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Protective effect of steroidal saponins from rhizome of Anemarrhena asphodeloides on ovariectomy-induced bone loss in rats

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Acta Pharmacologica Sinica 2006年第6期27卷 728-734页

作者： Hua NIAN~2 Lu-ping QIN~2 Wan-sheng CHEN~3 Qiao-yan ZHANG~(2,4)Han-chen ZHENG~2 Yin WANG~5 2 department of Pharmacognosy,School of pharmacy,second military medical university,shanghai 200433.china 3 department of pharmacy,changzheng hospital,second military medical university,shanghai 200003,china 5 № 455 hospital of PLA,shanghai 200052,china department of Pharmacognosy School of Pharmacy Second Military Medical University Shanghai China department of pharmacy Changzheng Hospital Second Military Medical University Shanghai China No 455 hospital of PLA Shanghai China

Aim:To investigate the protective effect of steroidal saponins from Anemarrhenaasphodeloides（ATS）on ovariectomy（OVX）-induced bone ***:Sprague-Dawley rats were divided into sham and OVX *** OVX ratswere treated with vehicle,nylestriol or steroidal saponins extract for 12 *** calcium,phosphorus,estradiol（E2）,osteocalcin concentration and serumalkaline phosphatase activity were *** density was assayed by dual-energy X-ray *** undecalcified longitudinal proximal tibial meta-physical（PTM）sections were cut and stained for histomorphometric analysis ofthe ***:In OVX rats,alkaline phosphatase activities in serum weremarkedly increased and concentrations of osteocalcin were decreased by ATStreatment,which had no influence on the body ***,atrophy of theuterus and descent of bone mineral density（BMD）was suppressed by treatmentwith *** addition,ATS completely corrected the decreased the concentrationof calcium and E2in serum observed in OVX *** results showedATS prevented decreases in trabecular thickness and increases in trabecular sepa-ration of proximal tibia metaphysis（PTM）in OVX ***,it did not alterosteoclast number in OVX ***,ATS（300 mg/kg）had a remarkableeffect on promoting bone formation action in OVX *** treatmentdecreased the bone formation rate and mineral apposition ***:Anadequate supply of steroidal saponins of Anemarrhena asphodeloides preventedOVX-induced bone loss in rats through the promotion of bone formation but notthe inhibition of bone resorption.

关键词： Anemarrhena asphodeloides ovariectomy osteoporosis serum bone mineral density histochemistry

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Isoform of Vascular Endothelial Cell Growth Inhibitor (VEGI72-251) Increases Interleukin-2 Production by Activation of T Lymphocytes

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 249-253页

作者： Jing-Juan YAO~1 Min ZHANG~1 Xiao-Hui MIAO~2 Ping ZHAO~1 Shi-Ying ZHU~1 Hui DING~1 Zhong-Tian QI~1 1 Key Laboratory for medical Microbiology,PLA,department of Microbiology,second military medical university,shanghai 200433,china 2 department of Infectious Diseases,changzheng hospital,second military medical university shanghai 200433,china Key Laboratory for medical Microbiology PLA Department of Microbiology Second Military Medical UniversityShanghai 200433 China department of Infectious Diseases Changzheng Hospital Second Military Medical UniversityShanghai 200433 China

The objective of this study is to investigate the characteristics of the recombinant variant ofhuman vascular endothelial cell growth inhibitor,VEGI72-251,and compare its biological activities with that ofits prototype VEGI24-174,The recombinant plasmid containing the variant VEGI72-251gene was constructedand expressed in Escherichia *** effects of the expressed VEGI72-251on cell proliferations were checkedin the human umbilical vein endothelial cell line and certain tumor cell lines （ECV304 and B 16）.The inhibitionof VEGI72-251on angiogenesis was detected in the chorioallantoic membrane of chick *** comparisonwith VEGI24-174,the recombinant human VEGI72-251seems to have no effect on the proliferation of endothelialcells and the angiogenesis of the chorioallantoic membrane in *** enzyme-linked immunosorbent assay-based method was used for the measurement of interleukin-2 （IL-2） production by peripheral blood monocytes（PBMCs） treated with *** were pretreated with VEGI72-251（1.25-12.50μg/ml） for 24 h invitro,and the IL-2 concentration in PBMC medium was increased from 354 pg/ml to 1256 pg/*** can beconcluded that VEGI72-251is able to increase the level of human IL-2 production by the activation of *** from VEGI24-174on anti-angiogenesis,VEGI72-251may serve as an anti-cancer factorthrough its activation of T lymphocytes.

