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Inhibitory effect of rosiglitazone on C-reactive protein production induced by angiotensin Ⅱ in human aortic endothelial cells via ROS and mitogen activated protein kinases signaling

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Inhibitory effect of rosiglitazone on C-reactive protein pro...

中国药理学会第十一次全国学术会议

作者： HAN Chun-jie~1,LIU Jun-tian~2,CUI Mu~(2,3),LI Ming~2,PENG Xiao-ming~2,MAO Jun-jun~2 (1.department of Pharmacology,Shannxi Institute For Food And Drug Control,Xi’an 710061,China 2.department of Pharmacology,Xi’an Jiaotong university School of Medicine,Xi’an 710061,’ China 3.Nursing department,Xi’an Medical university,Xi’an 710021,China)

OBJECTIVE To observe the effects of rosiglitazone on signaling pathway of angiotensinⅡ(AngⅡ) -induced C-reactive protein(CRP) expression in human aortic endothelial cells(HAECs).METHODS HAECs were pretreated with the different concentrations of rosiglitazone 18 h before stimulation with Ang II for 12 ***,mRNA of CRP and AT,was analyzed by RT-PCR, and the protein expressions of CRP,ATI,p-JNK,p-ERK1/2,JNK and ERK1/2 in HAECs were analyzed by western blot respectively. HAECs were pretreated with losartan,PD123319 for 1 h or rosiglitazone for 18 h prior to stimulation with AngⅡfor 12 ***, the cells were incubated for 1 h with ***,the fluorescent intensity was measured by a fluorescence ***- SULTS Pretreatment of the cells with rosiglitazone for 18 h obviously reduced AngⅡ-induced mRNA and protein expressions of CRP and *** and rosiglitazone obviously inhibited AngⅡ-stimulated ROS generation,while PD123319 showed no significantly inhibitory effect on ROS production in *** of the cells with rosiglitazone for 18 h obviously downregulated AngⅡ-caused phosphorylation of ERK1/2 and JNK in ***,rosiglitazone is able to inhibit AngⅡ-induced CRP expression in HAECs,and probably through interfering with AT-ROS-MAPK signal *** As an inflammatory molecule,CRP plays a direct role in *** previous study confirmed that AngⅡis capable of inducing CRP generation in HAECs,which is through AT-ROS-ERK1/2 and JNK-NF-κB signaling *** the present study,we observed that rosiglitazone attenuated AngⅡ-induced CRP expression in HAECs,which implies that rosiglitazone may counteract vascular inflammation and thus exerts a beneficial effect in prevention of *** is well known that most of the deleterious effects of AngⅡare accomplished through AT *** present results showed that AngⅡupregulates AT expression,whereas rosiglitazone reduced AngⅡ-induced AT expression in *** AT recept

关键词： rosiglitazone angiotensinⅡ C-reactive protein endothelial cells

吲哚美辛对乙酸诱导大鼠胃溃疡形成和愈合的影响

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复旦学报（医学版） 2001年第3期28卷 243-247页

作者：沈锡中复旦大学附属中山医院消化科上海200032

目的　利用乙酸诱导大鼠胃溃疡模型研究吲哚美辛对化学诱导胃溃疡形成和愈合的影响 ,探讨其可能机制。方法　雄性SD大鼠 ,体重 16 0～ 180 g。分两组 ,即单纯乙酸诱导胃溃疡作为对照组和乙酸诱导胃溃疡加吲哚美辛处理组 ,各时间点每组... 详细信息

目的　利用乙酸诱导大鼠胃溃疡模型研究吲哚美辛对化学诱导胃溃疡形成和愈合的影响 ,探讨其可能机制。方法　雄性SD大鼠 ,体重 16 0～ 180 g。分两组 ,即单纯乙酸诱导胃溃疡作为对照组和乙酸诱导胃溃疡加吲哚美辛处理组 ,各时间点每组均 8只。乙酸诱导胃溃疡后 1、3和 7d用RT PCR和Westernblotting分别检测胃黏膜中环氧合酶 (COX)和诱导型一氧化氮合酶 (iNOS)的表达。用ELISA测定胃黏膜中PGE2 量反映COX活性。同时研究吲哚美辛对iNOS表达、活性及胃黏膜损伤的影响 ,以溃疡面积来评估胃黏膜损伤程度。结果　RT PCR结果显示乙酸诱导大鼠胃溃疡后 ,COX2 mRNA表达明显升高 ;以溃疡基底部为明显 ,3d最高 ,7d下降。胃黏膜PGE2 合成也明显增高。吲哚美辛能抑制胃黏膜PGE2 合成 ,处理组溃疡面积 1d时为 (5 2 .6± 6 .1)mm2 ,小于对照组的 (71.8± 5 .8)mm2 (P 3d时两组溃疡大小无差异 ,但吲哚美辛处理组溃疡基底部厚度为 (11± 0 .5 )mm ,薄于对照组的 (2 0± 0 .8)mm(P 35 .4± 3.5 )mm2 ,大于对照组的 (2 4.8± 3.2 )mm2 (P <0 .0 5 )。此外吲哚美辛能降低胃黏膜iNOS的表达及活性。结论　吲哚美辛能减轻大鼠溃疡形成初期炎症反应。

关键词：环氧合酶胃溃疡一氧化氮合酶诱导型吲哚美辛乙酸诱导

Exploring the common pathogenesis pathways in autoimmune diseases by integrate data and literatures from genetics,genomics and epigenetic modification of autoimmunity

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Exploring the common pathogenesis pathways in autoimmune dis...

