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Experimental Study on the Influence of Yun Zhi Polysaccharopeptide upon Telomerase Activity during Oral Leukoplakia Carcinogenesis

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Experimental Study on the Influence of Yun Zhi Polysaccharop...

2004年上海市口腔医学学术年会

作者： Lou Jianing , Zhou Zengtong department of Stomatology, shanghai first People''s hospital, shanghai Jiaotong university department of Oral medicine , shanghai ninth People''s hospital, shanghai second medical university

＜正＞ Objectives: To study the intervention and dynamic effect of Yun Zhi Polysaccharopeptide（PSP） on telomerase activity during Syrian golden hamsters cheek porch carcinogenesis induced by 7,12-Dimethylbenz[a] anthracene （DMBA）. To explore the possible mechanism in suppressing oral carcinogenesis of PSP. Methods;Salley method was adopted to produce the model of OLK carcinogenesis, while PSP is used in vivo. 115 golden hamsters, ranging from 4 to 8 weeks and 80 to120 grams, were divided into four groups: control group （A）: 10 hamsters were sacrificed from 4 to 8 weeks for control;Model group （B）: 45 hamsters were pasted in bilateral cheek with 0.5% DMBA, 3 times a week for establishing of experimental leukoplakia, and sacrificed from 4 to 8 weeks for model;experimental group: 90 hamsters were divided into two subgroups: PSP pre-administration（C）: 30 hamsters underwent oral taking PSP for 12 weeks before DMBA pasting and PSP oral taking continuously, which were sacrificed at 16 to 20 weeks. PSP post-administration（D）: 30 hamsters underwent simultaneous DMBA pasting and oral taking PSP, which were sacrificed at 4 to 8 weeks. Double blind was applied to detect the dynamic pathologic changes. TRAP-ELISA was used to examine the effect of PSP on telomerase activity. Results: The pathology and the rates of telomerase positive were significant difference between administration group and control group（P＜0.05 or P＜0.01）. The telomerase activity were significant difference at the pathologic stage of epithelial mid-dysplasia among groups（P＜0.05）. Conclusions: It is convincing that PSP have the sure effect of inhibiting OLK carcinogenesis. Telomerase activity may be one of the many targets which PSP act its anti-cancer effect on. the best phrase that PSP play their role in inhibiting telomerase activity is at epithelial mid-dysplasia stage.

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严重急性呼吸道综合征冠状病毒疫苗研究现状

生物化学与生物物理进展 2003年第3期30卷 331-334页

作者：叶迅孟夏董继斌梁旻胡放陈红专上海第二医科大学药理学教研室上海200025 复旦大学药学院生物化学教研室上海200032 上海三维生物技术有限公司

冠状病毒被认为是新近爆发的严重急性呼吸道综合征的病原体 .迄今公共数据库中已发表了不同国家和地区分离的 12个SARS病毒株基因组完整序列 .突起蛋白是冠状病毒的主要抗原 ,包含许多抗原决定簇 .SARS冠状病毒突起蛋白的相对保守性为... 详细信息

冠状病毒被认为是新近爆发的严重急性呼吸道综合征的病原体 .迄今公共数据库中已发表了不同国家和地区分离的 12个SARS病毒株基因组完整序列 .突起蛋白是冠状病毒的主要抗原 ,包含许多抗原决定簇 .SARS冠状病毒突起蛋白的相对保守性为有效疫苗的开发奠定了很好的研究基础 .灭活或减毒的冠状病毒疫苗存在一定的局限性和危险性 .

关键词：严重急性呼吸道综合征冠状病毒疫苗突起蛋白 SARS 基因组

DC dielectrophoresis separation of marine algae and particles in a microfluidic chip

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Science china Chemistry 2012年第4期55卷 524-530页

