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Adenovirus-mediated expression of both antisense ODC and AdoMetDC inhibited colorectal cancer cell growth in vitro

Acta Pharmacologica Sinica 2006年第3期27卷 353-359页

作者： Bing ZHANG~2 Xian-xi LIU~(2,5) Yan ZHANG~2 Chun-ying JIANG~3 Qing-shan TENG~4 Hai-yan HU~2 Wei WANG~2 Lei GONG~2 ~2Institute of Biochemistry and Molecular Biology,School of Medicine.shandong university,jinan 250012,china ~3department of Coloproctology,Affiliated hospital of shandong university of Traditional Chinese Medicine,jinan 250014,china,~4department of Neurosurgery.Cleveland Clinic Foundation,Cleveland,OH 44195,USA Institute of Biochemistry and Molecular Biology School of Medicine Shandong University Jinan China department of Colo-proctology Affiliated Hospital of Shandong University of Traditional Chinese Medicine Jinan China department of Neurosurgery Cleveland Clinic Foundation Cleveland USA

Aim:To construct a recombinant adenovirus that can simultaneously expressboth antisense ornithine decarboxylase(ODC)and adenosylmethionine decar-boxylase(AdoMetDC)and detect its inhibitory effect on the intracellular polyaminepool and colorectal cancer cell ***:A 205-bp cDNA of AdoMetDCwas reverse-inserted into recombinant pAdTrack-ODCas vectors and recombinedwith pAdEasy-1 vectors in AdEasy-1 *** clones were selected andtransfected into the packaging cell HEK293 after they were linearized by *** fluorescent protein expression was used to monitor the process of adenovi-rus *** ODC and AdoMetDC protein levels were identified by west-ern blotting,and intracellular polyamine content was detected by reverse-phasehigh performance liquid chromatography.A viable cell count was used to deter-mine the growth of HT-29 cells with or without exogenous ***:Sequencing confirmed that AdoMetDC cDNA was successfully ligated into thepAdTrack-ODCas *** expression in 293 cells during virus packing andamplification was observed by fluorescence *** blotting dem-onstrated that both ODC and AdoMetDC were downregulated by Ad-ODC-AdoMetDCas,and consequently 3 kinds of polyamine(putrescine,spermidineand spermine)were reduced to very low ***-29 cell growth was signifi-cantly inhibited as compared with control conditions,and growth arrest was notreversed by exogenous ***:The successfully constructedrecombinant adenovirus,Ad-ODC-AdoMetDCas,blocked polyamine synthesisand has therapeutic potential for treating colorectal cancer in vitro.

关键词： polyamine ornithine decarboxylase adenosylmethionine decarboxylase adenovirus colorectal cancer

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Fungal thoracic vertebral osteomyelitis with epidural abscess causing spinal compression in a patient with history of lung inflammatory pseudotumor and brain cysticercosis

Fungal thoracic vertebral osteomyelitis with epidural absces...

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中华医学会神经外科学分会第九次学术会议

作者： Jiang zheng1+,wang yunyan1+,li gang1,Meng bin2,li chuanfu3,li xingang1~* 1 department of Neurosurgery,qilu hospital of shandong university,jinan 250012,china 2 department of Pathology,qilu hospital of shandong university,jinan 250012,china 3 department of Radiology,qilu hospital of shandong university,jinan 250012,china

Backgroud:Fungal thoracic vertebral osteomyelitis is rare,particularly with epidural abscess causing spinal *** recent literatures show an increasing trend in the number of cases being reported.

