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Acta Pharmacologica Sinica 2006年第6期27卷 741-746页

作者： Yi-xiang MAO~(2,3)Yong-Jing CHEN~2 Yan GE~2 Hong-bing MA~2 Jian-feng YU~2 Hong-ya WU~2 Yu-min HU~2 Qin WANG~2 Qin SHI~2 Xue-guang ZHANG~(2,4)2 Institute of Biotechnology,soochow university,suzhou 215007,china 3 Department of Oncology,first affiliated hospital to soochow university,suzhou 215007,china Institute of Biotechnology Soochow University

Aim:To explore the biofunctions of human B7-H4 generated from ***:The gene of human B7-H4 extracellular region（IgV-like andIgC-like domains）was obtained by PCR from human cDNA FLJ22418 and theninserted into the prokaryotic expression vector pGEX-5X-3 expressing glutathiones-transferase（GST）fusion *** being identified by restriction enzymedigestion and sequencing,the recombinant vector was transferred into host strainE coli BL21-RIL（DE3）.A 47 kDa fusion protein（GST/hB7-H4）was induced byisopropyl-beta-D-thiogalactopyranoside（IPTG）and purified by standard meth-ods reported in the prokaryotic *** inhibitory effect of GST/hBT-H4 onproliferation of T cells was observed in vitro by CD3mAb activated T-cell cultur-ing system and [3H]-thymidine incorporation *** concentrations ofinterleukin-2 and iterferon-g in the supernatants of T cells were determined ***:We successfully constructed the method for high-level expres-sion and purification of the hB7-H4 extracellular domain as GST fusion proteinfrom E *** GST/hB7-H4 fusion protein produced in bacteria had obviousbiological activity to inhibit T-lymphocyte proliferation and IL-2 ***:The prokaryote expression system could be used to generate hB7-H4 protein with natural spatial conformations and biological functions,whichprovided an efficient and economical way for the preparation of this target protein.

关键词： B7-H4 glutathione s-transferase fusion protein inhibition

Preliminary clinical application of dopamine transporter imaging with technetium-99m TRODAT-1 and SPECT on the early and differential diagnosis of Parkinson's Disease

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Nuclear Science and Techniques 2002年第4期13卷 211-217页

作者： WANG Feng, WANG Zi-Zheng, LIU Zeng-Li, TANG Jun, LUO Wei-Feng,WU Jin-Chang(1Nuclear Medicine Department, Nanjing No.1 hospital affiliated to Najijing Medicaluniversity, Nanjing 21.006 2Nuclear Medicine Department, Second hospital affiliated to suzhou university, NuclearMedicine Research Institute of suzhou university, suzhou 215004 3Neurology Department, Second hospital affiliated to suzhou university, suzhou 215004) Nuclear Medicine Department Nanjing No. 1 Hospital Affiliated to Nanjing Medical University Nanjing 210006 Nuclear Medicine Department Second Hospital Affiliated to Suzhou University Nuclear Medicine Research Institute of Suzhou University 江苏 Suzhou 215004 Neurology Department Second Hospital Affiliated to Suzhou University 江苏 Suzhou 215004

The aim of the study was to demonstrate the degeneration of the dopaninergic nigrostriatal pathway in Parkinson's disease(PD) and Essential Tremor (ET) by using the cocaine derivative 99mTc-TRODAT-1 SPECT and correlate the findings to the clinical severities (Hoehn and Yahr scale, H/Y). 28 patients with idiopathic Parkinson's disease, 10 patients with Essential Trenor and 19 healthy volunteers were investigated. The acquisition were performed 3 h postinjection of 99mTc-TRODAT-1, ROIs were drawn over the images of striatum and cerebellum, and ratios of striatum to cerebellar(ST/CB) were calculated. Ratios differed significantly between PD and controls, but ratios didn't show significant difference between ET patients and controls. A significant correlation didn't exist between ratios and clinical severities. Hemiparkinson's patients revealed significantly diminished 99mTc-TRODAT-1 binding not only clinically affected but unaffected side. Our findings indicated that 99mTc-TRODAT-1 SPECT is not only a reliable method to discriminate between PD and controls but also a useful tool for differential diagnosis in clinically unclear cases such as ET resembling PD.

