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Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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Science China(Life Sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3,ZHOU YuLei 4.5 ,SONG LinLin 6 ,ZHANG Yan 3 &ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China 2 Department of Chemistry and Chemical engineering,Hu{4. institute of engineering,Xiangtan 4.1104.China 3 College of Life Science,Capital Normal university,Beijing 100037,China 4.College of Life Science,Zhongkai university of Agriculture and Technology,guangzhou 510225,China 5 College of Grassland Science,Gansu Agricultural university,Lanzhou 730070,China 6 Department of Biology,He{4. institute of Science and Technology,Xinxiang 4.3003,China Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing China Department of Chemistry and Chemical engineering Hunan Institute of Engineering Xiangtan China College of Life Science Capital Normal University Beijing China College of Life Science Zhongkai University of Agriculture and Technology Guangzhou China College of Grassland Science Gansu Agricultural University Lanzhou China Department of Biology Henan Institute of Science and Technology Xinxiang China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC74.H/sacB,which contain about 165000 and 236000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384.well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC74.H/sacB were stored individually in fourteen 384.well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

Biological Feature of Collagen-Chitosan Membrane with Basic Fibroblast Growth Factor for the Culture of Human Fibroblast

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Chinese Journal of biomedical engineering(English Edition) 2003年第4期12卷 169-173页

作者： LI Qin-hua,ZHONG Yuinstitute of biomedical engineering,Center for reproductive immunology research,ji{4. university, guangzhou 510632, China

The effect of collagen-chitosan membrane with different proportionate collagen and bFGF were investigated for culture human fibroblast. The optimum weight ratio of collagen/chitoson and bFGF were selected. Using culture human fibroblast technologies and cytotoxicity evaluated in vitro, Cell morphology was observed. Experimental results show that collagen-chitosan with bFGF promoted human fibroblast adhesion and supported cell proliferation for a long time. Furthermore collagen-chitosan membrane obviously degrade after 18d when human fibroblast was exhibited fusion spreading, compacting and stabilize. Cytotoxic to human fibroblast was revealed very low . Collagen- chitosan with bFGF should be useful as a tissue engineering biomaterial scaffold for cell culture.

关键词： Collagen-chitosan bFGF Human fibroblast Cell culture

Enhanced Compression of Fundamental Solitons in Dispersion Decreasing Fibers with Negative Third-order Dispersion Ⅱ. Effects of Fiber Characteristics and Initial Soliton Width

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Chinese Journal of Lasers 2001年第6期10卷 66-72页

作者： CAO Wenhua (Department of Electronic \%&\% Information engineering, Wuyi university, jiangmen 529020, China) GUO Qi LIU Songhao (institute of Quantum Electronics, South China Normal university, {4. 510631, China) 五邑大学电子与信息工程系广东江门 529020 华南师范大学量子电子学所广东广州 510631

Previous work have shown that the combined effects of negative third-order dispersion (TOD) and Raman self-scattering (RSS) can significantly enhance soliton compression in dispersion decreasing fibers (DDFs). In this paper, the effects of the negative TOD coefficient and the effective amplification of DDF′s on the performance of soliton compression are investigated. It is shown that for a given initial soliton width and a given effective amplification, there exists an optimum value of the negative TOD coefficient of the DDF at which the enhancement in soliton compression is maximum. It is also shown that the compression enhancement saturates when the effective amplification exceeds a certain value, which has been explained as a compromise between the higher-order effects induced increase of the ratio of input to output group-velocity dispersion coefficients of the DDF and the TOD induced non-adiabatic compression characteristics. The dependence of the compression enhancement on the initial soliton width have also be studied and the scheme is found works well for solitons with initial widths less than 3 ps.

关键词： fundamental solitons pulse compression dispersion decreasing fibers third-order dispersion Raman self-scattering

Eliminating H_(2)O/HF and regulating interphase with bifunctional tolylene-2,4.diisocyanate(TDI)additive for long life Li-ion battery

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Journal of Energy Chemistry 2024年第8期95卷 519-528,I0011页

作者： Xueyi Zeng Xiang Gao Peiqi Zhou Haijia Li Xin He Weizhen Fan Chaojun Fan Tianxiang Yang Zhen Ma Xiaoyang Zhao Junmin nan School of Chemistry South China Normal UniversityGuangzhou 510006GuangdongChina guangzhou Tinci Materials Technology Co.Ltd. Guangzhou 510760GuangdongChina School of Environmental engineering and Chemistry Luoyang Institute of Science and TechnologyLuoyang 471023HenanChina School of Geomatic and Environmental engineering Henan Polytechnic InstituteNanyang 473000HenanChina

