检索结果-南通市图书馆

利用豆粕、菜粕和棉粕替代饲料中鱼粉对苏氏圆腹(鱼芒)摄食、生长和饲料利用的影响

在线全文

学校读者我要写书评

暂无评论

水产学报 2009年第3期33卷 479-487页

作者：刘修英王岩王建华上海海洋大学水产与生命学院华北水利水电学院职业学院浙江大学动物科学学院郑州市水产研究所

通过8周网箱实验评价了利用豆粕、菜粕和棉粕替代苏氏圆腹(鱼芒)饲料中鱼粉的潜力。配制了7种等氮、等能饲料,其中对照饲料含45%鱼粉,在其余6种饲料中按等量蛋白替代原则分别添加31%或46%豆粕替代基础饲料中鱼粉的50%或75%,添加20%或40... 详细信息

通过8周网箱实验评价了利用豆粕、菜粕和棉粕替代苏氏圆腹(鱼芒)饲料中鱼粉的潜力。配制了7种等氮、等能饲料,其中对照饲料含45%鱼粉,在其余6种饲料中按等量蛋白替代原则分别添加31%或46%豆粕替代基础饲料中鱼粉的50%或75%,添加20%或40%菜粕替代基础饲料中鱼粉的25%或50%,添加19%或39%棉粕替代基础饲料中鱼粉的25%或50%。实验中所用苏氏圆腹鱼芒初始体重为11.3 g。实验结果表明:添加豆粕将饲料鱼粉含量从45%降低到23%,添加菜粕或棉粕将鱼粉含量降低到34%,对鱼成活率、摄食、鱼体增重、特定生长率(SGR)、饲料系数(FCR)、饲料蛋白储积率、脏体指数和红血细胞比积(Hct)未产生显著不良影响。添加豆粕将鱼粉含量降低到11%导致鱼摄食、鱼体增重和SGR下降,添加菜粕将鱼粉含量降低到23%导致FCR升高和鱼体能量储积率下降,添加棉粕将鱼粉含量降低到23%导致鱼体增重、SGR和Hct下降。上述结果显示可通过添加豆粕将苏氏圆腹鱼芒鱼种饲料鱼粉含量降低到23%,或通过添加菜粕和棉粕将饲料鱼粉含量降低到34%。

关键词：苏氏圆腹(鱼芒) 豆粕菜粕棉粕鱼粉

The role of mesodermal signals during liver organogenesis in zebrafish

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china(Life sciences) 2010年第4期53卷 455-461页

作者： NIU XuBo, SHI Hui & PENG JinRong college of animal sciences, zhejiang university, hangzhou 310029, china college of animal sciences Zhejiang University Hangzhou China

Three germ cell layers, the ectoderm, mesoderm and endoderm, are established during the gastrulation stage. All cell types in different organs and tissues are derived from these 3 germ cell layers at later stages. For example, skin epithelial cells and neuronal cells are derived from the ectoderm, while endothelial cells and muscle cells from the mesoderm and lung, and intestine epithelial cells from the endoderm. While in a normal situation different germ cells are destined to specific cell fates in different organs and tissues, each type of germ cells or its derivatives also produce extracellular signaling molecules to direct and facilitate the specification and differentiation of other germ cells during organogenesis. Liver is derived from the endoderm, but completion of liver organogenesis is regulated at different levels. While the pan-endoderm factors (e.g. FoxA and Gata families) and liver specific factors (e.g. Prox1 and Hhex) are essential intrinsic factors for endoderm cells to be differentiated into hepatoblasts, the role of signals produced by neighboring mesoderm cells for liver organogenesis is equally important. This review summarizes recent progress in studying the role of Bone morphogenetic proteins (Bmp), Fibroblast growth factors (Fgf), retinoic acid (RA) and Wingless and Int (Wnt), the 4 types of signaling molecules produced by the mesoderm cells, in liver organogenesis in zebrafish.

