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Fusion Protein of Interleukin 4 and Diphtherial Toxin with High Cytotoxicity to Cancer Cells

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 437-442页

作者： Yu-Jian ZHANG, Xiao-BoHU, Su-Xia LI1, Li-Ping TIAN, Sheng-Li YANG, and Yi GONG* Research center of Biotechnology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200233, china state key laboratory of Bioreactor Engineering, East china University of Science and Technology, Shanghai 200237, china Research center of Biotechnology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200233 China state key laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai 200237 China

Receptor of human interleukin 4 (hIL4R) has been found to be present on many types of cancer, so it may be a good target for cancer therapy. Here, fusion toxin gene DT4H has been constructed by fusing DNA sequence encoding the first 389 amino acids of diphtherial toxin (DT), which can not bind its own receptor, to human interleukin 4 (hIL4) gene. In order to improve the affinity of fusion toxin for hIL4R, a circularly permuted form of hIL4 (cpIL4) was used. The fusion gene was expressed in Escherichia coli where the fusion toxin DT4H was highly expressed. Purified DT4H was very cytotoxic to cancer cell line U251 cells, and moderate cytotoxic to HepG2 and MCF-7 cells. SGC-7901 cells were insensitive to it. The cytotoxic action of DT4H was specific because it was blocked by excess hIL4. These results suggest that DT4H may be a useful agent in the treatment of certain malignancies.

关键词： fusion toxin diphtherial toxin cancer therapy interleukin 4 IL-4 receptor

EFFECT OF PARTICLE SIZE ON THE FILMING PROCESS OF POLYSTYRENE LATEX STUDIED BY AFM

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Chinese Journal of Polymer Science 2002年第6期20卷 509-515页

作者： Xiao-zhong Qu Yi Shi Liu-sheng Chen Xi-gao Jin state key laboratory of Polymer Physics & Chemistry, center for Molecular Science,Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080 china state key Lab. of Polymer Phy./Chem. Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China

The film formation process of micro-PS particles (diameter 742 nm) and nano-PS particles (diameter 29 nm) was studied by atomic force microscopy and differential scanning calorimetry. During a step heating process, the particles were annealed for 0.5 h at each selected temperature. It was found that the deformation and interdiffusion temperatures of the micro-PS particles are ca. 120-130degreesC and 140-150degreesC, that of the nano-PS particles are 90degreesC and 100-110degreesC respectively. The DSC traces of nano-PS particles showed that there was an exothermic peak near T-g after annealing for 0.5 h at the selected temperatures below 90degreesC; otherwise, the exothermic peak disappeared after annealing at 100degreesC or above. Compared with the micro-PS particles, the sintering process of nano-PS particles occurs at much lower temperature determined by the confined state of polymer chains with higher conformational energy in nano-particles, and completes in a much narrower temperature range driven mainly by the larger total surface energy.

关键词： polystyrene latex filming process particle size effect

The multi-factor recombination and processes superimposition model for hydrocarbon accumulation:application to the Silurian in the Tarim Basin

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Petroleum Science 2008年第1期5卷 13-19页

作者： Meng Qingyang Pang Xiongqi Gao Jianbo state key laboratory of Petroleum Resource and Prospecting China University of Petroleum Beijing 102249 China Basin and Reservoir Research center China University of Petroleum Beijing 102249 China Research center CNOOC Beijing 100027 China

The multi-factor recombination and processes superimposition model for hydrocarbon accumulation is put forward in view of the hydrocarbon geological characteristics of multiple episodes of structural evolution, multiple sets of source-reservoir-seal assemblage, multiple cycles of hydrocarbon accumulation and multiple episodes of readjustment and reconstruction in the complex superimposed basins in china. It is a system including theories and methods that can help to predict favorable exploration regions. According to this model, the basic discipline for hydrocarbon generation, evolution and distribution in the superimposed basins can be summarized in multi-factor recombination, processes superimposition, multiple stages of oil filling and latest stage preservation. With the Silurian of the Tarim basin as an example, based on the reconstruction of the evolution history of the four factors （paleo-anticline, source rock, regional cap rock and kinematic equilibrium belt） controlling hydrocarbon accumulation, this model was adopted to predict favorable hydrocarbon accumulation areas and favorable exploration regions following structural destruction in three stages of oil filling, to provide guidance for further exploration ofoil and gas in the Silurian of the Tarim basin.

