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Mitosin/CENP-F is a conserved kinetochore protein subjected to cyto plasmic dynein-mediated poleward transport

cell Research 2003年第4期13卷 275-283页

作者： ZHEN YE YANG, JING GUO, NING LI, MIN QIAN, SHENG NIAN WANG, XUE LIANG ZHULaboratory of molecular cell biology, Institute of Biochemistry and cell biology, Shanghai Institutes for Biological sciences, Chinese Academy of sciences, 320 Yueyang Road, Shanghai 200031, china 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China

Mitosin/CENP-F is a human nuclear protein transiently associated with the outer kinetochore plate in M phase and is involved in M phase progression. LEK1 and CMF1, which are its murine and chicken orthologs, however, are implicated in muscle differentiation and reportedly not distributed at kinetochores. We therefore conducted several assays to clarify this issue. The typical centromere staining patterns were observed in mitotic cells from both human primary culture and murine, canine, and mink cell lines. A C-terminal portion of LEK1 also conferred centromere localization. Our analysis further suggests conserved kinetochore localization of mammalian mitosin orthologs. Moreover, mitosin was associated preferentially with kinetochores of unaligned chromosomes. It was also constantly transported from kinetochores to spindle poles by cytoplasmic dynein. These properties resemble those of other kinetochore proteins important for the spindle checkpoint, thus implying a role of mitosin in this checkpoint. The

关键词： mitosin LEKl CENP-F kinetochore.

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Effect of Lanthanum on Growth and Extracellular Enzyme Acitivty of Erwinia Chrysanthemi in vitro

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Journal of Rare Earths 1999年第2期17卷 64-67页

作者： Liu Qingdu (School of Life science, university of science and technology of china, hefei 2.002., china) Zhang Jian , Cheng Heyuan (Department of Plant Protection, Anhui Agricultural university, hefei 230036, china)

The effect of lanthanum on the growth and extracellular enzyme activity of Erwinia chrysanthemi(Ech) was studied. Lanthanum inhibited the growth of Ech in solid culture at the concentration of 50, 100, 150, 200, 250, 300 and 350 mgL-1, and the inhibition increased with the increase of the RE concentration. The formation of colony delayed and the diameter of colony decreased. Lanthanum stimulated the growth of Ech within 24h when the lanthanum concentration was less than 200 mgL-1 in liquid medium, but significantly inhibited the growth of Ech with the increase of lanthanum concentration and culture time. When the concentration was higher than 350 mgL-1, the growth of Ech was inhibited completely both in solid and liquid culture. The extracellular enzyme acitivity of Ech was affected by lanthanum at 200 mgL-1. The activity of cellulose was promoted more than that of protease but the activity of pectinase was decreased. The maceration to potato tuber tissue decreased when the cell free filtrate was tr

关键词： Rare earths Lanthanum Erwinia chrysanthemi Extracellular enzyme Maceration eated with lanthanum.

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Transcription of the putative tumor suppressor gene HCCS1 requires binding of ETS-2 to its consensus near the transcription start site

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cell Research 2006年第9期16卷 780-796页

作者： Jing De Zhu~(1,2) Qi Fei~(1,*) Peng Wang~(1,*) Fei Lan~(1,*) Da Qin Mao~(1,*) Hong Yu Zhang~1 Xue Biao Yao~3 1 Laboratory of Cancer Eplgenetics and Gene Therapy,The State-Key Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai Jiaotong university,LN 2200/25,Xietu Road,Shanghai 200032,china 2 School of Life sciences,Zhejiang science and technology university,Xiasha university District,Hangzhou,Zhejiang 310018,china, 3 Laboratory of cellular Dynamics,university of science and technology of china,hefei 2.002.,china 2School of Life sciences Zhejiang Science and Technology University Xiasha University District Hangzhou Zhejiang 310018 China 1Laboratory of Cancer Epigenetics and Gene Therapy The State-Key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiaotong University LN 2200/25 Xietu Road Shanghai 200032 China 1Laboratory of Cancer Epigenetics and Gene Therapy The State-Key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiaotong University LN 2200/25 Xietu Road Shanghai 200032 China 1Laboratory of Cancer Epigenetics and Gene Therapy The State-Key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiaotong University LN 2200/25 Xietu Road Shanghai 200032 China 1Laboratory of Cancer Epigenetics and Gene Therapy The State-Key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiaotong University LN 2200/25 Xietu Road Shanghai 200032 China 1Laboratory of Cancer Epigenetics and Gene Therapy The State-Key Laboratory for Oncogenes and Related Genes Shanghai Cancer Institute Shanghai Jiaotong University LN 2200/25 Xietu Road Shanghai 200032 China 3Laboratory of cellular Dynamics University of Science and Technology of China Hefei 230027 China

