检索结果-南通市图书馆

肺癌细胞高、低转移株中uPA基因启动子的序列和活性

在线全文

cnki会议

学校读者我要写书评

暂无评论

复旦学报（医学版） 2004年第3期31卷 243-246页

作者：王丽石李平谭理朱运松复旦大学上海医学院生物化学与分子生物学系-教育部分子医学重点实验室上海200032

目的　分析人肺巨细胞癌高、低转移株中uPA基因启动子序列差异和这种差异对启动子活性的影响。方法　用PCR分段扩增人肺巨细胞癌高 (95D)和低 (95C)转移细胞株中uPA基因启动子序列 ,将uPA启动子亚克隆到荧光素酶为报告基因的 pGL3 Basi... 详细信息

目的　分析人肺巨细胞癌高、低转移株中uPA基因启动子序列差异和这种差异对启动子活性的影响。方法　用PCR分段扩增人肺巨细胞癌高 (95D)和低 (95C)转移细胞株中uPA基因启动子序列 ,将uPA启动子亚克隆到荧光素酶为报告基因的 pGL3 Basic载体上 ,转染 95D或 95C细胞 ,分析启动子活性。结果　 95D与 95C细胞uPA基因启动子序列中有 3个位点碱基的差别 ,95D分别是 - 1933bp为“T” ,- 4 2.4bp为“C” ,- 96bp为“G” ,而 95C分别为“C”、“T”、“T”。这些不同对启动子活性的影响未见差别。在 - 2.14 3/ - 182.4之间存在uPA基因启动子的增强子序列 ,在 - 182.4 / - 4 0 8之间存在uPA启动子的负性调控序列。结论　uPA在 95D与 95C细胞中表达的差别不是直接由于启动子序列差别引起的。

关键词：肺癌转移株 uPA基因启动子序列活性癌细胞

大学英语分层教学中中等学生词汇学习认知分析

在线全文

学校读者我要写书评

暂无评论

山东外语教学 2009年第2期30卷 72-75页

作者：麻珍玉刘拴桂林工学院外语系广西桂林541004 桂林工学院博文管理学院外语系广西桂林541004

为掌握分层教学中中等学生词汇学习中的障碍与困难，提高词汇学习效率，本文用问卷调查和与相关学生访谈的形式，对某工科院校378名中等学生的词汇学习状况进行了调查。根据调查结果，对学生在词汇表背诵、词汇联想和围绕原型意义延伸... 详细信息

为掌握分层教学中中等学生词汇学习中的障碍与困难，提高词汇学习效率，本文用问卷调查和与相关学生访谈的形式，对某工科院校378名中等学生的词汇学习状况进行了调查。根据调查结果，对学生在词汇表背诵、词汇联想和围绕原型意义延伸词义三方面从认知语言学的角度进行了分析。通过分析，提出了注重外显知识的促进作用和提高围绕原型意义组词的能力的理论探讨。

关键词：分层词汇认知

c-Src/STAT3 signal is involved in EGF-induced proliferation of rat spermatogonial stem cells

在线全文

学校读者我要写书评

暂无评论

c-Src/STAT3 signal is involved in EGF-induced proliferation ...

“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Jia-xiang Chen~a,Lin-lin Xu~b,Jing-lei Wang~a a.department of Physiology,medical {2. of Nanchang university,Nanchang 330006,P.R.china. b.medical Rcscrch Center,The First Affiliated hospital of Nanchang university,Nanchang 330006,P.R.china.

Epidermal growth factor(EGF) plays a role in male germ cell development,but the precise function is yet to be *** present study shows that EGF stimulates rat spermatogonial proliferation in a dose-dependent manner and significantly increased the protein levels of phosphated c-Src(p-c-Src) and phosphated signal transducer and activator of transcription 3(p-STAT3).Moreover,over-expression of c-Src tagged with enhanced green fluorescence protein(EGFP) in rat spermatogonial stem cells enhances the cell *** contrast,knockdown or inhibition of c-Src inhibits rat spermatogonial stem cell proliferation;EGF could not abrogate the inhibitory ***,the content of p-STAT3 protein was increased in c-Src expressing cells and decreased in c-Src suppressing ***,knockdown or inhibition of STAT3 also suppressed cell viability;neither EGF nor increased c-Src could reverse the inhibitory *** results are the first evidence that EGF induces proliferation of rat spermatogonial stem cells via c-Src/STAT3 signal.

