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Expression of lignin peroxidase H2 from Phanerochaete chrysosporium by multi-copy recombinant Pichia strain

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Journal of Environmental Sciences 2009年第2期21卷 218-222页

作者： WANG Wei,WEN Xianghua department of Environmental Science and engineering,tsinghua university,beijing 100084,china. department of Environmental Science and engineering Tsinghua University Beijing 100084 China

The lipH2 gene, encoding the expression of lignin peroxidase, was cloned from Phanerochaete chrysosporium BKM-F-1767 and expressed in Pichia pastoris X-33, a yeast. The cDNA of LiPH2 was generated from total RNA extracted from P chrysosporium by PCR with primers that do not contain a P. chrysosporium lignin peroxidase secretion signal. The gene was then successfully inserted into the expression vector pPICZα, and resulted in the recombinant vector pPICZα-lipH2. The transformation was conducted in two ways. One was using the wild Pichia pastoris as the recipients, which results in the recombinant P. pastoris with single or low lipH2 gene copy. The second was using P. pastoris and single or low lipH2 gene copy as the recipients, which results in the recombinant P. pastoris with multi-copies of lipH2 genes. This study firstly expressed the gene lipH2 in P. pastoris and achieved the successful expression of the lipH2 depending upon the generation of a recombinant strain that contained multiple copies. The lignin peroxidase activity reached a maximum of 15 U/L after 12 h induction.

关键词： heterologous expression lignin peroxidase Pichia pastoris

An atlas-based fuzzy connectedness method for automatic tissue classification in brain MRI

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Progress in Natural Science:Materials International 2006年第10期16卷 1106-1110页

作者： ZHOU Yongxin and BAI Jing (department of Biomedical engineering, tsinghua university, beijing 100084, china) department of Biomedical engineering Tsinghua University Beijing 100084 China

A framework incorporating a subject-registered atlas into the fuzzy connectedness (FC) method is proposed for the automatic tissue classification of 3D images of brain MRI. The pre-labeled atlas is first registered onto the subject to provide an initial approximate segmentation. The initial segmentation is used to estimate the intensity histograms of gray matter and white matter. Based on the estimated intensity histograms, multiple seed voxels are assigned to each tissue automatically. The normalized intensity histograms are utilized in the FC method as the intensity probability density function (PDF) directly. Relative fuzzy connectedness technique is adopted in the final classification of gray matter and white matter. Experimental results based on the 20 data sets from IBSR are included, as well as comparisons of the performance of our method with that of other published methods. This method is fully automatic and operator-independent. Therefore, it is expected to find wide applications, such as 3D visualization, radiation therapy planning, and medical database construction.

关键词： fuzzy connectedness atlas-based segmentation brain tissue classification MR1.

Study on Numerical Simulation of Mold Filling and Heat Transfer in Die Casting Process

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Journal of Materials Science & Technology 2000年第3期16卷 269-272页

作者： Liangrong JIA, Shoumei XIONG and Baicheng LIU (department of Mechanical engineering, tsinghua university, beijing 100084, china) department of Mechanical engineering Tsinghua University Beijing 100084 China

A 3-D mathematical model considering turbulence phenomena has been established based on a computational fluid dynamics technique, so called 3-D SOLA-VOF (Solution Algorithm-Volume of Fluid), to simulate the fluid flow of mold filling process of die casting. In addition, the mathematical model for simulating the heat transfer in die casting process has also been established. The computation program has been developed by the authors with the finite difference method (FDM) recently. As verification, the mold filling process of a S-shaped die casting has been simulated and the simulation results coincide with that of the benchmark test. Finally, as a practical application, the gating design of a motorcycle component was modified by the mold filling simulation and the dies design of another motorcycle component was optimized by the heat transfer simulation. All the optimized designs were verified by the production practice.

关键词： Study on Numerical Simulation of Mold Filling and Heat Transfer in Die Casting Process Mold Simulation

Semi-solid Processing of Al-7Graghite Composite

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Journal of university of Science and Technology beijing 2001年第3期8卷 203-206页

作者： Peng Zhang, Yunhui Du, Daben Zeng department of Mechanical engineering, tsinghua university, beijing 100084, china department of Mechanical engineering Tsinghua University Beijing 100084 China

Al-7graphite composite was processed using Al-7graphite mushy prepared by electromagnetic-mechanical stirring method, and the influence of solid fraction on the distributing of graphite particles in ingot was studied. The results shows that the relationship between solid fraction and stirring temperature of mushy is: f(s) = 591.5-0.897 t (where f(s) is the solid fraction, t is the stirring temperature). For Al-7graphite composite, with the increasing of solid fraction, the aggregation extent of graphite particles reduced gradually, and when solid fraction was larger than 30%, graphite particles could distribute evenly in ingot.

