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Science china(Life Sciences) 2008年第12期51卷 1094-1100页

作者： MIAO FengPing1,2, LU DaYan1, ZHANG ChengWu3, ZUO JinCheng4, GENG YaHong1, HU HongJun1 & LI YeGuang1, 1 Laboratory of Plant Biotechnology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, china 2 Laboratory of Coastal Zone Bioresources, Yantai institute of Costal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai 264003, china 3 institute of hydrobiology, jinan university, guangzhou 510632, china 4 College of Life Science, Ludong university, Yantai 264025, china Laboratory of Plant Biotechnology Wuhan Botanical Garden Chinese Academy of Sciences Wuhan China Laboratory of Coastal Zone Bioresources Yantai Institute of Costal Zone Research for Sustainable Development Chinese Academy of Sciences Yantai China institute of hydrobiology Jinan University Guangzhou China College of Life Science Ludong University Yantai China

The compositions and contents of astaxanthin esters and fatty acids in four types of Haematococcus pluvialis cells were studied by HPLC and GC-MS. Results showed that the synthesis and accumulation of astaxanthin was independent of the formation of cysts, but was highly correlated with the synthesis and accumulation of fatty acids, though it is an well known phenomenon that the accumulation of astaxanthin is usually accompanied by the formation of cyst. The red cysts contain more than 30% of fatty acids, with 81% of the unsaturated fatty acids. Taken together, besides a resource of astaxanthin, H. pluvialis would be a good resource of valuable fatty acids.

关键词： Haematococcus pluvialis astaxanthin esters cyst formation fatty acids GC-MS

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棕囊藻赤潮原因种的分子鉴定和起源分析

海洋学报 2002年第6期24卷 99-103页

作者：陈月琴王宁周惠屈良鹄杨练锋吕颂辉齐雨藻中山大学基因工程教育部重点实验室广东广州510275 国家海洋局南海环境监测中心广东广州510300 暨南大学水生生物研究所广东广州510632

测定了发生于我国东南海域赤潮原因种Phaeocystissp.及相关种类P globosa和P pouchetii18SrDNA序列,并以Phylip35分析软件构建序列距离矩阵和分子系统发育树图.结果表明,该赤潮原因种与球形棕囊藻P globosa18SrDNA序列完全相同,从分子... 详细信息

测定了发生于我国东南海域赤潮原因种Phaeocystissp.及相关种类P globosa和P pouchetii18SrDNA序列,并以Phylip35分析软件构建序列距离矩阵和分子系统发育树图.结果表明,该赤潮原因种与球形棕囊藻P globosa18SrDNA序列完全相同,从分子水平鉴定了该原因种为P globosa.分子系统发育树图表示的P globosa和P pouchetii的分化顺序表明,分布于我国东南海域的P globosa可能是一种本地起源的暖水种.Phaeocystisglobosa不同株系的形态差异,可能是适应不同生活环境的结果.根据分子生物学的数据对有害赤潮藻类进行系统发育分析,并设计用于有害赤潮藻快速鉴定的特异性分子探针,对赤潮的监控和防治具有重要意义.

关键词：分子鉴定起源分析棕囊藻赤潮分子生物学分化顺序

Comparative studies on zooplankton community between the sea surface microlayer and the subsurface microlayer in Daya Bay, china

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Progress in Natural Science:Materials International 2005年第8期15卷 713-719页

作者： YANG Yufeng*(institute of hydrobiology, jinan university, guangzhou 510632, china) institute of hydrobiology Jinan University Guangzhou 510632 China

Characteristics of the zooplankton community inhabiting the sea surface microlayer (SM) and the sub-surface microlayer (SSM) are compared at six sampling stations in Daya Bay, near Shenzhen City of china during 2 cruises in 1999. This is the first study on zooplankton community in the SM in china. Results show that protozoans and nauplii are the most dominant components, accounting for 80.71% (SM) and 89.15% (SSM) of the total zooplankton in the average abundance, respectively. The densities of copepods (adult + copepodid) are higher in the SSM than in the SM. The size-frequency distributions indicate that the frequency of micro-zooplankton (35, n=290) than in the SSM (0.6768, n=306). Enrichment phenomenon of zooplankton is detected in the SM at the sampling stations excluding two stations near nuclear power plants (NPP). The enrichment factor is from 1.516 to 3.364 with the average value of 2.267. The SM zooplankton community structure revealed in the present study is quite different from previous investigations in the Bay. Typical sea water characteristics such as turbidity, biological oxygen demand (BOD), chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP) and chlorophyll a (Chl-a) indicate that the water quality is poorer in the SM than in the SSM.