作者： SONG YongXin1, YANG JianDong1, SHI XiaoFei4, JIANG Hai3, WU YanBin1, PENG Ran1, WANG Qi5, GONG Ning2, PAN XinXiang1, SUN YeQing2 & LI DongQing1,31department of Marine Engineering, Dalian Maritime university, Dalian 116026, china 2Institute of Environmental Systems Biology, Dalian Maritime university, Dalian 116026, china 3department of Mechanical and Mechatronics Engineering, university of Waterloo, Waterloo, ON, N2L 3G1, Canada 4College of Information Science and Technology, Dalian Maritime university, Dalian 116026, china 5department of respiratory medicine, the second hospital Affiliated to Dalian medical university, Dalian 116026, china department of Marine Engineering Dalian Maritime University Dalian China College of Information Science and Technology Dalian Maritime University Dalian China department of Mechanical and Mechatronics Engineering University of Waterloo Waterloo Canada department of respiratory medicine the Second Hospital Affiliated to Dalian Medical University Dalian China Institute of Environmental Systems Biology Dalian Maritime University Dalian China

This paper reports a microfluidic method of continuous separation of marine algae and particles by DC dielectrophoresis. The locally non-uniform electric field is generated by an insulating PDMS triangle hurdle fabricated within a PDMS microchannel. Both the particles and algae are subject to negative DEP forces at the hurdle where the gradient of local electric-field strength is the strongest. The DEP force acting on the particle or the algae depends on particles' or algae's volume, shape and dielectric properties. Thus the moving particles and algae will be repelled to different streamlines when passing the hurdle. In this way, combined with the electroosmotic flow, continuous separation of algae of two different sizes, and continuous separation of polystyrene particles and algae with similar volume but different shape were achieved. This first demonstration of DC DEP separation of polystyrene particles and algae with similar sizes illustrates the great influence of dielectric properties on particle separation and potentials for sample pretreatment.

关键词： DC dielectrophoresis – marine algae – particle – microfluidic chip – permittivity difference

Chronic intermittent hypoxia increases beta cell mass and activates mTOR/HIF1/VEGF-A pathway in mice islet

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Chronic intermittent hypoxia increases beta cell mass and ac...

中华医学会呼吸病学年会——2011（第十二次全国呼吸病学学术会议）

作者： Chenjuan Gu,Min Li,Qingyun Li,Ning Li department of respiratory medicine,shanghai Ruijin hospital,medical school of shanghai Jiaotong university

The objective of this study was to determine changes in pancreatic beta cell mass and islet mTOR/HIF1/VEGF-A pathway in mice subjected to chronic intermittent hypoxia,which was probably related to CIH-induced impaired glucose metabolism and beta cell dysfunction. A gas control delivery system was designed to regulate the flow of nitrogen and oxygen into a customized

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精氨酸对慢性阻塞性肺疾病合并营养不良的免疫调理作用

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中国实用内科杂志 2003年第9期23卷 527-529页

作者：徐卫国罗勇杨玲上海第二医科大学附属新华医院呼吸内科 200092

目的　研究精氨酸对慢性阻塞性肺疾病 (COPD)合并营养不良患者的免疫调理作用。方法　对 30例营养不良的COPD病人进行总能量摄入均为静息能量消耗 (REE) 1 5倍的营养支持。A组 (12例 )为精氨酸治疗组 ,每日口服L 精氨酸 30 g ,B组 (18... 详细信息

目的　研究精氨酸对慢性阻塞性肺疾病 (COPD)合并营养不良患者的免疫调理作用。方法　对 30例营养不良的COPD病人进行总能量摄入均为静息能量消耗 (REE) 1 5倍的营养支持。A组 (12例 )为精氨酸治疗组 ,每日口服L 精氨酸 30 g ,B组 (18例 )为对照组。结果　A组TSF、PAlb、CHI等营养参数明显较B组改善 ,A组TNF α、CD8治疗后显著下降 ,而IL 2、NO、IgG、CD4 等显著上升 (P <0 0 5 )。结论　精氨酸对COPD合并营养不良患者不仅具有辅助营养支持作用 ,而且还有免疫调理作用。

关键词：精氨酸慢性阻塞性肺疾病营养不良免疫调理作用合并症

Increased frequency of the CTLA-4 +49A>G polymorphism in Chinese patients with colorectal cancer compared to healthy controls

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Increased frequency of the CTLA-4 +49A>G polymorphism in Chi...

