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Chinese Science Bulletin 2000年第22期45卷 2084-2088页

作者： HUANG Yaoxionginstitute of biomedical engineering, ji’nan university,{4., China institute of biomedical Enginecring Ji’nan University Guangzhou China

The principle of the technique of microscope laser light scattering spectroscopy, its development and applications to the studies on single intact cells, including our work on non-invasive in situ real time measurements on single intact erythrocyte simultaneously at the levels of cellular, sub-cellular and intracellular molecules, are introduced.

关键词： microscope laser light scattering single intact cell non-invasive in situ measurement.

Intein-mediated Rapid Purification of Recombinant Human Pituitary Adenylate Cyclase Activating Polypeptide

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 759-766页

作者： Rong-jie YU, An HONG, Yun DAI, and Yuan GAOBio-engineering institute of ji{4. university, guangzhou 510632, China Bio-engineering institute of jinan {4.guangzhou {4. China

In order to obtain the recombinant human PACAP efficiently by intein-mediated single column purification, a gene encoding human PACAP was synthesized and cloned into Escherichia coli expression vector pKYB. The recomb... 详细信息

关键词： intein recombinant human pituitary adenylate cyclase activating peptide (rhPACAP) purification

Effects of Citral on Aspergillus flavus Spores by Quasi-elastic Light Scattering and Multiplex Microanalysis Techniques

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 277-283页

作者： Man LUO1,2, Li-Ke jiANG2, Yao-Xiong HUANG3, Ming XIAO4. Bo LI4. and Guo-Lin ZOU1* 1College of Life Sciences, Wuhan university, Wuhan 4.0072, China 2College of Life Sciences, Anhui Agricultural university, Hefei 230036, China 3 institute of biomedical engineering,{4., guangzhou 510632, China 4.inistry of Education Key Laboratory for Biodiversity Science and Ecological engineering, the institute of Biodiversity Science, Fudan university, Shanghai 2004.3, China College of Life Sciences Wuhan UniversityWuhan 430072 China College of Life Sciences Anhui Agricultural UniversityHefei 230036 China institute of biomedical engineering Ji'nan UniversityGuangzhou 510632 China Ministry of Education Key Laboratory for Biodiversity Science and Ecological engineering the Institute of Biodiversity Science Fudan UniversityShanghai 200433 China

Citral refined from Litsea cubeba oil has been found to have a strong influence on fungi, especially Aspergillus flavus. Multiplex microanalysis and quasi-elastic light scattering techniques were applied to study the effects of citral on Aspergillus flavus spores from the levels of membrane, organelle and intracellular macromolecule. It was found that citral injured the wall and the membrane of A. flavus spore, resulting in decrease of its elasticity. After entering the cell, citral not only influenced the genetic expression of mitochondrion reduplication and its morphology, but also changed the aggregation of protein-like macromolecules. As a result, cells, organelles and macromolecules lost their normal structures and functions, eventually leading to the loss of germination ability of A. flavus spores. Since Litsea cubeba oil as food additive and antifungal agent is safe and less poisonous, it is important to elucidate the inhibitory mechanisms of Litsea cubeba oil on the germination ability of A. flavus spore.

关键词： citral Aspergillus flavus spore multiplex microanalysis techniques quasi-elastic light scattering technique

Trend of Developing Aqueous Liquid and Gel Electrolytes for Sustainable,Safe,and High‑Performance Li‑Ion Batteries

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nano-Micro Letters 2024年第1期16卷 17-34页

作者： Donghwan ji Jaeyun Kim School of Chemical engineering Sungkyunkwan University(SKKU)Suwon 16419Republic of Korea Department of Health Sciences and Technology Samsung Advanced Institute for Health Sciences&Technology(SAIHST)Sungkyunkwan University(SKKU)Suwon 16419Republic of Korea biomedical institute for Convergence at SKKU(BICS) Sungkyunkwan University(SKKU)Suwon 16419Republic of Korea institute of Quantum Biophysics(IQB) Sungkyunkwan University(SKKU)Suwon 16419Republic of Korea Present Address:Department of nanoengineering University of California San DiegoLa JollaSan DiegoCA 92093USA