关键词： zooplankton density sea surface microlayer subsurface microlayer Daya Bay.

灭活菌苗免疫的中华倒刺鲃外周血免疫指标的变化

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水产学报 2010年第4期34卷 626-634页

作者：罗芬张其中冯汉茹朱成科袁娟鉏超西南大学生命科学学院淡水生物生殖与发育教育部重点实验室三峡库区生态环境教育部重点实验室水产科学重庆市市级重点实验室宁德师范高等专科学校生物工程系暨南大学水生生物研究所

以温和气单胞菌灭活菌苗为免疫原,平均体重(100±25)g的健康中华倒刺鲃为实验对象,免疫组腹腔注射0.2mL浓度为1.0×108CFU/mL的免疫原,对照组注射等量灭菌生理盐水,分别在单次注射0、1、2、4、7、14、21、28、35d后随机从两组各取6尾实... 详细信息

以温和气单胞菌灭活菌苗为免疫原,平均体重(100±25)g的健康中华倒刺鲃为实验对象,免疫组腹腔注射0.2mL浓度为1.0×108CFU/mL的免疫原,对照组注射等量灭菌生理盐水,分别在单次注射0、1、2、4、7、14、21、28、35d后随机从两组各取6尾实验鱼,尾静脉采血,测定外周血的血细胞数量、白细胞分类计数、吞噬活性、抗体效价和蛋白质含量等免疫指标的变化,第35天活菌攻毒。结果表明:温和气单胞菌灭活菌苗(F-AS)可诱导中华倒刺鲃红细胞和白细胞数量增加,并引起各种白细胞分类百分比变化,提高吞噬活性和抗体效价,血清中总蛋白及球蛋白含量增加,红细胞也具有免疫功能,灭活茵苗的相对免疫保护力达65.21%。免疫早期(第1周)主要是红细胞、单核细胞和中性粒细胞数量明显增加,吞噬细胞的吞噬活性迅速提高,吞噬百分比和吞噬指数第4天达峰值;随后则是淋巴细胞大量增殖,第21天淋巴细胞、抗体效价及球蛋白达峰值。可见灭活菌苗通过促进中华倒刺鲃血细胞增殖、提高吞噬细胞的吞噬活性、产生特异性抗体等方式提高免疫保护力;免疫早期非特异性细胞免疫起重要作用,之后特异性免疫起主要作用。

关键词：中华倒刺鲃灭活温和气单胞菌菌苗血细胞数量白细胞分类计数吞噬活性抗体效价

Intein-mediated Rapid Purification of Recombinant Human Pituitary Adenylate Cyclase Activating Polypeptide

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 759-766页

作者： Rong-jie YU, An HONG, Yun DAI, and Yuan GAOBio-engineering institute of jinan university, guangzhou 510632, china Bio-engineering institute of jinan university Guangzhou 510632 China

In order to obtain the recombinant human PACAP efficiently by intein-mediated single column purification, a gene encoding human PACAP was synthesized and cloned into Escherichia coli expression vector pKYB. The recomb... 详细信息

关键词： intein recombinant human pituitary adenylate cyclase activating peptide (rhPACAP) purification

A novel cell subset:Interferon-producing killer dendritic cells

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Science china(Life Sciences) 2008年第8期51卷 671-675页

作者： WANG JiongKun & XING FeiYue institute of Tissue Transplantation and Immunology, jinan university, guangzhou 510632, china institute of Tissue Transplantation and Immunology Jinan University Guangzhou China

Recent reports introduce a novel cell subset of DCs with antigenic phenotypes shared by both NK cells and B cells, but without surface markers of pDCs and T cells, appearing to be a chimera of NK cells and DCs, namely interferon-producing killer dendritic cells(IKDCs).IKDCs not only secret type I and type II interferons to recognize and kill tumor cells effectively, but also express MHC-II molecules to present ***, IKDCs are considered as important immunosurveilance cells for tumors, providing a link between innate and adaptive immunity.