关键词： vascular endothelial cell growth inhibitor biological activity interleukin-2

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CYP2E1 Pst Ⅰ/Rsa Ⅰ polymorphism and colorectal cancer risk:A meta-analysis

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World Journal of Gastroenterology 2010年第23期16卷 2949-2953页

作者： Guo-Wu Zhou,Jia Hu,Qiang Li department of Respiration,Changhai hospital,second military medical university,shanghai 200433,china department of Oncology,Changhai hospital,second military medical university,shanghai 200433,china department of Respiration Changhai Hospital Second Military Medical University Shanghai 200433 China.

AIM:To clarify the association between CYP2E1 PstⅠ/RsaⅠ polymorphism and susceptibility to colorectal ***:A meta-analysis based on 10 eligible casecontrol studies involving 4979 cases and 6012 controls was carried out to summarize the data on the association between CYP2E1 RsaⅠ/PstⅠ polymorphism and colorectal cancer ***:In comparison of the homozygote c2c2 and c2 carriers(c1c2 + c2c2) and the homozygous wild-type genotype(c1c1),no association was found between CYP2E1 RsaⅠ/PstⅠ polymorphism and colorectal cancer risk [odds ratio(OR)=1.24(95% CI:0.93-1.66) for c2c2;OR = 1.02(95% CI:0.88-1.19) for c2 carriers].In stratif ied analysis,Caucasians with c2c2 homozygote appeared to have an increased risk of colorectal cancer(OR=2.67,95% CI:1.03-6.89,P=0.043),no signif icant associations were found in other ***:c2c2 homozygote of CYP2E1 PstⅠ/RsaⅠ polymorphism may be associated with the increased risk of colorectal cancer in Caucasians,which needs further investigations.

关键词： Cytochrome P450 2E1 PstⅠ/RsaⅠ polymorphism Colorectal cancer Cancer susceptibility Metaanalysis

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Phage display selection on whole cells yields a small peptide specific for HCV receptor human CD81

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Cell Research 2003年第6期13卷 473-479页

作者： JIE CAO, PING ZHAO, XIAO HUI MIAO2, LAN JUAN ZHAO, LI JUN XUE, ZHONG TIAN QIdepartment of Microbiology, second military medical university, shanghai 200433, chinaE-mail: qizt@*** department of Infectious Diseases, changzheng hospital, second military medical university, shanghai200003, china department of Microbiology Second Military Medical University Shanghai China department of Infectious Diseases Changzheng Hospital Second Military Medical University Shanghai China

The human CD81 (hCD81), the most recently proposed receptor of hepatitis C virus (HCV), can especifically bind to HCV envelope glycoprotein 2 (E2). In this study, hCD81-expressing murine NIH/3T3 cells were used to select hCD81-binding peptides from a phage displayed nonapeptide library (PVIII9aaCys). Eighteen of the 75 clones selected from the library showed specific binding to the hCD/81-expressing NIH/3T3 cells by enzyme linked immunosorbent assay (ELISA) and competitive inhibition test. Twelve out of the 18 clones shared the amino acid motif SPQYWTGPA. Sequence comparison of the motif showed no amino acid homology with the native HCV E2. The motif-containing phages could competitively inhibit the ability of HCV E2 binding to native hCD81-expressing MOLT-4 cells, and induce HCV E2 specific immune response in vivo. These results suggest that the selected motif SPQYWTGPA should be a mimotope of HCV E2 to bind to hCD81 molecules. Our findings cast new light on developing HCV receptor antagonists.

