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Prevention of pathological change and cognitive degeneration of Tg2.76 mice by inoculating Aβ_(1-15) vaccine

Science china(Life Sciences) 2008年第8期51卷 743-750页

作者： HU JinJia1, LI GuoYing2. WANG HuaQiao3, LIN Xian3 & YAO ZhiBin3 1 department of anatomy, Medical College of Shanghai Jiao Tong {2., Shanghai 2.002., china 2.department of anatomy, Preclinical College, Guangdong {2. of Pharmacy, Guangzhou 510006, china 3 department of anatomy, zhongshan school of medicine, sun yat-sen university, Guangzhou 510080, china 1. department of anatomy Medical College of Shanghai Jiao Tong University Shanghai 200025 China 2. department of anatomy Preclinical College Guangdong University of Pharmacy Guangzhou 510006 China 3. department of anatomy Zhongshan School of Medicine Sun Yat-sen University Guangzhou 510080 China

This study aims to discuss the effect of preventing pathological changes and cognitive degeneration of Tg2.76 mice by inoculating the subunit fragment of Aβ ***-two Tg2.76 mice were randomly divided into four groups, each having eight mice:Group I, the control group, inoculated with adjuvants;Group II, the Aβ42.group, inoculated with Aβ42.vaccine;Group Ⅲ, the Aβ1―15 group, inoculated with Aβ1―15 vaccine;and Group IV, the Aβ36―42.group, inoculated with Aβ36―42.*** titer of the serum anti-body against Aβ42.Group II) was significantly higher than that of the control group(Group I), and a low level of antibodies could be detected in the brain homogenate in the three vaccine-inoculated *** water maze test showed that the Aβ42.group, Aβ1―15 group and Aβ36―42.group were obviously im-proved compared with the control *** cultured splenocytes sampled from each group were induced by Con A or their respective antigens, and the cell proliferation of the three vaccine-inoculated groups was significantly higher than that of the control *** the Aβ42.group, IL2.and IFN-γ were relatively low and IL4 and IL10 were relatively *** contrast, IL4 and IL10 were much higher in the Aβ1―15 group and IL2.and IFN-γ were much higher in the Aβ36―42.*** immunohistochemical test showed a large number of senile plaques in the brain cortex and hippocampus of the mice in the con-trol group, no senile plaque in the brain of the Aβ1―15 group and Aβ42.group mice, and a small number of senile plaques in the brain of the Aβ36―42.group *** results suggest that the subunit fragment of Aβ1―15 vaccine could prevent not only cognitive and behavioral degeneration but also Aβ deposition and formation of senile plaques in Tg2.76 mice.

关键词： Alzheimer’s disease β-amyloid peptide vaccine Tg2.76 mice senile plaque

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Non-invasive Prenatal Gene Diagnosis: Progress through Cell-free Fetal DNA and RNA in Maternal Plasma and Urine

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中山大学学报（医学科学版） 2008年第S2期29卷 140-142页

作者： GUO Xun-yang, GUO Yi-bin ( department of Medical Genetics, zhongshan school of medicine,{2., Guangzhou 510080, china )

Non-invasive prenatal gene diagnosis has been developed rapidly in the recent years, and numerous medical researchers are focusing on it. Such techniques could not only achieve prenatal diagnosis accurately, but also prevent tangential illness in fetuses and thus, reduce the incidence of diseases. Moreover, it is non-invasive prenatal gene diagnosis that prevents potential threaten and danger to both mothers and fetuses. Therefore, it is welcomed by clinical gynecologist and obstetrian, researchers of medical genetics, and especially, pregnancies. This review article touches briefly on the advanced development of using cell-free DNA, RNA in maternal plasma and urine for non-invasive prenatal gene diagnosis.

关键词： non-invasive prenatal gene diagnosis cell-free fetal DNA and RNA DNA and RNA detection maternal urine maternal plasma

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Interferon-α enhances sensitivity of human osteosarcoma U2.S cells to doxorubicin by p53-dependent apoptosis

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Acta Pharmacologica Sinica 2007年第11期28卷 1835-1841页