关键词： Epidermal growth factor spermatogonial stem cells c-Src STAT3

S_2._8^(2.)/ZrO_2.SiO_2.Sm_2._3固体超强酸催化剂的XPS研究

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国稀土学报 2003年第Z2期21卷 79-83页

作者：吴燕妮崔秀兰郭海福内蒙古工业大学化工学院内蒙古呼和浩特010062 肇庆学院轻化工系广东肇庆526061

采用XPS实验技术对不同制备条件下制得的S2.82./ZrO2.SiO2.Sm2.3类固体超强酸催化剂的表面元素电子结合能及表面元素的相对含量进行了分析对比。结果表明:浸渍S2.82.使得S2.,Zr3d结合能提高;加入少量氧化钐制得催化剂的S2.,Zr3d结合能... 详细信息

采用XPS实验技术对不同制备条件下制得的S2.82./ZrO2.SiO2.Sm2.3类固体超强酸催化剂的表面元素电子结合能及表面元素的相对含量进行了分析对比。结果表明:浸渍S2.82.使得S2.,Zr3d结合能提高;加入少量氧化钐制得催化剂的S2.,Zr3d结合能略大于不加稀土的样品,说明少量的稀土元素改变了催化剂表面的化学状态,使催化剂表面元素正极化程度提高;焙烧使得催化剂表面S,Zr原子作用力增强,金属原子的正极化程度增强。不同该类催化剂的Si2.结合能基本相同,说明S未与Si结合,二氧化硅不是该类催化剂的活性组份。

关键词：固体超强酸催化剂 XPS 稀土氧化物

Low dose and short-term therapy of tibolone reduces the cardiovascular disease risk in postmenopausal women

在线全文

学校读者我要写书评

暂无评论

生殖医学杂志 2004年第Z1期13卷 8-13页

作者： Ye Bi-lu Cheng Jing Ye Lian department of Reproductive Medicine the 1st Hospital of Wenzhou Medical College Wenzhou Zhejiang 325000 China Department of Reproductive Medicine the 1st Hospital of Wenzhou Medical College Wenzhou Zhejiang 325000 China Department of Reproductive Medicine the 1st Hospital of Wenzhou Medical College Wenzhou Zhejiang 325000 China department of Obstetrics & Gynecology Wayne State UniversityDetroit MI 48201USA

Objective:To investigate the effects of low dose tibolone short-term therapy on clinic, endocrine and markers of cardiovascular disease in healthy postmenopausal ***: A prospective study involved a total of 42.eligible postmenopausal women. 2. cases as group A and 2. cases as group B. Complete baseline work-up including Kupperman score,body mass index (BMI), gonadotropin (FSH, LH), estrogen (E2., testosterone (T), sex hormone binding globulin (SHBG), plasminogen activator inhibitor type 1 (PAI-1), tissue plasminogen activator (tPA), high-sensitivity C-response protein (hs-CRP), nitrogen oxide (NO)and fasting lipid, glucose(FPG), insulin(FINS) were performed in all subjects. Postmenopausal women in group A were treated with 1.2. mg tibolone daily. Women in group B were treated with 0. 62. mg tibolone daily. Women both in group A and group B were given calcium 600 mg with vitamin D 12.IU per day. At the end of the 12.weeks therapy, subjects were re-evaluated and above parameters were ***:No significant differences between group A and group B were found at ***-eight cases (fourteen cases in each group) completed the study. Kupperman score decreased from (2..1±8.0) and (2..4±7.5) to (7.7±4.5) and (5.2.4.5) and plasminogen activator inhibitor type 1 decreased from (95.8±32.4)μg/L and (102.9±42.6)μg/L to (72.2.39.6)μg/L and (79.9±30. 1) μg/L significantly in group A and group B respectively after treatment. In group A, Blood pressure decreased significantly from (12. ± 10)/(83 ± 6) mmHg to (110±14)/(77± 9) mmHg (P＜0.05), testosterone increased significantly from (0. 6 ±0. 4)nmol/L to (1.3 ± 1.1) nmol/L (P＜0. 05), free testosterone increased from (0. 001 ±0. 002.nmol/L to (0. 003±0. 003) nmol/L significantly (P＜0.01), SHBG decreased from (7.6±4. 9)nmol/L to (4. 3±2.9) nmol/L significantly (P＜0.05), total cholesterol decreased from (5.4±0. 8) mmol/L to (5.0±0.8) mmol/L significantly (P＜0.01), ApoA decreased from (1.8±0.3)mg/dl to (1.7±0. 3

关键词： Postmenopausal women Hormone replacement therapy Tibolone Cardiovascular effects

Research on Manufacturing technology Based on Machine Vision

在线全文

学校读者我要写书评

暂无评论

武汉理工大学学报 2006年第S2期28卷 402-406页

作者： HU Zhanqi ZHENG Kuijing (Mechanical Engineering college,Yanshan university,Qinhuangdao 066004,{2. Mechanical Engineering college Yanshan UniversityQinhuangdao 066004China

The concept of machine vision based manufacturing technology is proposed first,and the key algorithms used in two-dimensional and three-dimensional machining are discussed in *** information can be derived from the binary images and gray picture after processing and transforming the *** and the parallel cutting method about two-dimen- sional machining are *** approximating algorithm is used to cutting the profile of the *** Scanning al- gorithm used to machining inner part of a *** improved Shape From Shading method with adaptive pre-processing is adopted to reconstruct the three-dimensional *** cutting method is adopted for three-dimensional *** tool path is then gotten from the model,and NC code is formed *** model can be machined conveniently by the lathe, milling machine or *** examples are given to demonstrate the results of lmageCAM system,which is developed by the author to implement the algorithms previously mentioned.

