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Calcitonin gene-related peptide inhibits interleukin-1β-induced interleukin-8 secretion in human type Ⅱ alveolar epithelial cells

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Acta Pharmacologica Sinica 2006年第10期27卷 1340-1345页

作者： Wen-jing LI Teng-ke WANG Xian WANG~2 Department of Physiology,School of Basic Medical sciences,Peking University,key laboratory of molecular cardiovascular science,ministry of education,beijing 1.0083,china Department of Physiology School of Basic Medical Sciences Peking University Key Laboratory of Molecular Cardiovascular Science Ministry of Education Beijing 100083 China

Aim:Our previous data have shown that type Ⅱ alveolar epithelial(AEⅡ)cellsexpress neuropeptide calcitonin gene-related peptide(CGRP),and that pro-in-flammatory factor interleukin 1-β(IL-1β)induces CGRP secretion in the A549 hu-man AEⅡ cell *** the present study,we investigated the effect of endogenousand exogenous CGRP on IL-1β-induced chemokine interleukin-8(IL-8)***:We used enzyme-linked immunosorbent assay(ELISA)and RT-PCR todetect IL-8 protein and mRNA levels,respectively,siRNA and the stably trans-fected cell line were used to knock down and overexpress the CGRP gene,respectively,and chemiluminescence assay was used to detect reactive oxygenspecies(ROS)***:CGRP-1 receptor antagonist hCGRP8-37(0.1-1nmol·L-1)greatly amplified IL-1β-induced IL-8 *** inhibition of CGRPexpression by siRNA significantly increased IL-8 secretion upon IL-1β ***,cell clones stably transfected with CGRP showed significantly inhibitedmRNA and protein levels of IL-8 induced by 1L-1β.Conclusion:These data implythat AEⅡ cell-derived CGRP suppress IL-1β-induced IL-8 secretion in an autocrine/paracrine *** investigation showed that CGRP attenuated IL-1β-arousedROS formation,which is an early indication of pro-inflammatory factor signaling.

关键词： type Ⅱ alveolar epithelial cells interleukinl interleukin-8 calcitonin gene-related peptide anti-inflammation lung reactive oxygen species

Cloning and characterization of cotton heteromeric acetyl-CoA carboxylase genes

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Progress in Natural science:Materials International 2007年第12期17卷 1412-1418页

作者： Qiao Zhixin and Liu Jinyuan(laboratory of molecular Biology and Protein science laboratory of the ministry of education, Department of Biological sciences and Biotechnology, Tsinghua University, beijing 100084, china) laboratory of molecular Biology and Protein science laboratory of the ministry of education Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Heteromeric acetyl-coenzyme A (CoA) carboxylase (ACCase) catalyzes the formation of malonyl-CoA from acetyl-CoA. It plays an essential role in fatty acid synthesis in prokaryotes and most of plants. The heteromeric ACCase is composed of four subunits: biotin carboxylase (BC), biotin carboxyl carrier protein (BCCP), and α- and β-subunits of carboxyltransferase (α- and β-CT). In this study, we cloned five novel genes encoding the subunits of heteromeric ACCase (one BC, BCCP and β-CT, and two α-CTs) from cotton (Gossypium hirsutum cv. zhongmian 35) by RACE-PCR. The deduced amino acid sequence of these cDNAs shares high similarity with other reported heteromeric ACCase subunits. The phylogenetic analysis indicated that the different subunits of heteromeric ACCase were grouped in a similar pattern. Southern blot analysis revealed the milti-copy patterns of these heteromeric ACCase genes in cotton genome. Semi-quantitative RT-PCR demonstrated that heteromeric ACCase genes were constitutively expressed in all of the cotton tissues, but the transcripts accumulated at a relatively low level in roots. To our knowledge, this is the first report about characterization of the heteromeric ACCase genes in cotton.

关键词： heteromeric ACCase cotton gene cloning fatty acid synthesis.