The hepatocellular carcinoma suppressor 1 (HCCS1) gene was identified by both positional cloning from a predominantregion of loss of heterozygosity (17p13.3) in liver cancer and by functional screening for genes affecting cell proliferationin large-scale transfection *** overexpression results in inhibition of cell proliferation in cell culture and tumorgrowth in nude *** understand its transcription regulation,the promoter architecture has been dissected in *** start of transcription was mapped by primer extension to a C residue,177 nucleotides upstream of the ATG *** assessing the promoter activity of a set of linker-scanning mutants of the minimal promoter (-60 to +148 region) ina transient transfection assay,we found that the+1 to+40 region is critical to HCCSI gene transcription,containingbinding sites for transcription factors NF-κB(-21 to+7 and+40 to+26),p53 (+29 to+9) and ETS (+4 to+20 and+23to+39).Biochemical and molecular analyses revealed that the ETS transcription factors ETS-2 and Elf-1 bind to thetwo ETS sites in situ and contribute significantly to the transcriptionally active state of the HCCS1 gene,while NF-κB,p53 and two other members of the ETS family (ETS-1 and NERF2) appear to play little *** observations provideinsight into the mechanistic aspects of HCCS1 transcription regulation.

关键词： HCCS1 gene transcription regulation ETS p53 NF-κB

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Cu_2ZnSnS_4 thin films prepared by sulfurizing different multilayer metal precursors

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science china(Technological sciences) 2009年第1期52卷 269-272页

作者： ZHANG Jun & SHAO LeXi School of Physical science and technology, Zhanjiang Normal university, Zhanjiang 524048, china School of Physical science and technology Zhanjiang Normal University Zhanjiang 524048 China

Cu2ZnSnS4 (CZTS) thin films were successfully fabricated on glass substrates by sulfurizing Cu-Sn-Zn multilayer precursors, which were deposited by ion beam sputtering and RF magnetron sputtering, respectively. The structural, electrical and optical properties of the prepared films under various processing conditions were investigated in detail. Results showed that the as-deposited CZTS thin films with the precursors by both ion beam sputtering and RF magnetron sputtering have a composition near stoichiometric. The crystallization of the samples, however, has a strong dependence on the atomic percent of constituents of the prepared CZTS films. A single phase stannite-type structure CZTS with a large absorption coefficient of 104/cm in the visible range could be obtained after sulfurization at 520℃ for 2 h. The samples relative to the RF magnetron sputtering showed a low resistivity of 0.073 ?cm and band gap energy of about 1.53 eV. The samples relative to the ion beam sputtering exhibited a resistivity of 0.36 Ωcm and the band gap energy is about 1.51 eV.

关键词： Cu_2ZnSnS_4 solar cell thin film ion beams sputtering RF sputtering

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Synthesis of N^(11)-anchoring biotinylated artemisinin derivatives and their preliminary biological assessment

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science china Chemistry 2010年第1期53卷 119-124页

作者： PAN QiYu1,2,WANG ShaoZhong1,LU JinJian3,MENG LingHua3 & YAO ZhuJun1,2 1Nanjing National Laboratory of Microstructures(NNLM),School of Chemistry and Chemical Engineering,Nanjing university,Nanjing 210093,china 2Joint Laboratory of Green Chemistry,Department of Chemistry,university of science and technology of china,hefei 2.002.,china 3State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Shanghai Institutes for Biological sciences,Chinese Academy of sciences,Shanghai 201203,china. 1. Nanjing National Laboratory of Microstructures (NNLM) School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 China2. Joint Laboratory of Green Chemistry Department of Chemistry University of Science and Technology of China Hefei 230026 China3. State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China

Unique endoperoxide moiety of artemisinin and its derivatives has been considered the functionality exhibiting highly potent antimalarial and anticancer *** investigate the mechanisms of their biological actions,development of suitable molecular probes including biotinylated derivatives is of extreme *** synthesis and preliminary biological assessment of four new biotinylated artemisinin derivatives have been reported in this work.

