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Genome-wide linkage disequilibrium and recombination map in rice

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Genome-wide linkage disequilibrium and recombination map in ...

第十三届全国植物基因组学大会

作者： Kunyan Liu,Bin Han~* National Center For Gene Research,institute of plant physiology and ecology.shanghai institute For biological sciences.chinese academy of sciences.shanghai 200233,china

Linkage disequilibrium(LD),the nonrandom association between alleles at different loci,is a noticeable feature of the organization of genetic variations in natural *** knowledge of LD facilitates the determination of ...

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Large-scale genome sequencing based genotyping and genome-wide association studies in rice

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Large-scale genome sequencing based genotyping and genome-wi...

第十一届全国植物基因组学大会

作者： Bin Han National Center for Gene Research & institute of plant physiology and ecology.shanghai institutes of biological sciences.chinese academy of sciences.Beijing institute of Genomics,CAS

Rice(Oryza sativa L.) is the staple food for more than half of the world *** thousands of years of rice cultivation,china has accumulated an enormously diverse collection of landraces. This is an invaluable genetic re...

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Development of Transgenic Sweet Potato(Ipomoea batatas) plants Tolerant to Salt Stress

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Development of Transgenic Sweet Potato(Ipomoea batatas) Plan...

植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Weijuan Fan and Peng Zhang~* shanghai Center for Cassava Biotechnology,institute of plant physiology & ecology.shanghai institutes for biological sciences.chinese academy of sciences.300 Fenglin Road,shanghai 2.0032.china

Sweet potato is a herbaceous perennial root crop and provides major feedstock for food and biofuel industries in *** stresses,such as salinity,drought and low temperature,are critical delimiters for high productivity ... 详细信息

关键词： sweet potato over-expression Na+/H+ antiporter betaine aldehyde dehydrogenase

利用DNA家族重排提高青霉素G酰化酶合成活力(英文)

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生物化学与生物物理学报 2003年第6期35卷 573-579页

作者：周政张爱晖王晶茹陈茂林李仁宝杨晟袁中一中国科学院上海生命科学院研究院生物化学与细胞生物学研究所中国科学院上海生命科学研究院植物生理生态研究所中国科学院上海生命科学院研究院生物化学与细胞生物学研究所上海200031 上海2.0032

NA家族重排技术是酶定向进化的有力工具 ,已在实际应用中获得了巨大成功。来源于Providenciarettgeri、Escherichiacoli和Kluyveracitrophila的青霉素酰化酶基因序列同源性为 6 2 .5 %～ 96 .9%。在Providenciarettgeri青霉素酰化酶基... 详细信息

NA家族重排技术是酶定向进化的有力工具 ,已在实际应用中获得了巨大成功。来源于Providenciarettgeri、Escherichiacoli和Kluyveracitrophila的青霉素酰化酶基因序列同源性为 6 2 .5 %～ 96 .9%。在Providenciarettgeri青霉素酰化酶基因克隆和表达的基础上 ,利用DNA家族重排技术构建了上述基因的嵌合体突变库。通过平板初筛获得有活力的阳性克隆 ,表达提取突变酶测定其合成水解活力比。对突变酶进行随机测序的结果表明多基因嵌合体突变库显示出明显的多样性。通过一轮重排及筛选 ,获得了合成活力提高 4 0 %的突变酶 ,并且发现α亚基的重排对酶合成活力的提高更加有效。上述方法的应用有望获得合成活力进一步提高的青霉素G酰化酶。

关键词：青霉素酰化酶 DNA家族重排合成水解比

A Study of Transmitter-on ETR1 and ERS1 Signaling Implies Differential Collaborations of Arabidopsis Ethylene Receptors

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A Study of Transmitter-on ETR1 and ERS1 Signaling Implies Di...

植物分子生物学与现代农业——2010全国植物生物学研讨...

作者： Qian Liu and Chi-Kuang Wen~* National Key Laboratory of plant Molecular Genetics,institute of plant physiology and ecology. shanghai institutes for biological sciences.chinese academy of sciences.shanghai 2.0032.china

Ethylene responses are modulated by a family of five ethylene receptor members in *** the five receptor members may function individually or collaboratively remains *** addressed this question by examining if transmit...

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Preparation of Ethylene Gas and Comparison of Ethylene Responses Induced by Ethylene,ACC,and Ethephon

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Preparation of Ethylene Gas and Comparison of Ethylene Respo...