全国临床免疫检验研讨会暨第六届全国临床免疫学术会议

作者： Peng Qi~1,Can-ping Ruan~2,Tong-hai Dou~3,Hao Wang~4,Fei-guo Zhou~5,Xin-yun Xu~2,Xing Gu~1,Yun-peng Zhao~1,Chun-fang Gao~(*1) 5.department of Laboratory medicine,Eastern Hepatobiliary hospital,second Military medical university,shanghai 200438,china 6.department of General Surgery,Changzheng hospital,second Military medical university,shanghai 200003,china 7.Institute of Genetics,Fudan university,shanghai 20043,china 8.department of Laboratory medicine,Changzheng hospital,second Military medical university,shanghai 200003,china 9.department of Hepatic Surgery,Eastern Hepatobiliary hospital,second Military medical university,shanghai 200438,china.

Objective Colorectal cancer is one of the most common malignancies in the world and a multipathway *** T-lymphocyte antigen-4(CTLA-4) is a potent immunoregulatory molecule that suppresses antitumor response by down-regulating T-cell *** most studied +49A>G polymorphism of CTLA-4 gene has been associated with various kinds of *** aim was to investigate the association between this genetic variant and the risk and progression of colorectal cancer in Chinese. Method We conducted a case-control study of 124 colorectal cancer cases and 407 healthy *** was extracted from blood specimens,and +49A>G polymorphism in the CTLA-4 gene was genotyped by polymerase chain reaction-ligation detection reaction(PCR-LDR).Results In our study group,the frequency of AG or GG or carrying at least one G allele at position +49 was significantly different in colorectal cancer patients and the control group,indicating that the risk of CRC was significantly higher among subjects with the AG or GG genotype or carrying at least one G allele at position +49 than among the subjects with the AA ***,we observed no association between CTLA-4 +49A>G polymorphism and the progression of CRC including tumor stage,tumor grade,status of lymph node and distant ***,the CTLA-4 +49A allele was in non-significantly higher numbers in CRC patients with distant *** Our results suggested that CTLA-4 +49A>G polymorphism was associated with an increased risk of colorectal cancer,but this polymorphism did not play an important role in Chinese patients with CRC.

关键词： Colorectal cancer cytotoxic T-lymphocyte antigen-4 single-nucleotide polymorphism case-control study individual susceptibility

N-glycan Based Models Improve Diagnostic Efficacies in HBV-related Hepatocellular Carcinoma

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N-glycan Based Models Improve Diagnostic Efficacies in HBV-r...

全国临床免疫检验研讨会暨第六届全国临床免疫学术会议

作者： Meng Fang,MD,Yun-Peng Zhao,MD,Lun-Gen Lu,MD,Peng Qi,Fei-Guo Zhou,MD,Hao Wang,Kun Zhou,Shu-Han Sun,PhD,Cui-Ying Chen,PhD,Chun-Fang Gao,MD 1 department of Laboratory medicine,Eastern Hepatobiliary hospital,second Military medical university, shanghai,china 2 department of Gastroenterology,renji hospital,Jiaotong university School of medicine,shanghai,china 3 department of Laboratory medicine,Changzheng hospital,second Military medical university,shanghai, china 4 department of Genetics,second Military medical university,shanghai,china 5 department for Molecular Biomedical Research,Ghent university and Flanders Interuniversity Institute for Biotechnology(VIB),Ghent,Belgium.

BACKGROUND:The early diagnosis of hepatocellular carcinoma(HCC) is of great clinical desirable due to lack of specific and sensitive *** in the sugar chains of glycoprotein synthesized by the liver contribute to the molecular basis of abnormalities in *** the study aims to construct and assess the diagnostic value of N-glycan based diagnostic model in identifying ***:A total of 273 cases HBV related HCC(n=145),liver fibrosis(n=128) and matched healthy controls (n=120) were recruited.N-glycome of serum protein was profiled by DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis(DSA-FACE). Multiparameters diagnostic models were constructed based on above profiling and routine ***:A biantennary glycan(NG1A2F,peak 4) was found to be decreased in HCC and increased in liver fibrosis,while a branching triantennary glycan(NA3Fb,peak 9) was increased in HCC and decreased in liver *** efficacy of the log ratio of these two N-glycan structure abundances[log(p9/4)]was similar to AFP in differentiation HCC from *** accuracy and sensitivity of the diagnostic model combining AFP and N-glycan markers(Cscore B)were increased 7-10%compared to that of AFP. Log(p9/4) was more efficient in monitoring the progression of HCC in regarding to vascular invasion with improved specificity(16%) and accuracy(8%) compared to that of AFP. CONCLUSIONS:The branchingα(1,3)-fucosylated triantennary glycan and the biantennary glycan are potential as N-glycan *** diagnostic models based on the N-glycan markers and AFP improve the efficacy in HCC diagnosis and progression monitoring.

