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第五届全国化学生物学学术会议

作者： Lihua Jiang~1 Zhen Xue~2 Bihua Yao~2 Weizhi Chen~(3*) Rihe Liu~4 1 State key laboratory of Phytochemistry and Plant Resources in West china,Kunming Institute of Botany,chinese academy of sciences,Kunming 650204,china 2 beijing Kaizheng Biotech Development Co.Ltd.,beijing 100850,china 3 SinoGenoMax Co.Ltd.,chinese Human Genome Center,beijing 100176,china 4 School of Pharmacy,University of North Carolina at Chapel Hill,Chapel Hill,NC27599,USA.

＜正＞In an in vitro screening of a crude extract library of chinese mountain herbs for protease inhibitors,six active species from Rhodiola（Crassulaceae）were identified to specifically inhibit 20S *** contrast,these crude Rhodiola extracts inhibits Calpain 1 and 2,cathepsin B,and caspases at much higher *** molecular weight fractions from the extract of the most potent species,Rhodiola crenulata,remained 20s proteasme inhibition *** fraction

关键词： 20s proteasome Rhodiola crenulata tumor IκB

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The Effect of Accelerated Aging on the Energy Supply of Maize Seed

The Effect of Accelerated Aging on the Energy Supply of Maiz...

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第二届国际整合植物生物学学术研讨会

作者： Ze-Lin Wang,Chao Song,Yue Zhao,Xia Xin and Xin-Xiong Lu~* Institute of Crop Science,chinese academy of Agricultural sciences,beijing 100081,china

It is necessary to mobilize storage compounds to provide energy for seed *** starchy seeds,such as maize seeds,the energy for germination is mainly provided by *** proteomic analyses showed that in maize seed,the expression of some proteins responsible for energy supply,such as 3-phosphoglycerate kinase,malate dehydrogenase and ATP synthase, were significantly down-regulated by accelerated *** was presumed that the energy supply system was closely related to seed *** further study the effect of accelerated aging on energy supply system at the level of the catalytic activity of the enzyme and gene expression,we detected the activity of key enzymes of glycolysis and phosphate pentose pathway by spectrophotography,such as hexokinase,glucose-6-phosphate dehydrogenase and pyruvate ***,the expression of their related genes was determined by RT-PCR and the level of ATP was estimated by bioluminescence,so as to explore the effect of accelerating aging on the pathway and the efficiency of energy *** the present study,maize(Zea mays ***. Jiyu 8) seeds,with the initial germination of 96%,were accelerated aged at 50℃,and seeds with germination percentages of 85%,73%,54%,22%,0%were used for further *** results showed that with declining germination,the activities of the three enzymes and gene expression displayed a downward trend,and ATP content decreased in non-imbibing seeds after *** the imbibition process,the activity of hexokinase gradually increased in non-aging seeds,but this trend was inhibited in aging *** the seeds with germination percentage higher than 54%, the activity of phosphate-6-glucose dehydrogenase slightly decreased until 12 h imbibition,and then increased to 1.5-1.8 times that of dry seeds after 24 h of imbibition,whereas no obvious changes in seeds with germination lower than 22%were *** activity of pyruvate kinase in non-aging dry seeds was 1.16 umol NADH mg protein min,but

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A New Analytical Method of the Water Resources Allocation in china

A New Analytical Method of the Water Resources Allocation in...

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2014年环境污染与大众健康学术会议

作者： Yanqi ZHAO1 2 3 Changming LIU1 Ying YANG2 Hongxing ZHENG1 1Institute of Geographic sciences and Natural Resources Research CAS beijing 100101 china 2Institute of Resources & Environment Henan Polytechnic University(HPU) Jiaozuo Henan 45400 china 3Graduate University of chinese academy of sciences beijing 100101 china

The paper introduces the Gini coefficient of economics as a new method of analyzing the inter-provincial allocation of water resources in *** calculated the Gini coefficient for selected indices based upon the statistical data for each province from 1997 to 2008,respectively taking the population,GDP and the cultivated area as the basic matching ***,we proposed two novel indexes:the Dependence Coefficient to weigh the degree of reliance on the surface water or groundwater for the separated regions and the Burden Coefficient of water use to judge the efficiency and the level of fairness of water consumption by different *** results show that the allocation of water resources calculated per capita,per unit GDP and per unit area of arable land is very *** particular,some provinces located north of the Yangtze River lack water resources and strongly depend on the *** water efficiency is there lower than in other parts of ***,in order to alleviate the water stress in the northern provinces of the country,an improvement of the technological level of water resources exploitation and utilization and also the restructuring of the economic activity are necessary.

