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Recombinant Neural Protein PrP Can Bind with Both Recombinant and Native Apolipoprotein E In Vitro

Acta Biochimica et Biophysica Sinica 2006年第9期38卷 593-601页

作者： Chen GAO~1 Yan-Jun LEI~(1,2) Jun HAN~1 Qi SHI~1 Lan CHEN~(1,3) Yan GUO~(1,4) Yong-Jun GAO~1 Jian-Ming CHEN~1 Hui-Ying JIANG~1 Wei ZHOU~1 Xiao-Ping DONG~1* 1 state key laboratory for infectious disease prevention and control,national institute for viral disease control and prevention,chinese center for disease control and prevention,beijing 100052,china 2 School of Medicine,Xi'an Jiaotong University,Xi'an 710061,china 3 national laboratory of Medical Molecular Biology,institute of Basic Medical Science,chinese Academy of Medical Sciences and Peking Union Medical College,beijing 100005,china 4 College of Science and Veterinary Medicine,Northwest Agriculture and Forest University,Yangling 712100,china statekeylaboratoryforinfectiousdiseasepreventionandcontrol NationalInstituteforViralDiseaseControlandPreventionChineseCenterforDiseaseControlandPreventionBeijing100052China SchoolofMedicine Xi'anJiaotongUniversity

The most essential and crucial step during the pathogenesis of transmissible spongiformencephalopathy is the conformational change of cellular prion protein （PrPC） to pathologic isoform (PrPSc).Alot of data revealed that caveolae-like domains （CLDs） in the cell surface were the probable place where theconversion of PrP proteins *** E （ApoE） is an apolipoprotein which is considered toplay an important role in the development of Alzheimer's disease and other neurodegenerative diseases byforming protein complex through binding to the receptor located in the clathrin-coated pits of the cell *** this study,a 914-bp cDNA sequence encoding human ApoE3 was amplified from neuroblastoma cell *** human ApoE isomers were expressed and purified from Escherichia ***-specificantiserum was prepared by immunizing rabbits with the purified ***/His pull-down assay,immunoprecipitation and ELISA revealed that three full-length ApoE isomers interact with the recombinantfull-length PrP protein in *** regions corresponding to protein binding were mapped in the N-terminalsegment of ApoE （amino acid 1-194） and the N-terminal of PrP （amino acid 23-90）.Moreover,the recombinantPrP showed the ability to form a complex with the native ApoE from liver *** data provided directevidence of molecular interaction between ApoE and *** also supplied scientific clues for assessing thesignificance of CLDs on the surface of cellular membrane in the process of conformational conversion fromPrPCto PrPScand probing into the pathogenesis of transmissible spongiform encephalopathy.

关键词： prion disease PrP apolipoprotein E protein interaction caveolae-like domain

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Molecular characteristics of Vibrio cholerae O139 isolated from Diarrheal patients and River water,Guangdong,china

Molecular characteristics of Vibrio cholerae O139 isolated f...

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2010广东省预防医学会学术年会

作者： Bai-sheng LI~1,Hai-ling TAN~1,Duo-chun WANG~2,Xiao-ling DENG~(1*),Jing-diao CHEN~1,Hao-jie ZHONG~1,Bi-xia KE~1,Chang-wen KE~1,Biao Kan~2. institute of Microbiology,center for diseases control and prevention of Guangdong Province,Guangzhou 510300,china 1 state key laboratory for infectious disease prevention and control,national institute of Communicable disease control and prevention,chinese center of disease control and prevention,beijing 102206,china 2

Aims:To enhance the understanding of epidemiological impact of environmental *** 0139 strains, we characterized 10 clinical and 20 environmental isolates collected from human clinical samples and Pear River estuary during 2006 to 2008. Methods and Results:Isolates were tested by PCR for eight virulence genes:ctxA,zot,ace,st,hlyA,tcpA, tcpI and *** relatedness was assessed by PFGE and antimicrobial susceptibility was determmed using disk *** of eight virulence markers were detected in six clinical isolates and one environmental *** clinical and one environmental isolate were positive for six virulence markers. 60%clinical isolates showed simultaneous resistance to TET,NAL,CHL and AMP,70%were resistant to SXT,while only 35%environmental strains were resistant to *** analysis revealed that the isolates in this study were formed three ***Ⅲwith more relate to strains from diarrheal patients than the strains in other clusters. Conclusions:Different from the clinical strains,most environmental strains lacked CTX and TCP gene *** environmental strains present single antibiotic resistant,while most clinical isolates show multidrug *** analysis indicated the clusterⅢhas more possibility to become a potential pathogenic clonal cluster. Significance and Impact of Study:This study would be of value in risk assessment for human health.