The 8th International Congress on SLE

作者： Hai-Tao Yang department of Medicine Division of rheumatology & Immunology Keck School of Medicine university of Southern California 2011 Zonal Ave.HMR Room#704 Los Angeles California 90033 USA

＜正＞Identification of common determinants that underlie pathogenesis of autoimmune diseases will open a window onto the pathways that lead to develop new strategies for diseases *** progress of autoimmune diseases r...

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Study of the relationship between renal injury of systemic lupus erythematosus and autoantibodies

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Study of the relationship between renal injury of systemic l...

The 8th International Congress on SLE

作者： Chen Weifei~1 Zuo Xiaoxia~2 Luo Hui~2 Zhao Hongjun~2 1 department of Nephrology,Xinhua Hospital,Hainan Medical College,Haikou,570311,China 2 department of rheumatology & Immunology,Xiangya Hospital,Central South university,Changsha,410008,China

＜正＞Purpose:To investigate the serum antinuclear antibody （ANA）,double stranded DNA（dsDNA）,anti-nucleosome antibody（AnuA）and anti-extractable nuclear antigen（ENA）

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Thromboxane receptor stimulation triggers pro-inflammatory responses in endothelial cells by activating ROCK/LKB1/IKK-mediated IκB phosphorylation

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中国病理生理杂志 2012年第11期 2093-2094页

作者： HE Jin-long1,2,ZOU Ming-hui2,ZHU Yi1(1department of Physiology and Pathophysiology,Peking university Health Sciences Center,Beijing 100191,China 2Section of Molecular Medicine,department of Medicine,university of Oklahoma Health Sciences Center,Oklahoma City,OK 73104,USA)

AIM:Aberrant activation of thromboxane A2(TXA2)receptor(TPr)is reported to cause vascular inflammation and *** TPr activation instigates vascular inflammation remains poorly *** aim of the present study was to determi... 详细信息

关键词： IKK ROCK B phosphorylation

Sildenafil attenuates LPS-induced pro-inflammatory responses through down-regulation ROS-related MAPK/NF-kappaB as well as cGMP related signaling pathways in N9 microglia

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Sildenafil attenuates LPS-induced pro-inflammatory responses...

第五届中日基础和临床药理学双边学术会议

作者： Jingyu Yang~1,Siqi Zhao~1,Lijia Zhang~1,Guoning Lian~1,Xiaoxiao Wang~1,Haotian Zhang~1,Xuechun Yao~1,Jiming Wang~2,Chunfu Wu~(1*) 1 department of Pharmacology,Shenyang Pharmaceutical university,Shenyang,China 2 Laboratory of Molecular Immunoregulation,Cancer and inflammation Program, Center for Cancer research,national Cancer Institute at Frederick,Frederick,MD,USA

＜正＞Excessive activation of microglial cells has been implicated in various *** present study showed that sildenafil,a PDE5 inhibitor,significantly suppressed NO production through decreasing the protein and/or mRNA expressions of inducible NO synthase（iNOS）,as well as decreased the mRNA expressions and release of interleukin 1β（IL-1β） and tumor necrosis factorα（TNF-α）.induced by LPS in microglial

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Oligomeric Procyanidins Induce Generation of Reactive Oxygen Species and Collapse of Mitochchondrial Membrane Potential in Glioblastoma Cell Lines

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Chinese Herbal Medicines 2009年第1期1卷 45-52页

作者： ZHANG Feng-jiao1, YANG Jing-yu1, MOU Yan-hua1, SUN Bao-shan2, WANG Ji-ming3, WANG Fang1, WU Chun-fu1 1. department of Pharmacology, Shenyang Pharmaceutical university, Shenyang 110016, China 2. Estaco Vitivinícola Nacional, Instituto National de Investigaco Agrária, Dois Portos 2565-191, Portugal 3. Laboratory of Molecular Immunoregulation, Cancer and inflammation Program, Center for Cancer research, National Cancer Institute at Frederick, Frederick Maryland 21702, USA department of Pharmacology Shenyang Pharmaceutical UniversityShenyang 110016China Estacao Vitivinícola Nacional Instituto National de Investigacao AgráriaDois Portos 2555-191Portugal Laboratory of Molecular Immunoregulation Cancer and Inflammation ProgramCenter for Cancer ResearchNational Cancer Institute at FrederickFrederick Maryland 21702USA