Hydrogen sulfide ameliorates vascular calcification induced by vitamin D3 plus nicotine in rats

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Acta Pharmacologica Sinica 2006年第3期27卷 299-306页

作者： Sheng-ying WU~(2,6) Chun-shui PAN~3 Bin GENG~3 Jing ZHAO~3 Fang YU~3 Yong-zheng PANG~3 Chao-shu TANG~4 Yong-fen QI~(3,4,5) ~2Institute of cardiovascular Disease,Peking University First Hospital.beijing 100034 ~3Department of Physiology and Pathophysiology,Peking University Health science Center,beijing 1.0083,china,~4key laboratory of molecular cardiovascular science,ministry of education,beijing 1.0083,china 2Institute of cardiovascular Disease Peking University First Hospital Beijing 100034 China 3Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 3Department of Physiology and Pathophysiology Peking University Health Science Center Beijing 100083 China 4key laboratory of molecular cardiovascular science Ministry of Education Beijing 100083 China 4key laboratory of molecular cardiovascular science Ministry of Education Beijing 100083 China

Aim:To investigate the role of the endogenous cystathionine γ-synthase(CSE)/hydrogen sulfide(H2S)pathway in vascular calcification in ***:A ratvascular calcification model was established by administration of vitamin D3 plusnicotine(VDN).The amount of CSE and osteopontin(OPN)mRNA was deter-mined by using semi-quantitative reverse-transcription polymerase chain *** calcium content,45Ca2+accumulation and alkaline phosphatase(ALP)activitywere measured.H,S production and CSE activity were ***:yonKossa staining produced strong positive black/brown staining in areas amongthe elastic fibers of the medial layer in the calcified *** calcium content,45Ca2+accumulation and ALP activity in calcified arteries increased by 6.77-,1.42-,and 1.87-fold,respectively,compared with *** expression of the OPNgene was upregulated(P34.8%,40.75% and 63.5% lowerin the low-dosage NariS group than in the VDN group,respectively(P3.9%,37.8 % and 46.2% lower in the aorta in the high-dosage NariS group than in theVDN group,respectively(P<0.01).The expression of the OPN gene ***:The production of H,S,and CSE activity were de-creased and CSE gene expression was downregulated in rats with vascularcalcification.H2S can ameliorate vascular calcification,suggesting that the H2S/CSE pathway plays a regulatory role in the pathogenesis of vascular calcification.

关键词： hydrogen sulfide cystathionine-lyase vascular calcification mRNA

molecular dynamics simulations of non-Fourier heat conduction

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Progress in Natural science:Materials International 2008年第8期18卷 999-1007页

作者： Qixin Liu, Peixue Jiang *, Heng Xiang key laboratory for Thermal science and Power Engineering of ministry of education, Department of Thermal Engineering, Tsinghua University, beijing 100084, china key laboratory for Thermal science and Power Engineering of ministry of education Department of Thermal Engineering Tsinghua University Beijing 100084 China

Unsteady heat conduction is known to deviate significantly from Fourier’s law when the system time and length scales are within certain temporal and spatial windows of relaxation. Classical molecular dynamics simulations were used to investigate unsteady heat conduction in argon thin films with a sudden temperature increase or heat flux at one surface to study the non-Fourier heat conduction effects in argon thin films. The studies were conducted with both pure argon films and films with vacancy defects. The temperature profiles in the argon films showed the existence of mechanical waves when the thin film was suddenly heated and the wave nature of the heat propagation. The flux phase relaxation time, sq, and the temperature phase relaxation time, st, were calculated from the temporal variations of the energy flux and temperature distribution in the film. Comparisons of the MD temperature profiles with temperature profiles predicted by Fourier’s law show that Fourier’s law is not able to predict the temperature variations with time. Different film thicknesses were also studied to illustrate the variation of the time needed for the films to reach steady-state temperature profiles after a sudden temperature rise at one surface and to illustrate the finite speed of the energy waves.

