检索结果-南通市图书馆

Down-regulation of p210bcr/ablby curcumin involves disrupting molecular chaperone functions of Hsp90

Acta Pharmacologica Sinica 2006年第6期27卷 694-699页

作者： Li-xian WU Jian-hua XU~2 Xiu-wang HUANG Kun-zhong ZHANG Cai-xia WEN Yuan-zhong CHEN~3 2 institute of Clinical Pharmacology,Department of Pharmacology,fujian Medical University 3 fujian institute of hematology,fuzhou 350004,china Department of Pharmacology Institute of Clinical Pharmacology Fujian Medical University Fuzhou China fujian institute of hematology Fuzhou China

Aim:To investigate the effects of curcumin（Cur）on p210ber/abllevel in K562 cells,and the relationship between these effects and the molecular chaperone functionsof heat shock protein 90（Hsp90）.Methods:Flow cytometry and Western blotwere used to examine the abundance of p210bcr/abl,Hsp90,p23,Hsp70,and p60Hopin K562 cells treated with *** transcription polymerase chain reaction（RT-PCR）was used to determine the bcr-abl mRNA level in K562 cells treated *** co-immunoprecipitation of p210bcr/abland its molecular chaperones,theimmunoprecipitate was then subjected to Western blot analysis with anti-Hsp90,anti-Hsp70,anti-p23,and ***:An exposure of K562 cells toCur produced time-dependent down-regulation of p210bcr/abl,the inhibition rate ofp210bcr/ablin K562 cells determined by flow cytometry after treatment with Cur 27.2μmol/L for 1h,6h,12h and 24 h was 31.2%,63.7%,81.3% and 94.5%,*** contrast,Cur had almost no influence on bcr-abl mRNA *** withCur for 24 h reduced the association of p210bcr/ablwith Hsp90/p23 complex,whileincreasing the association of p210bcr/ablwith Hsp70/p60Hopcomplex;however,thetotal protein abundance of Hsp90,p23,and p60Hopin K562 cells had no apparentchange,while Hsp70 increased ***:Down-regulation ofp210bcr/ablby Cur involves dissociating the binding of p210bcr/ablwith Hspg0/*** contrast,the association of p210bcr/ablwith HspT0/p60Hopcomplexincreased.

关键词： curcumin molecular chaperones bcr-abl fusion proteins heat shock protein 90

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

ZnPcS2P2-based Photodynamic Therapy Induces Mitochondria-dependent Apoptosis in K562 Cells

引用

Acta Biochimica et Biophysica Sinica 2005年第7期37卷 488-494页

作者： Hui-Fang HUANG Yuan-Zhong CHEN Yong WU fujian institute of hematology,Union Hospital,fujian Medical University,fuzhou 350001,china fujian institute of hematology Union Hospital Fujian Medical UniversityFuzhou 350001 China

Mitochondria play a key role in the regulation of apoptosis induced by numerous antitumorchemotherapeutic and other toxic *** therapy （PDT） exerts significant cellular killingefficacy through either an apoptotic or necrotic cell death *** study investigated the mechanismunderlying the killing effects of a novel amphipathic photosensitizer [di-sulfonated di-phthalimidomethylphthalocyanine zinc （ZnPcS2P2）]-mediated photodynamic therapy （ZnPcS2P2-PDT） on K562 *** evident in the post-PDT cells through the TdT-mediated dUTP nick end labeling （TUNEL） method andDNA fragmentation *** ZnPcS2P2-PDT,K562 cells underwent mitochondria-dependent apoptosisas evidenced by the release of cytochrome c from mitochondria into cytosol,accompanied by mitochondrialmembrane potential （ΔΨm） reduction,indicating the opening of the mitochondrial permeability transitionpore （PTP）.The activities of protease from the caspase family and caspase-3 were also significantly ***,ZnPcS2P2-PDT down-regulated the expression of chimaeric Bcr-Abl oncoprotein,which isthe molecular hallmark of chronic myelogenous leukemia （CML）.

关键词： photodynamic therapy zinc phthalocyanine apoptosis mitochondria K562 cell

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Curcumin synergistically augments bcr/abl phosphorothioate antisense oligonucleotides to inhibit growth of chronic myelogenous leukemia cells

引用

Acta Pharmacologica Sinica 2007年第1期28卷 105-110页

作者： Kun-zhong ZHANG~2 Jian-hua XU~(2,4) Xiu-wang HUANG~2 Li-xian WU~2 Yu SU~2 Yuan-zhong CHEN~32 institute of Clinical Pharmacology,School of Pharmacy,fujian Medical University,fujian 350004,china 3 fujian institute of hematology fujian 350004,china institute of Clinical Pharmacology School of Pharmacy Fujian Medical University Fujian China fujian institute ofhematology Fujian China

