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中药与天然药高峰论坛暨第十二届全国中药和天然药物学术研讨会

作者： Chang Chen~1,Xinhua Lu~2,Zhiyong Yan~3,Shaojing Li~1 1 Institute of chinese Materia Medica,china academy of chinese Medical sciences,beijing 100700, china 2 New Drug Research and Development Centre of North china Pharmaceutical Group Corporation, Hebei,050015,china 3 College of life science and engineering,Southwest Jiaotong University,Chengdu 610031,china

P-glycoprotein(P-gp)and glutathione-S-transferase(GST)are key players in multidrug resistance(MDR).Inhibition of the expression of P-gp and/or GST has emerged as a promising therapeutic strategy for the reversal of *** ferulate(CF),isolated from Radix Angelicae sinensis(RAS),strongly inhibited human placental *** 50%inhibition concentration (IC50)was 0.3μM,which is greater than an known GST p1-1 inhibitor,ethacrynic acid(EA), using the established HTS *** analysis and computational docking were used to examine the mechanism of inhibition by *** and the expression of P-gp mRNA were evaluated in the MDR positive B-MD-C1(ADR+/+)cell line to investigate the MDR reversal effect of *** results demonstrated dual inhibition of both P-gp expression and GST activity by CF in a concentration-dependent ***,RAS may be a potential source of MDR reversing agents for pharmaceutical use.

关键词： coniferyl ferulate Radix Angelicae sinensis(Umbelliferae) multidrug resistance glutathione-S-transferase

NTE酯酶活力域真核表达载体的构建及其表达

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毒理学杂志 2005年第3期19卷 169-171页

作者：常平安陈瑞李薇伍一军中国科学院动物研究所分子毒理学实验室

目的构建人神经病变靶标酯酶(NTE)酯酶活力域(NESP)真核表达载体,并在细胞中表达。方法利用含有特定酶切位点的引物,通过PCR扩增出其酯酶活力域,经T载体克隆测序正确后,双酶切回收cDNA片段定向插入到pcDNA3·1(+)中,通过酶切鉴定其真核... 详细信息

目的构建人神经病变靶标酯酶(NTE)酯酶活力域(NESP)真核表达载体,并在细胞中表达。方法利用含有特定酶切位点的引物,通过PCR扩增出其酯酶活力域,经T载体克隆测序正确后,双酶切回收cDNA片段定向插入到pcDNA3·1(+)中,通过酶切鉴定其真核表达载体的构建。然后将其转染到真核细胞中,通过NTE活力测定,检测其表达效果。结果经PCR获得了NTE的1·6kb酯酶活力域的cDNA片段,T载体克隆后测序证实完全正确,然后克隆到真核表达载体中获得了NTE酯酶活力域真核表达载体pcDNA-NEST。瞬时转染到COS7和SH-SY5Y细胞中,NTE的活力与对照细胞相比显著提高。结论成功构建了NEST真核表达载体,并在哺乳动物细胞中表达。

关键词：遗传载体神经病变靶标酯酶酯酶活力域哺乳动物细胞

Identification of Hydroxyl Copper Toxicity to Barley(Hordeum Vulgare) Root Elongation in Solution Culture

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Identification of Hydroxyl Copper Toxicity to Barley(Hordeum...

中国地理学会百年庆典

作者： Wang Xuedong~(1,2),Ma Yibing~2,Hua Luo~1 1 The key Lab of Resource Environment and GIS, College of Resource Environment & Tourism,Capital Normal University,beijing 100037,china 2 Institute of Agricultural Resources and Regional Planning,chinese academy of Agricultural sciences, beijing 100081,china

The effect of pH on the acute toxicity of Cu to barley(Hordeum vulgare ) root elongation was investigated in solution *** results showed that the median effective concentrations(EC50s;i.e.,the concentration that reduced root elongation by 50%based on free Cu activity) were not significantly different in the low-pH range from 4.5 to 6.5,but in the high-pH range from 7.0 to 8.0,a significant effect of pH on EC50s was *** nonlinear relationship between EC50 and H activity in the present study indicated that the increased toxicity with increasing pH in solution may not be caused by decreasing H *** we take account of CuOH activities,a good linear relationship(r>0.97) between the ratio of CuOH activity to free Cu activity and acute Cu toxicity to barley root elongation was achieved,which indicated that the observed toxicity in the high pH range may be caused by CuOH plus free Cu in ***-regression analysis suggested CuOH had a greater binding affinity than Cu at the biotic ligand *** logistic dose-response curve showed that expressing the Cu dose as Cu+ 2.92·CuOH improved the data fit significantly compared to consideration of the free Cu activity ***,our results suggest CuOH was highly toxic to barley root *** enhanced toxicity of CuOH therefore needs to be considered when modeling the effect of pH on Cu toxicity to barley for exposures having pH greater than 6.5.

