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Experimental Study of Rat Beta Islet Cells Cultured under Simulated Microgravity Conditions

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 47-50页

作者： Chun SONG, Xiu-Qing DUAN, Xi LI, Li-Ou HAN, Ping XU, Chun-Fang SONG, and Lian-Hong JIN (Key Laboratory of Cell Transplantation of Ministry of Health, First Affiliated Hospital of harbin medical university, harbin 150001, china department of General Surgery, First Affiliated Hospital of harbin medical university, harbin 150001, china National Key Laboratory of harbin Veterinary Research Institute of Chinese Agriculture Academy, harbin 150001, china Tissue Engineering Research Center, Histology and Embryology Staff Room, harbin medical university, harbin 150086, china ) Key Laboratory of Cell Transplantation of Ministry of Health First Affiliated Hospital of Harbin Medical University Harbin 150001 China department of General Surgery First Affiliated Hospital of Harbin Medical University Harbin 150001 China National Key Laboratory of harbin Veterinary Research Institute of Chinese Agriculture Academy harbin 150001 China Tissue Engineering Research Center Histology and Embryology Staff Room Harbin Medical University Harbin 150086 China

To observe the effects of simulated microgravity on beta islet cell culture, we have compared the survival rates and the insulin levels of the isolated rat islet cells cultured at micro- and normal gravity conditions. The survival rates of the cells cultured were determined by acridine orange-propidium iodide double-staining on day 3, 7 and 14. The morphology of the cells was observed by electron microscopy. Insulin levels were measured by radio immuno assays. Our results show that the cell number cultured under the microgravity condition is significantly higher than that under the routine condition (P<0.01). Some tubular structure shown by transmission electron microscopy, possibly for the transport of nutrients, were formed intercellularly in the microgravity cultured group on day 7. There were also abundant secretion particles and mitochondria in the cytoplasm of the cells. Scanning electron microscopy showed that there were holes formed between each islet, possibly connecting with the nutrient trans

关键词： simulated microgravity islet cell electron microscopy radioimmunoassay

Impaired PI3K/Akt signal pathway and hepatocellular injury in high-fat fed rats

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World Journal of Gastroenterology 2010年第48期16卷 6111-6118页

作者： Ji-Wu Han,department of Gastroenterology,The 4th Hospital of harbin medical university,harbin 150001,Heilongjiang Province,china Xiao-Rong Zhan,Xin-Yu Li,Bing Xia,Yue-Ying Wang,Jing Zhang,department of Endocrinology,First Hospital of harbin medical university,harbin 150001,Heilongjiang Province,china Bao-Xin Li,department of Pharmacology,State Key Laboratory of Biomedicine and Pharmacology,harbin medical university,harbin 150001,Heilongjiang Province,china department of Endocrinology First Hospital of Harbin Medical University Harbin 150001 Heilongjiang Province China.

AIM:To determine whether mitochondrial dysfunction resulting from high-fat diet is related to impairment of the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt,also known as PKB) pathway. METHODS:Rat models of nonalcoholic fatty liver were established by high-fat diet feeding. The expression of total and phosphorylated P13K and Akt proteins in hepatocytes was determined by Western blotting. Degree of fat accumulation in liver was measured by hepatic triglyceride. Mitochondrial number and size were determined using quantitative morphometric analysis under transmission electron microscope. The permeability of the outer mitochondrial membrane was assessed by determining the potential gradient across this ***:After Wistar rats were fed with high-fat diet for 16 wk,their hepatocytes displayed an accumulation of fat (103.1 ± 12.6 vs 421.5 ± 19.7,P 3% vs 83.0% ± 10.9%,P 389.385% ± 18.612% vs 249.121% ± 13.526%,P 3K and Akt proteins in hepatocytes was reduced,as was the expression of the anti-apoptotic protein Bcl-2,while expression of the pro-apoptotic protein caspase-3 was increased. When animals were treated with pharmacological inhibitors of P13K or Akt,instead of high-fat diet,a similar pattern of hepatocellular fat accumulation,mitochondrial impairment,and change in the levels of PI3K,Akt,Bcl-2 was observed. CONCLUSION:High-fat diet appears to inhibit the PI3K/Akt signaling pathway,which may lead to hepa-tocellular injury through activation of the mitochondrial membrane pathway of apoptosis.