Lithium-ion batteries(LIBs)featuring a Ni-rich cathode exhibit increased specific capacity,but the establishment of a stable interphase through the implementation of a functional electrolyte strategy remains *** when the battery is operated under high temperature,the trace water present in the electrolyte will accelerate the hydrolysis of the electrolyte and the resulting HF will further erode the *** order to enhance the long-term cycling performance of graphite/LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)(NCM811)LIBs,herein,Tolylene-2,4.diisocyanate(TDI)additive containing lone-pair electrons is employed to formulate a novel bifunctional electrolyte aimed at eliminating H_(2)O/HF generated at elevated *** 1000 cycles at 25℃,the battery incorporating the TDI-containing electrolyte exhibits an impressive capacity retention of 94.at 1 *** contrast,the battery utilizing the blank electrolyte has a lower capacity retention of only 78%.Furthermore,after undergoing 550 cycles at 1 C under4.℃,the inclusion of TDI results in a notable enhancement of capacity,increasing it from 68%to 80%.This indicates TDI has a favorable influence on the cycling performance of LIBs,especially at elevated *** analysis of the film formation mechanism suggests that the lone pair of electrons of the isocyanate group in TDI play a crucial role in inhibiting the generation of H_(2)O and HF,which leads to the formation of a thin and dense *** existence of this interphase is thought to substantially enhance the cycling performance of the *** work not only improves the performance of graphite/NCM811 batteries at room temperature and high temperature by eliminating H_(2)O/HF but also presents a novel strategy for advancing functional electrolyte development.

关键词： Graphite/LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)battery Tolylene-2 4.diisocyanate Long-cycling performance H_(2)O/HF eliminated additive

Regulating the electronic structure of Ir single atoms by ZrO_(2)nanoparticles for enhanced catalytic oxidation of formaldehyde at room temperature

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Chinese Chemical Letters 2024年第7期35卷 200-205页

作者： Shiqi Peng Yongfang Rao Tan Li Yufei Zhang Jun-ji Cao Shuncheng Lee Yu Huang Key Laboratory of Aerosol Chemistry&Physics State Key Laboratory of Loess and Quaternary Geology(SKLLQG)Institute of Earth EnvironmentChinese Academy of Sciences(CAS)Xi’an 710061China School of Human Settlements and Civil engineering Xi’an Jiaotong UniversityXi’an 710049China Department of Environmental Science and engineering Xi’an Jiaotong UniversityXi’an 710049China Faculty of Chemical engineering Kunming University of Science and TechnologyKunming 650500China School of Environment and Energy South China University of TechnologyGuangzhou 51006China School of Environmental and Municipal engineering Xi’an University of Architecture and TechnologyXi’an 710055China institute of Atmospheric Physics Chinese Academy of SciencesBeijing 100190China Department of Civil and Environmental engineering The Hong Kong Polytechnic UniversityHong KongChina CAS Center for Excellence in Quaternary Science and Global Change Xi’an 710061China

Developing low-loading single-atom catalysts with superior catalytic activity and selectivity in formaldehyde(HCHO)oxidation at room temperature remains ***,ZrO_(2)nanoparticles coupled low-loading Ir single atoms in N-doped carbon(Ir_(1)-N-C/ZrO_(2))was *** optimal Ir_(1)-N-C/ZrO_(2)with 0.25 wt%Ir loading delivers the high HCHO removal and conversion efficiency(>95%)at 20℃,which is higher than that over Ir_(1)-N-C with the same Ir *** specific rate can reach 1285.6 mmol gIr^(-1)h^(-1),surpassing the Ir based catalysts reported to *** functional theory calculation results and electron spin resonance spectra indicate that the introduction of Zr O_(2)nanoparticles modulate the electronic structure of the Ir single atoms,promoting O_(2)activation to·O_(2)^(–).Moreover,the Ir-C-Zr channel is favorable for the dissociation of·O_(2)^(–)to active oxygen atom(*O),and further accelerates the transformation of HCHO and intermediates(dioxymethylene and formates)to CO_(2)and H_(2)*** work provides a facile strategy to design low-loading single-atom catalysts with high catalytic activity toward HCHO oxidation.