关键词： index terms-machine vision manufacturing technology image processing

Chiral discrimination of alanine enantiomers in N+H…O modes by polarized Raman vibration spectroscopy with b(cc)b geometry

在线全文

学校读者我要写书评

暂无评论

Chiral discrimination of alanine enantiomers in N+H…O modes...

第一届全国生物物理化学会议暨生物物理化学发展战略研讨会

作者： Yan Gong~(*1),Yongkai Lu~2.and Wenqing Wang~3 1 Beijing Key Laboratory of Clothing Materials R.& D and Assessment,School of Materials Science & Engineering,Beijing Institute of Fashion technology,Beijing 10002.,china 2.Medicine School,Tsinghua {2.,Beijing 100084,china 3 department of Applied Chemistry,Beijing National Laboratory for Molecular Sciences,college of Chemistry,Peking {2.,Beijing 100871,china

Chirality breaking of D- and L-alanine crystals in the c-direction of(NH…O) modes was observed by polarized Raman spectroscopy with b(cc)b geometry at *** to the Raman effects with vibrational states in the process of scattering alanine crystal,the ups and downs of(NH…O) hydrogen bonding showed strong directionality in alanine *** study reveals an intrinsic chirality in

关键词： chiral discrimination alanine enantiomers N+H...O mode raman spectroscopy

The Mitochondrial Genome of Baylisascaris procyonis

在线全文

cnki会议

学校读者我要写书评

暂无评论

The Mitochondrial Genome of Baylisascaris procyonis

中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十一次学术研讨会

作者： Xie Yue~1,Zhang Zhihe~2.Niu Lili~3,Wang Qiang~3,Wang Chengdong~2.Lan Jingchao~2.Deng Jiabo~3, Fu Yan~1,Nie Huaming~1,Yan Ning~1,Yang Deying~1,Hao Guiying~1,Gu Xiaobin~1,Wang Shuxian~1, Peng Xuerong~4,Yang Guangyou~(1*) 1.department of Parasitology,college of Veterinary Medicine,Sichuan Agricultural {2.,Ya’an 62.014,china. 2.The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife-Developing toward a State Key Laboratory for china,Chengdu Research Base of Giant Panda Breeding,Chengdu,Sichuan 610081,china 3.Chengdu Zoological Garden,Chengdu,Sichuan 610081,china 4.department of Chemistry,college of Life and Basic Science,Sichuan Agricultural {2.,Ya’an 62.014,china.

Baylisascaris procyonis(Ascaridida:Nematoda),an intestinal nematode of raccoons,can cause serious or fatal larval migrans in numerous species of birds and mammals,including *** its significant impact in veterinary and public health,the epidemiology,molecular ecology and population genetics of this parasite remain largely *** genomes can provide a foundation for investigations in these areas and assist in the diagnosis and control of *** this study,the first complete mt genome sequence of *** was determined using polymerase chain reaction(PCR) combined with a primer-walking *** circular mapping 14781 bp mt genome of *** contained 12.protein-coding,2. transfer RNA,and 2.ribosomal RNA genes congruent with other chromadorean ***,the *** mtDNA featured an extremely long AT-rich region (1375 bp) and a high number of intergenic spacers(17),which make it significantly different from those of other secernentean nematodes characterized to ***,the entire genome displayed notable levels of AT skew and GC *** on pairwise comparison and sliding window analysis within and between mt genes among the available 11 Ascaridida mtDNAs,cox1 and cox2.were determined to be the most conserved protein-coding genes,while cytb and nad4 were the least conserved *** primer pairs designed to amplify species-specific short fragments of *** mtDNA were developed for the genes cytb(548 bp fragment) and rmL(2.0 bp fragment),and tested as possible alternatives to existing mt markers for *** complete mt genome sequence of *** sequenced here is expected to contribute to molecular diagnostic methods,epidemiological investigations and ecological studies of B. procyonis and other related ascarids,and provide a rich resource of markers for systematic,population genetic and evolutionary biological studies of parasitic nematodes of socio-economic importance.

关键词：

Real-Time Music Synthesis by Patch-Based Method

在线全文

cnki会议

学校读者我要写书评

暂无评论

Real-Time Music Synthesis by Patch-Based Method

第二十九届中国控制会议

作者： ZHU Bin 1,WANG Xin 2.1.college of information science of technology,Zhejiang Shuren {2.,Hangzhou 310015,P.R.china2.college of Computer Science and technology,Zhejiang {2. of technology,Hangzhou 31002.,P.R.china

This page proposed a new method to synthesize arbitrary length music sequences from a small database of music clips in *** method actually is a patch-base method which is used commonly in texture synthesis of image or motion capture *** extracted the music patches automatically from each music clips of the training *** music patch consisted of three parts:head,body and *** the head part and the trail part were represented by the n-gram pattern feature *** increase the speed of patch matching process,we employed the K-D tree structure to store the feature vectors due to its efficiency and accuracy of multi-dimension *** results showed the proposed method is especially useful for interactive and real-time application such as background music generation for games and broadcasting.

关键词： Music analysis Music synthesis Music segmentation Music retrieval Pattern recognition