关键词： Multi-factor recombination processes superimposition Tarim Basin Silurian favorable exploration region prediction

Inconspicuous anchoring effects generated by false information

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Progress in Natural Science:Materials International 2008年第11期18卷 1375-1382页

作者： Chen Qu~(a,b) Jun Wang~a Yuejia Luo~(a,c,*) a state key laboratory of Cognitive Neuroscience and Learning,Beijing Normal University,Beijing 100875,china b center for studies of Psychological Application,South china Normal University Guangzhou,510631,china c key laboratory of Mental Health,Institute of Psychology,Chinese Academy of Sciences,Beijing 100101,china state key laboratory of Cognitive Neuroscience and Learning Beijing Normal University Beijing 100875 China center for studies of Psychological Application South China Normal University Guangzhou 510631 China key laboratory of Mental Health Institute of Psychology Chinese Academy of Sciences Beijing 100101 China

The impact of false information on numerical judgments was examined on young normal subjects by an event-related potential(ERP) *** imitate the judgments in real world,we ensured the subjects acknowledged of the target *** behavioral results found that both uncertain information and false information assimilated the final estimates:higher after higher anchors and lower after lower anchors;and false information caused a weaker anchoring bias than uncertain *** results provided further electro- physiological evidence for the mechanism of *** the early phrase,it was an accessibility-dominated process in which two kinds of anchors elicited an N300 component related to the accessibility of anchors *** knowledge relevant to targets joined the process in the late phrase,which caused a larger amplitude of late positive component(LPC)for implausible lower anchors than that for plausible higher *** analysis showed that medial frontal gyrus,whose activity was suggested to signal the need of adjust- ment,was more reliable to explain the LPC elicited by implausible lower ***,we suggest that accessibility is facilitated when the external anchor is consistent with the world knowledge,and adjustment is initiated when the external anchor is inconsistent.

关键词： False anchors Anchoring effects Accessibility Adjustment ERP

LEAFY HEAD2,which encodes a putative RNA-binding protein,regulates shoot development of rice

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Cell Research 2006年第3期16卷 267-276页

作者： Guo Sheng Xiong~(1,3) Xing Ming Hu~2 Yong Qing Jiao~1 Yan Chun Yu~1 Cheng Cai Chu~1 Jia Yang Li~1 Qian Qian~2 Yong Hong Wang~11 state key laboratory of Plant Genomics and National center for Plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,china 2 state key laboratory of Rice Biology,china National Rice Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310006,china 3 Graduate University of Chinese Academy of Sciences,Beijing 100049,china state key laboratory of Plant Genomics and National center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China Graduate University of Chinese Academy of Sciences Beijing China state key laboratory of Rice Biology China National Rice Research Institute Chinese Academy of Agricultural Sciences Hangzhou China

During vegetative development,higher plants continuously form new leaves in regular spatial and temporal *** with abnormal leaf developmental patterns not only provide a great insight into understanding the regulatorymechanism of plant architecture,but also enrich the ways to its modification by which crop yield could be ***,we reported the characterization of the rice leafy-head2 (lhd2) mutant that exhibits shortened plastochron,dwarfism,reduced tiller number,and failure of phase transition from vegetative to reproductive *** and histologicalstudy revealed that the rapid emergence of leaves in lhd2 was resulted from the rapid initiation ofleafprimordia whereasthe reduced tiller number was a consequence of the suppression of the tiller bud *** molecular and geneticanalysis showed that LHD2 encodes a putative RNA binding protein with 67% similarity to maize *** ofgenome-scale expression profiles between wild-type and lhd2 plants suggested that LHD2 may regulate rice shoot devel-opment through KNOXand hormone-related *** similar phenotypes caused by LHD2 mutation and the conservedexpression pattern of LHD2 indicated a conserved mechanism in controlling the temporal leaf initiation in grass.

关键词： phyllotaxy plastochron LHD2 RNA-binding protein stem elongation plant architecture Oryza sativa L

Biogenic Isoprene Emission Mechanism from ^(13)CO_2 Exposure Experiments

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Chinese Chemical Letters 2002年第9期13卷 897-900页

作者： Min SHAO state Joint key laboratory of Environmental Simulation and Pollution Control, center for Environmental Sciences, Peking University, Beijing 100871 state Joint key laboratory of Environmental Simulation and Pollution Control Center for Environmental Sciences Peking University Beijing 100871 China

Biogenic isoprene emissions have been believed to be from only photosynthesis processes in plant. However nocturnal isoprene emission from pine is detected. And by feeding 13CO2 to plants, it is found that both photos... 详细信息

关键词： Emissions volatile organic compounds (VOCs) 13C labeling isoprene.