关键词： Nonalcoholic fatty liver Phosphatidylinositol 3-kinase/protein kinase B signaling pathway Mitochondria B-cell lymphoma gene 2 Caspase-3

Advances in gene therapy of liver cirrhosis: a review

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World Journal of Gastroenterology 2001年第1期7卷 1-8页

作者： Wen Jie Dai Hong Chi Jiang Second department of General Surgery, the First Clinical School, harbin medical university, harbin 150001, Heilongjiang Province, china Second department of General Surgery the First Clinical School Harbin Medical University Harbin 150001 Heilongjiang Province China. wenjiedai@***

INTRODUCTIONLiver fibrosis or cirrhosis is a common progressively pathological lesion of chronic liver diseases in response to various liver-damaging factors. The main mechanisms of fibrotic or cirrhotic initiation an... 详细信息

关键词： Gene Therapy Humans Liver Cirrhosis Telomere

Current treatment for liver metastases from colorectal cancer

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World Journal of Gastroenterology 2003年第2期9卷 193-200页

作者： Lian-Xin Liu Wei-Hui Zhang Hong-Chi Jiang department of Surgery, the First Clinical College, harbin medical university, harbin 150001, Heilongjiang Province, china department of Surgery First Clinical College Harbin Medical University No. 23 Youzheng Street Nangang District Harbin 150001 Heilongjiang Province China. liulianxin@***

The liver is the commonest site of distant metastasis ofcolorectal cancer and nearly half of the patients withcolorectal cancer ultimately develop liver involved duringthe course of their diseases. Surgery is the only therapythat offers the possibility of cure for patients with hepaticmetastatic diseases. Five-year survival rates after resectionof all detectable liver metastases can be up to 40 %.Unfortunately, only 25 % of patients with colorectal livermetastases are candidates for liver resection, while the othersare not amenable to surgical resection. Regional therapiessuch as radiofrequency ablation and cryotherapy may beoffered to patients with isolated unresectable metastasesbut no extrahepatic diseases. Hepatic artery catheterchemotherapy and chemoembolization and portal veinembolization are often used for the patients with extensiveliver metastases but without extrahepatic diseases, whichare not suitable for regional ablation. For the patients withmetastatic colorectal cancer beyond the liver, systemicchemotherapy is a more appropriate choice. Immunotherapyis also a good option when other therapies are used incombination to enhance the efficacy. Selective internalradiation therapy is a new radiation method which can beused in patients given other routine therapies Without effects.

关键词：结直肠癌肝转移癌肿瘤治疗学化学疗法免疫疗法生存率

Radiofrequence ablation of liver cancers

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World Journal of Gastroenterology 2002年第3期8卷 393-399页

作者： Lian-Xin Liu Hong-Chi Jiang Da-Xun Piao,department of Surgery,the First Clinical College,harbin medical university,harbin 150001,Heilongjiang Province,china department of Surgery the First Clinical College Harbin Medical University No.23 Youzheng Street Nangang District Harbin 150001 Heilongjiang Province China. scott_lxliu@***

Primary and secondary malignant liver cancer are some of most common malignant tumors in the world. Chemotherapy and radiotherapy are not very effective against them. Surgical resection has been considered the only potentially curtive option, but the majority of patients are not candidates for resection because of tumor size, location near major intrahepatic blood vessels and bile ducts, precluding a margin-negative resection, cirrhotic, hepatitis virus infection or multifocial. Radiofrequence ablation (RFA), which is a new evolving effective and minimally invasive technique, can produce coagulative necrosis of malignant tumors. RFA should be used percutaneously, laparascopically, or during the open laparotomy under the guidance of ultrasound, CT scan and MRI. RFA has lots of advantages superior to other local therapies including lower complications, reduced costs and hospital stays, and the possibility of repeated treatment. In general, RFA is a safe, effective treatment for unresectable malignant liver tumors less than 7.0 cm in diameter. We review the principle, mechanism, procedures and experience with RFA for treating malignant liver tumors.