关键词： viral receptor hepatitis C virus cell-based selection hCD81-binding peptide phage display.

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Inhibition of Tumor Growth in Mice by Endostatin Derived from Abdominal Transplanted Encapsulated Cells

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Acta Biochimica et Biophysica Sinica 2007年第4期39卷 278-284页

作者： Huaining TENG~(1.3) Ying ZHANG~2 Wei WANG~2 Xiaojun MA~2* Jian FEI~1* 1 Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,Model Organism Research Center,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 200031,china 2 Laboratory of Biomedical Material Engineering,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dolian 116023,china 3 department of Urology,changzheng hospital,second military medical university,shanghai 200003,china Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell Biology Model Organism Research Center Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China department of Urology Changzheng Hospital Second Military Medical UniversityShanghai 200003 China Laboratory of Biomedical Material Engineering Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian 116023 China

Endostatin,a C-terminal fragment of collagen 18a,inhibits the growth of established tumorsand metastases in vivo by inhibiting ***,the purification procedures required for large-scale production and the attendant cost of these processes,together with the low effectiveness in clinicaltests,suggest that alternative delivery methods might be required for efficient therapeutic use of *** the present study,we transfected Chinese hamster ovary(CHO)cells with a human endostatin geneexpression vector and encapsulated the CHO cells in alginate-poly-L-lysine *** release ofbiologically active endostatin was confirmed using the chicken chorioallantoic membrane *** encap-sulated endostatin-expressing CHO cells can inhibit the growth of primary tumors in a subcutaneous B 16tumor model when injected into the abdominal cavity of *** results widen the clinical applicationof the microencapsulated cell endostatin delivery system in cancer treatment.

关键词： microencapsulation endostatin CHO tumor gene therapy

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Adenovirus-expressed preS2 antibody inhibits hepatitis B virus infection and hepatic carcinogenesis

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World Journal of Gastroenterology 2012年第4期18卷 349-355页

作者： Qian Zhang,department of Infectious Diseases,Changhai hospital,second military medical university,shanghai 200433,china Zhi-Qing Li,Hu Liu,Jia-He Yang,department of Hepatic Surgery,Eastern Hepatobiliary Surgery hospital,second military medical university,shanghai 200438,china Qian Zhang Department of Infectious Diseases Changhai Hospital Second Military Medical University Shanghai 200433 China.

AIM:To investigate the inhibitory effect of hepatitis B virus (HBV) preS2 antibody (preS2Ab) against HBV in-fection and HBV-associated hepatic ***:An adenoviral vector carrying the full-length light and heavy chains of the HBV preS2Ab gene,Ad315-preS2Ab,was *** linked immunosorbent assay (ELISA) and Western blotting analyses were used to determine the preS2Ab expres-sion levels in *** techniques were used to examine the binding affinity between the expressed HBV preS2Ab and HBV-positive liver *** were also used to determine hepatitis B surface antigen (HBsAg) levels to assess the inhibitory effect of the preS2Ab against HBV infection in L02 *** inhibitory effect of preS2Ab against hepatic carcinogen-esis was studied with diethylnitrosamine (DEN)-induced hepatocellular carcinomas (HCCs) in HBV transgenic ***:The expression of HBV preS2Ab increased with increases in the multiplicity of infection (MOI) of Ad315-preS2Ab in L02 cells,with 350.87 ± 17.37 μg/L of preS2Ab when the MOI was 100 plaque forming units (pfu)/*** expressed preS2Abs could recog-nize liver cells from HBV transgenic *** results showed that L02 cells expressing preS2Ab produced less HBsAg after treatment with the serum of HBV pa-tients than parental L02 cells expressing no *** transgenic mice treated with Ad315-preS2Ab had fewer and smaller cancerous nodes after induction with DEN than mice treated with a blank Ad315 vec-tor or untreated ***,the administration of Ad315-preS2Ab could alleviate hepatic cirrhosis and decrease the serum levels of alanine transaminase and aspartate ***:Adenovirus-mediated HBV preS2Ab expression could inhibit HBV infection in L02 cells,and then inhibit DEN-induced hepatocellular carcinogenesis and protect hepatic function in HBV transgenic mice.