作者： Xiang-wei YUAN~2.Xiao-feng ZHU~(3,5),Xiu-fang HUANG~4,Pu-yi SHENG~2.Ai-shan HE~2.Zi-bo YANG~2.Rong DENG~3,Gong-kanFENG~3,Wei-ming LIAO~(2.5)~2.department of Orthopedic Surgery,The First Affiliated Hospital,sun {2.-sen university,Guangzhou 510080,china ~3 State Key Laboratoryof Oncology in South china,Cancer Center,sun {2.-sen university,Guangzhou 510060,china ~4 department of Pathology,The AffiliatedJiangmen Hospital,sun {2.-sen university,Jiangmen 52.030,china department of Orthopedic Surgery The First Affiliated Hospital Sun Yat-sen University Guangzhou 510080 China State Key Laboratory of Oncology in South china Cancer Center Sun Yat-sen University Guangzhou 510060 China department of Pathology The Affiliated Jiangmen Hospital Sun Yat-sen University Jiangmen 529030 China

Aim:To determine whether interferon-α(IFNα) can enhance doxorubicinsensitivity in osteosarcoma cells and its molecular ***:Cellviability was evaluated using 3-(4,5-dimethylthiazol-2.yl)-2.5-diphenyltetrazoliumbromide *** was studied using Flow cytometry analysis,Hoechst332.8 staining,DNA fragmentation assay,as well as the activation ofcaspase-3 and poly (ADP-ribose) *** expression was detectedby Western *** dependence of p53 was determined using ***:IFNα increased doxorubicin-induced cytotoxicity to a muchgreater degree through apoptosis in human osteosarcoma p53-wild U2.S cells,but not p53-mutant MG63 ***α markedly upregulated p53,Bax,Mdm2.andp2.,downregulated Bcl-2.and activated caspase-3 and PARP cleavage in re-sponse to doxorubicin in U2.S ***,the siRNA-mediated silencing ofp53 significantly reduced the IFNα/doxorubicin combination-induced cytotoxic-ity and PARP ***:IFNα enhances the sensitivity of humanosteosarcoma U2.S cells to doxorubicin by p53-dependent *** propercombination with IFNα and conventional chemotherapeutic agents may be a ra-tional strategy for improving the treatment of osteosarcoma with functional p53.

关键词： Interferon-α doxorubicin apoptosis p53 osteosarcoma

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High norbornene incorporation in ethylene-norbornene copolymerization with a bis(α-alkyloxoimine) titanium-MAO catalyst

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Science china Chemistry 2010年第8期53卷 1634-1640页

作者： GAO HaiYang1,2.HU Hao1 & WU Qing1,2.1Institute of Polymer Science,school of Chemistry and Chemical Engineering,sun {2.-sen (zhongshan) university,Guangzhou 5102.5,china 2.ey Laboratory for Polymeric Composite and Functional Materials of Ministry of Education sun {2.-sen (zhongshan) university,Guangzhou 5102.5,china 1. Institute of Polymer Science School of Chemistry and Chemical Engineering Sun Yat-Sen (Zhongshan) University Guangzhou 510275 China2. Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education Sun Yat-Sen (Zhongshan) University Guangzhou 510275 China

A novel bis(α-alkyloxoimine) titanium(IV) complex was synthesized and used as a catalyst precursor to catalyze homo-and copolymerization of ethylene and *** titanium complex activated with methylalumoxane exhibits good activities for the homopolymerizations of ethylene and norbornene under high temperature to produce high-molecular-weight linear polyethylene and vinyl-type polynorbornene,***-norbornene copolymers with high molecular weight can also be produced by this *** incorporation of norbornene from 0 to 76 mol% in the copolymers can be controlled by varying the charged norbornene.13C NMR analyses show that the microstructures of the ethylene-norbornene copolymers with low norbornene incorporation are predominantly alternated and isolated norbornene units,while those with high norbornene incorporation are random polymers containing long norbornene sequences.

关键词： catalyst ethylene norbornene polymerization copolymerization

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Hemorrhagic activity and mechanism of FIIa,a fibrinolytic enzyme from Agkistrodon acutus venom

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Acta Pharmacologica Sinica 2004年第4期25卷 116-123页

作者： Qing-qing WANG2. Jia-shu CHEN, Xiu-xia LIANG3, Peng-xin QIU, Yi-wen WANG, Guang-mei YAN department of Pharmacology, zhongshan Medical College,{2., Guangzhou 510080, china Now in Beijing universityof Chinese medicine,Beijing 10002.,china. department of Pharmacology Zhongshan Medical College Sun Yat-Sen University