植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Wei Zhang,Chi-Kuang Wen~* National Key Laboratory of plant Molecular Genetics,institute of plant physiology and ecology. shanghai institutes for biological sciences.chinese academy of sciences.shanghai 2.0032.china

Ethylene is a gaseous plant hormone used in many physiological studies examining its role in plant growth and ***,ethylene gas may not be conveniently available to many laboratories for occasional use,and therefore se...

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Bacterial Type Ⅰ Glutamine Synthetase of the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32,is the Only Enzyme Responsible for Glutamine Synthesis under Physiological Conditions

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Acta Biochimica et Biophysica Sinica 2006年第12期 821-830页

作者： Wen-Tao PENG~(1&) Jin WANG~1 Ting WU~2 Jian-Qiang HUANG~1 Jui-Shen CHIAO~1 Guo-Ping ZHAO~(1,3,4) 1 Laboratory of Molecular Microbiology,institute of plant physiology and ecology.shanghai institutes for biological sciences.chinese academy of sciences.shanghai 2.0032.china 2 Department of Medicinal Chemistry,School of Pharmacy,Fudan University,shanghai 2.0032.china 3 State Key Laboratory of Genetic Engineering/Department of Microbiology,School of Life Science,Fudan University,shanghai 200433,china 4 Laboratory of Health and Disease Genomics,chinese National Haman Genome Center at shanghai,shanghai 201203,china

The structural gene for glutamine synthetase,glnA,from Amycolatopsis mediterranei U32 wascloned via screening a genomic library using the analog gene from Streptomyces *** clone wasfunctionally verified by complementing for glutamine requirement of an Escherichia coli glnA null mutantunder the control of a lac *** analysis showed an open reading frame encoding a protein of466 amino acid *** deduced amino acid sequence bears significant homologies to other bacterialtype Ⅰ glutamine synthetases,specifically,71% and 72% identical to the enzymes of *** and Myco-bacterium tuberculosis,*** of this glnA gene in *** U32 led to glutamineauxotrophy with no detectable glutamine synthetase activity in *** contrast,the cloned glnA+gene cancomplement for both phenotypes in *** thus suggested that in *** U32,the glnA geneencoding glutamine synthetase is uniquely responsible for in vivo glutamine synthesis under our laboratorydefined physiological conditions.

关键词： glnA glutamine synthetase Amycolatopsis mediterranei rifamycin SV

Structural Characterization and Expression Analysis of the PLT Gene Family in Oryza sativa

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Structural Characterization and Expression Analysis of the P...

植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Peng Li,Hong-Wei Xue~* National Key Laboratory of plant Molecular Genetics,institute of plant physiology and ecology. shanghai institutes for biological sciences.chinese academy of sciences.shanghai 2.0032.china

PLT genes encoding AP2-domain transcription factors and has been shown to be required for QC identity and specification early in root development in dicot *** searching the database and structural analysis,we identifi... 详细信息

关键词： Oryza sativa OsPLT root

集胞蓝藻PCC6803含疏水亚基的NAD(P)H脱氢酶亚复合体的分离

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生物化学与生物物理学报 2003年第8期35卷 723-727页

作者：邓勇叶济宇米华玲沈允钢中国科学院上海生命科学研究院植物生理生态研究所、植物分子遗传国家重点实验室中国科学院上海生命科学研究院植物生理生态研究所、植物分子遗传国家重点实验室上海2.0032

利用离子交换与凝胶过滤层析 ,从n dodecylβ D maltoside(DM)处理的集胞蓝藻SynechocystisPCC6 80 3细胞粗提液中 ,首次分离到两个包含NDH疏水亚基NdhA的亚复合体。酶活性分析表明 ,分离到的NDH亚复合体具有NADPH 氮蓝四唑 (NBT)氧化... 详细信息

利用离子交换与凝胶过滤层析 ,从n dodecylβ D maltoside(DM)处理的集胞蓝藻SynechocystisPCC6 80 3细胞粗提液中 ,首次分离到两个包含NDH疏水亚基NdhA的亚复合体。酶活性分析表明 ,分离到的NDH亚复合体具有NADPH 氮蓝四唑 (NBT)氧化还原酶活性 ,以NADPH为电子供体可以还原铁氰化钾、二溴百里香醌 (DBMIB)、二氯酚靛酚 (DCPIP)、duroquinone以及UQ 0等质醌类电子受体。

关键词：集胞蓝藻 NAD(P)H脱氢酶疏水亚基