关键词： Hepatitis B virus Adenoviral vector PreS2 antibody Hepatocellular carcinoma Gene therapy

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Effects of CpG oligodeoxynucleotide on transcription factors GATA-3 and T-bet mRNA expression in asthmatic mice

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Acta Pharmacologica Sinica 2005年第9期26卷 1117-1122页

作者： Zu-qun WU~(1,2) Yi-ping XU~3 Hua XIANG~4 Hua-hao SHEN~11 department of Respiratory Medicine,the second Affiliated hospital,4 department of Pathology,the First Affiliated hospital,School of Medicine,Zhejiang university,Hangzhou 310009,china 3 department of Respiratory Medicine,Renji hospital,shanghai second medical university shanghai 200001,china department of Respiratory Medicine the Second Affiliated Hospital Hangzhou China department of Respiratory Medicine Renji Hospital Shanghai Second Medical University Shanghai China department of Pathology the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou China

Aim:To investigate effects ofCpG oligod'eoxynucleotide(CpG ODN)on the mRNAexpression of transcription factors GATA binding protein 3(GATA-3)and T-boxexpressed in T cells(T-bet)in asthmatic ***:An asthmatic mousemodel was established and treated with CpG *** inflammatory cells andeosinophils in bronchoalveolar lavage fluid(BALF)were counted and inflamma-tory cell infiltration in lung tissue was ***-y and interleukin-4concentrations in BALF and splenocyte culture supernatants were detected us-ing an enzyme-linked immunosorbent *** factor GATA-3 and T-bet mRNA expression in splenocytes and lung tissue were detected by reversetranscription-polymerase chain ***:Total inflammatory cells andeosinophils in BALF were reduced in the CpG ODN-treated group compared withthe asthma group,and inflammatory cell infiltration in lung tissue was also signifi-cantly *** ODN treatment increased the interferon-γ concentrationbut decreased the interleukin-4 concentration in both BALF and splenocyte cul-ture ***-3 mRNA expression was reduced in both lung tissueand splenocytes in the CpG ODN-treated group,while the mRNA ratio ofT-bet toGATA-3 in splenocytes was ***:CpG ODN treatment inhibitsairway inflammatory cell infiltration and regulates interferon-y/interleukin-4 syn-thesis in asthmatic mice,possibly through a mechanism of downregulation ofGATA-3 mRNA expression in both lung tissue and splenocytes.

关键词： asthma inflammation CpG oligodeoxy-nucleotide GATA-3 T-bet mice

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The expression and antigenicity identification of recombinant rat TGF-β1 in bacteria

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Cell Research 2001年第2期11卷 95-100页

作者： GAO CHUN FANG, XIAN TAO KONG, AXEL M GRESSNER, RALF WEISKIRCHEN department of Laboratory Medicine, changzheng hospital, second military medical university, shanghai 200003, china Institute of Clinical Chemistry and Pathobiochemistry, Central Laboratory, R department of Laboratory Medicine Changzheng Hospital Second Military Medical University Shanghai China Institute of Clinical Chemistry and Pathobiochemistry Central Laboratory RWTH-University Hospital Aachen Germany

In order to study structure-function details of TGF-β1, the recombinant mature form of rat TGF-β1 was expressed in bacteria. Synthesis of the 112 amino-acid carboxyl-terminal part of TGF-β1 (amino acid 279-390) was controlled by an inducible gene expression system based on bacteriophage T7 RNA polymerase. This system allowed an active and selective synthesis of recombinant TGF-β1. The molecular weight of expressed TGF-β1 monomer determined on SDS-polyacrylamide gel under reducing conditions was about 13 kD. Serial detergent washes combined with a single gel-filtration purification step were sufficient to purify the expression product to homogeneity. Amino-terminal sequencing revealed that the N-terminal of the recombinant protein was identical to the published data. In Western blot analysis the recombinant polypeptide showed excellent antigenicity against polyclonal TGF-β1 antibody. The mature recombinant rat TGF-β1 expressed in this study provides a useful tool for future detailed structural and functional studies.