关键词： Carcinoma, Hepatocellular Colorectal Neoplasms Humans Liver Neoplasms Radio Waves Research Support, Non-U.S. Gov't

Specific targeting of gliomas with multifunctional superparamagnetic iron oxide nanoparticle optical and magnetic resonance imaging contrast agents

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Acta Pharmacologica Sinica 2007年第12期28卷 2019-2026页

作者： Xiang-xi MENG~2,Jia-qi WAN~4,Meng JING~2,Shi-guang ZHAO~2,Wei CAI~3,En-zhong LIU~(2,4)~2 department of Neurosurgery,First Affiliated Hospital,harbin medical university,harbin 150001,china,~3 School of Material Science andEngineering,harbin Institute of Technology,harbin 150001,china department of Neurosurgery First Affiliated Hospital Harbin Medical University Harbin China School of Material Science and Engineering Harbin Institute of Technology Harbin China

Aim:To determine whether glioma cells can be specifically and efficiently tar-geted by superparamagnetic iron oxide nanoparticle (SPIO)-fluoresceinisothiocyanate (FITC)-chlorotoxin (SPIOFC) that is detectable by magnetic reso-nance imaging (MRI) and optical ***:SPIOFC was synthesized byconjugating SPIO with FITC and *** cells (human U251-MG andrat C6) were cultured with SPIOFC and SPIOF (SPIO-FITC),*** were treated with SPIOFC as the control for SPIOFC-targeted glioma *** internalization of SPIOFC by glioma cells was assessed by MRI and wasquantified using inductively-coupled plasma emission *** opticalimaging ability of SPIOFC was evaluated by confocal laser scanning ***:Iron per cell of U251 (72.5±1.8 pg) and C6 (74.9±2.2 pg) cells cultured withSPIOFC were significantly more than those of U251 (6.6±1.0 pg) and C6 (7.1±0.8pg) cells incubated with *** T2signal intensity of U251 and C6 cellscultured with SPIOFC (233.6±25.9 and 211.4±17.2,respectively) were substantiallylower than those of U251 and C6 cells incubated with SPIOF (2275.3±268.6 and2342.7±222.4,respectively).Moreover,there were significant differences in ironper cell and T2signal intensity between SPIOFC-treated neural cells (1.3±0.3;2533.6±199.2) and SPIOFC-treated glioma *** internalized by gliomacells exhibited green fluorescence by confocal laser scanning ***:SPIOFC is suitable for the specific and efficient targeting of *** and optical imaging in conjunction with SPIOFC can differentiateglioma cells from normal brain tissue cells.

关键词： superparamagnetic iron oxide nanoparticles gliomas biocompatibility magnetic resonance imaging optical imaging molecular imaging

Detecting robust gene signature through integrated analysis of multiple types of high-throughput data in liver cancer

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Acta Pharmacologica Sinica 2007年第12期28卷 2005-2010页

作者： Xin-yu ZHANG~(2,3,4,6),Tian-tian LI~(5,6),Xiang-jun LIU~(2,3,4,7)~2 department of Biological Science and Biotechnology,~3 School of Biomedicine and ~4 Ministry of Education Key Laboratory of Bioinformatics,Tsinghua university Beijing 100084,china ~5 Laboratory of medical Genetics,department of Biology,harbin medical university,harbin150086.china department of Biological Science and Biotechnology Tsinghua University Beijing China School of Biomedicine Tsinghua University Beijing China Ministry of Education Key Laboratory of Bioinformatics Tsinghua University Beijing China department of Biology Laboratory of Medical Genetics Harbin Medical University Harbin China

Aim:To investigate the robust gene signature in liver cancer,we applied anintegrated approach to perform a joint analysis of a highly diverse collection ofliver cancer genome-wide datasets,including genomic alterations and transcrip-tion ***:1-class Significance Analysis of Microarrays coupled withranking score method were used to identify the robust gene signature in livertumor ***:In total,1 625 051 gene expression measurements from 16public microarrays,2 pairs of serial analyses of gene expression experiments,and252 loss of heterozygosity reports obtained from 568 publications were used inthis integrated *** resulting robust gene signatures included 90 genes,which may be of great importance to liver cancer research.A system assessmentanalysis revealed that our integrative method had an accuracy of 92% and acorrelation coefficient value of ***:The system assessment resultsindicated that our method had the ability of integrating the datasets from varioustypes of sources,and eliciting more accurate results,as can be very useful in thestudy of liver cancer.