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Population pharmacokinetic model of valproate and prediction of valproate serum concentrations in children with epilepsy

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Acta Pharmacologica Sinica 2004年第12期25卷 10-17页

作者： De-chun JIANG, Li WANG2 department of pediatrics, Peking university First hospital, TherapeuticDrug Monitoring and Clinical Toxicology, Center of Peking university, Beijing 100034, china department of pediatrics Peking University First Hospital Therapeutic Drug Monitoring and Clinical Toxicology Center of Peking University 北京 100034

AIM: Using sparse data of valproate (VPA) serum concentrations to build a population pharmacokinetic (PPK) model of VPA in Chinese children with epilepsy and to predict serum concentrations for new patients using a Bayesian approach. METHODS: Two hundred epileptic children, whose VPA serum concentrations were collected, were divided randomly into two groups (A and B, n=100 each). The PPK parameter values of group A were calculated to establish a PPK Model by using the NPEM Program of USC*PACK software. Based on it, VPA serum concentrations of group B were predicted with the Bayesian Fitting Program of the USC*PACK software. To assess the accuracy and precision of prediction, a paired-comparisons t-test was run between predicted and ob- served concentrations, and then the mean prediction error (MPE), mean square prediction error (MSPE), root mean square prediction error (RMSPE), and coincidence rates for different percentages of prediction error were all calculated. RESULTS: Optimum PPK parameters were: Ka, 2.522±2.743 h-1; Vs, 0.329±0.496 L/kg; and Kel, 0.0438±0.0384 h-1. For group B, there was no significant difference between predicted and observed concentrations. MPE was -0.43 mg/L, MSPE was 115.40 (mg/L)2, and RMSPE was 5.47 mg/L. The coincidence rates for percent- ages of prediction error, which were less than 5 %, 10 %, 15 %, 20 %, 25 %, and 30 %, were 62 %, 74 %, 82 %, 85 %, 89 %, and 93 %, respectively. CONCLUSION: A PPK model of VPA in epileptic children was successfully established. Based on it, VPA serum concentrations can be predicted accurately with a Bayesian approach.

关键词： pharmacokinetics valproic acid epilepsy Bayes theorem USC*PACK software

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Structure-efficacy relationships of immunostimulatory activity of CpG-containing oligodeoxynucleotides on mouse spleen cells

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Acta Pharmacologica Sinica 2007年第10期28卷 1637-1644页

作者： Hai-yan DU~3,Shao-you XIA~4,Hai-feng SONG~(2,5),Na LI~2,Ming-mei SHANG~2,Jia ZOU~2,Qing-qing WANG~2,Lun OU~2,XiaoSUN~2,Ai-guo JI~3,Zhong-ming TANG~2~2 department of Pharmacology and Toxicology,Beijing Institute of Radiation Medicine,Beijing 100850,china ~3 Institute of Biochemical andBiotech Drugs,School of Pharmacy,shandong university,Ji-nan 250012,china ~4 department of General Surgery,General hospital of PLA,Beijing100853,china department of Pharmacology and Toxicology Beijing Institute of Radiation Medicine Beijing 100850 China. songhf@***.

Aim:To study the relationship between primary structures of oligodeoxynucleo-tides （ODN） containing unmethylated deoxycytidyl-deoxyguanosine （CpG）dinucleotide motifs and their immunostimulatory activities in mouse spleen ***:A series of CpG ODN with different primary structures were *** capabilities to stimulate mouse spleen cell proliferation were determined by[3H]thymidine incorporation *** （interleukin [IL]-6,IL-12,and IFN-α）secretion spectra induced by CpG ODN were assessed by *** ability ofCpG ODN to activate natural killer cells was evaluated by standard 4 *** cytometry was utilized to examine the expressions of various lympho-cyte surface molecules on diverse *** effective CpG ODN for murine,ODN1826,was set as the template of modification and the positive ***:The immunostimulatory activities of CpG ODN with different sequencesand compositions varied markedly,both in character and in *** was uselessfor improving the immunostimulatory activity of ODN1826 by simply increasingthe functional hexameric CpG motif number,modifying the site of CpG motifs,orchanging the distance between multi-CpG ***,an addition of a self-complementary palindrome structure at the 3'-end,but not the 5'-end of CpG ODN,aroused marked improvement in its *** designed ODN had superiorcomprehensive immunostimulatory properties compared to ODN ***:The immunostimulatory activity of a CpG ODN was relevant to its primary *** was useless for promoting immunostimulatory activity to simply change CpGmotif number,space,or *** 3'-end palindrome structure of CpG ODN isassociated with enhanced immunostimulatory activity.