AIM: To study the local hemorrhagic activity of a fibrinolytic enzyme (FIIa) from Agkistrodon acutus venom and its mechanism. METHODS: The local hemorrhagic activity was determined by subcutaneous injection on the back of mouse. The effects of FIIa on factor X, prothrombin, gelatin, and collagen were visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Platelet aggregation assays were performed in rat platelet- rich plasma (PRP). Human umbilical vein endothelial cells (HUVEC) were cultured and passaged in complete M199 medium. Cell viability and nuclear morphology change were determined by fluorescein diacetate (FDA) staining and Hoechst 332.8 staining, respectively. RESULTS: The minimum hemorrhagic dose (MHD) of FIIa was 89 μg. In vitro, FIIa (0.2. g/L) degraded factor X, prothrombin, collagen, and gelatin, and dose-dependently (0.2., 0.50, 0.75, and 1.00 g/L) inhibited the platelet aggregation induced by ADP in rat PRP. When HUVEC in culture treated with FIIa, HUVEC showed detachment in a dose-dependent manner, but no apoptosis sign was observed. CONCLUSION: FIIahad local hemorrhagic activity, and the mechanism was related to the degradation of factor X, prothrombin, gelatin, and collagen, the inhibition of ADP-induced platelet aggregation, and inducement of HUVEC detachment.2.ow in Beijing universityof Chinese medicine,Beijing 10002.,{2..3Correspondence to Dr Xiu-xia LIANG.

关键词： Agkistrodon acutus venom fibrinolytic enzyme hemorrhagic protease endothelial cells

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Spontaneous chiral-symmetry breaking of lattice QCD with massless dynamical quarks

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Science china(Physics,Mechanics & Astronomy) 2007年第1期50卷 6-14页

作者： LUO XiangQian department of Physics,school of Physics and Engineering,{2. yat-sen university,Guangzhou 5102.5,china 1. department of Physics School of Physics and Engineering Sun Yat-Sen University Guangzhou 510275 China

One of the most challenging issues in QCD is the investigation of spontaneous chiral-symmetry breaking, which is characterized by the non-vanishing chiral condensate when the bare fermion mass is zero. In standard methods of the lattice gauge theory, one has to perform expensive simulations at multiple bare quark masses, and employ some modeled functions to extrapolate the data to the chiral limit. This paper applies the probability distribution function method to computing the chiral condensate in lattice QCD with massless dynamical quarks, without any ambiguous mass extrapolation. The results for staggered quarks indicate that this might be a promising and efficient method for investigating the spontaneous chiral-symmetry breaking in lattice QCD, which deserves further investigation.

关键词： lattice QCD spontaneous chiral-symmetry breaking dynamical quarks

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Purification and partial characterizations of coagulant protein Fla from Daboia russelli siamensis (Myanmar) venom

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Acta Pharmacologica Sinica 2007年第10期28卷 1580-1584页

作者： Huan-huan sun~(1,3),Qi CHEN~(2.3),Xi LIN~1,Jia-shu CHEN~(1,4),Peng-xin QIU~1,Guang-mei YAN~~1 department of Pharmacology,{2. Medical College,sun yat-sen university Guangzhou 510080,china ~2.Guangdong Institute forDrug Control,Guangzhou 510000,china department of Pharmacology Zhongshan Medical College Sun Yat-Sen University Guangzhou China Guangdong Institute for Drug Control Guangzhou China

Aim:To purify and characterize the coagulant protein FIa from Daboia russellisiamensis (Myanmar) ***:FIa was purified from Daboia russellisiamensis (Myanmar) venom by ion-exchange chromatography on CM-SephadexC-50,and gel filtration on Sephadex G-75 and a Superdex 75 *** hemo-static activity of FIa was determined by the method of Williams and *** chromogenic substrates were used respectively to determine the activa-tion of factor X and *** fibrinogen-clotting activity of FIa wasdetermined by the method of Gao et *** saline was used as a negativecontrol while factor Xa and thrombin were used as positive controls,***:FIa,a coagulant protein,was achieved by ion-exchange chromatogra-phy and gel filtration with a molecular weight of 34 479 and an isoelectric point *** was shown to have strong hemostatic *** hemostatic activity of0.5 mg FIa was equal to that of 1.562. u *** primarily activated factor X,however,had no influence on prothrombin,nor did it cleave or clot ***:FIa is a factor X-activating enzyme,which could activate factor X tofactor Xa,but has no effect on prothrombin and fibrinogen.