关键词： Antibiotics Molecular epidemiology PCR virulence PFGE

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中国2013年脊髓灰质炎实验室网络的运转状况与评价

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中国疫苗和免疫 2015年第3期21卷 263-268页

作者：朱晖祝双利张勇王东艳严冬梅李晓嫘安洪秋许文波中国疾病预防控制中心病毒病预防控制所卫生部医学病毒学和病毒病重点实验室世界卫生组织西太平洋区脊髓灰质炎参比实验室北京102206

目的通过对中国(未包括香港、澳门特别行政区和台湾地区,下同)2013年脊髓灰质炎(脊灰)实验室网络(Polio laboratory Network,PLN)监测数据和各项监测指标的分析和评估,为维持我国无脊灰状态提供病毒学依据。方法对中国免疫规划监测信息... 详细信息

目的通过对中国(未包括香港、澳门特别行政区和台湾地区,下同)2013年脊髓灰质炎(脊灰)实验室网络(Polio laboratory Network,PLN)监测数据和各项监测指标的分析和评估,为维持我国无脊灰状态提供病毒学依据。方法对中国免疫规划监测信息管理系统数据库中,31个省(自治区、直辖市,下同)报告的急性弛缓性麻痹(Acute Flaccid Paralysis,AFP)病例个案调查表和中国疾病预防控制中心(center for disease control and prevention,CDC)病毒病预防控制所国家脊灰实验室(national Polio laboratory,NPL)的监测数据库进行统计分析,对PLN的各项运转指标进行评价。结果中国2013年PLN收集到5623例AFP病例的11 126份粪便标本,14天内双份粪便标本采集率为92.78%,合格粪便标本采集率为92.35%,鉴定结果 28天内反馈率为99.10%。2013年从5623例粪便标本中有111例分离到脊灰病毒(Poliovirus,PV),分离率为1.97%;606例粪便标本分离到非脊灰肠道病毒(Non-polio Enterovirus,NPEV),分离率为10.78%。2013年,NPL收到PLN送检的237份PV分离物,按单个血清型定型统计共计304株,其中19株为疫苗衍生(Vaccine-derived)PV(VDPV),1株来源于1例非AFP病例,另18株来源于1例免疫缺陷相关的(Immunodeficiency-associated)VDPV(i VDPV)病例;未发现野生型(Wild)PV(WPV)。2013年,NPL和31个省CDC脊灰实验室都通过了世界卫生组织(World Health Organization)粪便标本PV等EV的新检测流程的盲样标本考核,NPL和其中8个省CDC脊灰实验室接受并通过了WHO现场评估认证,其他省级CDC脊灰实验室通过信函认证表格认证。结论中国PLN 2013年运转正常,未发现WPV或WPV病例,为维持我国无脊灰状态,早期发现VDPV或VDPV病例,早期采取应对措施阻断其传播提供了重要的病毒学依据。

关键词：脊髓灰质炎实验室网络运转状况脊髓灰质炎病毒疫苗衍生脊髓灰质炎病毒

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C群和不可分群脑膜炎奈瑟菌对人喉癌上皮细胞黏附能力的研究

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中国疫苗和免疫 2012年第3期18卷 211-216页

作者：杨郝亮秦天徐丽任红宇朱兵清邵祝军中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室北京102206

目的建立和优化脑膜炎奈瑟菌(Neisseria Meningitidis,Nm)对人喉癌上皮细胞(Human Larynx Carcinoma Epithelial Cell,HEp-2)的黏附实验方法,检测C群序列型(SequenceType,ST)4821序列群和不可分群(Nongroupable,NG)Nm对HEp-2黏附能力的差异性。方法以C群Nm疫苗株C11作为靶菌,选用HEp-2,选择不同的黏附时间和不同的感染复数(Multiplicity of Infection,MOI)确定Nm与HEp-2黏附作用的最适条件,对中国分离的26株C群ST-4821序列群和8株NG Nm菌株进行HEp-2黏附能力研究。结果 Nm与HEp-2黏附研究最适作用时间为4h,最适MOI为100～1000;各血清群参考菌株的黏附菌落数在1.46×105～1.63×106菌落形成单位/毫升(Colony Form ingUnit,CFU/ml);26株ST-4821序列群的C群Nm对HEp-2的黏附菌落数在1.33×105～9.53×106CFU/ml;8株NGNm的黏附菌落数在3.04×105～8.52×106CFU/ml。结论不同血清群Nm对HEp-2的黏附能力存在差异;同是ST-4821序列群的C群Nm对HEp-2的黏附能力存在差异;同是NG Nm对HEp-2的黏附能力存在差异。