Hydrogen metabolic pathways of Rhodospirillum rubrum under artificial illumination

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Chinese Science Bulletin 2010年第1期55卷 32-37页

作者： ZHU RuiYan1,2,3 & LI JiLun1 1 College of Biological Sciences, state key laboratory for Agrobiotechnology, china Agricultural University, Beijing 100193, china 2 College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, china 3 Engineering Research center for Bioenergy of Hebei Province, Qinhuangdao 066004, china College of Biological Sciences State Key Laboratory for Agrobiotechnology China Agricultural University Beijing China College of Environmental and Chemical Engineering Yanshan University Qinhuangdao China Engineering Research center for Bioenergy of Hebei Province Qinhuangdao China

Multiple enzymes involved in hydrogen metabolism have been detected in photosynthetic bacterium Rhodospirillum rubrum un-der various growth conditions. To investigate hydrogen metabolic pathways and the contribution of each pathway to hydrogen photoproduction in R. rubrum under the artificial illumination condition, three mutants were constructed, including nifHanfDG double mutant lacking both Fe-nitrogenase and Mo-nitrogenase, anfDG mutant lacking Fe-nitrogenase and nifHhupL double mu-tant (uptake hydrogenase deficient mutant). Comparison of the hydrogen production of these 3 mutants with R. rubrum wild type and the uptake hydrogenase deficient mutant showed that there was a third pathway involved in hydrogen production besides Mo-nitrogenase and Fe-nitrogenase, which mainly contributed to hydrogen evolution. Only a small portion of hydrogen was gen-erated by the third pathway. The hydrogen produced by Mo-nitrogenase, Fe-nitrogenase and the third pathway was about 93.5%, 4.9% and 1.5%, respectively, while the hydrogen consumed by uptake hydrogenase was about 13.3%. The investigation of the formate-linked hydrogenase activity indicated that the third pathway for hydrogen production was not mediated by the for-mate-linked hydrogenase, but probably by some unknown enzyme.

关键词：氢代谢人工照明代谢途径红螺菌固氮酶脱氢酶突变体体吸收

Identification of Prorocentrum minimum and Takayama pulchella by fluorescence in situ hybridization through epifluorescence microscopy and flow cytometry

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Acta Oceanologica Sinica 2009年第2期28卷 103-114页

作者： HOU Jianjun LAI Hongyan HUANG Bangqin CHEN Jixin College of Fisheries Huazhong Agricultural University Wuhan 430070 China College of Life Sciences Hubei Normal University Huangshi 435002 China state key laboratory of Marine Environmental Science Environmental Science Research Center Xiamen University Xiamen 361005 China key laboratory of Freshwater Fish Germplasm Resources and Biotechnology Yangtse River Fisheries Research Institute Jinzhou 434000 China state key laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China

Partial rDNA sequences of Prorocentrum minimum and Takayama pulchella were amplified, cloned and sequenced, and these sequence data were deposited in the GenBank. Eight oligonucleotide probes （DNA probes） were designed based on the sequence analysis. The probes were employed to detect and identify P. minimum and T. pulchella in unialgal and mixed algal samples with a fluorescence in situ hybridization method using flow cytometry. Epifluorescence micrographs showed that these specific probes labeled with fluorescein isothiocyanate entered the algal cells and bound to target sequences, and the fluorescence signal resulting from whole-cell hybridization varied from probe to probe. These DNA probes and the hybridization protocol we developed were specific and effective for P. minimum and T. pulchella, without any specific binding to other algal species. The hybridization efficiency of different probes specific to P. minimum was in the order： PM18S02 PM28S02 〉 PM28S01 〉PM18S01, and that of the probes specific to T. pulcheUa was TP18S02 TP28S01 〉 TP28S02 〉TP18S01. The different hybridization efficiency of the DNA probes could also be shown in the fluorescent signals between the labeled and unlabeled cells demonstrated using flow cytometry. The DNA probes PM18S02, PM28S02, TP18S02 and TP28S01, and the protocol, were also useful for the detection of Mgae in natural samples.

关键词： oligonucleotide DNA probes Prorocentrum minimum Takayama pulcheUa fluorescence in situ hybridization flow cytometry

Research on Water Resources Conservation of Mountain River Based on the Concept of Region Partition

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Journal of Mountain Science 2011年第4期8卷 582-591页

作者： LIU Yubang TAO Qingyu LIANG Chuan WU Yong state key laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of Technology Chengdu 61o059 China state key laboratory of Hydraulics and Mountain River Engineering College of Water Resource & Hydropower Sichuan University Chengdu 61oo65 China Academic Journal center of Chengdu University of Technology Chengdu 61oo59 China

Presently concepts and methods related to water resources conservation of mountain rivers are seriously insufficient,and its level is far from being adaptable to the development of a harmonious *** mountain ecosystems play a key role in water resources conservation of mountain rivers,and the characteristics of mountain ecosystems and hydrologic features of mountain river follow strong temporal and spatial distribution,partition theory can be applied to the water resources conservation of mountain *** theory observes the following partition principles:regional relativity,spatial continuity,integralcounty,meeting management needs,hierarchical principle,and comparability *** it lays equal emphasis on both water resources conservation and environmental protection,on both water quality conservation and water quantity protection,on the combination of water features,water cycle and water *** the partition methods,index method and map superposition method will be applied in region *** example of region partition of water resources conservation in the upper reaches of the Yangtze River shows that the partition theory is practicable in water resources conservation of mountain rivers,and it provides a platform for future study in water resources conservation.

关键词： Water resources conservation Mountain river Region partition Mountain ecosystems