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Surface modification of hydrophobic NaYF_4:Yb,Er upconversion nanophosphors and their applications for immunoassay

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Science china Chemistry 2011年第8期54卷 1292-1297页

作者： LIU ChengHui, WANG Zhe, WANG XiaoKe & LI ZhengPing Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education college of Chemistry and Environmental Science, Hebei university, Baoding 071002, china Key Laboratory of Medicinal Chemistry and Molecular Diagnosis Ministry of Education College of Chemistry and Environmental Science Hebei University Baoding China

β-NaYFa：Yb,Er upconversion nanophosphor （UCNP） is known as one of the most efficient NIR-to-visible upconversion mate- dais, which shows great potential in bioanalytical chemistry and bioimaging. However, its applications are greatly limited due to its low water dispersibility and thus poor biocompatibility. In this paper, poly（acrylic acid）（PAA）-based ligand exchange strategies are carded out to modify oleic acid-capped hydrophobic β-NaYF4：Yb,Er UCNPs into hydrophilic ones. After efficient surface modification, the presence of free carboxylic acid groups on the surfaces of UCNPs results in high solubility in water, and also allows further conjugation with NH2-containing biomolecules. Facilitated by the covalent linkage between the -COOH groups on UCNPs surfaces and -NHz groups in antigen/antibody, a sensitive immunosensor is constructed by using PAA-functionalized β-NaYFa：Yb,Er UCNPs as biolabels. Through monitoring the upconversion fluorescence intensity or fluorescent imaging of the final immunocomplexes, high sensitivity is achieved for the proposed immunoassay and as low as 0.1 ng/mL goat anti-human immunoglobulin G （IgG） can be detected, which suggests that PAA-modified UCNPs may serve as an ideal candidate for use as bioanalysis and bioimaging probes.

关键词： surface modification upconversion nanophosphor ligand exchange immunoassay bioimaging

The First Case of Captive Breeding Fetus of Malayan Pangolin(Manis javanica)

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The First Case of Captive Breeding Fetus of Malayan Pangolin...

Large range step method for acoustic waveguide with two layer media

第七届全国野生动物生态与资源保护学术研讨会

作者： Li Yang~1,Fuhua Zhang~1,Shibao Wu~(1,*),Guangzhi Ma~1,Shenlong Chen~3,Xiaoqiang Yuan~3,Jinchu Hu~2 (1 college of Life Science,South china Normal university,Guangzhou,510631,china 2 college of Life Science,china West Normal university,Nanchong,637001,china 3 Dongguan Jiankang animal Farm Dongguan,523570,Cnina)

This article describes the first case of captive breeding fetus of Malayan pangolin(Manis javanica),female,120g,with the body total length 19.8cm,has *** dam died of illness,has been made into specimens with the fetus specimens stored in the Biological Herbarium of college of Life Science at South china Normal *** male parent still kept in the Research Base of South china Normal university for the Pangolins Artificial Breeding and *** is the first successful case reported of breeding fetus of Manis javanica in captivity in the world,which indicated that preliminary captive propagation method of the Manis javanica has been mastered.

关键词： Malayan pangolin Manis javanica fetus captive breeding

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Progress in Natural Science:Materials International 2002年第11期 22-27页

作者： ZHU Jianxin and LU Yayan(1. Department of Mathematics, zhejiang university, hangzhou 310027, china 2. Department of Mathematics, City university of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, china) Department of Mathematics Zhejiang University Hangzhou 310027 China Department of Mathematics City University of Hong Kong Tat Chee Avenue Kowloon Hong Kong China

A numerical method is developed for solving the two-dimensional Helmholtz equation in a two-layer region bounded by a flat top, a flat bottom and a curved interface. A better local orthogonal transformation is used to flatten the curved interface of the waveguide. The one-way re-formulation based on the Dirichlet-to-Neumann (DtN) map is then used to reduce the boundary value problem to an initial value problem. Numerical implemetation of the resulting operator Riccati equation uses a large range step method for discretizing the range variable and a truncated local eigenfunction expansion for approximating the operators. This method is particularly useful for solving long range wave propagation problems in slowly varying waveguides.

关键词： Helmholtz equation numerical solution marching method acoustic waveguide.

Reduction of truss topology optimization

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Journal of Shanghai university(English Edition) 2009年第6期13卷 489-496页

作者：孙艳白延琴周轶凯 college of sciences Shanghai University

A reduction of truss topology design problem formulated by semidefinite optimization （SDO） is considered. The finite groups and their representations are introduced to reduce the stiffness and mass matrices of truss... 详细信息

关键词： truss topology optimization reduction semidefinite optimization (SDO) group representation theory

Global Adaptive Control of A Class of Uncertain Nonlinear Systems Using Neural Networks

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Global Adaptive Control of A Class of Uncertain Nonlinear Sy...

