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Chinese Science Bulletin 2000年第22期45卷 2084-2088页

作者： HUANG Yaoxionginstitute of biomedical engineering, ji’nan university, guangzhou 510632. china institute of biomedical Enginecring Ji’nan University Guangzhou China

The principle of the technique of microscope laser light scattering spectroscopy, its development and applications to the studies on single intact cells, including our work on non-invasive in situ real time measurements on single intact erythrocyte simultaneously at the levels of cellular, sub-cellular and intracellular molecules, are introduced.

关键词： microscope laser light scattering single intact cell non-invasive in situ measurement.

Intein-mediated Rapid Purification of Recombinant Human Pituitary Adenylate Cyclase Activating Polypeptide

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 759-766页

作者： Rong-jie YU, An HONG, Yun DAI, and Yuan GAOBio-engineering institute of jinan university, guangzhou 510632. china Bio-engineering institute of jinan university Guangzhou 510632 China

In order to obtain the recombinant human PACAP efficiently by intein-mediated single column purification, a gene encoding human PACAP was synthesized and cloned into Escherichia coli expression vector pKYB. The recomb... 详细信息

关键词： intein recombinant human pituitary adenylate cyclase activating peptide (rhPACAP) purification

Method of Green’s function of nonlinear vibration of corrugated shallow shells

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Science china(Physics,Mechanics & Astronomy) 2008年第6期51卷 678-686页

作者： YUAN Hong Key Laboratory of Disaster Forecast and Control in engineering, Ministry of Education of china, institute of Applied Mechanics, jinan university, guangzhou 510632. china 1. Key Laboratory of Disaster Forecast and Control in engineering Ministry of Education of China Institute of Applied Mechanics Jinan University Guangzhou 510632 China

Based on the dynamic equations of nonlinear large deflection of axisymmetric shallow shells of revolution, the nonlinear free vibration and forced vibration of a corrugated shallow shell under concentrated load acting at the center have been investigated. The nonlinear partial differential equations of shallow shell were re-duced to the nonlinear integral-differential equations by using the method of Green’s function. To solve the integral-differential equations, the expansion method was used to obtain Green’s function. Then the integral-differential equations were reduced to the form with a degenerate core by expanding Green’s function as a series of characteristic function. Therefore, the integral-differential equations be-came nonlinear ordinary differential equations with regard to time. The ampli-tude-frequency relation, with respect to the natural frequency of the lowest order and the amplitude-frequency response under harmonic force, were obtained by considering single mode vibration. As a numerical example, nonlinear free and forced vibration phenomena of shallow spherical shells with sinusoidal corrugation were studied. The obtained solutions are available for reference to the design of corrugated shells.

关键词： corrugated shells spherical shells Green’s function integral equation nonlinear vibration

RAZUMIKHIN-TYPE THEOREMS FOR ASYMPTOTIC STABILITY OF IMPULSIVE STOCHASTIC FUNCTIONAL DIFFERENTIAL SYSTEMS

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Journal of Systems Science and Systems engineering 2010年第1期19卷 72-84页

作者： Pei CHENG~1 Feiqi DENG~2 Xisheng DAI~3 Systems engineering institute,South china university of Technology,guangzhou 510640,china Systems engineering institute South China University of Technology Guangzhou China

In this paper, we investigate the pth moment uniformly asymptotic stability of impulsive stochastic ftmctional differential systems by extending some Razumikhin-type theorems. Based on the Lyapunov functions and Razumikhin techniques, some criteria are established and their applications to impulsive stochastic delay systems are proposed. An illustrative example shows the effectiveness of our results.

关键词： Stochastic functional differential systems Impulse Razumiltial theorems Asymptotic stability

Effects of Citral on Aspergillus flavus Spores by Quasi-elastic Light Scattering and Multiplex Microanalysis Techniques

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 277-283页

作者： Man LUO1,2, Li-Ke jiANG2, Yao-Xiong HUANG3, Ming XIAO4, Bo LI4, and Guo-Lin ZOU1* 1College of Life Sciences, Wuhan university, Wuhan 430072, china 2College of Life Sciences, Anhui Agricultural university, Hefei 230036, china 3 institute of biomedical engineering, ji’nan university, guangzhou 510632. china 4Ministry of Education Key Laboratory for Biodiversity Science and Ecological engineering, the institute of Biodiversity Science, Fudan university, Shanghai 200433, china College of Life Sciences Wuhan UniversityWuhan 430072 China College of Life Sciences Anhui Agricultural UniversityHefei 230036 China institute of biomedical engineering Ji'nan UniversityGuangzhou 510632 China Ministry of Education Key Laboratory for Biodiversity Science and Ecological engineering the Institute of Biodiversity Science Fudan UniversityShanghai 200433 China