关键词： IKDCs cDCs pDCs NK cells

Method of Green’s function of nonlinear vibration of corrugated shallow shells

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Science china(Physics,Mechanics & Astronomy) 2008年第6期51卷 678-686页

作者： YUAN Hong Key Laboratory of Disaster Forecast and Control in Engineering, Ministry of Education of china, institute of Applied Mechanics, jinan university, guangzhou 510632, china Key Laboratory of Disaster Forecast and Control in Engineering Ministry of Education of China Institute of Applied Mechanics Jinan University Guangzhou China

Based on the dynamic equations of nonlinear large deflection of axisymmetric shallow shells of revolution, the nonlinear free vibration and forced vibration of a corrugated shallow shell under concentrated load acting at the center have been investigated. The nonlinear partial differential equations of shallow shell were re-duced to the nonlinear integral-differential equations by using the method of Green’s function. To solve the integral-differential equations, the expansion method was used to obtain Green’s function. Then the integral-differential equations were reduced to the form with a degenerate core by expanding Green’s function as a series of characteristic function. Therefore, the integral-differential equations be-came nonlinear ordinary differential equations with regard to time. The ampli-tude-frequency relation, with respect to the natural frequency of the lowest order and the amplitude-frequency response under harmonic force, were obtained by considering single mode vibration. As a numerical example, nonlinear free and forced vibration phenomena of shallow spherical shells with sinusoidal corrugation were studied. The obtained solutions are available for reference to the design of corrugated shells.

关键词： corrugated shells spherical shells Green’s function integral equation nonlinear vibration

GREEN QUASIFUNCTION METHOD FOR FREE VIBRATION OF SIMPLY-SUPPORTED TRAPEZOIDAL SHALLOW SPHERICAL SHELL ON WINKLER FOUNDATION

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Acta Mechanica Solida Sinica 2010年第4期23卷 370-376页

作者： Shanqing Li Hong Yuan (MOE Key Laboratory of Disaster Forecast and Control in Engineering, institute of Applied Mechanics, jinan university, guangzhou 510632, china) MOE Key Laboratory of Disaster Forecast and Control in Engineering Institute of Applied Mechanics Jinan University Guangzhou China

The idea of Green quasifunction method is clarified in detail by considering a free vibration problem of simply-supported trapezoidal shallow spherical shell on Winkler foundation.A Green quasifunction is established by using the fundamental solution and boundary equation of the *** function satisfies the homogeneous boundary condition of the *** mode shape differential equation of the free vibration problem of simply-supported trapezoidal shallow spherical shell on Winkler foundation is reduced to two simultaneous Fredholm integral equations of the second kind by Green *** are multiple choices for the normalized boundary *** on a chosen normalized boundary equation, the irregularity of the kernel of integral equations is ***, natural frequency is obtained by the condition that there exists a nontrivial solution in the numerically discrete algebraic equations derived from the integral *** results show high accuracy of the Green quasifunction method.

关键词： Green function integral equation shallow spherical shell free vibration Winkler foundation

New species and new records of diatoms from Xiamen Harbour

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Acta Oceanologica Sinica 1992年第3期11卷 449-451页

作者： Li Yaqin,Cheng Zhaodi and Jin Dexiang (Chin T. G. ) institute of hydrobiology,jinan university,guangzhou 510632,china Department of Biology,Xiamen university,Xiamen 361005,china

In planktonic diatom samples collected from Xiamen Harbour, Xiamen,Fujian,china from January 1983 to December 1984, two species and varieties are new to science and three species are recorded for the first time in china. New species is Tropidoneis constricta Li,Cheng et Chin sp. nov. ,new variety is Fragilaria longissima v. protenta Li,Cheng et Chin var. nov. ,new recorded species are FragUaria heidenii *** Cleve and Thalassiosira punctigera Hasle.

关键词： New species and new records of diatoms from Xiamen Harbour

A Dynamical Model of Biological Population Under the Variation of Resource and Its Numerical Analysis

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生物数学学报 1997年第2期12卷 121-128页

作者： Han Boping Qi Yuzao Huang Yihua(institute of Aquatic Ecoscience,jinan university,guangzhou 510632,china) 靖安大学水生生物所广东广州

On the basis of evaluating the logistic model,a model relating to the resource consumption and the resouce provision is developed to investigate the dynamic behavior of population under the variation of resource,its difference equation with discrete time is *** resource dynamics,the population dynamics and its sensitivity to model parameters are analyzed by numerical computation,in *** results show that the resource availability,which depends on the interaction between organisms and their environment,takes a key role in the dynamics of *** there are several limiting resouces,the determination of the minimal limiting resource is also the result of inert action between the biological population and its environment.

关键词： Model population dynamics resource availability