关键词： TGF-β1 recombinant expression inclusion bodies purification antigenicity.

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Involvement of GABA and opioid peptide receptors in sevoflurane-induced antinociception in rat spinal cord

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Acta Pharmacologica Sinica 2005年第9期26卷 1045-1048页

作者： Ying-wei WANG~1 Xiao-ming DENG~2 Xin-min YOU~1 Shu-xiao LIU~2 Zhi-qi ZHAO~31 department of Anesthesiology,Xinhua hospital,shanghai second medical university,shanghai 200092,china 2 department of Anesthesiology,Changhai hospital,second military medical university shanghai 200433,china 3 Institute of Neurobiology,Fudan university,shanghai 200433,china department of Anesthesiology Xinhua Hospital Shanghai Second Medical University Shanghai China department of Anesthesiology Changhai Hospital Second Military Medical University Shanghai China Institute of Neurobiology Fudan University Shanghai China

Aim:The spinal cord is pivotal in immobility induced by volatile anestheticsbecause the anesthetics depress the activity of motor neurons in the spinal *** aim of this study was to observe the effects of sevoflurane on pain process-ing at the spinal ***:The firing of the gastrocnemius muscle wasevoked by electrical stimulation to the ipsilateral hindpaw in *** nociceptiveC response of electromyography（EMG）was selected to *** GABAAreceptor antagonist bicuculline（0.1 mg/kg）and opioid receptor antagonist nalo-xone（0.4 mg/kg）were administered intravenously,either in the presence or in theabsence of 1.0% ***:In rats with transected spinal cord,sevoflurane produced a profound reduction in the C response in a dose-and time-dependent *** the presence of 1.0% sevoflurane,the C responses wereincreased after injections of bicuculline and ***:Sevofluraneis a volatile anesthetic that acts directly on the spinal cord to suppress the nocice-ptive *** sevoflurane-induced suppression of the C response is antago-nized by either bicuculline or *** results suggest that spinal GABAAreceptors and opioid peptide receptors are involved in the sevoflurane-inducedsuppression of spinal nociception.

关键词： sevoflurane spinal cord pain measurement GABA receptors opioid receptors

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Complete resection of isolated pancreatic metastatic melanoma:A case report and review of the literature

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World Journal of Gastroenterology 2010年第36期16卷 4621-4624页

作者： Miao-Xia He,Hui Jiang,Jian-Ming Zheng,department of Pathology,Changhai hospital,second military medical university,shanghai 200433,china Bin Song,Xian-Gui Hu,Yi-Jie Zhang,department of General Surgery,Changhai hospital,second military medical university,shanghai 200433,china department of Pathology Changhai Hospital Second Military Medical University Shanghai 200433 China.

Isolated metastatic melanoma of the pancreas is very ***,there is very limited experience with surgical resection of pancreatic *** potential benefit of metastasectomy can improve the quality of life and survival time of *** present a case of a 39-year-old Chinese male with a solitary pancreatic tumor which was considered a cystic benign lesion for *** and immunohistochemistry showed that the tumor in pancreatic tail was a metastasis from a malignant melanoma of the *** other metastastic foci were found in *** tumor was completely resected with combined distal pancreatectomy and *** patient has survived 25 mo without any signs of local recurrence or other metastatic lesions after operation,indicating that complete surgical resection of a solitary metastatic melanoma of the pancreas can prolong the survival time of patients.

关键词： Melanoma Metastasis Pancreas Solitary tumor Resection

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