Aim:To investigate the growth inhibition effect of the combination of bcr/ablphosphorothioate antisense oligonucleotides（PS-ASODN）and curcumin（cur）,and the possible mechanisms of cur on the chronic myelogenous leukemia cell ***:The K562 cell line was used as a P210bcr/abl-positive cell model invitro and was exposed to different concentrations of PS-ASODN（0-20 μmol/L）,cur（0-20 μmol/L）,or a combination of *** inhibition and apoptosis ofK562 cells were assessed by MTT assay and AO/EB fluorescent staining,*** expression levels of P210bcr/abl,NF-κ3 and heat shock protein 90（Hsp90）were assessed by Western ***:Exposure to cur（5-20 μmol/L）and PS-ASODN（5-20 μmol/L）resulted in a synergistic inhibitory effect on cell *** inhibition was associated with the inhibition of the proliferation and in-duction of *** blot analysis showed that the drugs synergisti-cally downregulated the level ofP210bcr/abland NF-κ*** downregulated Hsp90,whereas no synergism was observed when cur was combined with ***:PS-ASODN and cur exhibited a synergistic inhibitory effect on thecell growth of *** synergistic growth inhibition was mediated throughdifferent mechanisms that involved the inhibition of P210bcr/abl.

关键词： combination drug therapy curcumin abl genes antisense oligonucleotides chronic myelogenous leukemia bcr-abl fusion proteins

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Recombinant fibronectin polypeptide antagonizes hepatic failure induced by endotoxin in mice

引用

Acta Pharmacologica Sinica 2004年第6期25卷 81-86页

作者： Yong WU, Yuan-zhong CHEN2, Hui-fang HUANG, Ping CHEN fujian institute of hematology, Union Hospital, fujian Medical University, fuzhou 350001,china fujian institute of hematology Union Hospital Fujian Medical University Fuzhou 350001

AIM: To study the preventive effect of recombinant human fibronectin (rhFN) polypeptide on hepatic failure in- duced by endotoxin in mice. METHODS: A cDNA fragment coding for Ile1363-Tyr1725 of human FN was inserted into the PinPoint Xa-3 plasmid, and the constructed plasmid was transformed into E coli BL21 (DE3) cells, and then the expression of rhFN polypeptide in DE3 cells was identified through SDS-PAGE. The target protein from the supernatant of bacteria lysate was purified through biotin-affinity chromatography. The bioactivity of the purified rhFN polypeptide was determined with cell adhesiveactivity. The survival rate was observed in endotoxemia mice injected with rhFN polypeptide. The tissue damage in hepatocyte was detected using histology, ultrastructure, and DNA fragmentation assay. RESULTS: The expression of rhFN polypeptide reached approximately 20 % of the total cellular protein. The adhesion ability of rhFN polypeptide was concentration-dependent. The value of EC50 was 0.8 nmol/L. The survival rate of endotoxemia mice sensitized by D-galactosamine (D-GalN) was 60 % in rhFN polypeptide treated group, while that of endotoxemia mice sensitized by D-GalN was 20 % in the control group (P<0.01). Histopathology showed that less necrosis occurred on the hepatocyte of endotoxemia mice in- jected with rhFN polypeptide compared with saline control. Ultrastructure and DNA fragmentation assay showed that no apoptotic hepatocyte was observed in the liver of rhFN-treated endotoxemia mice. CONCLUSION: Re- combinant fibronectin polypeptide antagonizes hepatic failure induced by endotoxin in mice.

关键词： fibronectins recombinant fusion proteins lipopolysaccharides acetylgalactosamine

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Mitochondria-dependent apoptosis induced by a novel amphipathic photochemotherapeutic agent ZnPcS2P2 in HL60 cells

引用

Acta Pharmacologica Sinica 2005年第9期26卷 1138-1144页

作者： Hui-fang HUANG Yuan-zhong CHEN~2 Yong WU fujian institute of hematology,Union Hospital,fujian Medical University,fuzhou 350001,china fujian institute of hematology Union Hospital Fujian Medical University Fuzhou China