关键词： Hydroxyl copper pH Biotic ligand model

利用不相容质粒共转化大肠杆菌对Cre重组酶体内重组活性的可视检测

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微生物学报 2005年第1期45卷 125-128页

作者：庞永奇贾洪革方荣祥郭蔼光陈晓英中国科学院微生物研究所植物基因组学国家重点实验室北京100080 西北农林科技大学生命科学学院杨凌712100

源自噬菌体P1的Cre重组酶可以识别 34bp的靶DNA序列loxP ,进行位点特异性的重组反应。为了简便地检测Cre酶在大肠杆菌中的重组活性 ,分别将cre基因和上下游带有loxP的绿色荧光蛋白基因 (gfp)克隆到具有不同抗性的两种不相容质粒中 ,然... 详细信息

源自噬菌体P1的Cre重组酶可以识别 34bp的靶DNA序列loxP ,进行位点特异性的重组反应。为了简便地检测Cre酶在大肠杆菌中的重组活性 ,分别将cre基因和上下游带有loxP的绿色荧光蛋白基因 (gfp)克隆到具有不同抗性的两种不相容质粒中 ,然后将构建的原核表达载体pET30a Cre和pET2 3b loxGFP电击共转化大肠杆菌BL2 1(DE3) ,利用卡那霉素和氨苄青霉素双抗生素抗性进行筛选。通过直接观察转化子的绿色荧光 ,便可以显示Cre酶的体内重组活性 ,并进一步通过SDS PAGE分析、质粒酶切鉴定进行了验证。结果表明 :以gfp为报告基因、通过两种不相容质粒共转化大肠杆菌可以为研究和改进Cre

关键词： Cre重组酶共转化不相容性质粒绿色荧光蛋白

Catalyst activity Prediction of Asymmetric Complexes by Modified MANCC Method

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Catalyst Activity Prediction of Asymmetric Complexes by Modi...

2007年全国高分子学术论文报告会

作者： Xiongfei Zhang Shaw Ling Hsu Xiaozhen Yang 1.Department of Polymer Science and Engineering,University of Massachusetts Amherst 120 Governors Drive,Amherst,MA 01003,USA 2.State key laboratory of Polymer Physics and Chemistry and Center for Molecular sciences,Institute of Chemistry,The chinese academy of Science,beijing 100080,china

＜正＞Polyolefins are among the most important forms of commercial plastics due to their low cost and versatile *** discovery of catalysts for the synthesis of such materials remained a challenging *** the catalysts e... 详细信息

关键词： catalyst MANCC efficient charge activity

Impaired response inhibition in chronic heroin users

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Impaired response inhibition in chronic heroin users

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Yi YUAN~(1,2),Zu-De ZHU~3,Zi-Rui HUANG~1,Zhi-Ling ZOU~4,Jin-Fu SHI~1,Xu-Chu WENG~(1,*) 1 laboratory for Higher Brain Function,Institute of Psychology,The chinese academy of sciences,beijing 100101, china 2 Department of Psychology,Tejing College,beijing 100621,china 3 Department of Psychology,South china Normal University,Guangzhou,china 4 Department of Psychology,South-West china Normal University,Chongqing,china

＜正＞Objective To address whether and how the inhibition function was impaired in heroin users,brain activity in heroin users and healthy subjects was examined while they performed a stop-signal task（SST） using event-related fMRI. The SST is believed to an ideal task for revealing response inhibition and its neural *** Twelve right-handed heroin users and 12 right handed healthy control subjects,matched in terms of sex,age,education years,were *** functional MRI data collecting,the subjects performed the SST,which consisted of Go trials and Stop *** the Go trials,the subjects were asked to click the left and right button of mouse to indicate the orientation of the *** the Stop trials,stimuli were followed by a cross（stop signal） with various delays,which cued the subjects to cancel the *** data were analyzed by AFNI software *** The successful stop trial was the key to reveal neural activity associated with response *** with the healthy subjects,the task elicited weaker activation in the frontal-temporal-parietal-occipital network as well as cingulate cortex and cerebellum,while activation in the right superior frontal gyrus and parahippocampal gyrus was stronger in heroin *** Response inhibition was impaired in heroin users along with abnormal activity in a distributed network involving cortical and subcortical structures.

关键词： fMRI stop signal heroin impulsivity response inhibition

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太阳大气磁精细结构的射电探讨

天文学进展 1997年第3期15卷 198-217页

作者：傅其骏中国科学院北京天文台北京100080

太阳大气磁场的研究对于太阳大气物理及太阳活动研究是十分重要的。目前探测光球以外的日冕、色球、过渡区磁场的几乎唯一办法，是在紧密联系其他频谱段取得的信息基础上使用射电观测。根据在微波、米波段有关辐射机制和传播过程，介绍... 详细信息

太阳大气磁场的研究对于太阳大气物理及太阳活动研究是十分重要的。目前探测光球以外的日冕、色球、过渡区磁场的几乎唯一办法，是在紧密联系其他频谱段取得的信息基础上使用射电观测。根据在微波、米波段有关辐射机制和传播过程，介绍了推导磁场讯息的基本射电方法。在按高度分布的磁场上，还存在磁场的精细结构，这被认为是与裂化的场耗散（重连）紧密相关，并产生以时电辐射显现出来的粒子加速及等离子体加热。微波爆发中快速精细结构的动态频谱为精细磁结构的诊断提供了一种重要的手段，这也是我国正在建立中的作为太阳活动第23周极大期重点项目的“太阳射电宽带频谱仪”的主要科学目标。