关键词： water resources allocation Gini coefficient Dependence Coefficient Burden Coefficient

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Nitrous Oxide Catalytic Decomposition Technology for Adipic Acid Production

Nitrous Oxide Catalytic Decomposition Technology for Adipic ...

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第五届全国环境催化与环境材料学术会议

作者： Lan Dong Li, Qun Shen, Jun Jie Yu, Zheng Ping Hao Research Center for Eco-Environmental sciences, chinese academy of sciences, beijing 100085, P. R china.

Nitrous oxide （N2O） had been long considered as a relative harmless gas and not received much attention from an environmental point of view. However, during the last decades, N2O is recognized to be harmful to our environment, since it greatly contributes to the greenhouse effect as well as the destruction of ozone in the stratosphere 1. N2O has a very long atmospheric lifetime of about 120 years and the global warming potential （GWP） for 100-year time horizon per N2O molecule is about 310 times greater than that of carbon dioxide （CO2） 2. As reported by IPCC, anthropogenic N2O emissions make a contribution of about 6 % to the global greenhouse effect and 6 % to the ozone layer depletion 3. The two major sources of anthropogenic N2O emissions from industry are the production of adipic acid and nitric acid. Therefore, a great effort is being made to reduce N2O emission from the above two sources. Heterogeneous catalytic decomposition of N2O （2N2O → 2N2 + O2） is proposed as the most economical method for the removal of N2O emissions from adipic acid production, with very high N2O concentration. As for the catalytic decomposition of N2O, a key problem is that catalysts with sufficient activity and durability have to be developed. In the present work, a type of monolithic Fe-zeolite/cordierite is developed as promising catalyst for N2O decomposition for adipic acid production. Zeolite/cordierite sample was prepared by in situ hydrothermal method as described in literature 4, and iron was introduced into zeolite/cordierite by conventional wet ion exchange method. The as-prepared Fe-zeolite/cordierite is very active for the catalytic decomposition of N2O and a 100 % N2O conversion （to N2 and O2） can be obtained at the above 723 K. The durability of Fe-zeolite/cordierite catalyst is tested under simulated condition of typical adipic acid plant. Time-on-stream behavior of Fe-zeolite/cordierite suggests the remarkable durability of catalyst, and no obvious activity lost can

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Enhanced Sensitivity to Oxidative Stress in Transgenic Tobacco Plants with Decreased Glutathione Reductase activity Leads to a Decrease in Ascorbate Pool and Ascorbate Redox State

Enhanced Sensitivity to Oxidative Stress in Transgenic Tobac...

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中国遗传学会植物遗传和基因组学专业委员会2009年学术研讨会

作者： Shunhua Ding,Qingtao Lu,Yan Zhang,Zhipan Yang,Xiaogang Wen,Lixin Zhang,and Congming Lu~* Photosynthesis Research Center,key laboratory of Photosynthesis and Environmental Molecular Physiology,Institute of Botany,chinese academy of sciences,beijing,100093

To investigate the possible mechanisms of glutathione reductase(GR) in protecting against oxidative stress,we obtained transgenic tobacco(Nicotiana tabacum) plants with 30-70%decreased GR activity by using a gene enco... 详细信息

关键词： Glutathione reductase oxidative stress transformed tobacco

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SO2-promoted V2O5/CNT catalyst for NO reduction with ammonia at low temperatures

SO2-promoted V2O5/CNT catalyst for NO reduction with ammonia...

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第五届全国环境催化与环境材料学术会议

作者： Shuli Bai 1,2, Jianghong Zhao1 , Jianfeng Zheng1,2, Chang Song1,2, Zhenping Zhu1 (1 State key laboratory of Coal Conversion, Institute of Coal Chemistry, CAS, Taiyuan 030001, china 2 Graduate School of the chinese academy of sciences, beijing 100039, P R china)

SO2-promoted carbon nanotubes-supported vanadium oxide catalyst was studied for SCR of NO with NH3 at low temperatures (<250 oC). The results show that the V2O5/CNT catalysts have a high activity for the NO reduction ... 详细信息

SO2-promoted carbon nanotubes-supported vanadium oxide catalyst was studied for SCR of NO with NH3 at low temperatures (activity for the NO reduction with NH3 in a low-temperature range of 200-250 oC and exhibit a significant activity improvement with increasing V2O5 loading within a studied range of 0.1 wt%. The catalysts show very high stability in the presence of SO2, more interestingly, their activities are significantly promoted other than poisoned by SO2.