关键词： molecular dynamics simulation Non-Fourier heat conduction Argon thin film

Distinct actions of intermittent and sustained β-adrenoceptor stimulation on cardiac remodeling

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science china(Life sciences) 2011年第6期54卷 493-501页

作者： MA XiaoWei1,3,SONG Yao1,3,CHEN Chao1,3,FU YongNan1,3,SHEN Qiang2,3,LI ZiJian1,3 & ZHANG YouYi1,3 1Institute of Vascular Medicine,Peking University Third Hospital,beijing 100191,china 2Institute of cardiovascular science,Peking University,beijing 100191,china 3key laboratory of molecular cardiovascular sciences,ministry of education,beijing 100191,china 1. Institute of Vascular Medicine Peking University Third Hospital Beijing 100191 China3. Key Laboratory of Molecular Cardiovascular Sciences Ministry of Education Beijing 100191 China2. Institute of Cardiovascular Science Peking University Beijing 100191 China

Heart disease is associated with increased sympathetic nerve activity and elevated levels of circulating catecholamines,resulting in chronic stimulation of the β-adrenergic receptors (β-AR) and consequent pathological cardiac ***,chronic administration of the β-AR agonist isoproterenol (ISO) has been most commonly used to model β-AR-induced cardiac ***,it remains unclear whether β-AR-mediated cardiac remodeling and dysfunction differs between sustained versus pulsatile (intermittent) exposure to a β-***,we compare the effects of intermittent versus sustained administration of ISO on cardiac remodeling and function in *** were administered 5 mg (kg d)-1 ISO for 2 weeks either by daily subcutaneous injection,or continuous infusion via an implanted osmotic *** function and remodeling were determined by echocardiography,micromanometry and ***,Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) were utilized to define the proteins and genes *** sustained and intermittent administration of ISO resulted in a similar degree of cardiac hypertrophy (16% and 19%,respectively).However,mice receiving ISO by daily injection developed more severe ventricular systolic and diastolic dysfunction and myocardial fibrosis compared with mice receiving ISO via the osmotic *** disparity in results between the delivery methods is suggested to be due,at least in part,to increased expression of fibrogenic factors,including connective tissue growth factor (CTGF) and NADPH oxidase (NOX4),in mice receiving intermittent application of *** summary,compared with sustained exposure to a β-AR agonist,intermittent β-AR stimulation leads to more severe cardiac dysfunction and *** findings not only further our understanding of β-AR function in the setting of cardiac pathophysiology,but also highlight that significant differences can result dependent upon the mod

关键词： β-adrenoceptor reactive oxygen species NADPH oxidase cardiac hypertrophy cardiac fibrosis

维普期刊数据库

Reinvestigation on the Reaction of Salicylic Chloride with Salicylic Amide and X-ray Crystal Structure of a New Macrocyclic Polyester

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Chinese Journal of Structural Chemistry 2003年第5期22卷 512-516页

作者： DU Da-Ming② CHEN Xiao HUA Wen-Ting JIN Xiang-Lin (key laboratory of Bioorganic Chemistry and molecular Engineering of ministry of education, College of Chemistry and molecular Engineering, Peking University, beijing 100871, china) key laboratory of Bioorganic Chemistry and molecular Engineering of ministry of education College of Chemistry and Molecular Engineering Peking University

The reaction of salicylic chloride and salicylic amide at 170 ℃ gave benzoxazine and a new macrocyclic polyester 3, whose crystal structure has been determined by single-crystal X-ray diffraction analysis. Compound 3: C28H16O8, Mr = 480.41, monoclinic, space group P21/n, a = 15.353(3), b = 14.196(3), c = 21.147(4) ? b = 103.32(3), V = 4485(2) ?, Z = 8, F(000) = 1984, Dc = 1.423 g/cm3 and m = 0.106 mm-1. The final R = 0.0535 and wR = 0.1275 for 7903 observed reflections (I ≥ 2(I)). In the asymmetric unit of the compound there exist two independent molecules which are connected by weak aromatic CH…O hydrogen bonding between C(47)?H(47) and atom O(7) of the carbonyl group.

