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Different distributions of P2X4 and P2X7 receptors in the neurons of the spinal cord dorsal horn of rats with spared nerve injury

Different distributions of P2X4 and P2X7 receptors in the ne...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Rong CAO~1,Yan XU~2,Ying-Fei XIONG~1,Li DUAN~1,Ling-Feng TU~1,Zhi-Ren RAO~(1,*) 1 institute of neurosciences Fourth Military Medical University,Xi''an,710032 2 Dept.Neurology,Xijing Hospital.Fourth Military Medical University,Xi''an 710032

＜正＞Objective To research the locahzation of P2X4 and P2X7 receptors in the neurons of rat limber dorsal horn at 20 days after spared nerve injury（SNI）.Methods Male adult Sprague-Dawley rats were used for this study,SNI operation were performed,at 20 days after SNI,some lumbosacral segments were cut the formal sections（30 urn thickness） on a on a cryostat,these sections were stained with double labeling of anti-P2X4/NeuN or P2X7/*** lumbosacral segments were cut the formal sections（50μm thickness） on a vibrating microtome,anti-P2X4,anti-P2X7 immuno-elecromicroscopic staining with 1.4 nm NANOGOLD gold cluster label and enhanced with HQ SILVERTM enhancement kit（NANOPROBES, INC,NY） was *** *** using confocal microscopy,P2X4/NeuN or P2X7/NeuN double labeled neurons were observed in spinal cord dorsal *** showed that P2X4 positive gold cluster were mainly distribute in post-synaptic neuronal element,the endoplasmic reticulum of the soma and the membrane of mitochondrion, a few were also localize in pre-synaptic *** distribution of P2X7 positive gold cluster revealed difference with that of P2X4 receptor,P2X7 positive gold cluster were mainly localized in the pre-synaptic axonal terminals,the mitochondria cristae and the endoplasmic reticulum of the soma,a few also expressed in post- synaptic neuronal *** The different distributions of P2X4 and P2X7 receptors in the neurons may indicate they have different function.

关键词： spinal cord P2X4 receptors P2X7 receptors neuron rat

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Specific temporal-spatial distributions of P2X4 and P2X7 receptors in the spinal astrocytes and microglia contribute to the different phases of tactile allodynia after spared nerve injury

Specific temporal-spatial distributions of P2X4 and P2X7 rec...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Ying-Fei XIONG~1,Yan XU~2,Li DUAN~1,Rong CAO~1,Ling-Feng TU~1,Zhi-Ren RAO~(1,*) 1 institute of neurosciences,Fourth Military Medical University,Xi''an,710032. 2 Department of Neurology,Xijing Hospital,Fourth Military Medical University,Xi''an 710032

＜正＞Objective To research the difference of the localization and time course of astrocytic and microglial activation, P2X4 and P2X7 receptors expression in rat limber dorsal horn at 10,20 and 30 days after spared nerve injury（SNI）.Methods Male adult Sprague-Dawley rats were divided into control group,sham SNI group and SNI *** experimental animals survived for 10,20 and 30 days after *** this period,brilliant blue G（BBG,a selective antagonist of P2X 7 R）,pyridoxal-phosphae-6-azophenyl-2,4-disulphonicacid（PPADS,an antagonist of P2X1,2,3,5,7R subtypes but not P2X4R）,TNP-ATP（an antagonist of P2X4 R） and physiological saline were injected into subarachnoid space of the lumbosacral enlargement *** Paw withdrawal mechanical threshold（PWMT） was measured by using von Frey *** lumbosacral segment were performed immunofluorescence staining for glial fibrillary acidic protein （GFAP,labeling astrocyte） and /or 0X42（labeling microglia） combining with immunostaining of P2X4 or P2X7,western blots were performed at each time ***-GFAP/P2X4,anti-GFAP/P2X7,anti-OX42/P2X4,and Ox42/P2X7 double immuno-elecromicroscopic staining was performed by the lumbosacral dorsal horn at 20 days after ***（1） SNI surgery significantly reduced the PWMT value compared to which of the sham operation group or intact side.（2） 20 days after SNI,treating with BBG,PPADS and ANT-ATP markedly increased the PWMT value than that of the non-treated SNI group（P＜0.05）.（3）Immunofluorescence showed that the expression levels of GFAP,0X42,P2X4 and P2X7 in spinal cord dorsal horn of SNI side were significantly higher than the control *** of them were purinergic receptors double labeled astrocytes and *** astrocyte,P2X4R maintained at the basal level but P2X7R increased rapidly to a plateau from day 10 and sustained at this high level till the *** microglia,P2X4R showed a striking upregulation and peaked at day 20 then decreased steeply but P2X7R