关键词：脑膜炎奈瑟菌黏附能力人喉癌上皮细胞

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Study on interaction between microtubule associated protein tau and prion protein

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Science china(Life Sciences) 2006年第5期49卷 473-479页

作者： HAN Jun1,ZHANG Jin1,2,YAO Hailan1,3,WANG Xiaofan1,LI Feng1,CHEN Lan1,4,GAO Chen1,GAO Jianmei1,NIE Kai1,ZHOU Wei1 & DONG Xiaoping11.state key laboratory for infectious diseases prevention and control,national institute for viral disease control and Pre-vention,chinese center for disease control and prevention,beijing 100052,china 2.School of Medicine,Xi’an Jiao-Tong University,Xi’an 710061,china 3.Tong-Ji Medical College,Hua-Zhong University of Science and Technology,Wuhan 430030,china 4.national laboratory of Medical Molecular Biology,institute of Basic Medical Science,chinese Academy of Medical Sciences and Peking Union Medical College,beijing 100005,china 1. state key laboratory for infectious diseases prevention and control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China 2. School of Medicine Xi’an Jiao-Tong University Xi’an 710061 China 3. Tong-Ji Medical College Hua-Zhong University of Science and Technology Wuhan 430030 China 4. national laboratory of Medical Molecular Biology Institute of Basic Medical Science Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100005 China

Microtubule-associated protein tau is considered to play roles in many neurodegenera-tive diseases including some transmissible spongiform *** address the possible molecular linkage of prion protein(PrP) and tau,a GST-fusion segment of human tau covering the three-repeat region and various PrP segments was used in the tests of GST pull-down and *** found tau protein interacted with various style prion proteins such as native prion protein(PrPC) or protease-resistant isoform(PrPSc) .Co-localization signals of tau and PrP were found in the CHO cell tranfected with both PrP and tau *** domain of interaction with tau was located at N-terminal of PrP(residues 23 to 91) .The evidence of molecular interactions between PrP and tau protein highlights a potential role of tau in the biological function of PrP and the pathogenesis of TSEs.

关键词： tau, PrP, interaction.

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浅析支原体污染对手足口病相关肠道病毒分离率的影响

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中国疫苗和免疫 2014年第3期20卷 230-232页

作者：檀晓娟冀天娇张宝敏王东艳许文波中国疾病预防控制中心病毒病预防控制所卫生部医学病毒学和病毒病重点实验室世界卫生组织西太平洋区脊髓灰质炎参比实验室北京102206

目的探讨支原体污染对手足口病(Hand,Foot and Mouth disease,HFMD)相关肠道病毒(Enterovirus,EV)分离率的影响。方法随机选择13个省的HFMD相关EV培养物共26份,同时从EV复核率较低的某省送检的HFMD相关EV培养物中,随机挑选18份,采用套... 详细信息

目的探讨支原体污染对手足口病(Hand,Foot and Mouth disease,HFMD)相关肠道病毒(Enterovirus,EV)分离率的影响。方法随机选择13个省的HFMD相关EV培养物共26份,同时从EV复核率较低的某省送检的HFMD相关EV培养物中,随机挑选18份,采用套式聚合酶链反应(Nested Polymerase Chain Reaction,nPCR)对细胞培养物中的支原体核酸进行检测。对阳性产物进行序列测定,通过基本多序列比对工具(Basic Local Alignment Search Tool,BLAST)对序列进行同源性比对。结果随机挑选13个省的EV培养物中,4个省的标本支原体检测结果为阴性,1个省同时检出猪鼻支原体和精氨酸支原体,8个省检出精氨酸支原体。EV复核率较低省送检的18份培养物中均检出牛支原体。结论支原体污染在中国HFMD实验室使用的细胞系中普遍存在,某些支原体的污染,可能降低细胞的敏感性,引起病毒分离率的下降。避免细胞系的支原体污染对提高HFMD实验室网络运转质量具有重要意义。

关键词：手足口病肠道病毒支原体套式聚合酶链反应

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Molecular cloned SHIV-CN97001:a Replication-Competent,R5 Simian-Human Immunodeficiency Virus Containing Env of a Primary chinese HIV-1 Clade C isolate

Molecular cloned SHIV-CN97001:a Replication-Competent,R5 Sim...