关键词：多巴胺输运造影 ^99mTc Parkinson病诊断

Cloning,Structural Organization and Chromosomal Mapping of Rat Costimulatory Molecule 4-1BBL

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Acta Biochimica et Biophysica Sinica 2005年第10期37卷 694-701页

作者： Qiu-Ming DONG~1 Li-Jie MA~2 Guang-Bo ZHANG~1 Ya-Fang WU~3 Jia-Yao SHEN~1 Ying CHEN~1 Yong-Jing CHEN~1 Xiang-Ke PU~1 Sai-Yu HANG~1 Xue-Guang ZHANG~(1*) 1 Biotechnology Institute,soochow university,suzhou 215007,china 2 Genetics Institute,Fudan university,Shanghai 200433,china 3 first affiliated hospital of soochow university,suzhou 21.006,china BiotechnologyInstitute SoochowUniversitySuzhou215007China

4-1BBL (TNFSF9) is a member of the tumor necrosis factor (TNF) ligand superfamily,whichis expressed on some activated antigen presenting cells and B *** isolated a rat cDNA clone encodingthe rat homologue of the human 4-1BBL (GenBank accession ***259541).The deduced rat 4-IBBLprotein,consisting of 308 amino acids with a molecular weight of 33,469 Da,was a typical type Ⅱtransmembrane glycoprotein,the same as human and routine 4-IBBL.“SDAA” in the cytoplasmic domain ofrat 4-1BBL was deduced to act as the phosphorylation site for casein kinase Ⅰ(“SXXS” motif),which ispresent in the cytoplasmic domains of human and murine 4-1BBL,and all other TNF ligand family membersknown to utilize reverse *** two introns of 4-1BBL were also cloned (GenBank accession ***332409).Rat 4-IBBL is much more homologous with murine 4-1BBL than with human 4-1BBL,in bothnucleotide and amino acid *** 4-1BBL was expressed in all tested tissues:brain,lung,colon,liver,thymus,testicle,kidney,adrenal,stomach,spleen and *** chromosomal location of rat 4-1BBL wasfirst identified by bioinformatics,then by fluorescence in situ hybridization at 9q11(GenBank accessionUni Gene ***.46783).Rat,murine and human 4-1BBL genes are evolved from a common *** and characterization of the rat counterpart of human 4-1BBL will facilitate studies of the biologicalfunction of this molecule.

关键词： TNF superfamily rat 4-1BBL cloning mapping

Association between Serum Homocysteine and Oxidative Stress in Elderly Patients with Obstructive Sleep Apnea/hypopnea Syndrome

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Biomedical and Environmental Sciences 2010年第1期23卷 42-47页

作者： LING WANG,JIE LI 2,YAN XIE,AND XUE-GUANG ZHANG Laboratory of Clinical Immunology,the first affiliated hospital of soochow university,suzhou 21.006,Jiangsu,china [a] Laboratory of Clinical Immunology the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu China

Objective Elderly patients with obstructive sleep apnea/hypopnea syndrome （OSAHS） has a higher risk of cardiovascular and cerebrovascular disease. However, changes of homocysteine （Hey） as markers of cardiovascular and eerebrovascular disease associated with OSAHS and their mechanism have not been elucidated so far. This study aims to investigate the changes of both serum Hcy and oxidative stress and their possible links with OSAHS in elderly patients. Methods Based on polysomnogram （PSG） and age, 83 patients with OSAHS were recruited and divided into elderly-OSAHS （n=32） and non-elderly OSAHS groups （n=51）. Fifty two subjects without OSAHS were divided into elderly control （n=29） and non-elderly control groups （n-23）. A total of 135 subjects were included in the present study. All subjects were recorded for PSG variables and the contents of homocysteine （Hcy）, malonaldehyde （MDA）, and glutathione （GSH） which were detected after sleep. Serum homocysteine was measured by cyclophorase. MDA and GSH were measured by speetrophotometer. Results （1） The serum levels of Hcy showed significant difference among the four groups （P〈0.05）. The concentrations of Hcy in elderly OSAHS patients were higher than in other groups, while those in the elderly control group were higher than in the non-elderly control; the concentrations in the non-elderly OSAHS group were higher than in the non-elderly control. （2） The concentrations of MDA and GSH changed at an equal pace with Hcy in the four groups. （3） Multielement linearity regression analysis indicated a statistically significant relationship between Hcy concentration and age, MDA, GSH, and apnea hypopnea index （AHI）. Conclusions （1） The concentrations of Hcy and oxidative stress have increased with advancing age. （2） The concentrations of Hcy and oxidative stress have further increased in the elderly patients with OSAHS. （3） Oxidative stress might cause high-level serum Hcy in the elderly patients with OSAHS.

关键词： Elderly Obstructive sleep apnea/hypopnea syndrome Homocysteine Oxidative stress Malonaldehyde Glutathione

Research progresses in the pathogenesis of anaplastic largecell lymphoma

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Chinese Journal of Cancer 2011年第6期30卷 392-399页

作者： Xiao-Lan Shi,Xiao-Wen Tang and De-Pei Wu Department of Hematology,the first affiliated hospitalof soochow university,Jiangsu Institute of Hematology Key Laboratoryof Thrombosis and Hemostasis of Ministry of Health,suzhou,Jiangsu21.006,P.R.china Department of Hematology the First Affiliated Hospital of Soochow University Jiangsu 215006 People's Republic of China.