Current lithium-ion batteries(LIBs)rely on organic liquid electrolytes that pose significant risks due to their flammability and *** potential for environmental pollution and explosions resulting from battery damage or fracture is a critical ***-based(aqueous)electrolytes have been receiving attention as an alternative to organic ***,a narrow electrochemicalstability window,water decomposition,and the consequent low battery operating voltage and energy density hinder the practical use of aqueous ***,developing novel aqueous electrolytes for sustainable,safe,high-performance LIBs remains *** Review first commences by summarizing the roles and requirements of electrolytes–separators and then delineates the progression of aqueous electrolytes for LIBs,encompassing aqueous liquid and gel electrolyte development trends along with detailed principles of the *** aqueous electrolytes are progressed based on strategies using superconcentrated salts,concentrated diluents,polymer additives,polymer networks,and artificial passivation layers,which are used for suppressing water decomposition and widening the electrochemical stability window of water of the *** addition,this Review discusses potential strategies for the implementation of aqueous Li-metal batteries with improved electrolyte–electrode interfaces.A comprehensive understanding of each strategy in the aqueous system will assist in the design of an aqueous electrolyte and the development of sustainable and safe high-performance batteries.

关键词： Lithium-ion battery(LIB) Aqueous electrolyte Gel electrolyte Electrochemical stability window Li dendrite

Method of Green’s function of nonlinear vibration of corrugated shallow shells

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Science China(Physics,Mechanics & Astronomy) 2008年第6期51卷 678-686页

作者： YUAN Hong Key Laboratory of Disaster Forecast and Control in engineering, Ministry of Education of China, institute of Applied Mechanics, jinan {4., guangzhou 510632, China Key Laboratory of Disaster Forecast and Control in engineering Ministry of Education of China Institute of Applied Mechanics Jinan University Guangzhou China

Based on the dynamic equations of nonlinear large deflection of axisymmetric shallow shells of revolution, the nonlinear free vibration and forced vibration of a corrugated shallow shell under concentrated load acting at the center have been investigated. The nonlinear partial differential equations of shallow shell were re-duced to the nonlinear integral-differential equations by using the method of Green’s function. To solve the integral-differential equations, the expansion method was used to obtain Green’s function. Then the integral-differential equations were reduced to the form with a degenerate core by expanding Green’s function as a series of characteristic function. Therefore, the integral-differential equations be-came nonlinear ordinary differential equations with regard to time. The ampli-tude-frequency relation, with respect to the natural frequency of the lowest order and the amplitude-frequency response under harmonic force, were obtained by considering single mode vibration. As a numerical example, nonlinear free and forced vibration phenomena of shallow spherical shells with sinusoidal corrugation were studied. The obtained solutions are available for reference to the design of corrugated shells.

关键词： corrugated shells spherical shells Green’s function integral equation nonlinear vibration

GREEN QUASIFUNCTION METHOD FOR FREE VIBRATION OF SIMPLY-SUPPORTED TRAPEZOIDAL SHALLOW SPHERICAL SHELL ON WINKLER FOUNDATION

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Acta Mechanica Solida Sinica 2010年第4期23卷 370-376页

作者： Shanqing Li Hong Yuan (MOE Key Laboratory of Disaster Forecast and Control in engineering, institute of Applied Mechanics, jinan {4., guangzhou 510632, China) MOE Key Laboratory of Disaster Forecast and Control in engineering Institute of Applied Mechanics Jinan University Guangzhou China

The idea of Green quasifunction method is clarified in detail by considering a free vibration problem of simply-supported trapezoidal shallow spherical shell on Winkler foundation.A Green quasifunction is established by using the fundamental solution and boundary equation of the *** function satisfies the homogeneous boundary condition of the *** mode shape differential equation of the free vibration problem of simply-supported trapezoidal shallow spherical shell on Winkler foundation is reduced to two simultaneous Fredholm integral equations of the second kind by Green *** are multiple choices for the normalized boundary *** on a chosen normalized boundary equation, the irregularity of the kernel of integral equations is ***, natural frequency is obtained by the condition that there exists a nontrivial solution in the numerically discrete algebraic equations derived from the integral *** results show high accuracy of the Green quasifunction method.