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第五次全国免疫诊断暨疫苗学术研讨会

作者： Qiang Liu~(1,2),Yue Li~3,GuiBo Yang~1,JieJie Dai~4,Ruth M Ruprecht~(5,6),YiMing Shao~ 1 national center for AIDS/STD control and prevention,china CDC,beijing,china 2 national institutes for Food and Drug control,beijing,china 3 College of Lifesciences,Nankai University,Tianjin,china 4 institute of Medical Biology,CAMS,Kuming,china 5 Dana-Farber Cancer institute,Boston,Massachusetts, USA 6Harvard Medical School,Boston,Massachusetts,USA

The increasing prevalence of human immunodeficiency virus type 1(HIV-1) subtype C infection worldwide calls for efforts to develop a relevant animal model for evaluating AIDS candidate *** china,the prevalent HIV strains comprise a circulating recombinant form,BC(CRF07C),in which the envelope belongs to subtype *** evaluate potential AIDS vaccines targeting chinese viral strains in nonhuman primate models,we constructed a simian-human immunodeficiency virus(SHIV) carrying most of the envelope sequence of a primary HIV-1 clade C strain isolated from an HIV-positive intravenous drug user from YunNan province in *** of six chinese rhesus macaques with SHIV-CN97001 resulted in a low level of viremia and no significant alteration in CD4+T-lymphocyte *** determine whether in vivo adaptation would enhance the infectivity of SHIV-CN97001,the parental infectious strain was serially passaged through eight chinese rhesus *** hallmarks of SHIV infectivity developed gradually, as shown by the increasingly elevated peak viremia with each *** findings establish that the R5-tropic SHIV-CN97001 /chinese rhesus macaque model should be very useful for evaluation of HIV-1 subtype C vaccines in china.

关键词： Clade C Passage Rhesus macaques SHIV

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Multidimensional dynamic prediction model for hospitalized patients with the omicron variant in china

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infectious disease Modelling 2023年第4期8卷 1097-1107页

作者： Yujie Chen Yao Wang Jieqing Chen Xudong Ma Longxiang Su Yuna Wei Linfeng Li Dandan Ma Feng Zhang Wen Zhu Xiaoyang Meng Guoqiang Sun Lian Ma Huizhen Jiang Chang Yin Taisheng Li Xiang Zhou china national Critical Care Quality control center Group Department of Critical Care Medicine Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China Yidu Cloud Technology Inc. BeijingChina Information center Department State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing100730China Department of Medical Administration National Health Commission of the People's Republic of ChinaBeijing100044China Information center State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China Department of infectious diseases Peking Union Medical College HospitalChinese Academy of Medical SciencesBeijingChina national institute of Hospital Administration BeijingChina

Purpose:To establish dynamic prediction models by machine learning using daily multidimensional data for coronavirus disease 2019(COVID-19)***:Hospitalized COVID-19 patients at Peking Union Medical College Hospital from Nov 2nd,2022,to Jan 13th,2023,were enrolled in this *** outcome was defined as deterioration or recovery of the patient's ***,comorbidities,laboratory test results,vital signs,and treatments were used to train the *** predict the following days,a separate XGBoost model was trained and *** Shapley additive explanations method was used to analyze feature ***:A total of 995 patients were enrolled,generating 7228 and 3170 observations for each prediction *** the deterioration prediction model,the minimum area under the receiver operating characteristic curve(AUROC)for the following 7 days was 0.786(95%CI 0.721-0.851),while the AUROC on the next day was 0.872(0.831-0.913).In the recovery prediction model,the minimum AUROC for the following 3 days was 0.675(0.583-0.767),while the AUROC on the next day was 0.823(0.770-0.876).The top 5 features for deterioration prediction on the 7th day were disease course,length of hospital stay,hypertension,and diastolic blood *** for recovery prediction on the 3rd day were age,D-dimer levels,disease course,creatinine levels and corticosteroid ***:The models could accurately predict the dynamics of Omicron patients’conditions using daily multidimensional variables,revealing important features including comorbidities(e.g.,hyperlipidemia),age,disease course,vital signs,D-dimer levels,corticosteroid therapy and oxygen therapy.