Anaplastic large cell lymphoma(ALCL) is a distinct subset of T-cell non-Hodgkin's *** a consequence of its low incidence,general pathogenic consideration of ALCL is *** this review,we summarize the pathogenesis,epidemiology,clinical manifestations,and treatment of ALCL,so as to better understand key stages of the development of this disease and provide valuable information for future treatment.

关键词：淋巴瘤发病机制细胞变性发病机理流行病学临床表现发病率

Spiral CT localization of pancreatic function-ing islet cell tumors

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Hepatobiliary & Pancreatic Diseases International 2004年第4期3卷 616-619页

作者： Qing Xu, Wen-Hua Chen and Qing-Juan Huang Nanjing, china Department of Radiology, first affiliated hospital of Nanjing Medical university, Nanjing 210029, china Department of Radiology, Third affiliated hospital of suzhou Univer- sity, Changzhou 213003, china Department of Radiology First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China. xuqingst@***

BACKGROUND: Surgeons are always concerned about the localization of pancreatic functioning islet cell tumor. If the tumor is accurately localized before operation, resection of the pancreatic body and tail without intention can be avoided. The purpose of this study was to evaluate spiral CT localization of pancreatic functioning islet cell tumors and CT techniques. METHODS: CT manifestations in 6 patients with clinically and pathologically proved pancreatic functioning islet cell tumors ware analyzed retrospectively. RESULTS: In 4 patients with insulinomas and 2 patients with glucagonomas, 5 were localized accurately by CT be- fore surgery and 1 was detected retrospectively. The en- hancement of tumors was greater than that of normal pan- creas in arterial phase and pancreatic parenchymal phase. Four patients showed mild high-density and 2, iso-density in the portal venous phase. CONCLUSION: Spiral CT multi-phase enhanced scan with 1.5 ml/kg contrast agent and 2-5 mm slice width can loca- lize functioning islet cell tumors accurately.

关键词： islet cell tumor spiral CT enhanced scan

GABAA receptor partially mediated propofol-induced hyperalgesia at superspinal level and analgesia at spinal cord level in rats

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Acta Pharmacologica Sinica 2004年第12期25卷 53-59页

作者： Qin-yun WANG2,3, Jun-li CAO2, Yin-ming ZENG2, Ti-jun DAI2,4 2Jiangsu Institute of Anesthesiology, Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, 221002, china 3Department of Anesthesiology, the1st affiliated hospitalof soochow university, suzhou 21.006, china Jiangsu Institute of Anesthesiology Jiangsu Province Key Laboratory of Anesthesiology Department of Anesthesiology the 1st Affiliated Hospital of Soochow University

AIM: To observe effects of propofol on nociceptive response at superspinal and spinal level in rats. METHODS: Two hundreds and fifty-eight Sprague-Dawley male rats were randomized into thirty-two groups. Propofol and bicuculline were microinjected into lateral ventricle (icv), ventrolateral periaqueductal gray (vlPAG), intrathecal (ith), and intraperitoneal (ip). The noxious responses were evaluated by hot plate and formalin test. RESULTS: In hot-plate test, systemic and superspinal administration of propofol (40 mg·kg-1 ip, 100 μg in 10 μL, icv, and 4 μg in 0.4 μL vlPAG microinjection) produced hyperalgesia (P30 min by microinjection of bicuculline (10 ng in 0.4 μL, vlPAG) (P3 %, 54.8 %, 80.8 %, and 97.4 % at 10, 20, 30 and 40 min by ith bicuculline (P30 min by ith bicuculline (P<0.05) after formalin injection. Hyperalgesia produced by ip propofol in both hot plate and formalin test could not be antagonized by vlPAG administration of bicuculline. CONCLUSION: GABAA receptor partly mediated propofol-induced hyperalgesia at superspinal and analgesia at spinal cord in rats.

关键词： GABA receptors propofol pain ventrolateral periaqueductal gray spinal cord

Triptolide inhibits NF-kB activation and reduces injury of donor lung induced by ischemia/reperfusion

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Acta Pharmacologica Sinica 2007年第12期 1919-1923页

作者： Jing-kang HE~1,Shu-dong YU~1,Hong-Jun ZHU~1,Jun-chao WU~2,Zheng-hong QIN~(2,3)~1 Department of Cardiothoracic Surgery,the first hospital affiliated to soochow university,suzhou 21.006,china ~2 Department of Pharma-cology and Laboratory of Aging and Nervous Diseases,School of Medicine,soochow university,suzhou 215123,china