关键词： CpG oligodeoxynucleotides immunostimulation structure-activity relationship

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The Expression of Programmed Cell Death 4 and Survivin in Condyloma Acuminatum and Bowenoid Papulosis

The Expression of Programmed Cell Death 4 and Survivin in Co...

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The 10th china-Japan Joint Meeting of Dermatology 20th Anniversary

作者： Shijing Chen Yunkun Wang Shuping Yu department of Dermatology qilu hospital of shandong university jinan 250012 china

＜正＞Objective:To explore:*** expression of the Programmed Cell Dead Factor in condyloma acuminatum and bowenoid papulosis;*** expression of the Survivin in condyloma acuminatum and bowenoid papulosis.

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Population pharmacokinetics of valproate in Chinese children with epilepsy

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Acta Pharmacologica Sinica 2007年第10期28卷 1677-1684页

作者： De-chun JIANG~(2,3),Li WANG~4,Yu-qin WANG~(2,6),Lin LI~3,Wei LU~5,Xiang-rong BAI~2~2 department of Pharmacy,Xuan-wu hospital of Capital Medical university,Beijing 100053,china ~4 department of pediatrics,Peking universityFirst hospital,Beijing 100034,china ~3 department of Pharmacology,Xuan-wu hospital of Capital Medical university,Education Ministry KeyLaboratory for Neurodegenerative Diseases,Beijing 100053,china ~5 School of Pharmaceutical Science,Peking university,Beijing 100083,china department of Pharmacy Xuan-wu Hospital of Capital Medical University Beijing China department of Pharmacology Xuan-wu Hospital of Capital Medical University Education Ministry Key Laboratory for Neurodegenerative Diseases Beijing China department of pediatrics Peking University First Hospital Beijing China School of Pharmaceutical Science Peking University Beijing China

Aim:The aim of the present study is to establish a population pharmacokinetic（PPK） model of valproate （VPA） in Chinese epileptic children to promote thereasonable use of anti-epileptic ***:Sparse data of VPA serumconcentrations from 417 epileptic children were *** patients weredivided into 2 groups:the PPK model group （n=317） and the PPK valid group（n=100）.The PPK parameter values of VPA were calculated by NONMEM soft-ware using the data of the PPK model group.A basic model and a final modelwere set *** validate the 2 models,the concentrations of PPK valid groupwere predicted by each model,*** mean prediction error （MPE）,mean squared prediction error （MSPE）,root mean squared prediction error（RMSPE）,weight residues （WRES）,and the 95% confidence intervals （95%CI） were also ***,the values between the 2 models were ***:The PPK of VPA was determined by a 1-compartment model with afirst-order absorption *** basic model was:Ka=3.09 (h-1),V/F=20.4（L）,CL/F=0.296 （L/h）.The final model was:Ka=0.251+2.24·（1-HS）(h-1),V/F=2.88+0.157·WT （L）,CL/F=0.1060.98·CO+0.0157·AGE （L/h）.For thebasic model,the MPE,MSPE,RMSPE,WRES,and the 95% CI were-23.53（-30.36,-16.70）,3728.96 （2872.72,4585.20）,39.62 （34.34,44.90）,and-0.06 （-0.14,0.02）,*** the final model,the MPE,MSPE,RMSPE,WRES,and the95% CI were-1.16 （-4.85,2.53）,1002.83 （1050.64,1143.61）,23.04 （21.12,24.96）,and0.08 （-0.04,0.20）,*** final model was more optimal than the ***:The PPK model of VPA in Chinese epileptic children wassuccessfully *** will be valuable to facilitate individualized dosageregimens.