关键词： Al-7graphite composite electromagnetic-mechanical stirring method distributing of graphite particles solid fraction stirring temperature

Stability　Analysis　of　Nonlinear　Vibrations　of　a　Deploying　Flexible　Beam

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Communications in Nonlinear Science and Numerical Simulation 1996年第4期1卷 32-35页

作者： Junfeng LI & Zhaolin WANG (department of engineering mechanics, tsinghua university, beijing 100084,china) E-mail: lijf@*** department of engineering mechanics Tsinghua University Beijing 100084China

ＳｔａｂｉｌｉｔｙＡｎａｌｙｓｉｓｏｆＮｏｎｌｉｎｅａｒＶｉｂｒａｔｉｏｎｓｏｆａＤｅｐｌｏｙｉｎｇＦｌｅｘｉｂｌｅＢｅａｍ１，２１ＰｒｏｊｅｃｔｓｕｐｐｏｒｔｅｄｂｙｔｈｅＮａｔｉｏｎａｌＮａｔｕｒａｌＳｃｉｅｎｃ... 详细信息

ＳｔａｂｉｌｉｔｙＡｎａｌｙｓｉｓｏｆＮｏｎｌｉｎｅａｒＶｉｂｒａｔｉｏｎｓｏｆａＤｅｐｌｏｙｉｎｇＦｌｅｘｉｂｌｅＢｅａｍ１，２１ＰｒｏｊｅｃｔｓｕｐｐｏｒｔｅｄｂｙｔｈｅＮａｔｉｏｎａｌＮａｔｕｒａｌＳｃｉｅｎｃｅＦｏｕｎｄａｔｉｏｎｏｆＣｈｉｎ...

关键词： Deploying flexible beam Rigid-flexible coupled system Partial stability

Thermodynamic Significance of Human Basal Metabolism

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Journal of Thermal Science 1993年第2期2卷 81-86页

作者： Wang Cuncheng Thermal engineering department tsinghua university, beijing 100084, P.R. china Thermal engineering department tsinghua university Beijing P.R. China

The human basal state,a non-equilibrium steady state,is analysed in this paper in the light of the First and Second Laws of Thermodynamics whereby the thermodynamic significance of the basal metabolic rate and its distinction to the dissipation function and exergy loss are *** analysis demonstrates the correct expression of the effects of the blood flow on the heat balance in a human-body bio-heat model and the relationship between the basal metabolic rate and the blood perfusion.

关键词： thermodynamic laws basal metabolism blood perfusion dissipation function exergy loss

A SINGLE-PERIOD INVENTORY SYSTEM WITH A GENERAL S-SHAPED UTILITY AND EXPONENTIAL DEMAND

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Journal of Systems Science and Systems engineering 2010年第2期19卷 227-236页

作者： Wei GENG1 Xiaobo ZHAO2 Deng GAO3 department of Industrial engineering,tsinghua university,beijing 100084,china department of Industrial engineering Tsinghua University Beijing China

We discuss a single-period inventory system with a general S-shaped utility function and exponential demand. It is shown that there exists a state-dependent order-up-to policy to be optimal. Behaviors of optimal polic... 详细信息

关键词： Inventory S-shaped utility function order-up-to policy

Studies of the unsteady supersonic base flows around three afterbodies

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Acta Mechanica Sinica 2009年第4期25卷 471-479页

作者： Zhixiang Xiao Song Fu School of Aerospace engineering,tsinghua university, 100084 beijing, china School of Aerospace engineering Tsinghua University Beijing China

Unsteady supersonic base flows around three afterbodies, cylindrical （Cy）, boattailed （BT） and three-step （MS）, are investigated in this paper. Reynolds-averaged Navier-Stokes （RANS） and two RANS/LES （large-eddy simulation） hybrid methods, detached eddy simulation （DES） and delayed-DES （DDES）, are used to predict the base flow characteristics around the baseline Cy afterbody. All the RANS and hybrid methods are based on the two-equation SST （shear-stress transport） model with compressible corrections （CC）. According to the comparison of measurements, both DES and DDES can produce more satisfactory results than RANS. RANS can only present the ＂stable＂ flow pat- terns, while the hybrid methods can demonstrate unsteady flow structures. DDES and DES results are little different from one another although the latter exhibits better agreement with the experiment. DES is taken to investigate the 5° BT and three-step afterbodies. The mean flow data and the instantaneous turbulent coherent structures are compared against available measurements.

关键词： Cylindrical Boattailed and three-stepafterbodies DES and DDES Unsteady flow

THE EXISTENCE OF J-HOLOMORPHIC CURVES AND APPLICATIONS TO THE WEINSTEIN CONJECTURE

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Chinese Annals of Mathematics,Series B 1999年第4期20卷 425-434页

作者： MA RENYI(department of Applied Mathmatics, tsinghua university, beijing 100084, china) department of Applied Mathmatics Tsinghua University Beijing 100084 China

The author first proves the existences of J-holomorphic curves in the symplectizations of Legendre fibrations and then as an application confirms the Weinstein conjectures on contact manifolds of Legendre fibrations. As a corollary a new proof on the theorem due to Hofer,Viterbo, Gluck, Ziller, Weinstein and Ljusternik-Fet Theorem is provided, which is quite different from their original proofs.

关键词： Symplectic geometry J-holomorphic curves Periodic orbit