关键词： ZrO_(2)nanoparticles Ir single atoms Electronic structure Formaldehyde Catalytic oxidation

OBSERVATION ON DEFECTS IN POLYCRYSTALLINE SILICON THIN FILMS

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Acta Metallurgica Sinica(English Letters) 2005年第3期18卷 295-299页

作者： Y.F. Hu1), H. Shen2), L. Wang3), Z.C. Liang3), Z.Y. Liu1) and L.S. Wen1) 1) College of Mechanical engineering, South China university of Technology, Guan gzhou 51064., China 2) The School of Physics and engineering, Zhongshan university,{4. 510275 , China 3){4. institute of Energy Conversion, The Chinese Academy of Sciences, G uangzhou 51064., China College of Mechanical engineering South China University of Technology Guangzhou 510640 China School of Physics and engineering Zhongshan University Guangzhou 510275 China guangzhou institute of Energy Conversion Chinese Academy of Sciences Guangzhou 510640 China

Polycrystalline silicon thin films were prepared by RTCVD (rapid thermal chemica l vapor deposition) method on several substrates such as SSP (silicon sheet from powder) ribbon, poly-Si wafer and mono-Si wafer. Intra-granular defects such as stacking faults, twins and microstructure defects were investigated on thin fil ms by scan electron microscopy (SEM) technique.

关键词： poly-Si thin film intra-granular defects surface cross-section

Brain age estimation:premise,promise,and problems

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Neural Regeneration Research 2025年第8期20卷 2313-2314页

作者： Jarrad Perron ji Hyun Ko Graduate Program in biomedical engineering Price Faculty of EngineeringUniversity of ManitobaWinnipegMBCanada PrairieNeuro Research Centre Kleysen Institute for Advanced MedicineHealth Sciences CentreWinnipegMBCanada Department of Human Anatomy and Cell Science Rady Faculty of Health SciencesUniversity of ManitobaWinnipegMBCanada

Premise:The com bined effects of modern healthcare practices which prolong lifespan and declining birthrates have created unprecedented changes in age demographics worldwide that are especially pronounced in Japan,South Korea,Europe,and North *** old age is the most significant predictor of dementia,global healthcare systems must rise to the challenge of providing care for those with neurodegenerative disorders.

关键词： estimation providing birth

SOLUTION CHEMISTRY OF ELECTROKINETIC BEHAVIOR OF CARBONATE MINERALS

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中国有色金属学会会刊：英文版 1995年第4期5卷 26-30页

作者： Hu Yuehua Luo Lin Qiu guangzhou(Department of Mineral engineering,Central South university of Technology,Changsha 4.0083) Wang Dianzuo(Beijing General Research {4. for Nonferrous Metals,Beijing 100088) Central South Univ of Technology Changsha China

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关键词： carbonate type minerals electrokinetic behavior solution chemistry

A review of sources, pathways, and toxic effects of human exposure to benzophenone ultraviolet light filters

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Eco-Environment & Health 2024年第1期3卷 30-44页

作者： Ya-nan Yao You Wang Hengling Zhang Yanxia Gao Tao Zhang Kurunthachalam Kannan School of Environmental Science and engineering Sun Yat-Sen UniversityGuangzhou 510275China Wadsworth Center New York State Department of HealthAlbanyNew YorkNY 12237USA

Benzophenone ultraviolet light filters(BPs)are high-production-volume chemicals extensively used in personal care products,leading to widespread human *** their estrogenic properties,the potential health risks associated with exposure to BPs have become a public health *** review aims to summarize sources and pathways of exposure to BPs and associated health *** exposure,primarily through the use of sunscreens,constitutes a major pathway for BP *** a recommended application rate,dermal exposure of BP-3 via the application of sunscreens may reach or exceed the suggested reference *** exposure pathways to BPs,such as drinking water,seafood,and packaged foods,contribute minimal to the overall *** is a minor pathway of exposure;however,its contribution cannot be *** exposure to BPs is an order of magnitude higher in North America than in Asia and *** conducted on laboratory animals and cells have consistently demonstrated the toxic effects of BP *** are estrogenic and elicit reproductive and developmental ***,neurotoxicity,hepatotoxicity,nephrotoxicity,and carcinogenicity have been reported from chronic BP *** addition to animal and cell studies,epidemiological investigations have identified associations between BPs and couples'fecundity and other reproductive disorders,as well as adverse birth *** studies are urgently needed to understand the risks posed by BPs on human health.

关键词： Benzophenone ultraviolet light filters Human exposure BP-3 Health effects Risk assessment