关键词： salicylic chloride salicylic amide polyester macrocycle crystal structure

维普期刊数据库

Zinc up-regulated the expression of the rice metallonthionein gene family and enhanced the zinc tolerance of yeast cells

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Progress in Natural science:Materials International 2007年第10期17卷 1166-1171页

作者： Xu Yufeng, Zhou Gongke, Li Yiqin and Liu Jinyuan(laboratory of molecular Biology and Protein science laboratory of the ministry of education, Department of Biological sciences and Biotechnology, Tsinghua University, beijing 100084, china) laboratory of molecular Biology and Protein science laboratory of the ministry of education Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Northern blot and functional complementation assay were employed to analyze the effects of zinc on expression of ten rice metallothionein genes (OsMT-Is) in rice seedlings and the growth of yeast cells transformed with OsMT-Is. Northern blot revealed that in shoots of the rice seedlings treated with different Zn2+concentrations, expression of most members of OsMT-I family was increased, except the type 4 OsMT-Is (OsMT-I-4a, 4b and 4c). In roots, Zn2+significantly increased the transcription of OsMT-I-1b and OsMT-I-2c, but reduced the trascription of OsMT-I-1a and OsMT-I-3a. When these ten cDNAs were heterologously expressed in zinc sensitive yeast mutant, all transgenic yeasts showed increased tolerance to Zn2+, and zinc accumulation in these yeast cells also increased. These indicated that OsMT-I family members might respond to extra Zn2+, and they could enhance Zn2+ tolerance of cells by direct binding Zn2+.

关键词： rice metallothionein induced expression Zn2+ tolerance.

Biomimetic preparation of elastomeric fibers with micro/nano structures on the surfaces

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Progress in Natural science:Materials International 2012年第5期22卷 493-501页

作者： Quanyong Liu a,Liqun Zhang c,Lei Jiang a,b a key laboratory of Bio-Inspired Smart Interfacial science and Technology of ministry of education,School of Chemistry and Environment,Beihang University,beijing 100191,PR china b beijing National laboratory for molecular sciences(MNLMS),key laboratory of Organic Solids,Institute of Chemistry,Chinese Academy of sciences,beijing 100190,PR china c State key laboratory of Organic-Inorganic Composites,beijing University of Chemical Technology,beijing 100029,PR china [a] key laboratory of Bio-Inspired Smart Interfacial science and Technology of ministry of education School of Chemistry and Environment Beihang University Beijing 100191 PR China [b] beijing National laboratory for molecular sciences (MNLMS) Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences Beijing 100190 PR China [c] State key laboratory of Organic–Inorganic Composites Beijing University of Chemical Technology Beijing 100029 PR China

Bioinspired by the spinning of spider silks,the biomimetic preparation of elastomeric fibers with micro/nano structures on the surfaces was attempted,and as a result,three types of ultrafine fullvulcanized powdered nitrile-butadiene rubber(UFPNBR)/thermoplastic polyurethane(TPU) fibers were *** first fiber was only decorated by the micron-sized grooves on the surface,and the second fiber was dotted by both the micron-sized grooves and nanometer-sized spheres on the surface,while the third fiber was helical and with a concave-convex *** biomimetic preparation mainly consisted of four steps,and the formation mechanism was described as an integrated mechanism of diffusion,coagulation,self assembly,and microphase *** micro/nano structures on the fibers were controlled by changing the mass ratio of UFPNBR to TPU and environment of vertical *** UFPNBR/TPU elastomeric fibers were hoped to have an integrated function of superhydrophobicity,self cleaning,and mechanical improvement of toughness or strength.

关键词： Biomimetic preparation Elastomeric fiber Micro/nano structure Self assembly Spider silk

A scheme to implement the Deutsch-Josza algorithm on a superconducting charge-qubit quantum computer

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Progress in Natural science:Materials International 2006年第6期16卷 594-599页

作者： HUO Wenyi and LONG Guilu (key laboratory for Quantum Information and Measurements, and Department of Physics, Tsinghua University, beijing 100084, china key laboratory for Atomic and molecular Nanosciences, Tsinghua University, beijing 100084, china) [a]key laboratory for Quantum Information and Measurements and Department of Physics Tsinghua University Bejjing China [b]key laboratory for Atomic and molecular Nanosciences Tsinghua University Bejing China

We have studied the implementation of the Deutsch-Josza quantum algorithm in a superconducting charge-qubit quantum computer. Different from previous studies, we have used the inductance coupled system of You et al. The detailed pulse sequences have been designed for the four possible functions in a 2-qubit system. The result is generalized to an arbitrary n-qubit system. This scheme will be useful for practical implementation of the algorithm.

关键词： superconducting quantum computer Deutsch-Josza algorithm.