关键词： Carbon nanotube vanadium oxide NO reduction Ammonia SO2 promotion

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CpTI蛋白在大肠杆菌中的高效融合表达及其纯化与活性测定

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生物工程学报 2003年第1期19卷 63-68页

作者：杨丽琛常团结陈宛新杨晓光朱祯1 中国疾病预防控制中心营养与食品安全所卫生部微量元素营养重点实验室北京100050 中国科学院遗传研究所植物遗传分子操作开放实验室北京100101

豇豆胰蛋白酶抑制剂 (CowpeaTrypsinInhibitor)基因 (CpTI)因其抗虫谱广及不易耐受等优点在植物基因工程中得到广泛应用。为进行遗传修饰食品 (Geneticallymodifiedfoods,GMF)中所含CpTI基因表达产物的安全性评价 ,我们需要在体外微生... 详细信息

豇豆胰蛋白酶抑制剂 (CowpeaTrypsinInhibitor)基因 (CpTI)因其抗虫谱广及不易耐受等优点在植物基因工程中得到广泛应用。为进行遗传修饰食品 (Geneticallymodifiedfoods,GMF)中所含CpTI基因表达产物的安全性评价 ,我们需要在体外微生物体系中获得大量的CpTI蛋白。采用pGEX融合蛋白表达系统 ,将CpTI与GST的编码序列在大肠杆菌BL2 1中进行融合表达 ,表达产物达到菌体总蛋白的 4 0 %。经一步GlutathioneSephrose 4B亲和纯化 ,融合蛋白GST CpTI纯度达到 90 %以上。将Thrombin蛋白酶与融合蛋白过夜作用 ,可获得切掉了GST标签的CpTI蛋白。活性测定显示GST CpTI及CpTI蛋白均具有明显的胰蛋白酶抑制剂活性。用纯化的融合蛋白免疫家兔还可诱导产生高效价的抗体 ,经ELISA检测抗体滴度 >1∶2 0 0 0 0。WesternBlotting实验显示 ,无论是菌体超声裂解后总蛋白中的CpTI蛋白或是经纯化后的CpTI蛋白均可与自制的抗体发生特异性结合。这为进一步进行CpTI蛋白的安全性评价工作奠定了良好基础。

关键词： CpTI蛋白大肠杆菌高效融合表达纯化活性测定豇豆胰蛋白酶抑制剂遗传修饰食品安全性评价抗虫蛋白

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Voltage-sensitive dye imaging of dysgranular cortex activation evoked by ipsilateral whisker stimulation

Voltage-sensitive dye imaging of dysgranular cortex activati...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Zhi-Jie WANG~1,Xin GAO~1,Bing LI~(1,*),Jian-Young WU~(2,*) 1 State key laboratory of Brain and Cognitive Science,Institute of Biophysics,chinese academy of sciences,beijing 100101,china 2 Department of Physiology and Biophysics,Georgetown University,Washington,DC 20057,USA

＜正＞Integration of bilateral sensory signals is important for the survival of an *** primary somatosensory cortex（SI） can be activated by both contralateral and ipsilateral whisker stimulation,though it does not receive directly ipsilateral sensory *** has been proposed that the activity evoked by ipsilateral whisker stimulation is transmitted from contralateral SI,but the pathway for the transmission remains *** use voltage-sensitive dye imaging to visualize the whisker-evoked responses in rat somatosensory cortex and surrounding *** find that ipsilateral whisker stimulation activates first the dysgranular zones（DZ） outside the barrel cortex,and the evoked activity forms a propagating wave that spreads into the whisker sub-barrel *** stimulation to the contralateral SI further confirms the *** initiation sites in the DZ activation are close to each other when different ipsilateral whiskers are stimulated.A comparison between the responses to contralateral and bilateral stimulation indicates that the involvement of DZ activation may improve the discriminability of the *** results suggest that ipsilateral whisker stimulation first initiates activation in the corresponding barrel in the contralateral side,and activates the ipsilateral DZ via the interhemispheric connections between the DZ *** ipsilateral somatosensory activation has a non-specific representation in the DZ and does not overlap with that of contralateral whisker *** activation may participate in the sensory processing,gain control,and bilateral sensory integration.

