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Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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science china(life sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3,ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3 &ZHAO MaoLin 1 1 beijing Agro-Biotechnology Research Center,beijing Academy of Agriculture and Forestry science,beijing 100097,china 2 Department of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,china 3 college of life science,capital normal university,beijing 100037,china 4 college of life science,Zhongkai university of Agriculture and Technology,Guangzhou 510225,china 5 college of Grassland science,Gansu Agricultural university,Lanzhou 730070,china 6 Department of Biology,Henan Institute of science and Technology,Xinxiang 453003,china 1. beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. Department of Chemistry and Chemical Engineering Hunan Institute of Engineering Xiangtan 411104 China 3. college of life science Capital Normal University Beijing 100037 China 4. college of life science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. college of Grassland science Gansu Agricultural University Lanzhou 730070 China 6. Department of Biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 236000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 384-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

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New fossil elaterid (Coleoptera: Polyphaga: Elateridae) from Yixian Formation of western Liaoning, china

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Progress in Natural science:Materials International 2007年第10期17卷 1244-1249页

作者： Chang Huali, Ren Dong and Shih Chungkun(college of life science, capital normal university, beijing 100037, china) college of life science Capital Normal University Beijing 100037 China

A new species of the elaterid genus Cryptocoelus Dolin and Nel, 2002 from the Late Jurassic to Early Cretaceous Yixian Formation in the western Liaoning, china is described. Diagnosis of the genus is revised, and the systematic position of the genus is briefly discussed. Because the elaterids originated in the Early to Mid-Jurassic, this new material from the Late Jurassic-Early Cretaceous will enhance our understanding of the transition between ancient elaterids and extant ones, bridge the gap of cryptic relationships between the Mesozoic cupedids and elaterids, and expand our knowledge of their evolutionary history.

关键词： Elateridae new species Yixian Formation china.

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Two new Jurassic stoneflies (Insecta: Plecoptera) from Daohugou, Inner Mongolia, china

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Progress in Natural science:Materials International 2008年第8期18卷 1039-1042页

作者： Yushuang Liu, Dong Ren * college of life science, capital normal university, 105 Xisanhuanbeilu, Haidian District, beijing 100037, china college of life science Capital Normal University 105 Xisanhuanbeilu Haidian District Beijing 100037 China

Two new genera and two new species of family Taeniopterygidae, Mengitaenioptera multiramis gen. et sp. nov. and Noviramonemoura trinervis gen. et sp. nov. are described. These fossils were collected from Daohugou Village (the Middle Jurassic), Inner Mongolia, china. Hitherto, these fossil species from Daohugou are the oldest taeniopterygids. Both simple and complex venations simultaneously occurred in this group, which reveals that the early diversification of taeniopterids was well underway by the Middle Jurassic. Therefore, we prefer to adopt the Comstock’s opinion that the venation of taeniopterids was derived from the simple venation of the older group (such as Palaeonemouridae) at much ancient geological time ago. Some of the descendants carried this simple venation; others evolved into complex venation with many additional crossveins and branches.

关键词： Plecoptera Taeniopterygidae Daohugou Inner Mongolia Middle Jurassic

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New fossil caddisfly from Middle Jurassic of Daohugou,Inner Mongolia,china(Trichoptera:Philopotamidae)

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Progress in Natural science:Materials International 2009年第10期19卷 1427-1431页

作者： Meixia Wang,Yunyun Zhao,Dong Ren college of life science,capital normal university,beijing 100037,china [a]college of life science Capital Normal University Beijing 100037 China

A new genus and new species,Juraphilopotamus lubricus *** ***.,from the Middle Jurassic Jiulongshan Formation of Daohugou,Inner Mongolia,china,is described and *** may be the first record of the family Philopotamidae in china,extending the geographic distribution of this family.A detailed description and illustration of the specimen along with a brief review of the fossil Philopotamidae are given.A proposal on dispersionandmigration of Philopotamidae and problems of the paleoenvironment of the Daohugou beds are discussed.