Citral refined from Litsea cubeba oil has been found to have a strong influence on fungi, especially Aspergillus flavus. Multiplex microanalysis and quasi-elastic light scattering techniques were applied to study the effects of citral on Aspergillus flavus spores from the levels of membrane, organelle and intracellular macromolecule. It was found that citral injured the wall and the membrane of A. flavus spore, resulting in decrease of its elasticity. After entering the cell, citral not only influenced the genetic expression of mitochondrion reduplication and its morphology, but also changed the aggregation of protein-like macromolecules. As a result, cells, organelles and macromolecules lost their normal structures and functions, eventually leading to the loss of germination ability of A. flavus spores. Since Litsea cubeba oil as food additive and antifungal agent is safe and less poisonous, it is important to elucidate the inhibitory mechanisms of Litsea cubeba oil on the germination ability of A. flavus spore.

关键词： citral Aspergillus flavus spore multiplex microanalysis techniques quasi-elastic light scattering technique

GREEN QUASIFUNCTION METHOD FOR FREE VIBRATION OF SIMPLY-SUPPORTED TRAPEZOIDAL SHALLOW SPHERICAL SHELL ON WINKLER FOUNDATION

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Acta Mechanica Solida Sinica 2010年第4期23卷 370-376页

作者： Shanqing Li Hong Yuan (MOE Key Laboratory of Disaster Forecast and Control in engineering, institute of Applied Mechanics, jinan university, guangzhou 510632. china) [a] MOE Key Laboratory of Disaster Forecast Control in engineering Institute of Applied Mechanics Jinan University Guangzhou 510632 China

The idea of Green quasifunction method is clarified in detail by considering a free vibration problem of simply-supported trapezoidal shallow spherical shell on Winkler foundation.A Green quasifunction is established by using the fundamental solution and boundary equation of the *** function satisfies the homogeneous boundary condition of the *** mode shape differential equation of the free vibration problem of simply-supported trapezoidal shallow spherical shell on Winkler foundation is reduced to two simultaneous Fredholm integral equations of the second kind by Green *** are multiple choices for the normalized boundary *** on a chosen normalized boundary equation, the irregularity of the kernel of integral equations is ***, natural frequency is obtained by the condition that there exists a nontrivial solution in the numerically discrete algebraic equations derived from the integral *** results show high accuracy of the Green quasifunction method.

关键词： Green function integral equation shallow spherical shell free vibration Winkler foundation

SYNTHESIS AND OPTICAL PROPERTIES OF A NOVEL ORGANIC/INORGANIC HYBRID NONLINEAR OPTICAL POLYMER VIA SOL-GEL PROCESS

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Chinese Journal of Polymer Science 2001年第4期19卷 421-427页

作者： Hong-xia Xi Zhong Li Zhao-xi Liang The institute of Chemical engineering South china university guangzhou 510640 china The institute of Polymer Science, Zhongshan university guangzhou 510275, china S china Univ Inst Chem Engn Canton 510640 Peoples R China Zhongshan Univ Inst Polymer Sci Canton 510275 Peoples R China

A new organic/inorganic hybrid nonlinear optical (NLO) material was developed by the sol-gel process of an alkoxysilane dye with tetraethoxysilane. A NLO moiety based on 4-nitro-4 ' -hydroxy azobenzene was covalently bonded to the triethoxysilane derivative, i.e, gamma -isocyanatopropyl triethoxysilane. The preparation process and properties of the sol-gel derived NLO polymer were studied and characterized by SEM, FTIR,H-1-NMR, UV-Vis, DSC and second harmonic generation (SHG) measurement. The results indicated that the chemical bonding of the chromophores to the inorganic SiO2 networks induces low dipole alignment relaxation and preferable orientational stability. The SHG measurements also showed that the bonded polymer film containing 75 wt% of the akoxysilane dye has a high electro-optic coefficient (r(33)) of 7.1 pm/V at 1.1 mum wavelength, and exhibit good SHG stability, the r(33) values can maintain about 92.7% of its initial value at room temperature for 90 days, and can maintain about 59.3% at 100 degreesC for 300 min.