关键词： liver cancer integrated analysis robust gene signature gene expression map microarray SAGE

Ex vivo-expanded bone marrow stem cells home to the liver and ameliorate functional recovery in a mouse model of acute hepatic injury

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Hepatobiliary & Pancreatic Diseases International 2012年第1期11卷 66-73页

作者： Shi-Zhu Jin,Bing-Rong Liu,Jun Xu,Fu-Lai Gao,Zong-Jing Hu,Xin-Hong Wang,Feng-Hua Pei,Yu Hong,Hong-Yan Hu and Ming-Zi Han department of Gastroenterology and Hepatology,and department of Science Research Management,Second Affiliated Hospital,harbin medical university,harbin 150080,china department of Gastroenterology and Hepatology,Fourth Affiliated Hospital,harbin medical university,harbin 150001,china department of Gastroenterology and Hepatology Second Affliated Hospital Harbin Medical University Harbin 150080 Harbin China department of Gastroenterology and Hepatology Fourth Affliated Hospital Harbin Medical University Harbin 150001 Harbin China department of Science Research Management Second Affliated Hospital Harbin Medical University Harbin 150080 Harbin China

BACKGROUND:Stem cell transplantation provides a theoretical approach for liver regeneration medicine;it may promote liver regeneration and ***,the transplantation of bone marrow-mesenchymal stem cells expanded ex vivo as a therapy for liver disease has rarely been *** study aimed to explore whether bone marrow stem cells expanded ex vivo home to the liver and foster hepatic recovery after CCl 4 ***:Bone marrow cells from BALB/c mice were expanded ex vivo by multiple-passage cultivation,characterized by cytoflow immunofluorescence,and pre-labeled with PKH26 before intravenous infusion into animals treated with CCl *** integration of bone marrow cells into the liver was examined microscopically,and plasma hepatic enzymes were determined ***:Cultured bone marrow cells exhibited antigenic profiles comparable to those of primary medullary stem *** immunofluorescence showed colocalization of these cells with proliferative activity and albumin expression in the liver of CCl 4 -treated *** showed increased in situ cell proliferation (50±14 vs 20±3 cells/high-power field,P<0.05) and albumin expression (149±25 vs 20±5 cells/high-power field,P<0.05) in the liver,as well as reduced serum aminotransferase levels (P<0.05) and better survival rates (P<0.05) in animals receiving cultured bone marrow cells relative to ***:Ex vivo-expanded bone marrow cells are capable of relocating to and proliferating in the chemically- injured *** of these pluripotent stem cells appears to improve serum indices of liver function and survival rate in mice after CCl4-induced hepatic damage.

关键词： stem cell therapy stem cell culture acute hepatic injury

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Effect of IL-18 on peripheral blood mononuclear cells of chronic hepatitis B and hepatitis B virus DNA released by HepG2.2.15 cell lines

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Hepatobiliary & Pancreatic Diseases International 2004年第2期3卷 230-234页

作者： Ying Sun, Huan-Yong Chen and Shao-Jie Xin harbin, china department of Infectious Diseases, First Clinical Col- lege, harbin medical university, harbin 150001 , china department of Infectious Diseases First Clinical College Harbin Medical University Harbin 150001 China.

BACKGROUND: Interleukin-18 (IL-18), a pro-inflamma- tory cytokine that induces interferon-γ (IFN-γ) production in T cells and natural killer cells, plays a critical role in the T-lymphocyte helper type 1 ( Th1) response. This study was designed to explore the effect of IL-18 on peripheral blood mononuclear cells ( PBMCs) derived from chronic hepatitis B (CHB) and on hepatitis B virus (HBV) DNA released by HepG2.2.15 cell lines, which were transfected with hepatitis B virus gene in vitro. METHODS: PBMCs isolated from 25 healthy people and 25 patients with CHB were stimulated with HBcAg and IL-18 of various concentrations for 72 hours. The levels of IFN-γ in the supernatants of cultured PBMCs were determined by ELISA. After the stimulation of IL-18 of various concentra- tions, PBMCs derived from one patient were co-cultured for 96 hours with HepG2. 2. 15 cells which had been cul- tured for 24 hours, and then the supernatants were collected by centrifugation and used for HBV DNA quantitative as- say. RESULTS: When PBMCs were stimulated by HBcAg and IL-18 at various concentrations, the levels of IFN-γ in the supernatants of CHB groups were much higher than those in normal control groups, at 0.2 ng/ml: t =11.70, P0.05). The content of HBV DNA in the superna- tant of co-cultivation of HepG2. 2. 15 cells and PBMCs without stimulated materials was higher than that stimula-ted by HBcAg and EL-18 at various concentrations of HBc- Ag and IL-18 together with IL-12/IFN-α1lb. CONCLUSION: DL-18 can induce IFN-γ secretion and pro- bably play a key role in the modulation of both innate and ada

关键词： interleukin-18 chronic hepatitis B peripheral blood mononuclear cells HepG2.2.15 cells interferon-γ HBV DNA

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