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Total Synthesis of Przewaquinone A

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chinese Chemical Letters 1997年第3期8卷 205-206页

作者： Wen Hu DUAN Jun Chao CAI (shanghai institute of Materia Medica, chinese academy of sciences, shanghai 200031) chinese ACAD SCI SHANGHAI INST MAT MEDSHANGHAI 200031PEOPLES R CHINA

Przewaquinone A, a natural orthoquinone was synthesized through several steps, the analytic data of the synthetic target compound was identical with that of przewaquinone A.

关键词： HRMS HNMR Total Synthesis of Przewaquinone A

Regulation of mammalian pre-mRNA splicing

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Science china(Life sciences) 2009年第3期52卷 253-260页

作者： HUI JingYi State Key Laboratory of Molecular Biology,institute of Biochemistry and Cell Biology,shanghai institutes for Biological sciences,chinese academy of sciences,shanghai 200031,china 1. State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

In eukaryotes,most protein-coding genes contain introns which are removed by precursor messenger RNA(pre-mRNA) *** splicing is a process by which multiple messenger RNAs(mRNAs) are generated from a single pre-mRNA,resulting in functionally distinct *** genome-wide analyses of alternative splicing indicated that in higher eukaryotes alternative splicing is an important mechanism that generates proteomic complexity and regulates gene ***-regulation of splicing causes a wide range of human *** review describes the current understanding of pre-mRNA splicing and the mechanisms that regulate mammalian pre-mRNA *** also discusses emerging directions in the field of alternative splicing.

关键词： pre-mRNA splicing alternative splicing splicing regulation signal transduction microRNA

Studies on the Crinum Alkaloids part Ⅱ~[1]: Synthes is of Crinasiadine and Trisphaeridine

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chinese Chemical Letters 1997年第6期8卷 493-496页

作者： Hua Wu SHAO Jun Chao CAI (shanghai institute of Nateria Medica, chinese academy of sciences, shanghai, 200031) shanghai institute of Materia Medica Chinese Academy of Sciences Shanghai 200031 China

Crinasiadine 1 has been easily synthesized by palladium acetate catalyzed cyclization of N- (o-bromophenyl) -3, *** synthesis trisphearidine 8 with a practical type of palladium chloride catalyzed cyclization as th... 详细信息

关键词： OCH Synthesis of Crinasiadine and Trisphaeridine ppm Studies on the Crinum Alkaloids part

Construction of normal human IgE phage antibody library and the screening of the anti-trichosanthin IgE

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Cell Research 1996年第1期6卷 1-9页

作者： ZAN HONG MING YEH (shanghai institute of Cell Biology, chinese academy of sciences, shanghai 200031, china.) shanghai institute of Cell Biology Chinese Academy of Sciences Shanghai 200031 China.Received 3April1996 Revised 11June1996 Accepted 12June1996

Allergen specific IgE response is the major cause of immediate hypersensitivity. However the number of IgEproducing B cells and the amount of IgE, especially the specific IgE, are so low, it greatly impedes the study of the allergic-specifc antibody responses. Here we report the construction of a normal human IgE combinatorial library The repertoire of IgE VH genes and of K genes were separately amplified from normal human peripheral blood lymphocytes through RT-PCR, and were then constructed to form the phage surface display human Fab(IgEVH) library. A plant protein allergen, trichosanthin(TCS), was used to affinity-enrich and to screen the anti-TCS phage HuFab clones from the library. Human IgE(Fab) to TCS were detected.

关键词： Phage antibody library human IgE(Fab) human anti-trichosanthin IgE

Cleavage of Supercoiled Circular Double-stranded DNA Induced by a Eukaryotic Cambialistic Superoxide Dismutase from Cinnamomum camphora

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 609-617页

作者： Bao-Zhong WANG, Xu-Bin WEI&, and Wang-Yi LIU* State Key Laboratory of Molecular Biology, institute of Biochemistry and Cell Biology, shanghai institutes for Biological sciences,chinese academy of sciences, shanghai 200031, china State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

A eukaryotic cambialistic superoxide dismutase (SOD) has been purified to homogeneity from mature seeds of the disease- and insect-resistant camphor tree (Cinnamomum camphora). Besides the known role of this SOD in protecting cells against oxidative stress, it can induce the cleavage of supercoiled double-stranded DNA into nicked and linear DNA. It can not cleave linear DNA or RNA, demonstrating there is no DNase or RNase in the purified cambialistic SOD. Furthermore, the SOD can linearize circular pGEM-4Z DNA that is relaxed by topoisomerase I. This result indicates that the DNA-cleaving activity re- quires substrates being topologically constrained. The supercoiled DNA-cleaving activity of the cambialistic SOD can be inhibited by either SOD inhibitor (azide) or catalase and hydroxyl radical scavengers (ethanol and mannitol). The chelator of iron, diethylenetriaminepentaacetic acid (DTPA), also inhibits the supercoiled DNA-cleaving activity. These results show that the dismutation activity is crucial for the supercoiled DNA cleavage. The modification of tryptophan residue of the cambialistic SOD with N-bromosuccinimide (NBS) shows that these two activities are structurally correlative. The reaction mechanism is proposed that the hydroxyl radical formed in a transition-metal-catalyzing Fenton-type reaction contributes to the DNA- cleaving activity. In addition, the cleavage sites in supercoiled pGEM-4Z DNA are random.

