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Stearic acid protects primary cultured cortical neurons against oxidative stress

Acta Pharmacologica Sinica 2007年第3期28卷 315-326页

作者： Ze-jian WANG~2 Cui-ling LIANG~3 Guang-mei LI~2 Cai-yi YU~2 Ming YIN~(2,4)2 School of Pharmacy,shanghai Jiao Tong university,shanghai 200030,china 3 institute of population studies,east china normal university,shanghai 2.0062.china School of Pharmacy Shanghai Jiao Tong University Shanghai China institute of population studies East China Normal University Shanghai China

Aim:To observe the effects of stearic acid against oxidative stress in primarycultured cortical ***:Cortical neurons were exposed to glutamate,hydrogen peroxide（H2O2）,or NaN3insult in the presence or absence of *** viability of cortical neurons was determined by MTT assay and *** antioxidant enzymes activity[superoxide dismutases（SOD）,glutathione peroxidase（GSH-Px）,and catalase（CAT）] and lipid peroxidation incultured cortical neurons were evaluated using commercial kits.{3-[l（p-chloro-benzyl）-5-（isopropyl）-3-t-butylthiondol-2-yl]-2,2-dimethylpropanoic acid,Na}[MK886;5 μmol/L;a noncompetitive inhibitor of proliferator-activated receptor（PPAR）α],bisphenol A diglycidyl ether（BADGE;100 μmol/L;an antagonist ofPPARγ）,and cycloheximide（CHX;30 μmol/L,an inhibitor of protein synthesis）were tested for their effects on the neuroprotection afforded by stearic *** blotting was used to determine the PPARγprotein level in cortical ***:Stearic acid dose-dependently protected cortical neurons againstglutamate or H202 injury and increased glutamate uptake in cultured *** protection was concomitant to the inhibition of lipid peroxidation and to thepromotion activity of Cu/Zn SOD and CAT in cultured cortical *** effects were completely blocked by BADGE and *** with H202 for 24 h,the expression of the PPARγprotein decreasedsignificantly（P<0.05）,and the inhibitory effect of H2O2on the expression of PPARγcan be attenuated by stearic ***:Stearic acid can protect corticalneurons against oxidative stress by boosting the internal antioxidant *** neuroprotective effect may be mainly mediated by the activation of PPARγandnew protein synthesis in cortical neurons.

关键词： oxidative stress receptors peroxisome prolilferator-activated neurons antioxidants

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Identification of human dopamine D1-like receptor agonist using a cellbased functional assay

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Acta Pharmacologica Sinica 2005年第10期26卷 1181-1186页

作者： Nan JIANG~2, Ke-qing OU-YANG~3, Shao-xi CAI~3, Ying-he HU~2, Zhi-liang XU~(2,4) ~2 shanghai institute of Brain Functional Genomics and Key Lab of Brain Functional Genomics, Ministry of Education, Science and Technology Commission of shanghai Municipality, east china normal Uuiversitv, shanghai 2.0062 ~3 College of Bioengineering, Chongqing university, Chongqing 400044, china shanghai institute of Brain Functional Genomics and Key Lab of Brain Functional Genomics Ministry of Education Science and Technology Commission of Shanghai Municipality East China Normal University Shanghai China College of Bioengineering Chongqing University Chongqing China

Aim: To establish a cell-based assay to screen human dopamine D1 and D5 receptor agonists against compounds from a natural product compound library. Methods: Synthetic responsive elements 6xcAMP response elements （CRE） and a mini promoter containing a TATA box were inserted into the pGL3 basic vector to generate the reporter gene construct pCRE/TA/Luci. CHO cells were co-trans- fected with the reporter gene construct and human D1 or D5 receptor cDNA in mammalian expression vectors. Stable cell lines were established for agonist screening. A natural product compound library from over 300 herbs has been established. The extracts from these herbs were used for human D1 and D5 receptor agonist screenings. Results: A number of extracts were identified thatactivated both D1 and D5 receptors. One of the herb extracts, SBG492, demonstrated distinct pharmacological characteristics with human D1 and D5 receptors. The EC50values of SBG492 were 342.7 μg/mL for the D1 receptor and 31.7 μg/mL for the D5 receptor. Conclusion: We have established a cell-based assay for high-throughput drug screening to identify D1-like receptor agonists from natural products. Several extracts that can active D1-like receptors were discovered. These compounds could be useful tools for studies onthe functions of these receptors in the brain and could potentially be developed into therapeutic drugs for the treatment of central nervous system diseases.