关键词： COVID-19 Omicron Prediction model Machine learning

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Guidelines for the Diagnosis and Treatment of Dengue in china

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infectious diseases & Immunity 2021年第3期1卷 144-152页

作者： Fuchun Zhang Jianfeng He Jie Peng Xiaoping Tang Chengfeng Qin Hongzhou Lu Xingwang Li Qingquan Liu Hong Zhao Jifang Sheng Guiqiang Wang Society of infectious diseases,Society of Tropical Medicine and Parasitology,chinese Medical Association Society of Emergency Medicine,china Association of chinese Medicine Department of infectious diseases Guangzhou Eighth People’s HospitalGuangzhou Medical UniversityGuangzhou 510060China Guangdong Provincial center for disease control and prevention Guangzhou 511430China Department of infectious diseases Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China Department of Virology State Key Laboratory of Pathogen and BiosecurityBeijing Institute of Microbiology and EpidemiologyAcademy of Military Medical SciencesBeijing 100071China Shanghai Public Health Clinical center Shanghai 201508China Department of infectious diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China beijing Hospital of Traditional chinese Medical Beijing 100010China Department of infectious diseases Center for Liver DiseasesPeking University First HospitalBeijing 100034China Department of infectious diseases The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310003China. 不详

Dengue is the most prevalent and rapidly spreading mosquito-borne viral *** a dengue non-endemic country,china has experienced several dengue outbreaks in recent ***,dengue patients in china display clinical characteristics that are distinct from those of patients in dengue-endemic *** standardize the diagnosis and treatment of dengue fever,the experts at the Society of infectious diseases,Society of Tropical Medicine and Parasitology of the chinese Medical Association,and the Society of Emergency Medicine of china Association of chinese Medicine have developed a guideline after reviewing the following guidelines and data:guidelines for diagnosis,treatment,prevention,and control of dengue(World Health Organization,2009);the guidelines for diagnosis and treatment of dengue(national Health and Family Planning Commission of the People’s Republic of china,2014,Edition 2);health industry standard of the People’s Republic of china“diagnosis for dengue fever(WS216-2018)”;and systemic reports on *** new guideline includes eight aspects:introduction,terminology,epidemiology and prevention,etiology and pathogenesis,clinical features,diagnosis,treatment,and problems to be solved.

关键词： Dengue Diagnosis Treatment Guideline

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Expert Consensus on Diagnosis and Treatment of End-Stage Liver disease Complicated with Infections

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infectious diseases & Immunity 2022年第3期2卷 168-178页

作者： Qin Ning Tao Chen Guiqiang Wang Dong Xu Yanyan Yu Qing Mao Taisheng Li Lanjuan Li Jun Li Xiaoju Lu Jiabin Li Zhiwei Li Wenhong Zhang Yonghong Xiao Qinghua Meng Yuqiang Mi Jia Shang Yunsong Yu Yingren Zhao Caiyan Zhao Hong Zhao Jianrong Huang Jie Peng Hong Tang Xiaoping Tang Jinhua Hu Bijie Hu Wei Guo Bo Zheng Baiyi Chen Yuexin Zhang Jia Wei Jifang Sheng Zhi Chen Minggui Wang Qing Xie Yuming Wang Fu-Sheng Wang Jinlin Hou Zhongping Duan Lai Wei Jidong Jia chinese Society of infectious disease of chinese Medical Association Department of infectious disease Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of infectious disease Center for Liver DiseasePeking University First HospitalBeijing 100034China Department of infectious diseases Southwest HospitalArmy Medical UniversityChongqing 400038China Department of infectious diseases Peking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730China state key laboratory for Diagnosis and Treatment of infectious diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China Department of infectious disease The First Affiliated Hospital with Nanjing Medical UniversityNanjing 210029China center of infectious diseases West China Hospital of Sichuan UniversityChengdu 610041China Department of infectious diseases First Affiliated Hospital of Anhui Medical UniversityHefei 230031China Department of infectious diseases Shengjing HospitalAffiliated Hospital of China Medical UniversityShenyang 110801China Department of infectious diseases Institute of Infectious DiseasesHuashan HospitalFudan UniversityShanghai 200040China Department of Severe Liver diseases Beijing Youan HospitalCapital Medical UniversityBeijing 100069China Nankai University Second People’s Hospital Tianjin 300071China Department of infectious disease People’s Hospital of Henan ProvinceZhengzhou 450003China Department of infectious diseases Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310020China Department of infectious diseases First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061China Department of infectious diseases Third Affiliated Hospital of Hebei Medical UniversityShijiazhuang 050051China state key laboratory of Organ Failure Research Guangdong Provincial Key Laboratory of Viral Hepatitis ResearchDepartment o

End-stage liver disease(ESLD)is a life-threatening clinical syndrome that markedly increases mortality in patients with *** patients with ESLD,infections can induce or aggravate the occurrence of liver ***,infections are among the most common complications of disease *** is a lack of working procedure for early diagnosis and appropriate management for patients with ESLD complicated by infections as well as local and international guidelines or *** consensus assembled up-to-date knowledge and experience across chinese colleagues,providing data on principles as well as working procedures for the diagnosis and treatment of patients with ESLD complicated by infections.

关键词： Consensus Diagnosis End stage of liver disease Infection Therapy

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