关键词： liver organogenesis zebrafish BMP FGF WNT

Accumulation of Pathogenesis-related Type-5 Like Proteins in Phytoplasma-infected Garland Chrysanthemum Chrysanthemum coronarium

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第11期36卷 773-779页

作者： Bo-Xiong ZHONG and Yan-Wei SHENDepartment of Bioresource Science, college of animal Science, zhejiang university, hangzhou 310029, china Department of Bioresource Science College of Animal Science Zhejiang UniversityHangzhou 310029 China

Soluble proteins extracted from leaves, apical shoots, axillary shoots, and stems of garland chrysanthemum plants infected by onion yellows phytoplasma were analyzed by two-dimensional gel electrophoresis. Computerized matching analysis revealed that at least six soluble proteins were accumulated specifically in phytoplasma-infected garland chrysanthemum. N-terminal amino acids sequences of these soluble

关键词： phytoplasma garland chrysanthemum Chrysanthemum coronarium pathogenesis-related proteins thaumatin osmotin

Highly Sensitive Fluorescent-labeled Probes and Glass Slide Hybridization for the Detection of Plant RNA Viruses and a Viroid

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2007年第5期39卷 326-334页

作者： Zhiyou DU~(1,2), Bo JIN~3, Wenhong LIU~4, Liang CHEN~1, and Jishuang CHEN~(2,1*) 1 college of Life sciences, zhejiang university, hangzhou 310029, china 2 Institute of Bioengineering, zhejiang Sci-Tech university, hangzhou 310018, china 3 college of Life sciences, zhejiang Chinese Medical university, hangzhou 310053, china 4 college of Bioengineering, zhejiang Chinese Medical university, hangzhou 310053, china college of Life sciences Zhejiang UniversityHangzhou 310029 China Institute of Bioengineering Zhejiang Sci-Tech UniversityHangzhou 310018 China college of Life sciences Zhejiang Chinese Medical UniversityHangzhou 310053 China college of Bioengineering Zhejiang Chinese Medical UniversityHangzhou 310053 China

In this study, a modified method of the conventional RNA dot-blot hybridization was established, by replacing32p labels with CY5 labels and replacing nylon membranes with positive-charged glass slides, for detecting plant RNA viruses and a viroid. The modified RNA dot-blot hybridization method was named glass slide hybridization. The optimum efficiency of RNA binding onto the surfaces of activated glass slide was achieved using aminosilane-coated glass slide as a solid matrix and 5×saline sodium citrate （SSC） as a spotting solution. Using a CY5-labeled DNA probe prepared through PCR amplification, the optimized glass slide hybridization could detect as little as 1.71 pg of tobacco mosaic virus （TMV） RNA. The sensitivity of the modified method was four times that of dot-blot hybridization on nylon membrane with a32P-labeled probe. The absence of false positive within the genus Potyvirus [potato virus A, potato virus Y （PVY） and zucchini yellow mosaic virus] showed that this method was highly specific. Furthermore, potato spindle tuber viroid （PSTVd） was also detected specifically. A test of 40 field potato samples showed that this method was equivalent to the conventional dot-blot hybridization for detecting PVY and PSTVd. To our knowledge, this is the first report of using dot-blot hybridization on glass slides with fluorescent-labeled probes for detecting plant RNA viruses and a viroid.

关键词： glass slide hybridization fluorescent labeling dot-blot hybridization virus viroid

Phosphorus Concentration and Forms in Surface and Subsurface Drainage Water from Wetland Rice Fields in the Shaoxing Plain

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Pedosphere 2003年第3期13卷 239-248页

作者： ZHANG MINGKUI, JIANG HONG and LIU XINGMEIDepartment of Resource sciences, college of Resource and Environmental sciences, zhejiang university, hangzhou 310029 (china) Department of Resource sciences College of Resource and Environmental Sciences Zhejiang University Hangzhou 310029