关键词： dopamine DI receptors drug screening Chinese herbal drugs

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NMR studies ON POLYETHER-POLYURETHANE ZWITTERIONOMERS——CHAIN STRUCTURE AND PHASE SEPARATION

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Chinese Journal of Polymer Science 1986年第1期4卷 72-77页

作者：王源身郭鸣明 Department of Chemistry Nanjing university Nanjing Analytical Center NMR group east china normal university shanghai china Nanjing institute of Material Science Fudan university china.

The 1H and 13C NMR spectra of polyether-polyurethancs （A） based on 4, 4’-diphenylmethanediisocyanate （MDI）, N-methyl diethanolamine （MDEA）, and polytetramethyleneoxide （PTMO）and the zwitterionomers derived from A with various degree of ionization was assigned by comparingwith model compounds. Phase separation of the samples was visualized through pulsed NMR investigations. The FIDsignals of these multiphase elastomers were recorded and decomposed by non-linear least squaresregression into fast Gaussian and slow exponential components assodated with the hard, the soft andthe interface respectively. Thus pulsed NMR gives both qualitative and quantitative informationabout the existence of multiphases and the relative amounts of materials in each phase.

关键词： NMR studies ON POLYETHER-POLYURETHANE ZWITTERIONOMERS CHAIN STRUCTURE AND PHASE SEPARATION

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Experimental Study of Motion of Nitrogen Taylor Bubbles and Liquid Slugs in Inclined Tubes

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Chinese Journal of Aeronautics 2009年第4期22卷 349-354页

作者： Zhang Hua,Wang Shuhua,Wang Jing institute of Engineering Thermo-physics,shanghai Jiao Tong university,shanghai 200240,china institute of Engineering Thermo-physics Shanghai Jiao Tong University Shanghai 200240 China

This article studies rising velocity of Taylor bubbles and liquid slugs in liquid nitrogen at different axial positions in upward inclined tubes by means of a high speed motion analyzer. The bottom-closed tubes in the experiments are 1.0 m long with an inner diameter of 0.014 m or 0.018 m. The tube inclines upward from 0~ to 50~ with respect to the normal. Statistical method is used to analyze the data of the Taylor bubble and the liquid slug velocity. Reflecting the effects of the inclination angle on the rising velocity of Taylor bubbles and liquid slugs, the experimental results indicate the similar trend the Taylor bubble velocity and the liquid slug velocity have： it increases first, and then decreases with the increase of the inclination angle. Moreover, with the increase of the inclination angle, the liquid slug velocity becomes greater than Taylor bubble velocity.

关键词： cryogenic two-phase flow rising velocity high speed motion analyzer liquid nitrogen Taylor bubble liquid slug inclined tube

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Numerical study on residual current and its impact on mass transport in the Hangzhou Bay and the Changjiang Estuary Ⅱ. Residual current and its impact on mass transport in winter

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Acta Oceanologica Sinica 2001年第1期20卷 153-170页

作者： Zhu Shouxian, Dang Pingxing, Shi Fengyan, Zhu Jianrong ( State Key Laboratory of Estuarine and Coastal research, east china normal university, shanghai 2.0062, china Meteorology institute of the PLA Science and Engineering university, Nanjing 211101, Ch State Key Lab. Estuarine Coast. Res. East China Normal University Shanghai 200062 China Meteorology institute PLA Sci. and Engineering University Nanjing 211101 China

Some observational characteristics of residual current and mass transport in the Hangzhou Bay and the Changjiang Estuary in winter are analyzed. The residual current and its impact on mass transport are simulated with a 3 - D joint model for the Hangzhou Bay and the Changjiang Estuary, in which the impacts of river flux, wind, baroclinic pressure gradient (BPG), background current in the east china Sea and tide (including M2, S2, K1 and O1) are taken into account. Based on there studies, further simulations are made to analyze the dynamical mechanisms of the observational characteristics.