关键词： dysgranular zones primary somatosensory cortex interhemispheric connections ispilateral whisker stimulation voltage-sensitive dye imaging

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对地日冕物质抛射研究

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天文学进展 2002年第1期20卷 33-44页

作者：宋丽敏张军杨志良汪毓明汪景琇中国科学院国家天文台北京100012 北京师范大学天文系北京100875 中国科学技术大学地球和空间科学系合肥230026

日冕物质抛射，作为太阳大气中频繁发生的极为壮观的活动现象，越来越受到太阳物理学家的关注。其中一类特殊的抛射事件──对地日冕物质抛射，通常与大的地磁暴、行星际激波和高能粒子事件相伴生，具有强烈的地球物理效应。是影响空间... 详细信息

日冕物质抛射，作为太阳大气中频繁发生的极为壮观的活动现象，越来越受到太阳物理学家的关注。其中一类特殊的抛射事件──对地日冕物质抛射，通常与大的地磁暴、行星际激波和高能粒子事件相伴生，具有强烈的地球物理效应。是影响空间天气的主要因素之一。概括了对地日冕物质抛射的研究现状，重点介绍了与对地日冕物质抛射事件相联系的光球向量磁场演化的观测研究成果，并由典型事件探讨了暗条爆发、耀斑等剧烈太阳活动和对地日冕物质抛射之间的密切关系，提出了尚待解决的主要问题和进一步的研究方向。

关键词：对地日冕物质抛射太阳活动重磁联太阳大气地磁暴耀斑

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Long-term protection effects of national reserve to forest vegetation in 4 decades: biodiversity change analysis of major forest types in Changbai Mountain Nature Reserve, china

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Science china(Life sciences) 2008年第10期51卷 948-958页

作者： BAI Fan1, SANG WeiGuo1, LI GuangQi1, LIU RuiGang1, CHEN LingZhi1 & WANG Kun2 1 State key laboratory of Vegetation and Environment Change, Institute of Botany, chinese academy of sciences, beijing 100093, china 2 Nature Reserve Bureau of Changbaishan, Jilin 210011, china State key laboratory of Vegetation and Environment Change Institute of Botany Chinese Academy of Sciences Beijing 100093 China

The Changbai Mountain Nature Reserve (CNR) was established in 1960 to protect the virgin Korean pine mixed hardwood forest, a typical temperate forest of northeast china. We conducted systematic stud- ies of vascular diversity patterns on the north slope of the CNR mountainside forests (800-1700 m a.s.l.) in 1963 and 2006 respectively. The aim of this comparison is to assess the long-term effects of the protection on plant biodiversity of CNR during the interval 43 years. The research was carried out in three types of forests: mixed coniferous and broad-leaved forest (MCBF), mixed coniferous forest (MCF), and sub-alpine coniferous forest (SCF), characterized by different dominant species. The alpha diversity indicted by species richness and the Shannon-Wiener index were found different in the same elevations and forest types during the 43-year interval. The floral composition and the diversity of vascular species were generally similar along altitudinal gradients before and after the 43-year interval, but some substantial changes were evident with the altitude gradient. In the tree layers, the dominant species in 2006 were similar to those of 1963, though diversity declined with altitude. The indices in the three forest types did not differ significantly between 1963 and 2006, and these values even increased in the MCBF and MCF from 1963 to 2006. However, originally dominant species, P. koraiensis for ex- ample, tended to decline, while the proportion of broad-leaved trees increased, and the species turn- over in the succession layers trended to shift to higher altitudes. The diversity pattern of the under canopy fluctuated along the altitudinal gradient due to micro-environmental variations. Comparison of the alpha diversity in the three forests shows that the diversity of the shrub and herb layer decreased with time. During the process of survey, we also found some rare and medicinal species disappeared. Analysis indicates that the changes of the diversity pattern

关键词： plant species diversity climate change forest community human activity Changbai Mountain

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