Aim:To investigate the mechanism underlying the killing effects of a novelamphipathic photosensitizer,disulfonated diphthalimidomethyl phthalocyaninezinc(ZnPcS2P2),mediated photodynamic therapy(ZnPc-PDT)in human myelog-enous leukemia HL60 ***:After incubation for 5 h with 0.5 μmol/LZnPcS2P2,the HL60 cells were exposed to a light source of 670 nm ***,the cells were detected at different time intervals after *** char-acteristics of apoptosis were detected by observation of ultrastructure assay,DNA fragmentation assay and terminal deoxynucleotidyl transferase deoxyuridinenick-end labeling method(TUNEL).Mitochondria-dependent apoptosis was de-termined by the detection of mitochondrial membrane potential(Δψm),activitiesof caspase family protease and of caspase-3,cytosol cytochrome *** Bcl-2and Bax were detected by immunoblot ***:Evident characteristicsof apoptosis were observed post-ZnPc-PDT with ultrastructure assay,DNA frag-mentation assay and TUNEL *** assay showed that apoptotic ratesin the cells collected from 6 h,12 h and 24 h after PDT were 9.6%,24.4%,and 33.0%,***60 cells underwent mitochondria-dependent apoptosis as a re-sult of cytochrome c release from mitochondria into cytosol accompanied by areduction of Δψ*** activities of caspase family protease and of caspase-3were ***,ZnPc-PDT could remarkably down-regulate the Bcl-2pro-apoptotic protein and up-regulate the anti-apoptotic Bax ***:ZnPc-PDT could induce mitochondria-dependent apoptosis in HL60 cells.

关键词： photodynamic therapy zinc phthalocyanine apoptosis cytochrome c caspases HL60 cells

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Experimental studies of the effects of ZnPcS2P2-based-photodynamic therapy on bone marrow purging

引用

中华医学杂志(英文版) 2005年第2期 19-24页

作者： HUANG Hui fang, CHEN Yuan zhong and WU Yong fujian institute of hematology, Union Hospital, fujian Medical University, fuzhou 350001, china

Background An effective purging technique plays an important role in autologous hematopoietic stem cells transplantation Photodynamic therapy (PDT) provides a novel approach for this purpose This study dealt with the purging effects of disulfodiphthalimidomethyl phthalolcyanine zinc (ZnPcS2P2)based photodynamic therapy (ZnPcPDT) Methods Fluorescence intensity of cell extracts was measured using a fluoresence spectrophotometry The proliferative potency of K562 cells and HL60 cells was detected using MTT colorimetric assay, Typan blue dye exclusion method, colony formation test The proliferative potency of normal hematopoietic cells was evaluated using mixture colonyforming unit (CFUMix), granulocytemacrophage colonyforming unit (CFUGM), and erythrocyte colonyforming unit (CFUE) assays K562 cells were mixed with normal mononuclear cells (MNCs) at ratios of 1∶100 and 1∶1000 for creating the model of simulated remission bone marrow Colony formation test and nestedRTPCR were carried out to detect the residual K562 cells in cell mixture Results After a 5hour incubation with ZnPcS2P2, the content of ZnPcS2P2 in normal MNCs was the lowest value At the same time, the content in K562 cells and HL60 cells was very high Therefore, the time point was selected as the optimal one for irradiating the cell suspensions ZnPcPDT could significantly kill proliferative K562 cells and HL60 cells in a dosedependent manner At the concentration of 10 μg/ml, the inhibitory rate of ZnPcPDT on the colony formation was 100% for K562 cells, 897% for HL60 cells 025 μg/ml ZnPcPDT could completely photoinactivate residual K562 cells in the simulated remission bone marrow Under an identical condition, the inhibitory rates of CFUMix, CFUGM, CFUE were 180%, 186%, and 178% respectively Conclusion ZnPcPDT appears to be a promising approach for bone marrow purging

关键词： photodynamic therapy · zinc phthalocyanine · bone marrow purging · leukemia

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Synthesis and characterization of a strong-fluorescent Eu-containing hydrotalcite-like compound

引用

Science china Chemistry 2010年第6期53卷 1273-1280页

作者： CHEN Hong & ZHANG WenGong institute of Polymer Science,College of Chemistry and Materials Science,fujian Normal University,fuzhou 350007,china institute of Polymer Science College of Chemistry and Materials Science Fujian Normal University Fuzhou China

A novel strong-fluorescent Eu-containing hydrotalcite-like compound (Eu-HTlc) was synthesized using the coprecipitation method,in which aluminum(III) was partially substituted by Europium (III) in the hydrotalcite-like layers,and thenoyltrifluoroacetone,1,10-phenanthroline were dispersed into the anions in the interlayer *** sample was characterized by XRD,XPS,FT-IR,ICP,TG-DSC,TEM and fluorescence spectra,and its composition and structure were *** results indicated that the sample exhibited a characteristic red light (614 nm).The fluorescent lifetime and fluorescence quantum yield of Eu-HTlc were measured to be respectively 893 -s and 66.44%,higher than those of Eu(III)-thenoyltrifluoroacetone1,10-phenanthroline complex [Eu(TTA)3phen].The result of TG-DSC measurement showed the enhanced thermal stability of Eu-HTlc compared with that of MgAl-LDHs and Eu(TTA)*** excellent photoluminescent property and thermal stability,low contents of rare earth ions and ligands,the Eu-HTlc may become one of the novel fluorescent materials with potential applications.

