详细信息

关键词： Kinematic formula the surface of constant curvature isoperimetric deficit convex set

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How to Protect the Karst Aquifers?

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地学前缘 2009年第S1期16卷 32-33页

作者： Junbing Pu,Daoxian Yuan,Yongjun Jiang school of Geographical sciences,southwest university,chongqing 400715,china.

The karst aquifers are the most important water resources in southwest china,where displays the most continuous bare karst around the world and hold about 100 million *** the rapid expansion of the urbanization,development of the industry and agriculture,the groundwater quality has been *** of the karst groundwater increases because of development of intensive *** of nitrate and sulphate increase notably as a result of the large amount of chemical fertilizer used in *** gas, waste water and waste residues produced in the processes of industrialization and urbanization cause groundwater acidification and an increase of pollutants such as nitrogen,phosphate,chloride,

关键词： karst aquifers groundwater quality -contamination protection measures

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The resonance Rayleigh scattering of the interaction of copper(Ⅱ)-bleomycinA2 with DNA and its analytical application

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Science china Chemistry 2010年第3期53卷 618-624页

作者： LIU JiangTao1,LIU ShaoPu2 & LIU ZhongFang2 1school of Chemistry,chongqing Normal university,chongqing 400300,china 2school of Chemistry and Chemical Engineering,southwest university,chongqing 400715,china school of Chemistry Chongqing Normal University Chongqing China school of Chemistry and Chemical Engineering Southwest University Chongqing China

The forming of bleomycinA2-Cu(Ⅱ) cationic chelate and the interaction of the chelate with DNA have been investigated by using resonance Rayleigh scattering(RRS),molecular absorption and fluorescence *** result shows that in aqueous solution,bleomycinA2(BLMA2) can react with Cu(Ⅱ) to form 1:1 cationic chelate which contributes to the changes of the absorption spectra and the quenched fluorescence of *** the cationic chelate further bound with DNA to form ternary ion-association complexes,the remarkable enhancement of the RRS intensity was *** this work,the optimum conditions for the coordination reaction of BLMA2 with Cu(Ⅱ) and some influencing factors have been *** reaction mechanism of BLMA2-Cu(Ⅱ) binding with DNA was suggested and a binding model was *** addition,the fluorescence quenching type of BLMA2 was investigated.A highly sensitive,simple and rapid new method for the determination of DNA by using BLMA2-Cu(Ⅱ) as RRS probe has been *** detection limits(3σ) are 7.2 ng/mL for ctDNA,7.1 ng/mL for sDNA and 18 ng/mL for *** method can be applied to the determination of trace amounts of DNA.

关键词： bleomycinA2 Cu(Ⅱ) DNA resonance Rayleigh scattering fluorescence quenching

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