Phosphorus (P) is the limiting factor for eutrophication in most freshwater ecosystems. In china, Ptransported from intensively cultivated land has been reported as an important source of P in surface *** this study, we investigated P concentration and forms in surface and subsurface drainage from wetland ricefields in the Shaoxing plain, zhejiang Province, china. From selected rice fields, surface drainage sampleswere collected at rice-growing, non-growing and fertilization periods, and subsurface drainage samples atdrought and rewetting (irrigation or precipitation after 5～10 d drought period in the surface soils) and wet(drainage under long-term wet soil condition) periods. Water samples were characterized for their totalreactive P (TRP), dissolved reactive P (DRP) and particulate reactive P (PRP). Concentrations of the TRPand DRP in the surface drainage ranged from 0.08 to 1.50 and 0.06 to 1.27 mg L-1, respectively. The TRPand DRP were dependent on field operation activities, and decreased in the order of fertilization period ＞rice-growing period ＞ non-growing period. Phosphorus concentration of runoff receiving P fertilizer can bean environmental concern. The PRP concentration in the surface drainage, ranging from 0.01 to 0.57 mgL-1, accounted for 8%～78% of the TRP. Concentration of the TRP in the subsurface drainage was from0.026 to 0.090 mg L-1, consisting of 29%～90 % of the DRP and 10%～71% of the PRP. In the droughtand rewetting period, the PRP accounted for, on average, 63% of the TRP, much higher than in the wetperiod (23%), suggesting that there was transport of P in preferential flow during drainage events after ashort-term drought period in the surface soils. Therefore, P losses in particulate form may be importantin the subsurface drainage from rice fields when surface soils form cracks and favor rapid flow downwardthrough the soil profiles, suggesting the important role of water-dispersible colloid particles in mediating andco-transporting P in

关键词： drainage phosphorus wetland rice field

Expression and Identification of Mutated Osteoprotegerin in Culture Cells and Larvae of Silkworm

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第5期36卷 331-335页

作者： Hai-Long XIAO, Yong-Feng JIN, and Yao-Zhou ZHANG1* Institute of Biochemistry, college of Life sciences, zhejiang university, hangzhou 310029, china college of Life sciences, zhejiang university of Science & Technology, hangzhou 310018, china Institute of Biochemistry College of Life Sciences Zhejiang University Hangzhou 310029 China college of Life sciences Zhejiang University of Science & Technology Hangzhou 310018 China

The mutated osteoprotegerin (OPG-372) gene was inserted into the baculovirus transfer vector pBacPAK8, and the recombinant plasmid was co-transfected with linearized Bm-BacPAK6 virus DNA into BmN cells, then homologous recombination occurred inside the cells. The recombinant virus BmNPV-OPG- 372 was screened and identified by Southern blotting. The recombinant human OPG-372 was expressed in cultured cells and the larvae of silkworm by inoculation of recombinant virus. The expression products were run on the SDS-PAGE and their immunoreactivities were determined by Western blotting. It was found that a 42 kD recombinant protein was expressed in BmN cells and a 46 kD one in larvae respectively. The bioactivi- ties of the recombinant proteins were determined by hypocalcemic effect assay in the mice. The results showed that the recombinant proteins had a significant hypocalcemic effect on mice sera.

关键词： OPG-372 silkworm baculovirus expression system hypocalcemic effect

Occurrence of nitrification-denitrification and gaseous nitrogen loss process in flooded rice soil

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2007年第1期17卷 6-10页

作者： NI Wuzhong, LI Jianping and ZHU Zhaoliang (college of Environmental and Resource sciences, zhejiang university, hangzhou 310029, china college of Biosystems Engineering and Food Science, zhejiang university, hangzhou 310029, china Institute of Soil Science, Chinese Academy of sciences, Nanjing 210008, china) college of Environmental and Resource sciences Zhejiang University Hangzhou 310029 China college of Biosystems Engineering and Food Science Zhejiang University Hangzhou 310029 China Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 China