关键词： sol-gel process nonlinear optical material alkoxysilane dye dipole alignment relaxation chromophore

Reflection of plane electromagnetic waves from the surface of a perfect conductor moving in an arbitrary direction

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Chinese Science Bulletin 2000年第17期45卷 1564-1569页

作者： HE Qicai & HUANG Yaoxiong1. Department of Physics, Sun Yat-sen university of Medical Sciences, guangzhou 510089, china 2. institute of biomedical engineering, ji’nan university, guangzhou 510632. china Correspondence should be addressed to Huang Yaoxiong Department of Physics Sun Yat-sen University of Medical Sciences Guangzhou China institute of biomedical engineering Ji’nan University Guangzhou China

The reflection of plane electromagnetic waves (TE wave and TM wave) from a perfect conductor which moves in an arbitrary direction is investigated. Based on Maxwell’s equations and the boundary conditions for moving boundary, the relation between the field vectors of reflected and incident waves, and the reflection coefficient are derived. The energy balance between incident and reflected waves, the force exerted by electromagnetic waves to the moving conductor are also discussed and some new conclusions are suggested for notice.

关键词： plane electromagnetic wave moving conductor reflection coefficient radiation pressure.

Parameter identification theory of a complex model based on global optimization method

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Science china(Physics,Mechanics & Astronomy) 2008年第11期51卷 1722-1732页

作者： QU jie1, jiN QuanLin2 & XU BingYe3 1 College of Automotive engineering, South china university of Technology, guangzhou 510640, china 2 Beijing Research institute of Mechanical and Electrical Technology, Beijing 100083, china 3 Department of engineering Mechanics, Tsinghua university, Beijing 100084, china 1. College of Automotive engineering South China University of Technology Guangzhou 510640 China 2. Beijing Research institute of Mechanical and Electrical Technology Beijing 100083 China 3. Department of engineering Mechanics Tsinghua University Beijing 100084 China

With the development of computer technology and numerical simulation technology, computer aided engineering (CAE) technology has been widely applied to many fields. One of the main obstacles, which hinder the further application of CAE technology, is how to successfully identify the parameters of the selected model. An elementary framework for parameter identification of a complex model is provided in this paper. The framework includes the construction of objective function, the design of the optimization method and the evaluation of the identified results, etc. The parameter identification process is described in this framework, taking the parameter identification of the superplastic constitutive model considering grain growth for Ti-6Al-4V at 927°C as an example. The objective function is the weighted quadratic sums of the difference between the experimental and computational data for the stress-strain relationship and the grain growth relationship; the designed optimization method is a hybrid global optimization method, which is based on the feature of the objective function and incorporates the strengths of genetic algorithm (GA), the Levenberg-Marquardt algorithm and the augmented Gauss-Newton algorithm. The reliability evaluation of parameter identification result is made through the comparison between the calculated and experimental results and between the theoretical values of the parameters and the identified ones.

关键词： constitutive model parameter identification theory inverse analysis global optimization

High-throughput and simultaneous inertial separation of tumor cells and clusters from malignant effusions using spiral-contraction-expansion channels

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Microsystems & nanoengineering 2024年第2期10卷 91-99页

作者： Zhixian Zhu Hui Ren Dan Wu Zhonghua Ni nan Xiang School of Mechanical engineering and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical InstrumentsSoutheast University211189NanjingChina School of Mechanical Technology Wuxi Institute of TechnologyNo.1600 Gaolang West Road214129WuxiChina Department of Oncology Jiangyin People’s Hospital214400JiangyinChina

Tumor cell clusters are regarded as critical factors in cancer pathophysiology,and increasing evidence of their higher treatment resistance and metastasis compared to single tumor cells has been ***,existing cell separation methods that are designed for single tumor cells cannot be used to simultaneously purify tumor cell *** address this problem,we demonstrated a microfluidic approach for the high-throughput,continuous-flow ternary separation of single tumor cells,tumor cell clusters,and WBCs from clinical pleural or abdominal effusions by coupling slanted spiral channels and periodic contraction-expansion *** first systematically explored the influence of particle size and flow rate on particle *** separation performance indicated that 94.0%of WBCs were removed and more than 97%of MDA-MB-231 tumor cells were recovered at a high flow rate of 3500µL/***,more than 90%of tumor cell clusters were effectively preserved after ***,we successfully applied our device for the ternary separation of single tumor cells,tumor cell clusters,and WBCs from different malignant effusions collected from patients with metastatic ***,our spiral-contraction-expansion device has potential as a sample pretreatment tool for the cytological diagnosis of malignant effusions.

关键词： separation contraction removed