关键词： population pharmacokinetics valproic acid epilepsy children Bayesian method NONMEM software individualized dosage regimens

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实时三平面应变率评价冠状动脉搭桥术前后左室长轴局部收缩功能

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中国超声医学杂志 2012年第6期28卷 563-566页

作者：李荣卜培莉山东大学齐鲁医院心内科

目的探讨实时三平面应变率成像技术评价非体外循环冠状动脉搭桥术(OPCABG前后左室长轴局部收缩功能的临床应用价值。方法应用实时三平面应变率成像技术检测OPCABG前后室间隔及左室壁各节段收缩期峰值应变率(SRS)。结果与术前比较,OPCAB... 详细信息

目的探讨实时三平面应变率成像技术评价非体外循环冠状动脉搭桥术(OPCABG前后左室长轴局部收缩功能的临床应用价值。方法应用实时三平面应变率成像技术检测OPCABG前后室间隔及左室壁各节段收缩期峰值应变率(SRS)。结果与术前比较,OPCABG术后1周部分节段SRS增加(P3个月各节段SR_s进一步增加(P<0.05)。结论 OPCABG后,左室长轴局部收缩功能明显改善;实时二平面应变率成像技术可以定量准确评价OPCABG前后左室长轴局部收缩功能的变化。

关键词：冠心病非体外循环冠状动脉搭桥术左室长轴局部收缩功能实时三平面应变率成像

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A mutation in signal peptide of rat resistin gene inhibits differentiation of 3T3-L1 preadipocytes

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Acta Pharmacologica Sinica 2004年第12期25卷 139-145页

作者： Xi-rong GUO2,3, Hai-xia GONG2, Yan-qin GAO4, Li FEI, Yu-hui NI, Rong-hua CHEN department of pediatrics, Center of Human Functional Genomics, Nanjing Medical university, Nanjing 210029, china 4department of pediatrics, the Third Affiliated hospital of Nanjing Medical university, Yizheng 211900, china department of pediatrics Center of Human Functional Genomics Nanjing Medical University Nanjing 210029 department of pediatrics the Third Affiliated Hospital of Nanjing Medical University Yizheng 211900

AIM: To detect the resistin expression of white adipose tissue in diet-induced obese (DIO) versus diet-resistant (DR)rats, andto investigatethe relationshipof mutatedresistin and 3T3-L1 preadipocytes differentiation. METHODS: RT-PCR and Western Blot were used to detect gene /protein expression. 3T3-L1 cells were cultured, transfected, and induced to differentiation using 0.5 mmol/L 3-isobutyl-1-methyxanthine (MIX), 1 mg/L insulin, and 1 μmol/L dexamethasone. Oil red O staining was applied to detect the degree of preadipocytes differentiation. RESULTS: Expression of resistin mRNA was upregulated in DIO rats and downregulated in DR rats. However, the expression levels varied greatly within the groups. Sequencing of the resistin genes from DIO and DR rats revealed a Leu9Val (C25G) missense mutation within the signal peptide in one DR rat. The mutant resistin inhibited preadipocyte differentiation. Local experiments and Western blotting with tagged resistin fusion proteins identified both mutant and wild type proteins in the cytoplasm and secreted into the culture medium. Computer predictions using the Proscan and Subloc programs revealed four putative phosphorylation sites and a possible leucine zipper motif within the rat resistin protein. CONCLUSION: Resistin-increased differentiation may be inhibited by the mutation- containing precursor protein, or by the mutant non-secretory resistin isoform.

关键词： resistin mutation adipocyte differentiation obesity

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Prolonged exposure to resistin inhibits glucose uptake in rat skeletal muscles

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Acta Pharmacologica Sinica 2007年第3期28卷 410-416页

作者： Hong-qi FAN~(2,3) Nan GU~(2,3) Feng LIU~2 Li EEI~2 Xiao-qin PAN~2 Mei GUO~2 Rong-hua CHEN~(2,4) Xi-rong GUO~(2,3,4)2 department of pediatrics,Nanjing Maternal and Child Health hospital of Nanjing Medical university,Nanjing 210004,china 3 Institute of pediatrics Medicine,Nanjing Medical university,Nanjing 210029,china department of pediatrics Nanjing Maternal and Child Health Hospital of Nanjing Medical University Nanjing China Institute of pediatrics Medicine Nanjing Medical University Nanjing China