关键词：太阳日冕大气物理磁场耀斑太阳射电

Effects of Ultra-drying on Vigor and Physiological characteristics of Hippophae rhamnoides Seeds

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chinese Forestry Science and Technology 2010年第1期9卷 53-59页

作者： LIU Yan1 LIU Guangquan1, 2, 3 LI Qingmei4 DUAN Xinfang5 HOU Longyu1 1. International Research and Training Center on Erosion and Sedimentation, beijing 100048, P.R. china 2. china Institute of Water Resources and Hydropower Research, beijing 100048, P.R. china 3. Northwest Agricultural and Forestry University, Yangling 712100, P.R. china 4. Research Institute of Forestry, chinese academy of Forestry, beijing 100091, P.R. china 5. Institute of New Forestry Technology, chinese academy of Forestry, beijing 100091, P.R. china

Studies of ultra-drying and accelerated aging on Hippophae rhamnoides seeds from the arid and semiarid areas of northwest china were conducted. The results showed that H. rhamnoides seeds had higher desiccation endurance. When seeds were desiccated to 26 g/kg, their germination percentage, vigor indices and enzyme activity could be retained. After 30-day accelerated aging, ultra-dried seeds had better anti-aging ability and storability compared to natural dried seeds, and the best choice for the MC of seed was 34 g/kg. Accordingly, ultra-drying treatment could improve the storability.

关键词： ultra-drying accelerated aging seed vigor enzyme activity Hippophae rhamnoides

SCIRRIO exerts its neurotrophic activity through thyrotropin releasing hormone receptor 2

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SCIRRIO exerts its neurotrophic activity through thyrotropin...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Yong LIU~1,Xiao-Yan SHI~1,Hai-Ping QUE~1,Yu WANG~(1,3),Yan-Li NI~1,Zhen-Lian MA~1,Xin LI~1,Tao LIU~1,Zhi-Yi ZHANG~4,Shu-Qian JING~(1,2),Shao-Jun LIU~(1,*) 1 Department of Neurobiology,Institute of Basic Medical sciences,academy of Military Medical sciences,27 Taiping Road,beijing 100850,china 2 Department of Clinical Immunology,Amgen Inc.,Thousand Oaks,CA,USA 3 State key laboratory of Polymer Physics and Chemistry,Changchun Institute of Applied Chemistry,chinese academy of sciences,5625 Renmin Road,Changchun 130022,china 4 chinese national Center of Biomedical Analysis,27 Taiping Road,beijing 100850,china

＜正＞Spinal cord injury and regeneration related gene No 10（SCIRR10）was first identified as an EST in our efforts of seeking genes responsive to spinal cord neuron *** levels of SCIRRIO mRNA expression were detected in adult CNS and several other *** injured spinal cord,the level of SCIRRIO expression was temporally elevated.A His-tagged-SCIRR10 affinity column was able to capture thyrotropin releasing hormone receptor 2（TRHR2）from spinal cord lysates,suggesting that SCIRR10 interacted with *** interaction between SCIRR10 and TRHR2 was further confirmed by the co-localization of a GFP-SCIRR10 fusion protein with RFP-TRHR2 fusion protein expressed on COS7 *** PC12 cells,recombinant SCIRR10 and conditional medium containing SCIRR10 induced activation of the phospholipase C and MAPK pathways,the major signal transduction pathways shared by *** SCIRR10 protein and conditional medium containing SCIRR10 also enhanced the sprouting and growth of neurites from cultured embryonic rat cortex *** biological function to promote neurite growth could be interdicted with pertussis *** data demonstrated that SCIRR10 is a novel neurotrophic factor that may play an important role in the regeneration of CNS injury.

关键词： SCIRR10 TRHR2 neural regeneration

In situ localization and substrate specificity of earthworm protease-ii and protease-iii-1 from Eisenia fetida

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In situ localization and substrate specificity of earthworm ...

第一届全国脑与认知科学学术研讨会

作者： Jing Zhao Rong Xiao Rons Pan Jian He Xiang Liu Rong Fan Cheng Wu Ying Liu Xiang Liu Rong-Qiao He State key laboratory of Brain and Cognitive Science Institute of Biophysics Graduate School Baiao Pharmaceuticals beijing C.L. The chinese academy of sciences 15 Da Tun Road Chao Yang District beijing 100101 china.

＜正＞Recently, earthworm proteases have been studied as agents to function in fibrinolysis. In our experiments, earthworm protease-Ⅱ（EfP-Ⅱ） and -Ⅲ-1 （EfP-Ⅲ-1） were isolated and purified from Eisenia fetida. As shown by fibrin plate assay of the sections of earthworm, the enzymic activity was mainly detected around the clitellum, but not in the head region and the posterior intestines. In the presence of anti-EfP-Ⅱor anti-EfP-Ⅲ-1 sera, the immunological signals of the two isozymes were markedly detected in the anterior alimentary wall by immunohistochemistry, suggesting that EfP-Ⅱand EfP-Ⅲ-1 were localized and expressed in

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