关键词： Insecta Juraphilopotamus Philopotamidae Middle Jurassic Biogeography

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Cloning, sequencing and expression of the coat protein gene of Cocksfoot mottle virus

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Progress in Natural science:Materials International 2007年第2期17卷 222-225页

作者： DONG Zhi, GUO Xiaoyu, ZHANG Hailong and ZHANG Feiyun ( college of life science, capital normal university, beijing 100037, china college of Resources Environment and Tourism, capital normal university, beijing 100037, china beijing Key Laboratory for Resources Environment and Geography Information System, beijing 100037, china) [a]college of life science Capital Normal University Beijing China [b]college of Resources Environment and Tourism Capital Normal University Beijing China [c]beijing Key Laboratory for Resources Environment and Geography Information System Beijing China

The coat protein (CP) gene of Cocksfoot mottle virus (CfMV) was amplified by RT-PCR and inserted into expression vector pGEX-4T-1, and the resulting plasmid was designated as pGEXCfMV-JANCP. The fusion protein GST-CP was expressed in BL21 (DE3) pLysS after IPTG induction. The results of SDS-PAGE and Western blot analysis showed that the CfMV-CP gene was efficiently expressed in E. coli BL21 (DE3) pLysS through IPTG induction and the 56.0 kD protein was obtained.

关键词： Cocksfoot mottle virus sequence analysis prokaryotic expression.

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Cu(Ⅱ) Potentiation of Alzheimer Aβ1-40 Cytotoxicity and Transition on its Secondary Structure

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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 765-772页

作者： Xue-Ling DAI~1 Ya-Xuan SUN~2 Zhao-Feng JIANG~2* 1 college of life science,capital normal university,beijing 100037,china 2 college of Applied sciences and Humanities,beijing Union university,beijing 100083,china college of life science Capital Normal UniversityBeijing 100037 China college of Applied sciences and Humanities Beijing Union UniversityBeijing 100083 China

Mounting evidence has shown that dyshomeostasis of the redox-active biometals such as Cuand Fe can lead to oxidative stress,which plays a key role in the neuropathology of Alzheimer's disease（AD）.Here we demonstrate that with the formation of Cu（Ⅱ）·Aβ1-40 complexes,copper markedly potentiatesthe neurotoxicity exhibited by β-amyloid peptide （Aβ）.A greater amount of hydrogen peroxide was releasedwhen Cu（Ⅱ）·Aβ1-40 complexes was added to the xanthine oxidase/xanthine system detected by potassiumiodide *** bound to Aβ1-40 was observed by electron paramagnetic resonance（EPR） *** dichroism （CD） studies indicated that copper chelation could cause a structuraltransition of Aβ.The addition of copper to Aβ introduced an increase on β-sheet as well as α-helix,whichmay be responsible for the aggregation of Aβ.We hypothesized that Aβ aggregation induced by copper maybe responsible for local injury in *** interaction between Cu2+and Aβ also provides a possible mechanismfor the enrichment of metal ions in amyloid plaques in the AD brain.

关键词： Alzheimer’s disease Cu(Ⅱ)·Aβ1-40 complex neuron injury Aβ secondary structure

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New fossil ommatid (Coleoptera:Archostemata:Ommatidae) from Jehol Biota of western Liaoning, china

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Progress in Natural science:Materials International 2006年第6期16卷 639-643页

作者： REN Dong, TAN Jingjing and GE Siqin (college of life science, capital normal university, beijing 100037, china Institute of Zoology, Chinese Academy of sciences, beijing 100080, china) [a]college of life science Capital Normal University Beijing China [b]Institute of Zoology Chinese Academy of Sciences Beijing China

In this paper one new genus and one new species of fossil ommatid, Odontomma trachylaena gen. et sp. nov., is described and illustrated from the Late Jurassic or Early Cretaceous Yixian Formation of western Liaoning, china. Based on wide epipleural space of elytron with several rows of cells, the new taxa is attributed to tribe Brochocoleini of Ommatidae and compared with genus Bro-chocoleus. Due to the fact that most other Chinese Mesozoic ommatids are not complete, the complete preservation of the new specimens reported here enabled us to determine the characteristics of the body of these ancient ommatid. Moreover, based on the well-preserved individual of this new genus, we may suppose the affinities of the new species with extant and extinct groups.

关键词： Ommatidae Late Jurassic Early Cretaceous Liaoning Province.