关键词： neuropathic pain spinal cord P2Xs receptors astrocytes microglia rat

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The major histocompatibility complex genes were associated with the painrelated responses induced by formalin between DA and congenic DA.1U rats

The major histocompatibility complex genes were associated w...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Yuan GUO~1,Fan-Rong YAO~1,Dong-Yuan CAO~(1,2),Rong LUO~1,Li LI~1,Shao-Jie MA~1,Hui-Sheng WANG~1,Yan ZHAO~(1*) 1 Department of Physiology and Pathophysiology,Xi''an Jiaotong University School of Medicine,Xi''an,710061,china 2 Palmer Center for Chiropractic Research,Palmer College of Chiropractic,741 Brady Street,Davenport,I A 52803, USA

＜正＞Objective Some studies on humans,as well as laboratory animals suggested that there exist significant differences of pain-related response between *** in genetic influences on pain-related responses has increased dramatically in recent *** the present study,the role of major histocompability complex（MHC） genes in the pain-related responses induced by formalin was investigated in Dark-Agouti（DA） rats and a novel congenic DA.1U rats, whose genetic background is the same as DA rats except MHC *** Behaviors were simultaneously analyzed for lh and the specific nociceptive scores were measured using the method described by Dubuisson and Dennis（1977） following intraplantar injection of formalin（2.5%,50μL）.The discharge rates of single afferent fiber innervating the planta of hindpaw were recorded from dorsal root to investigate the response induced by *** methods were used to observe the expression of RT1B,which represented the DQ region of MHC II genes,in L4/5 spinal cord in the two rat strains to analyze the role of MHC genes in the pain-related ***（1） Biophasic pain-related responses were observed in the two strains following the intraplanta injection of *** specific nociceptive scores of DA rats in the two phases were significantly higher than those of DA.1U rats（P＜0.01）.（2） Electrophysiological results showed that the discharge rates of C and A8 fibers increased significantly in the two strains,and the mean net change of discharge rates of DA rats were significantly higher than that of DA.1U rats（P＜0.05）.（3） The expressions of RT1B were mainly observed in laminaeⅠ-Ⅱof L4/5 spinal cord in two *** expressions of RT1B in DA rats were significantly higher than those in DA.1U rats before and after injection of formahn（P＜0.05）.All these results suggested that DQ region of MHCⅡgenes may be involved in the pain-related response induced by formalin,which may provide further evidence for

关键词： major histocompability complex pain-related response individual difference RT1B

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BNIP3-AIF pathway is involved in hippocampal neuronal death induced by oxygen-glucose deprivation

BNIP3-AIF pathway is involved in hippocampal neuronal death ...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Shen-Ting ZHAO~1,Ming CHEN~1,Shu-Ji LI~1,Ming-Hai ZHANG~1,Bo-Xing LI~1,Xin-Hong ZHU~1,Ji-Ming KONG~(1,2),Tian-Ming GAO~(1,*) 1 Department of Neurobiology,Southern Medical University,Guangzhou 510515,china 2 Department of Human Anatomy and Cell Science,University of Manitoba Faculty of Medicine,Winnipeg,Man., Canada

＜正＞Objective Evidences have shown that mitochondrial protein apoptosis-inducing factor（AIF） is involved in caspase-independent neuronal apoptosis induced by ***,its upstream signaling mechanism remains *** we test the hypothesis that BNIP3 may be an upstream signal of AIF that mediates ischemic neuronal death. Methods We performed immunocytochemistry,Western blotting,cell transfection,subcellular fractionation,and RNA interfering to analyze the expression and localization of BNTP3 and AIF in primary cultured hippocampal neurons subjected to oxygen-glucose deprivation（OGD）.Results After OGD,both BNIP3 and AIF are *** translocates from mitochondria to nucleus in a time-dependent manner which follows the mitochondrial BNIP3 ***,the translocation of AIF occurs hours before the observable nuclear *** of BNIP3 induces nuclear translocation of AIF and the neuronal death whereas inhibition of BNIP3 decreases OGD-induced AIF translocation and neuronal ***3-induced neuronal death is also blocked by reducing AIF *** BNIP3-AIF pathway is involved in OGD-induced hippocampal neuronal death.

关键词： oxygen-glucose deprivation hippocampal neuron apoptosis BNIP3 AIF

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