关键词： snake venoms Daboia russelli siamensis factor X blood coagulation

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Electrochemical Characterization of Surface-modified LiMn_2._4 Cathode Materials for Li-ion Batteries

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Journal of Wuhan university of Technology(Materials Science) 2006年第4期21卷 56-59页

作者：禹筱元 College of Science South China Agricultural University Guangzhou 510642ChinaSchool of Physics & Engineering Sun Yat-sen University Guangzhou 510275China

To improve the performance, the surface of 12.n2.4 was coated with very fine MgO , Al2.3 and ZnO by solgel method, respectively. The structure and morphology of the coated materials were investigated by X-ray diffraction （ XRD ）, X-ray photoelectron spectroscopy （ XPS ） and scanning electron microscopy （SEM）. The charge and discharge performance of uncoated and surfnce modified 12.n2.4 spinel at 2.℃ and 55 ℃ were tested, using a voltage window of 3.0-4.35 V and a current deasity of 0. 1 C rate. There is a slight decrease in the initial discharge capacity relative to that of uncoated UMn2.O4, bat the cycle ability of 12.12.n2.4 coated by metal-oxide has remarkably been improved. The EIS measuremeuts of uncoated and Al2.3 -coated 12.n2.4 were carried out by a model 2.3 A potentiostatl galvanistat controUed by a computer using M2.0 software, and using a freqnency response analyzer （ Zsimpwin ） combined with a potentiostate （ PAR 2.3）. Coaseqnently, the reason for the improved cycle properties is that the surface modification reduces the dissolution of Mn , which results from the suppression of the electrolyte decomposition, and suppresses the formation of passivation film that acts as an electronic insulating layer. In conclusion, the use of surface modification is an effective way to improve the electrochemical performance of 12.n2.4 cathode material for lithium batteries.

关键词： lithium-ion batteries surface modification metal-oxide coating: 12.n2.4 cathode

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An adaptive spatial clustering method for automatic brain MR image segmentation

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Progress in Natural Science:Materials International 2009年第10期19卷 1373-1382页

作者： Jingdan Zhang,Daoqing Dai Center for Computer Vision and department of Mathematics,sun {2.-sen(zhongshan) university,Guangzhou 5102.5,china [a]Center for Computer Vision and department of Mathematics Sun Yat-Sen (Zhongshan) University Guangzhou 510275 China

In this paper,an adaptive spatial clustering method is presented for automatic brain MR image segmentation,which is based on a competitive learning algorithm-self-organizing map(SOM).We use a pattern recognition approach in terms of feature generation and classifier ***,a multi-dimensional feature vector is constructed using local spatial ***,an adaptive spatial growing hierarchical SOM(ASGHSOM) is proposed as the classifier,which is an extension of SOM,fusing multi-scale segmentation with the competitive learning clustering algorithm to overcome the problem of overlapping grey-scale intensities on boundary ***,an adaptive spatial distance is integrated with ASGHSOM,in which local spatial information is considered in the clustering process to reduce the noise effiect and the classification *** proposed method is validated by extensive experiments using both simulated and real MR data with varying noise level,and is compared with the state-of-the-art algorithms.

关键词： Self-organizing map Growing hierarchical SOM Adaptive spatial distance Image segmentation MR images

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A provable secure fuzzy identity based signature scheme

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Science china(Information Sciences) 2012年第9期55卷 2139-2148页

作者： WANG ChangJi 1,2.1 school of Information Science and Technology,sun {2.-sen university,Guangzhou 5102.5,china 2.Guangdong Province Information Security Key Laboratory,sun {2.-sen university,Guangzhou 5102.5,china 1. school of Information Science and Technology Sun Yat-sen University Guangzhou 510275 China2. Guangdong Province Information Security Key Laboratory Sun Yat-sen University Guangzhou 510275 China

To use biometrics identities in an identity based encryption system,Sahai and Waters first introduced the notion of fuzzy identity based encryption(FIBE) in *** et *** it to digital signature and introduced the concept of fuzzy identity based signature(FIBS) in 2.08,and constructed an FIBS scheme based on Sahai and Waters's FIBE *** this paper,we further formalize the notion and security model of FIBS scheme and propose a new construction of FIBS scheme based on bilinear *** proposed scheme not only provides shorter public parameters,private key and signature,but also have useful structures which result in more efficient verification than that of Yang et al.'s FIBS *** proposed FIBS scheme is proved to be existentially unforgeable under a chosen message attack and selective fuzzy identity attack in the random oracle model under the discrete logarithm assumption.

关键词： identity based cryptosystem fuzzy identity based encryption fuzzy identity based signature

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