关键词： The Hangzhou Bay the Changjing Estuary residual current mass transport numerical study winter

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上海低龄失业群体现状分析与问题思考

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市场与人口分析 2003年第6期9卷 21-26页

作者：杨丽华东师范大学人口研究所上海200062

利用上海市第五次人口普查资料 1 0 %抽样调查数据 ,分析 2 0～ 39岁低龄群体的失业问题。通过透视“2 0 30”1 低龄失业群体的特征 ,挖掘隐藏在其背后的失业原因。

关键词：上海低龄失业群体人口普查失业问题年龄性别

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基于普查数据的中国劳动适龄人口分析

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市场与人口分析 2003年第6期9卷 33-38,66页

作者：张善余华东师范大学人口研究所上海200062

基于 2 0 0 0年人口普查数据 ,对中国劳动适龄人口的基本态势作了分析和概括 ,主要涉及劳动适龄人口的数量、增长率、自然结构、受教育程度以及劳动适龄人口分布与投资分布相互匹配的关系。中国劳动适龄人口总量庞大 ,增长迅速 ,其性别... 详细信息

基于 2 0 0 0年人口普查数据 ,对中国劳动适龄人口的基本态势作了分析和概括 ,主要涉及劳动适龄人口的数量、增长率、自然结构、受教育程度以及劳动适龄人口分布与投资分布相互匹配的关系。中国劳动适龄人口总量庞大 ,增长迅速 ,其性别比下降 ,中位年龄增大 ,受教育程度不高 ,且存在着悬殊的地区差异。如何化解劳动适龄人口的巨大压力 ,改善就业状况。

关键词：人口普查数据中国劳动适龄人口地区差异人口数量受教育程度人口分布

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Relationships between bacterial-algal proliferating and mass extinction in the Late Devonian Frasnian-Famennian transition:Enlightening from carbon isotopes and molecular fossils

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Science china Earth Sciences 2005年第10期48卷 1656-1665页

作者： GONG Yiming1,2, XU Ran1, TANG Zhongdao1, SI Yuanlan3 & LI Baohua4 1. Faculty of Earth Science and State Key Laboratory of Geological Processes and Mineral Resources, china university of Geosciences, Wuhan 430074, china 2. institute of Resources & Environments, Henan Polytechnic university, Jiaozuo 454003, china 3. Department of Computer Science, South-Central university for Nationalities, Wuhan 430074, china 4. Laboratory of Marine Geology, Tongji university, shanghai 200092, china china university of Geosciences Henan Polytechnic university South-Central university for Nationalities Tongji university

studies show positive shifts of inorganic and organic carbon isotope values (δ 13Ccarb and δ 13Ckerogen) from +0.43 (‰ V-PDB) to +3.54 (‰ V-PDB) and from ?29.38 (‰ V-PDB) to ?24.14 (‰ V-PDB), respectively, B* (Ba* = Ba/ (Al2O3 X 15%)) values from 0.015 to 0.144, TOC values from 0.02% to 0.21%, V/Cr values from 0.3 to 2.0, Sr/Ba values from 3.20 to 49.50 in the Late Devonian Frasnian Upper rhenana zone to the top linguiformis zone of the Yangdi sec-tion deposited in carbonate slope facies of Guilin, Guangxi, South china, which indicates that biomass, productivity, organic carbon burial and salinity increase and that oxygenation near the boundary between sediments and waters decreases from the Late Devonian Frasnian Upper rhenana zone to the top linguiformis zone. Abundance of molecular fossils increases and normal alkanes, isoprenoid hydrocarbon, terpanes and steranes are dominated from the Late Devonian Frasnian to the bottom of Famennian, which shows that the predecessors of molecular fossils of the Frasnian-Famennian (F-F) transition are dominated by marine phytoplankton, zooplankton and benthic bacteria with no photosynthesis. Therefore, it is considered that the F-F transitional mass extinction with a multistage, selection and global synchronizing was caused by bacte-rial-algal proliferating, continuing deterioration of the shallow marine ecoenvironment of the mid-dle-lower latitudes. A simple cause and effect chain can be expressed as: appearance of seed plants and multi-storied forests → enhanced chemical and biochemical weathering and pe-dogenesis → wide development of soils → increasing riverine nutrient fluxes in epicontinental sea → from superoligotrophic to eutrophic in epicontinental sea → proliferating of marine phyto-plankton and zooplankton → frequent red tide and anoxia → mass extinction of shallow marine organisms in the middle-lower latitudes. It is worth notice that the factor drawdown of atmos-pheric Pco2, climatic cooling and sea level falling c

关键词： mass extinction, bacteria-algae, carbon isotopes, molecular fossils, biodiversity, Late Devonian, Guangxi, South china.