关键词： hepatocellular carcinoma(HCC) liver fibrosis hepatitis B virus(HBV) N-glycan marker

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重叠综合征患者夜间缺氧和睡眠结构的分析

上海医学 2003年第12期26卷 899-901页

作者：王佳林建海鲁慧敏上海第二医科大学附属新华医院呼吸科 200092

目的　探讨重叠综合征 (OS)患者夜间缺氧和睡眠结构的变化。方法　对 19例OS、2 5例阻塞性睡眠呼吸暂停综合征 (OSAS)、17例慢性阻塞性肺疾病 (COPD)和 14例鼾症患者分别进行全夜多导睡眠仪 (PSG)检查 ,并对各组数据作统计学t检验。结... 详细信息

目的　探讨重叠综合征 (OS)患者夜间缺氧和睡眠结构的变化。方法　对 19例OS、2 5例阻塞性睡眠呼吸暂停综合征 (OSAS)、17例慢性阻塞性肺疾病 (COPD)和 14例鼾症患者分别进行全夜多导睡眠仪 (PSG)检查 ,并对各组数据作统计学t检验。结果　单纯鼾症患者基本不存在日间或夜间缺氧 (P 3+ 4 )显著低于COPD患者 (P <0 .0 1) ,动脉血氧饱和度 (SaO2 ) <90 %累计时间的百分率 (SLT90 % )、SaO2 <80 %累计时间占睡眠时间的百分率 (SLT80 % )及浅睡眠所占百分率 (S1+ 2 )显著高于COPD患者 (P <0 .0 1)。OS患者SLT90 %、S1+ 2显著高于OSAS患者 (P <0 .0 1) ,睡眠效率显著低于OSAS患者 (P <0 .0 1)。结论　OS患者夜间缺氧较严重 ,睡眠结构改变明显。

关键词：重叠综合征夜间缺氧睡眠结构阻塞性睡眠呼吸暂停综合征慢性阻塞性肺疾病鼾症多导睡眠仪夜间低氧血症

活血方对免疫介导再生障碍性贫血小鼠IL-3、IFN-γ的影响

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复旦学报（医学版） 2003年第6期30卷 553-555页

作者：丁敬远黄振翘孙关林盛民立周永明黄韬王克强薛志忠上海华东疗养院中医科无锡214065 上海中医药大学附属岳阳医院血液科上海200437 上海第二医科大学附属瑞金医院血液科上海200025 复旦大学放射医学研究所第四研究室上海200032 复旦大学附属中山医院心血管病研究所上海200032

目的检测免疫介导再生障碍性贫血(再障)小鼠骨髓造血细胞在含活血药血清液体培养体系中IL-3、IFN-γ的浓度,探讨活血方药治疗再障的作用机制。方法用活血方中药煎剂灌喂SD大鼠,抽取、制备含中药血清;Balb/c小鼠经60Co 5.5 Gy γ射线... 详细信息

目的检测免疫介导再生障碍性贫血(再障)小鼠骨髓造血细胞在含活血药血清液体培养体系中IL-3、IFN-γ的浓度,探讨活血方药治疗再障的作用机制。方法用活血方中药煎剂灌喂SD大鼠,抽取、制备含中药血清;Balb/c小鼠经60Co 5.5 Gy γ射线照射后,由尾静脉输入取自DBA/2小鼠胸腺淋巴结混合细胞悬液,形成免疫介导再障模型。在含活血药血清的液体培养体系中培养再障小鼠骨髓造血细胞,检测培养体系中IL-3、IFN-γ的浓度。结果正常活血血清组IL-3浓度显著高于正常对照血清组(P3浓度则显著低于再障对照血清组(P0.05)。结论活血方药临床治疗再障的作用可能是影响IL-3的生成而进一步促进造血干/祖细胞增殖、分化。

关键词：活血方免疫介导再生障碍性贫血小鼠 IL-3 IFN-γ 白细胞介素3 γ-干扰素