关键词： cambialistic SOD Fenton-type reaction hydroxyl radical relaxed circular duplex DNA supercoiled DNA cleavage

Formation and Oxidation Resistance of Silicide Coatings for Mo and Mo-Based Alloys

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Journal of Materials Science & Technology 2001年第4期17卷 479-481页

作者： Yu WANG , Yunpeng LI and Xingfang HU shanghai institute of Ceramics, chinese academy of sciences, shanghai 200050, china chinese Acad Sci Shanghai Inst Ceram Shanghai 200050 Peoples R China

The forming process of silicide coatings on pure Mo and Mo-base alloys, obtained by the gas- phase deposition method. has been studied by examining the microstructure of coatings and the relationship between coating thickness and process parameters. It was shown that the growth of coatings was diffusion-controlled, the diffusion of silicon to be coated into Mo or Mo-base alloys was mainly responsible for the formation of silicide. The relationship between initial silicide thickness and oxidation resistance was also investigated, and the equation of service life of the coatings at high temperature in air is presented.

关键词： Mo Formation and Oxidation Resistance of Silicide Coatings for Mo and Mo-Based Alloys

Visual neuroscience research in china

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Science china(Life sciences) 2010年第3期53卷 363-373页

作者： YAO HaiShan,LU HaiDong & WANG Wei institute of Neuroscience,State Key Laboratory of Neuroscience,shanghai institutes for Biological sciences,chinese academy of sciences,shanghai 200031,china 1. institute of Neuroscience State Key Laboratory of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Historically,vision research in china was one of a few distinct research programs within the chinese academy of sciences(CAS).With improved funding opportunities and research environment in neuroscience,vision research at several research institutes within the academy has made significant progress not only in the quantity of publications,but also in the quality of the *** on our own expertise,this review is mainly focused on the findings that have advanced the understanding of visual processing in the central visual pathway,visual perceptual learning,visual development and eye diseases.

关键词： visual cortices contextual modulation feedback feature binding motion processing perceptual learning functional brain imaging eye movement visual development and senescence

Identification of γ1 subunit of GABAA receptor in rat testis

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Cell Research 2002年第1期12卷 33-37页

作者： JING HUA HU, YUAN CHANG YAN Lab of Molecular Cell Biology, institute of Biochemistry and Cell Biology, shanghai institutes for Biological sciences, chinese academy of sciences, shanghai 200031, china 1Lab of Molecular Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Lab of Molecular Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

The isoform type ofγsubunits of GABAA receptor is a molecular determinant of its pharmacological characteristics. At present, the existence of GABAA receptor in mammalian sperm is still a controversy. By using degenerate primers designed according to highly conserved region in all threeγ(γ1, γ2 andγ3) subunits cloned in rat brain, we performed reverse transcription polymerase chain reaction (RT-PCR) to examine the expression pattern of -γsubunits of GABAA receptor in rat testis. Only one 370 bp fragment was obtained from RT-PCR in rat testis and sequencing results showed that it representedγ1 subunit, but notγ2 orγ3 subunit. Using the cloned fragment as probe, a 3.8 kb transcript which in size as same asγ1 ubunit in rat brain was detected in rat testis mRNA by performing Northern blot assay. Furthermore, results of in situ hybridization assay confirmed thatγ1 subunit was expressed in round spermatids and spermatozoa, maybe also in secondary spermatocyte. These evidences proved that 71 subunit of GABAA receptor is exclusively expressed in rat testis and this feature may be the structural basis of the specific function of GABAA receptors in sperm acrosome reaction.

关键词： GABAA receptor rat testis γ1 subunit.

Preparation and Characterization of Sol-gel Derived CH_3SiO_(3/2)-SiO_2 Thick Film

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Journal of Materials Science & Technology 1998年第6期14卷 523-526页

作者： Bing CAO and Congshan ZHU(shanghai institute of Optics and Fine Mechanics, chinese academy of sciences, shanghai 201800, china) shanghai institute of Optics and Fine Mechanics Chinese Academy of Sciences Shanghai 201800 China

A large size CH3SiO3/2-SiO2 self sustained film with the thickness ranging from 50 to 1000 μm was prepared through sol-gel method by adopting CH3Si(OC2H5)3 and Si(OC2H5) as *** this paper, the preparation processes of this thick film are discussed in detail and XRD technique was adopted for the identification of oligomer solution. Also, IR and AFM techniques were used to investigate the film structure and surface morphology

关键词： Preparation and Characterization of Sol-gel Derived CH3SiO MTES CH