关键词： artemisinin endoperoxide biotinylated derivative synthesis anticancer activity

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Biological networks to the analysis of microarray data

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Progress in Natural science:Materials International 2006年第12期16卷 1242-1251页

作者： FANG Zhuo~1, LUO Qingming~1**, ZHANG Guoqing~1 and LI Yixue~2**(1. Hubei Bioinformatics and molecular Imaging Key Laboratory,Huazhong university of science and technology, Wuhan 430074, china 2. Shanghai Center for Bioinformation and technology, Shanghai 200235, china) [a]Hubei Bioinformatics and molecular Imaging Key Laboratory Huazhong University of Science and Technology Wuhan China [b]Shanghai Center for Bioinformation and technology Shanghai China

Microarray technology, which permits rapid and large-scale screening for patterns of gene expressions, usually generates a large amount of data. How to mine the biological meanings under these data is one of the main challenges in bioinformatics. Compared to the pure mathematical techniques, those methods incorporated with some prior biological knowledge generally bring better interpretations. Recently, a new analysis, in which the knowledge of biological networks such as metabolic network and protein interaction network is introduced, is widely applied to microarray data analysis. The microarray data analysis based on biological networks contains two main research aspects: identification of active components in biological networks and assessment of gene sets significance. In this paper, we briefly review the progress of these two categories of analyses, especially some representative methods.

关键词： biological networks microarray data analysis subnetwork gene set.

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Functional Analysis of Multiple Transcription Factor Sites in a Regulatory Element of Human ε-Globin Gene

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 673-680页

作者： Chun-Hui HOU, Jian HUANG, and Ruo-Lan QIAN* State Key Laboratory of molecular biology, Institute of Biochemistry and cell biology, Shanghai Institutes for Biological sciences, Chinese Academy of sciences, Graduate School of the Chinese Academy of sciences, Shanghai 200031, china State Key Laboratory of molecular biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

The developmental control of the human ε-globin gene expression is mediated by transcrip- tional regulatory elements in the 5’ flanking DNA of this gene. A previously identified negative regulatory element (–3028 to –2902 bp, termed ε-NRAII) was analyzed and one putative NF-κB site and two GATA sites locate at –3004 bp, –2975 bp and –2948 bp were characterized. Electrophoresis mobility shift assay (EMSA) showed that the putative NF-κB site was specifically bound by nuclear proteins of K562 cells. Data obtained from transient transfection showed that the expression of reporter gene could be upregulated about 50% or 100% respectively when ε-NRAII was inserted upstream of the SV40 promoter or ε-globin gene proximal promoter (–177 bp to +1 bp), suggesting that ε-NRAII might not be a classic silencer. Mutation in the putative NF-κB site or in the GATA site (at –2975 bp) slightly reduced the expression of reporter gene driven by SV40 promoter or ε-globin gene proximal promoter. However, the mutation of GATA site at –2948 bp remarkably reduced the reporter gene activity driven by SV40 promoter, but not by ε-globin gene proximal promoter. Further mutation analysis showed that the negative effect of mutation in GATA site at –2948 bp on SV40 promoter was not affected by the mutation of the putative NF-κB site, whereas it could be abolished by the mutation of GATA site at –2975 bp. Furthermore, the mutation of both GATA sites could synergistically reduce the reporter gene activity driven by ε-globin gene proximal promoter. Those results suggested that ε- NRAII might function differently on the SV40 promoter and ε-globin gene proximal promoter.

关键词： ε-globin gene regulatory element NF-κB GATA

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Microwave peak absorption frequency of liquid

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science china(Physics,Mechanics & Astronomy) 2008年第9期51卷 1254-1263页

作者： HAN GuangZe1 & CHEN MingDong2 1 Department of Physics,South china university of technology,Guangzhou 510640,china 2 Guangzhou Auto College,South china university of technology,Guangzhou 510800,china 1. Department of Physics South China University of Technology Guangzhou 510640 China 2. Guangzhou Auto College South China University of Technology Guangzhou 510800 China