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Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells

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Progress in Natural science:Materials International 2007年第2期17卷 156-162页

作者： CHEN Zhiling, YU Yi, LIU Lina and XIA Guixian ( college of life science, capital normal university, beijing 100037, china Institute of Microbiology, Chinese Academy of sciences, beijing 100080, china) [a]college of life science Capital Normal University Beijing China [b]Institute of Microbiology Chinese Academy of Sciences Beijing China

The fission yeast Schizosaccharomyces pombe was used to identify genes from tobacco BY-2 cells that may play roles in cell cycle regulation. A cDNA encoding a protein homologous to the yeast CDC48 was isolated and the gene was designated as NtCDC48 . The cDNA contains an open reading frame coding for a predicted protein of 808 amino acids which comprises of two typical ATPase modules (aa 245-374 and aa 518-646) . Overexpression of NtCDC48 in tobacco BY-2 cells led to an increase in the mitotic index as well as to the formation of diffused mitotic spindles. NtCDC48-GFP fusion proteins are distributed ubiquitously through Gl to M phases, yet their subcellular localization varied regularly along with the cell cycle progression. These results indicate that NtCDC48 may play an important role in the regulation of cell cycle in BY-2 cells.

关键词： tobacco BY-2 suspension cells NtCDC48 spindle cell cycle.

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Landscape Change and its effect on the environment of Daqing City

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Progress in Natural science:Materials International 2005年第7期15卷 641-649页

作者： ZANG Shuying, ZHANG Jing and JIA Li ( college of life and Environment science, Harbin normal university, Harbin 150080, china college of Resources Environment & Tourism, capital normal university, beijing 100037, china college of Economics and Management, Northeast Agricultural university, Harbin 150030, china) college of life and Environment science Harbin Normal University Harbin 150080 China. college of Resources Environment & Tourism Capital Normal University Beijing 100037 China. college of Economics and Management Northeast Agricultural University Harbin 150030 China

In this paper, we will study the temporal-spatial landscape change from 1978 to 2001 in Daqing City, based on the mul- ti spectral scanner (MSS) and thematic mapper (TM) images of remote sensing (RS) of 1978, 1988, 1992, 1996 and 2001. In order to explain the effects of the component change on the whole landscape, we make a statistic analysis about the transfer probability matrix of the component types of the landscape by using the ARCGIS platform through the overlay analysis of different landscape components. Based on the transfer probability matrix, we calculate the in-transfer ratio, the out-transfer ratio and the contributor ratio of the special transfer process of the main landscape components. The results illustrate that the cultivated-land, grassland, swamp, and alkaline land are the main contributors resulting in the landscape change: the out-transfer ratios of grassland and swamp are much higher than the in-transfer ones; the in-transfer ratio of alkaline land is much higher than the out-transfer one; the in-transfer ratio of the land-lot is always higher than its out-transfer with the highest rate per year, although there is a small difference among the in-transfers and the out-transfers per year. The main landscape changing processes are the salination of cultivated-land, salination or desertification of grassland, salination and degeneration of swamp. Results in this paper can provide scientific basis for investigating the landscape change mechanism of resources-based city.

关键词： landscape change environmental effect transfer probability resources-based city.

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A new fossil genus of Mesosciophilidae(Diptera,Nematocera) with two new species from the Middle Jurassic of Inner Mongolia,china

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Progress in Natural science:Materials International 2009年第12期19卷 1837-1841页

作者： Tingting Li,Dong Ren~* college of life science,capital normal university,beijing 100048,china [a]college of life science Capital Normal University Beijing 100048 China

A new genus within the family Mesosciophilidae,Jurasciophila ***.,with two new species,J,curvula *** *** *** *** ***.,are described and *** are established based on fossil specimens with bodies and complete *** of them were collected from the late Middle Jurassic Jiulongshan Formation of Daohugou in southeastern Inner Mongolia,*** with two known genera,Mesosciophilina(Kovalev,1985) and Mesosciophilopsis(Blagoderov,1994),the new genus has transitional characters. The distribution pattern of Mesosciophilidae is briefly discussed.

关键词： Mesosciophilidae Middle Jurassic Jiulongshan Formation

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