Aim:To investigate the protective effect of triptolide （TRI） on ischemia/reperfusion-induced injury of transplanted rabbit lungs and to investigate the mechanismsunderlying the actions of ***:We established the rabbit lung trans-plantation model and studied lung injury induced by ischemia/reperfusion andthe inhibitory effect of TRI on *** severity of lung injury was determinedby a gradual decline in PvO2,the degree of lung edema,the increase in themyeloperoxidase （MPO） activity,and the ultrastructural changes of *** activation of NF-kB was measured by *** in intercellular adhesion molecule-1 （ICAM-1）,which is the target gene ofNF-kB,was evaluated by ***:After reperfusion,there was a gradualdecline in the PvO2level in the control group （group Ⅰ）.The level of PvO2in thegroup treated with lipopolysaccharide (group Ⅱ was significantly decreased,whereas that of the group treated with TRI （group Ⅲ） was markedly improved（P<0.01）.In group Ⅲ,the activity of MPO was downregulated,and the pulmonaryedema did not become severe and the ultrastructure of the donor lung *** activity of NF-kB and the expression of ICAM-1 was significantlyincreased in the donor *** blocked NF-kB activation and ***:The effects of TRI on reducing injury to donor lungsinduced by ischemia/reperfusion may possibly be mediated by inhibiting theactivity of NF-kB and the expression of the NF-kB target gene ***,TRI could be used in lung transplantations for improving the function of donorlungs.

关键词： lung transplantation ischemia/reperfusion injury triptolide NF-kB inter-cellular adhesion molecule-1

Cellular basis of abnormal calcium cycling of failing rabbit myocytes and effects of metoprolol and losartan

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Cellular basis of abnormal calcium cycling of failing rabbit...

Increasing the frequency of CIK cells adoptive immunotherapy may decrease risk of death in gastric cancer patients

11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-suzhou 2006

作者： Cao Zou & Zhi-hua Liu Department of Cardiology, The first affiliated hospital soochow university,suzhou, china

Objective: In this study,we established animal model of heart failure induced by volume plus pressure overload in rabbits in order to investigate cellular basis of abnormal calcium cycling of failing *** we observed t... 详细信息

关键词： Heart failure Calcium cycling Calcium regulatory protein L-type calcium channel(LTCC) Calcium release channel(Ryr2) SERCA2a Na+-Ca2+ exchanger(NCX) Metoprolol Losartan

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World Journal of Gastroenterology 2010年第48期16卷 6155-6162页

作者： Jing-Ting Jiang, Chang-Ping Wu, Lu-Jun Chen, Xiao Zheng, Department of Tumor Biological Treatment, Third affiliated hospital of soochow university, Changzhou 213003, Jiangsu Province, china Yi-Bei Zhu, Jing Sun, Xue-Guang Zhang, Key Laboratory of Stem Cell of Jiangsu Province, Institute of Biotechnology, Key Laboratory of Clinical Immunology of Jiangsu Province, soochow university, suzhou 215123, Jiangsu Province, china Yue-Ping Shen, Wen-Xiang Wei, Department of Medicine, soochow university, suzhou 215123, Jiangsu Province, china Bin-Feng Lu, Department of Immunology, university of Pitts- burgh School of Medicine, Pittsburgh, PA 15261, United States Key Laboratory of Stem Cell of Jiangsu Province Institute of Biotechnology Key Laboratory of Clinical Immunology of Jiangsu Province Soochow University Suzhou 215123 Jiangsu Province China. xueguangzh@***

AIM: To analyze the correlation between cytokineinduced killer (CIK) cells adoptive immunotherapy and cancer-related death in gastric cancer patients. METHODS: One hundred and fifty-six gastric cancer patients after operation at the Third affiliated hospital of soochow university were enrolled in this study. Their clinical data including demographic characteristics, operation time, tumor size, pathological type and staging, tumor metastasis, outcome of chemotherapy or CIK cells adoptive immunotherapy, survival time or time of death were collected with a standard structured questionnaire. Kaplan-Meier method was used to estimate the median survival time, and the 2- and 5- year survival rates. Hazard risk (HR) and 95% confidence interval (95% CI) of CIK cells adoptive immunotherapy for gastric cancer were calculated using the two-stage time-dependent covariates Cox model. RESULTS: The survival time of gastric cancer patients was longer after CIK cells adoptive immunotherapy than after chemotherapy (χ 2 = 10.907, P = 0.001). The median survival time of gastric cancer patients was also longer after CIK cells adoptive immunotherapy than after chemotherapy (49 mo vs 27 mo, P first stage Cox model was used to define the subjects who remained alive beyond 36 mo as survivors. However, no correlation was observed between the frequencies of deat

关键词： Immunotherapy Cytokine-induced killer cells Gastric cancer Survival analysis Probability