Influence of supports on the oxidation of glucose on nano-gold catalysts

第二十七届中国控制会议

作者： Chen Pengnian1,Qin Huashu2,Sun Mingxuan3 1.Department of Mathematics,china Institute of Metrology,hangzhou 310018,P.R.china 2.Institute of Systems Science,The Chinese Academy of sciences,Beijing 100080,P.R.china 3.college of Information Engineering,zhejiang Univewrsity of Technology,hangzhou 310014,P.R.china

The paper considers the problem of global adaptive tracking for a class of uncertain nonlinear systems in which the uncertainty is impossible to be *** the help of the technique of unit partition in differential topology,a result on global approximation of function using neural networks is *** the result,a method of global adaptive neural network control for the uncertain nonlinear system is *** method ensures that the tracking error converges to an arbitrarily given small neighborhood of *** the tracked signal is constant,the tracking error converges to zero.

关键词： Nonlinear system Adaptive control Neural network

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Influence of supports on the oxidation of glucose on nano-go...

A Novel Structure of LAPS Array for Cell-Based Biosensor

2011 World Congress on Engineering and Technology(CET 2011)

作者： Yue Tang 1 Mingqiao Zhu 2 Lin Huang 3 Yangyi Shen 4 Yangyang Yang 5 Department of Chemical and Biochemical Engineering zhejiang university hangzhou china 310027

Several kinds of nano-gold catalysts such as Au/TiO 2 ,Au/γ-Al 2 O 3 ,Au/SiO 2 and Au/MCM-41 were prepared by deposition precipitation method,using chloroauric acid as *** effects of supports on the catalytic performance for glucose oxidation were investigated and the catalysts were characterized by Transmission Electron Microscope （TEM）,X-Ray Diffraction （XRD）,Inductively Coupled Plasma-Atomic Emission Spectrometry （ICP-AES） and BET *** better catalyst is Au（1.0 wt%）/TiO 2 and the glucose conversion of approximately 100% in 1.5h was obtained.

关键词： glucose oxidation sodium gluconate gold catalyst

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稀有金属材料与工程 2006年第S3期 51-54页

作者： Ying Xu~(1,2) Hua Cai~1 Qingjun Liu~1 Lifeng Qin~1 Lijiang Wang~1 Ping Wang~(1,2) (1.Biosensor National Special Laboratory,Key Laboratory of Biomedical Engineering of Education Ministry,Department of Biomedical Engineering,zhejiang university,hangzhou 310027,china) (2.State Key Lab of Transducer Technology,Chinese Academy of sciences,Shanghai 200050,china)

The light-addressable potentiometric sensor (LAPS) is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor (EIS) *** depositing biocompatible layers on the sensing surface for cell culture, it can be used to detect bioelectrical parameters of *** characteristic curve for photocurrent versus applied bias voltage to the system shows a current-voltage curve (Ⅰ-Ⅴcurve).This technique can be used to detect the action potential changes towards different drugs based on the bias voltage dependence of an optical current,and provides a dynamic system by scanning light beam at the very cell on the sensor *** LAPS overcomes the limitation of recording sites,but high spatial resolution and sensitivity are also *** paper discussed a novel structure of LAPS array for extracellular monitoring to decrease potential noise *** characteristics of active recording array areas and cell culture conditions are measured.

关键词： light-addressable potentiometric sensor array LAPS sensitivity cell-based biosensor

ON PARAMETRIC FACTORIZATION OF BI-ORTHOGONAL LAURENT POLYNOMIAL WAVELET FILTERS

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Approximation Theory and Its Applications 2002年第2期18卷 31-37页

作者： Hu Junquan Huang Daren Zhang Zeyin (zhejiang university, china) zhejiang university China

In this paper, we study the factorization of bi-orthogonal Laurent polynomial wavelet matrices with degree one into simple blocks. A conjecture about advanced factorization is given.

关键词： Haar BI POLYNOMIAL WAVELET FILTERS ON PARAMETRIC FACTORIZATION OF BI-ORTHOGONAL LAURENT

Screening of Bioflocculant-Producing Strain by Ion Implantation and Flocculating Characteristics of Bioflocculants

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Plasma Science and Technology 2008年第3期10卷 394-397页

作者：李培睿李宗伟李宗义秦广雍霍裕平 Key Laboratory of Ion Beam Bio-Engineering Zhengzhou University Zhengzhou 450052 China College of Life Sciences Henan Normal University Xinxiang 453002 China Key Laboratory of Ion Beam Bio-Engineering Zhengzhou University Zhengzhou 450052 China college of Life sciences Henan Normal University Xinxiang 453002 China

A bioflocculant-producing mutator strain, NIM-192, was screened out through nitrogen ion implanting into F J-7 strain. The results showed that NIM-192 had good genetic stability and high flocculating activity, and the flocculating rate increased by 34.26% than that of the original. Sucrose, complex nitrogen source contained yeast extract, urea and pH 7.0～ 9.0 were chosen as the best carbon source, nitrogen source and initial solution pH for bioflocculant production, respectively. The bioflocculant kept high and stable flocculating activity at alkalinous reaction mixture with a pH beyond 7.0, while the flocculating activity was remarkably reduced when the reaction pH was lower than 7.0. Addition of many cations could obviously increase the flocculating rate, among which Ca^2＋ demonstrated the best effect. The bioflocculant had very strong acid-base stability and *** flocculating rate kept over 86% when pH of the bioflocculant was in a range of 3.0 ～ 12.0, and the change of flocculating activity was not great when heated at 100℃ for 60 rain.

关键词： ion implantation bioflocculant mutation breeding flocculating characteristics