Flooding in wetland rice fields soon after transplanting results in displacement of soil air (including O2). Thus any dissolved O2 in the pore water is consumed out by microbial respiration in a short period. Supply of O2 to the flooded rice soil is by diffusion of O2 through the standing floodwater and consumption at the soil-water interface, and by exudation of O2 by rice roots and subsequent diffusion of O2 into the rhizosphere. The greater potential consumption of O2 compared to the renewal rate results in the development of distinct soil layers: oxidized soil layers under soil-water interface and in the rhizosphere, and reduced soil layers or reduced bulk soil. Nitrification in oxidized soils and denitrification in reduced soils have been known. Currently, denitrification in oxidized soils, even in standing floodwater, has also been identified. In this article, we present a modified nitrification and denitrification occurring mechanism in flooded rice soil.

关键词： denitrification flooded rice soil gaseous nitrogen loss nitrification.

Plant MITEs: Useful Tools for Plant Genetics and Genomics

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2003年第2期1卷 90-100页

作者： Ying FengAgriculture and Biotechnology college, zhejiang university, hangzhou 310029, china Agriculture and Biotechnology college Zhejiang University Hangzhou 310029 China. qzhxue@***

MTTEs (Miniature inverted-repeat transposabie elements) are reminiscence ot non-autonomous DNA (class Ⅱ) elements, which are distinguished from other transposable elements by their small size, short terminal inverted repeats (TIRs), high copy numbers, genie preference, and DNA sequence identity among family members. Although MITEs were first discovered in plants and still actively reshaping genomes, they have been isolated from a wide range of eukaryotic organisms. MITEs can be divided into Tourist-like, Stowaway-like, and pogo-like groups, according to similarities of their TIRs and TSDs (target site duplications). In despite of several models to explain the origin and amplification of MITEs, their mechanisms of transposition and accumulation in eukaryotic genomes remain poorly understood owing to insufficient experimental data. The unique properties of MITEs have been exploited as useful genetic tools for plant genome analysis. Utilization of MITEs as effective and informative genomic markers and potential application of MITEs in plants systematic, phylogenetic, and genetic studies are discussed.

关键词： miniature inverted-repeat transposable element MITE transposable element retrotransposon evolution plant

Comparison of Transformation Efficiency of piggyBac Transposon among Three Different Silkworm Bombyx mori Strains

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2007年第2期39卷 117-122页

作者： Boxiong ZHONG~1 Jianying LI~1 Jin'e CHEN~(1,2) Jian YE~1 and Songdong YU~1 ~1college of animal sciences,zhejiang university,hangzhou 310029,china ~2Institute of Sericultural Research,zhejiang Academy of Agricultural sciences,hangzhou 310021,china college of animal sciences Zhejiang University Hangzhou 310029 China Institute of Sericultural Research Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China

The transformation rate of three different strains of silkworm Bombyx mori was comparedafter the introduction of enhanced green fluorescence protein （EGFP）-encoding genes into the silkwormeggs by microinjection of a mixture of piggyBac vector and helper plasmid containing a *** there were no significant differences among the three strains in the percentages offertile moths in microinjected eggs （P=0.1258）,the percentages of G0transformed moths in fertile mothsand injected eggs were both significantly different （P=0.01368 and P=0.02398, respectively）.Thetransformation rate of the Nistari strain （Indian strain） was significantly higher than that of the other twostrains,Golden-yellow-cocoon （Vietnamese strain） and Jiaqiu （Chinese strain）,which had similar rate. Theseresults indicate that the transformation efficiency of the piggyBac-based system might vary with silkwormstrains with different genetic *** presence of endogenous piggyBac-like elements might bean important factor influencing the transformation efficiency of introduced piggyBac-derived vectors,andthe diverse amount and activation in different silkworm strains might account for the significant differences.

关键词： Bombyx mori piggyBac transposon transgenesis transformation efficiency