Objective The aim of the present study was to clarify the mechanism underlying glioma cell death upon oligomeric procyanidins (F2) exposure. Methods The cytotoxicity of F2 on U87 (human malignant glioblastoma cell line) and C6 (rat glioma cell line) cancer cells was evaluated, and changes of mitochondrial membrane potential (MMP) and production of reactive oxygen species (ROS) in drug-treated cells were monitored. Moreover, morphological changes associated with F2-induced cells death were examined. Results F2 induced a concentration-dependent increase in ROS production and decrease in MMP. Furthermore, pre-incubation with N-acetylcysteine (NAC) and rotenone (Rt), resulted in partial inhibition of F2-induced ROS generation and marked attenuation of cell death and the cytoplasmic vacuolization induced by F2. In addition, pretreatment with Rt markedly attenuated the MMP loss in F2-treated cells. However, pretreatment with NAC only markedly attenuated the MMP loss in F2-treated C6 cells. Conclusion The increase in ROS level is at least one of mechanisms associated with F2-induced glioma cell death as well as the cytoplasmic vacuolization formation that contribute to the cytotoxicity of F2 in glioma cells.

关键词： glioma mitochondrial membrane potential oligomeric procyanidins paraptosis reactive oxygen species

System A transporters in spinal cord is involved in central sensitization induced by formalin in rat

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System A transporters in spinal cord is involved in central ...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Jing WANG~1,Bin GEN~1,Tian-Cheng WANG~2,Ya-Yi XIA~1,Hai-Li SHEN~3,Zhi-Ping WANG~(4,*) 1 Institute of Orthopaedics,Lanzhou university Second Hospital,Lanzhou 730030,China 2 department of Neurology,Lanzhou university Second Hospital,Lanzhou 730030,China 3 department of Pain,Lanzhou university Second Hospital,Lanzhou 730030,China 4 department of urology,Lanzhou university Second Hospital,Lanzhou 730030,China

＜正＞Objective There is evidence that glia-neuron interactions are involved in mechanisms underlying the development of chronic *** sensitization is a state of increased excitability of nociceptive neurons in the spinal dorsal horn following peripheral tissue injury and/or inflammation and is considered a crucial process underlying the development and maintain of chronic pain *** is growing evidence that astroglia play an important role in chronic *** plays a major role in central sensitization and astrocyte-neuronal glutamate-glutamine cycle is involved in central sensitization in the spinal *** A transport is responsible for the accumulation of glutamine by neurons and non-metabolised amino acid analogue a-（methylamino）isobutyric acid（MeAIB）appears to be a specific inhibitor of all isoforms of this *** current study investigated the roles of System A transport in inhibition of central sensitization induced by formalin in *** A total of 43 male,Sprague Dawley rats,aged 4 months,were randomly assigned to a sham operation group（n=6）and a model group（n= 37）.Rats in the model group received intrathecal infusion operation at spinal cord.7 days later,37 model rats were randomly divided into saline,MeAIB 0.05 mM,MeAIB 0.1 mM,MeAIB 0.3 mM,MeAIB 0.5 mM,and MeAIB 1.0 mM and formalin subcutaneous injection alone *** 15 min after intrathecal injection,a rat model of formalin-induced inflammatory pain was established by subcutaneous injection of 5%formalin（50μL）in the left hindpaw. After the completion of behavioral measurements,rats were sacrifice and the spinal cords were removed for GFAP immunohistochemistry *** In this study,intrathecal injection MeAIB produced a dose-dependent inhibition of inflammatory pain,including nociceptive behaviors and GFAP expression in the spinal dorsal *** control experiments,intraplantar injection of formalin alone or intrathecal injection of saline did not produce a

关键词： system A transport glutamate-glutamine shuttle central sensitization inflammatory pain spinal cord

Promising diagnostic model of pulmonary tuberculosis constructed by using MALDI-TOF MS technology and weak cationic exchange magnetic beads

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Promising diagnostic model of pulmonary tuberculosis constru...

中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Chuiwen Deng~1,Minggui Lin～3, Chaojun Hu～1,Yanfeng Li～3,Yang Gao～4,Xiaoxing Cheng～3,Fengchun Zhang～1, Mei Dong～2,Yongzhe Li～1 1 department of rheumatology and Clinical Immunology,Peking Union Medical College Hospital,Peking Union Medical College and Chinese Academy of Medical Sciences 2 department of Clinical Laboratory,309th Hospital of Chinese PLA,Beijing 3 ivision of research,Institute of Tuberculosis, 309th Hospital of Chinese PLA,Beijing 4 department of Immunology,Institute of Basic Medical Sciences,Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing

Aims Pulmonary tuberculosis(TB) is an infectious disease that disturbs the balanced status of public *** diagnosis of TB would effectively help controlling the *** proteomic fingerprint profiling by matrix-assisted laser desorption-ionization time of flight mass spectrometry(MALDI-TOF MS) combined with pretreatment of magnetic beads is a robust approach

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