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Reconstruction of the most recent common ancestor sequences of SARS-Cov S gene and detection of adaptive evolution in the spike protein

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Chinese Science Bulletin 2004年第12期49卷 1311-1313页

作者： ZHANG Yuan1, ZHENG Nan2,3, HAO Pei4,5 & ZHONG Yang6 1. Laboratory of Systematic and Evolutionary Botany, institute of Bot- any, Chinese Academy of Sciences, Beijing 100093, china 2. College of Life Sciences, Beijing normal university, Beijing 100875, china 3. institute of Viral Disease Control and Prevention, Chinese CDC, Beijing 100052, china 4. Bioinformation Center, shanghai institutes for Biological Sciences, Chinese Academy of Sciences, shanghai 200031, china 5. shanghai Center for Bioinformation Technology, shanghai 200235, china 6. School of Life Sciences, Fudan university, shanghai 200433, china 1Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences 100093 Beijing China. 2College of Life Sciences Beijing Normal University 100875 Beijing China. 3institute of Viral Disease Control and Prevention Chinese CDC 100052 Beijing China. 4Bioinformation Center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 200031 Shanghai China. 5shanghai Center for Bioinformation Technology 200235 Shanghai China. 6School of Life Sciences Fudan University 200433 Shanghai China.

The genome organization and expression strategy of severe acute respiratory syndrome coronavirus (SARSCoV) have been described extensivelyl1- 10]. As a structural glycoprotein on the virion surface, the spike protein is responsible for binding to host cellular receptors and for the fusion between the viral envelope and the cellular membrane. It also induces neutralizing antibodies in the host and mediates cellular immunity[11]. Previous studies suggested that amino acid replacements in the spike protein could dramatically alter the pathogenesis and virulence of some coronaviruses[11]. It is therefore reasonable to test the hypothesis that radical amino acid replacements in the spike protein, favored by environmental selective pressure during the process of SARS-CoV interspecific transmission[10], might make this pathogen adapt to a new host. In this study, we investigated a total of 108complete sequences of the SARS-CoV S gene from GenBank (until March 23, 2004). After omission of those records containing frame-shift mutations or low quality sequences, e.g. ZJ01, and selection of one sequence for identical records, an alignment of 42 sequences was obtained using the program Clustal-X[13]. Then, we reconstructed the most recent common ancestor (MRCA) sequences of the SARS-CoV S gene and detected the adaptive evolution in the spike protein.

关键词： SARS冠状病毒自适应进化基因表达糖蛋白分子免疫学波峰病毒

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Telomere and telomerase in oncology

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Cell Research 2002年第1期12卷 1-7页

作者： JIAO MU, Li XIN WEI International Joint Cancer institute, Second Military Medical university, shanghai 200433, china International Joint Cancer institute Second Military Medical University Shanghai China

Shortening of the telomeric DNA at the chromosome ends is presumed to limit the lifespan of human cells and elicit a signal for the onset of cellular senescence. To continually proliferate across the senescent checkpoint, cells must restore and preserve telomere length. This can be achieved by telomerase, which has the reverse transcriptase activity. Telomerase activity is negative in human normal somatic cells but can be detected in most tumor cells. The enzyme is proposed to be an essential factor in cell immortalization and cancer progression. In this review we discuss the structure and function of telomere and telomerase and their roles in cell immortalization and oncogenesis. Simultaneously the experimental studies of telomerase assays for cancer detection and diagnosis are reviewed. Finally, we discuss the potential use of inhibitors of telomerase in anti-cancer therapy.

关键词： Telomere telomerase cancer telomerase assay inhibitor.

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