Microwave-assisted extraction is a new effective method which has practical ap-plications in many fields. Microwave heating is one of its physical mechanisms,and it also has the characteristic of selectivity. When the applied microwave fre-quency equals a certain absorption frequency of the material (or specific compo-nent),the material will intensively absorb microwave energy. This is also known as resonant absorption,and the frequency is called the peak absorption frequency which depends on the physical structure of the material. In this work,dynamic hy-drogen bond energy was included in molecular activation energy; with the liquid cell model,the expression of interaction energy between dipolar molecules was derived. The rotational relaxation time was gotten from the Eyring viscosity formula. Then based on the relationship between dielectric dissipation coefficient and re-laxation time,the expression of microwave peak absorption frequency as a func-tion of the material physical structure,rotational inertia and electrical dipole mo-ment of molecules was established. These theoretical formulas were applied to water and benzene,and the calculated results agree fairly well with the experi-mental data. This work can not only deepen the study of the interaction between microwave and material,but also provide a possible guide for the experiment of microwave-assisted extraction.

关键词： microwave-assisted extraction microwave heating relaxation time liquid viscosity peak absorption fre-quency

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Characterization of Function of Three Domains in Dishevelled-1: DEP Domain is Responsible for Membrane Translocation of Dishevelled-1

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cell Research 2004年第4期14卷 324-330页

作者： Wei Jun PAN, Shu Zhao PANG, Tao HUANG, Hui Yun GUO, Dianqing WU, Lin LI Laboratory of molecular cell biology, Institute of Biochemistry and cell biology, Shanghai Institutes for Biological sciences, Chinese Academy of sciences, Graduate School of the Chinese Academy of sciences, 320 Yueyang Rd, Shanghai 200031 College of Life science, Jilin university, Changchun 130061, china. Department of Genetics and Developmental biology, university of Connecticut Health Center, Farmington, Connecticut 06030, USA. 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031. 2College of Life science Jilin University Changchun 130061 China. 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031. 2College of Life science Jilin University Changchun 130061 China. 3Department of Genetics and Developmental biology University of Connecticut Health Center Farmington Connecticut 06030 USA. 1Laboratory of molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031.

Wnt signaling plays an important role in embryogenesis and tumorgenesis. Although the mechanism about how Wnts transduce their signaling from receptor frizzled (Fz) to cytosol has not been understood, dishevelled (Dvl) protein was considered as the intersection of Wnt signal traffic. In this study, we characterized the function of three domains (DIX, PDZ and DEP) of Dvl-1 in canonical Wnt signal transduction and Dvl-1 membrane translocation. It was found both DIX and DEP domain were sufficient to block Wnt-3a-induced LEF-1 transcriptional activity and free cytosol β-catenin accumulation; whereas PDZ domain and a functional mutant form of DEP domain (DEP-KM) had no effect on canonical Wnt signaling. In addition, when cotransfected with Fz-7, DEP domain, but not DIX, PDZ or DEP-KM, translocated and co-localized with Fz-7 to the plasma membrane, which was similar to Dvl-1. Furthermore, it was DEP domain that could block Fz-7-induced membrane translocation of Dvl-1 via a possible competitive mechanism. Thes

关键词： Wnt frizzled-7 dishevelled DIX PDZ DEP membrane translocation signal transduction

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Change in dislocation configuration of deformed Fe-Ni-Nb-Ti-C-B alloy during stress relaxation

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Journal of university of science and technology Beijing 2003年第3期10卷 76-78页

作者： Shaoqiang Yuan, Shanwu Yang, Wenjin Nie, and Xinlai He Department of Materials Physics, university of science and technology Beijing, Beijing 100083, china Tangshan College, Tangshan 063000, china Dept. of Mat. Phys. Univ. of Sci. and Technol. Beijing Beijing 100083 China Tangshan Coll. Tangshan 063000 China

Transmission electron microscopy (TEM) was applied to investigate theevolution of dislocation configuration and strain induced precipitation behavior during relaxationprocess after deformation in Fe-Ni-Nb-Ti-C-B alloy. Experimental results indicate that thedislocation density is very high and distribute randornly before relaxation. As the relaxation timeincreasing, dislocation cells will form gradually by polygonization. The strain inducedprecipitation retards the progress. In the final relaxation stage, most dislocations get rid ofpinning of precipitates and the cells have developed into subgrains with large size.

关键词： relaxation dislocation cell strain induced precipitation

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