Aim:To assess the effects and mechanisms of the action of resistin on basal andinsulin-stimulated glucose uptake in rat skeletal muscle ***:Rat myo-blasts（L6）were cultured and differentiated into myotubes followed by stimula-tion with single commercial resistin（130 ng/mL,0-24 h）or cultured supernatantfrom 293-T cells transfected with resistin-expressing vectors（130 ng/mL,0-24 h）.Liquid scintillation counting was used to quantitate[3H]2-deoxyglucose *** translocation of insulin-sensitive glucose transporters GLUT4 and GLUT1,synaptosomal-associated protein 23（SNAP23）and GLUT protein content,as wellas the tyrosine phosphorylation status and protein content of insulin receptorsubstrate（IRS）-1,were assessed by Western ***:Treatment of L6myotubes with single resistin or cultured supernatant containing recombinantresistin reduced basal and insulin-stimulated 2-deoxyglucose uptake and impairedinsulin-stimulated GLUT4 *** SNAP23 protein content wasdecreased,no effects were noted in GLUT4 or GLUT1 protein *** diminished insulin-stimulated IRS-1 tyrosine phosphorylation levels withoutaffecting its protein *** effects of recombinant resistin from 293-T cellstransfected with resistin-expressing vectors were greater than that of single ***:Resistin regulated IRS-1 function and decreased GLUT4translocation and glucose uptake in response to *** downregulatedexpression of SNAP23 may have been partly attributed to the decrease of glucoseuptake by resistin *** observations highlight the potential role ofresistin in the pathophysiology of type 2 diabetes related to obesity.

关键词： resistin insulin glucose uptake obesity diabetes

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Interaction between hydrogen sulfide/cystathionine γ-lyase and carbon monoxide/heme oxygenase pathways in aortic smooth muscle cells

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Acta Pharmacologica Sinica 2006年第12期27卷 1561-1566页

作者： Hong-fang JIN~2 Jun-bao DU~(2,4)Xiao-hui LI~2 Yan-fei WANG~2 Yin-fang LIANG~2 Chao-shu TANG~3 2 department of pediatrics,Peking university First hospital,Beijing 100034,china 3 Institute of Cardiovascular Research,Peking university First hospital,Beijing 100034,china department of pediatrics Peking University First Hospital Beijing China Institute of Cardiovascular Research Peking University First Hospital Beijing China

Aim:To investigate the interaction between hydrogen sulfide（H2S）/cystathion-ine γ-lyase（CSE）and carbon monoxide（CO）/heme oxygenase（HO）pathways inaortic smooth muscle cells（ASMC）.Methods:The ASMCs were divided into thefollowing groups:（1）the control group;（2）the zinc protoporphyrin（ZnPP）20μmol/L group;（3）the propargylglycine（PPG）2 mmol/L,4 mmol/L and 10 mmol/Lgroups;and（4）the sodium hydrosulfide（NariS）1×10-5mol/L,1×10-4mol/L and1×10-3mol/L *** of the groups was further divided into 6h,12h,18hand 24h *** CO level,represented by carboxyhemoglobin（HbCO）content was measured using a spectrophotometric method and H2S content wasdetected by a sensitive electrode *** and HO-1 expressions were de-tected by Western ***:The H2S content in the medium and CSEexpression by ASMC were markedly increased by ZnPP compared with the con-trol *** content in the medium and HO-1 expression by the ASMCstarted strengthening following 24h treatment with PPG at 2 mmol/L,but werefurther strengthened following 18h and 24h treatment with PPG at 4 mmol/Lcompared with the controls（P3mol/L decreased them at all timepoints of the ***:The results suggested that endogenousCO/HO and H2S/CSE pathways inhibited each other in ASMC under physiologi-cal conditions.

关键词： hydrogen sulfide cystathionine γ-lyase carbon monoxide heme oxygenase

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