关键词： hydrotalcite-like compound Europium fluorescence intercalation supramolecule

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

GRAIN SIZE AND ACTIVATION ENERGY OF SERRATED YIELDING

引用

Acta Metallurgica Sinica(English Letters) 1990年第1期3卷 10-15页

作者： XIAO Lingang QIAN Kuangwu fuzhou University,fuzhou,fujian,china Correspondent:QIAN Kuangwu Professor,institute for Materials Research,fuzhou University fuzhou,fujian 350002,china

The apparent activation energy of serrated yielding was measured by two different methods for a commercial brass *** results showed that the apparent activation energy of serrat- ed yielding measured by a method involving(m+β)values increases with the grain *** contrast,those measured by another method without involving(m+β)values are all the same for specimens with different grain *** with the observation of the microstructures,the above phenomena have been *** method to measure the true activation energy of serrated yielding has also been proposed.

关键词： serrated yielding activation energy grain size deformation twinning

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Curcumin protects mitochondria from oxidative damage and attenuates apoptosis in cortical neurons

引用

Acta Pharmacologica Sinica 2004年第12期25卷 40-46页

作者： Yuan-gui ZHU2, Xiao-chun CHEN3, Zhi-zhe CHEN4, Yu-qi ZENG, Guang-bin SHI, Yan-hua SU, Xu PENG fujian instituteof Geriatrics, fujian institute of hematology, Union Hospital, fujian Medical University, fuzhou 350001, china 2Now in Rush University Medical Center, Chicago, IL 60612,USA. fujian institute of Geriatrics Union Hospital Fujian Medical University Fuzhou 350001 fujian institute of hematology Union Hospital Fujian Medical University Fuzhou 350001

AIM: To investigate the effect of curcumin on tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in rat cortical neurons and to explore the possible mechanism. METHODS: Primary cultured rat cortical neurons were performed in vitro and cell viability was measured by MTT assay. DNA fragmentation was used to evaluate cell apoptosis. Intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (?Ψm) was deter- mined by flow cytometric assay. Cellular glutathione (GSH) content was measured by spectrophotometer. Bcl-2 family proteins, cytochrome c, cleaved caspase-3, and poly (ADP-ribose) polymerase (PARP) were detected by Western blot. RESULTS: Exposure of tBHP 100 μmol/L to neurons for 60 min resulted in ?Ψm loss and cyto- chrome c release from mitochondria and subsequent activationof caspase-3 and PARP cleavation, and cell apoptosis. After removal of tBHP and then further treatment with curcumin (2.5-20 μmol/L) for 18 h, curcumin abrogated ?Ψm loss and cytochrome c release, blocked activation of caspase3, and altered the expression of Bcl-2 family. Further curcumin treatment also prevented cellular GSH and decreased intracellular ROS generation markedly. Curcumin eventually attenuated tBHP-induced apoptosis in cortical neurons. CONCLUSION: Curcumin may attenuate oxidative damages in cortical neurons by reducing intracellular production of ROS and protecting mito- chondria from oxidative damage.

关键词： Curcumin tert-butyl hydroperoxide apoptosis oxidative stress Bcl-2 family proteins mitochondria Alzheimerdisease

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Complete genome sequence and proteomic analysis of a thermophilic bacteriophage BV1

引用

Acta Oceanologica Sinica 2010年第3期29卷 84-89页

作者： LIU Bin WU Suijie XIE Lianhui institute of Plant Virology Fujian Agriculture and Forestry University Fuzhou 350002 China College of Food Science Fujian Agriculture and Forestry University Fuzhou 350002 China Key Laboratory of Marine Biogenetic Resources Third Institute of Oceanography State Oceanic Administration Xiamen 361005 China

Viruses of thermophiles are of great interest due to their roles in gene transfer, global geochemical cycle and evolution of life on earth. However, the thermophilic bacteriophages have not been studied extensively. In this investigation, a typical bacteriophage BV1 was obtained from a thermophilic bacterium Geobacillus sp. 6k512, which was isolated from an inshore hot spring in Xiamen of china. The BV1 contained a double-stranded linear DNA of 35 055 bp, which encodes 54 open reading frames （ORFs）. Interestingly, eight of the 54 BV1 ORFs shared sequence similarities to genes from human disease-relevant bacteria. Seven proteins of the purified BV1 virions were identified by proteomic analysis. Determination of BV1 functional genomics would facilitate the better understanding of the mechanism for virus-thermophile interaction.

关键词： thermophile bacteriophage genome sequence proteomic analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：