关键词： Green function integral equation shallow spherical shell free vibration Winkler foundation

Non-invasive in Situ Simultaneous Measurement of Multi-parameter Mechanical Properties of Red Blood Cell Membrane

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Acta Biochimica et Biophysica Sinica 2005年第6期37卷 391-395页

作者： jing LI~(1.2) Yao-Xiong HUANG~1 Tao ji~1 Mei TU~1 Xuan MAO~1 Wen-Xin CHEN~1 Guang-Wei CHEN 1 institute of biomedical {4., jinan university, guangzhou 510632, China 2 State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-Sen university, guangzhou 510275, China institute of biomedical engineering Jinan University Guangzhou 510632 China State Key Laboratory for Biocontrol School of Life Science Sun Yat-Sen University Guangzhou 510275 China

The purpose of this study was to develop a new dynamic image analyzing technique that willgive us the ability to measure the viscoelastic parameters of individual living red blood cells non-invasively,insitu and in real *** this technique,the bending modulus Kc,the shear elasticity μ and their ratio ε weremeasured under different temperatures,oxygen partial pressures and osmotic *** results not onlyshow the effects of external conditions on mechanical properties of cell membranes including deformability,flexibility,adhesive ability and plasticity,but also demonstrate that the technique can be used to measure cellmembrane parameters continuously under several physiological and pathological conditions.

关键词： fast image analyzing red blood cell(RBC) cell membrane elastic property

Reflection of plane electromagnetic waves from the surface of a perfect conductor moving in an arbitrary direction

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Chinese Science Bulletin 2000年第17期45卷 1564-1569页

作者： HE Qicai & HUANG Yaoxiong1. Department of Physics, Sun Yat-sen university of Medical Sciences, guangzhou 510089, China 2. institute of biomedical{4., ji’nan university, guangzhou 510632, China Correspondence should be addressed to Huang Yaoxiong Department of Physics Sun Yat-sen University of Medical Sciences Guangzhou China institute of biomedical engineering Ji’nan University Guangzhou China

The reflection of plane electromagnetic waves (TE wave and TM wave) from a perfect conductor which moves in an arbitrary direction is investigated. Based on Maxwell’s equations and the boundary conditions for moving boundary, the relation between the field vectors of reflected and incident waves, and the reflection coefficient are derived. The energy balance between incident and reflected waves, the force exerted by electromagnetic waves to the moving conductor are also discussed and some new conclusions are suggested for notice.

关键词： plane electromagnetic wave moving conductor reflection coefficient radiation pressure.

RAZUMIKHIN-TYPE THEOREMS FOR ASYMPTOTIC STABILITY OF IMPULSIVE STOCHASTIC FUNCTIONAL DIFFERENTIAL SYSTEMS

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Journal of Systems Science and Systems engineering 2010年第1期19卷 72-84页

作者： Pei CHENG~1 Feiqi DENG~2 Xisheng DAI~3 Systems engineering institute,South China university of Technology,guangzhou 51064.,China Systems engineering institute South China University of Technology Guangzhou China

In this paper, we investigate the pth moment uniformly asymptotic stability of impulsive stochastic ftmctional differential systems by extending some Razumikhin-type theorems. Based on the Lyapunov functions and Razumikhin techniques, some criteria are established and their applications to impulsive stochastic delay systems are proposed. An illustrative example shows the effectiveness of our results.

关键词： Stochastic functional differential systems Impulse Razumiltial theorems Asymptotic stability

High-throughput and simultaneous inertial separation of tumor cells and clusters from malignant effusions using spiral-contraction-expansion channels

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Microsystems & nanoengineering 2024年第2期10卷 91-99页

作者： Zhixian Zhu Hui Ren Dan Wu Zhonghua Ni nan Xiang School of Mechanical engineering and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical InstrumentsSoutheast University211189NanjingChina School of Mechanical Technology Wuxi Institute of TechnologyNo.1600 Gaolang West Road214129WuxiChina Department of Oncology Jiangyin People’s Hospital214400JiangyinChina

Tumor cell clusters are regarded as critical factors in cancer pathophysiology,and increasing evidence of their higher treatment resistance and metastasis compared to single tumor cells has been ***,existing cell separation methods that are designed for single tumor cells cannot be used to simultaneously purify tumor cell *** address this problem,we demonstrated a microfluidic approach for the high-throughput,continuous-flow ternary separation of single tumor cells,tumor cell clusters,and WBCs from clinical pleural or abdominal effusions by coupling slanted spiral channels and periodic contraction-expansion *** first systematically explored the influence of particle size and flow rate on particle *** separation performance indicated that 94.0%of WBCs were removed and more than 97%of MDA-MB-231 tumor cells were recovered at a high flow rate of 3500µL/***,more than 90%of tumor cell clusters were effectively preserved after ***,we successfully applied our device for the ternary separation of single tumor cells,tumor cell clusters,and WBCs from different malignant effusions collected from patients with metastatic ***,our spiral-contraction-expansion device has potential as a sample pretreatment tool for the cytological diagnosis of malignant effusions.

关键词： separation contraction removed