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cvhA Gene of Streptomyces hygroscopicus 10-22 Encodes a Negative Regulator for Mycelia Development

Acta biochimica et biophysica Sinica 2006年第4期38卷 271-280页

作者： Heng-An WANG~(1,2#) Lei QIN~(2#) Ping LU~(1*) Zhi-xuan PANG~2 Zi-xin DENG~(3*) Guo-Ping ZHAO~(2*) 1 biotechnology Institute,School of Agriculture and biology,shanghai jiaotong university,shanghai 201101,china.2 Laboratory of Molecular Microbiology,Institute of Plant Physiology and Ecology,shanghai Institute for biological sciences,Chinese Academy of sciences,shanghai 200032,china.3 bio-x life science research center,shanghai jiaotong university,shanghai 200030,china. biotechnologyInstitute SchoolofAgricultureandBiologyShanghaiJiaotongUniversityShanghai201101China LaboratoryofMolecularMicrobiology InstituteofPlantPhysiologyandEcologyShanghaiInstituteforBiologicalSciencesChinese

A five-gene cluster cvhABCDE was identified from Streptomyces hygroscopicus *** thefirst gene of this cluster,cvhA encoded a putative sensor histidine kinase with a predicted sensor domainconsisting of two trans-membrane segments at the N-terminus and a conserved HATPasecdomain at the *** C-terminus polypeptide of CvhA expressed in Escherichia coli was purified and shown to beautophosphorylated with [γ-32P]ATP in *** phosphoryl group was acid-labile and basic-stable,whichsupported histidine as the phosphorylation *** obvious difference of mycelia development was ob-served between the null mutant of cvhA generated by targeted gene replacement and the wild-type parentalstrain 10-22 grown on solid soya flour medium with 2%-8% glucose or sucrose,but the cvhA mutant couldform much more abundant aerial mycelia and spores than the wild-type strain on solid soya flour mediumsupplemented with 6%-8% mannitol,6%-8% sorbitol,4%-6% mannose,or 4%-6% *** pheno-type was complemented by the cloned wild-type cvhA gene,and no difference was observed for growthcurves of the cvhA mutant and the wild strain in liquid minimal medium with the tested sugars at a concen-tration of 4%,6% and 8%.We thus propose that CvhA is likely a sensor histidine kinase and negativelyregulates the morphological differentiation in a sugar-dependent manner in *** 10-22.

关键词： sensor histidine kinase aerial mycelia sugar metabolism

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Understanding SARS with Wolfram Approach

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Acta biochimica et biophysica Sinica 2004年第1期36卷 1-10页

作者： Da-Wei LI, Yu-xi PAN, Yun DUAN, Zhen-De HUNG, Ming-Qing xU, and Lin HE ( bio-x life science research center, shanghai Jiao Tong university, shanghai 200030, china.Institute for Nutritional science, the Chinese Academy of sciences, shanghai 200031, china.) bio-x life science research center Shanghai Jiao Tong University Shanghai 200030 China.

Stepping acquired immunodeficiency syndrome (AIDS), severe acute respiratory syndrome (SARS) as another type of disease has been threatening mankind since late last year. Many scientists worldwide are making great efforts to study the etiology of this disease with different approaches. 13 species of SARS virus have been sequenced. However, most people still largely rely on the traditional methods with some disadvantages. In this work, we used Wolfram approach to study the relationship among SARS viruses and between SARS viruses and other types of viruses, the effect of variations on the whole genome and the advantages in the analysis of SARS based on this novel approach. As a result, the similarities between SARS viruses and other coronavirusxes are not really higher than those between SARS viruses and non-coronaviruses.

关键词： genome sequence SARS visualization Wolfram approach

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Organic Solvents Mediate Self-assembly of GAV-9 Peptide on Mica Surface

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Acta biochimica et biophysica Sinica 2007年第4期39卷 285-289页

作者： Hai LI~(1,3) Feng ZHANG~1 Yi ZHANG~1* Jianhua HE~1 Jun HU~(1,2) 1 shanghai Institute of Applied Physics,Chinese Academy of sciences,shanghai 201800,china.2 bio-x life sciences research center.College of life science and biotechnology,shanghai jiaotong university,shanghai 200030,china.3 Graduate School of the Chinese Academy of sciences,Beijing 100049,china shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800 China Graduate School of the Chinese Academy of sciencesBeijing 100049 China bio-x life sciences research center College of Life Science and Biotechnology Shanghai Jiaotong UniversityShanghai 200030 China

Self-assembly of peptides into fibrils and other morphologies has attracted much attention inmany fields,especially in nanofabrication,pathology and *** this paper,self-assembly of GAV-9peptide in organic solvents,ethanol and acetone,was investigated using atomic force microscopy(AFM)and attenuated total reflectance-Fourier transform infrared spectroscopy(ATR-FTIR).The results indicatedthat GAV-9 self-assembled into various nanostructures in both solvents after deposited and evaporated *** with β-sheet conformation were observed in both solvents when the peptide concentrationwas higher than 280 μ***,ordered fibrils with β-sheet conformation were formed in ethanol,butnot in acetone,with a peptide concentration ranging from 7 μM to 28 μ*** attribute the formation ofvarious nanostructures to the different physicochemical properties of the polar organic solvents on the self-assembly of GAV-9 peptide.

关键词： self-assembly GAV-9 peptide organic solvents ATR-FTIR AFM

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Association of DRD2 polymorphisms and chlorpromazine-induced extrapyramidal syndrome in Chinese schizophrenic patients

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Acta Pharmacologica Sinica 2006年第8期27卷 966-970页

作者： Sheng-nan WU~(2,3,5,6) Rui GAO~(2,3,6) Qing-he xING~(2,3,6) Hua-fang LI~4 Yi-feng SHEN~4 Niu-fan GU~4 Guo-yin FENG~4 Lin HE~(2,3,7)2 bio-x life science research center,shanghai jiaotong university,shanghai 200030,china.3 Institute of Nutritional sciences,SIBS,Chinese Academy of sciences,shanghai 200031,china.4 shanghai Institute of Mental Health,shanghai 200030,china.5 shanghai Institute of Hypertension,Ruijin Hospital,Medical School of shanghai jiaotong university,shanghai 200025,china bio-x life science research center Shanghai Jiaotong University Shanghai 200030 China

Aim:Extrapyramidal syndrome (EPS) is most commonly affected by typical antip-sychotic drugs that have a high affinity with the D2 ***,manyresearch groups have reported on the positive relationship between the geneticvariations in the DRD2 gene and the therapeutic response in schizophrenia pa-tients as a result of the role of variations in the receptor in modulating *** this study,we evaluate the role DRD2 plays in chlorpromazine-induced EPS in schizophrenic ***:We identified seven SNP(singlenucleotide polymorphism) (-141Cins>del,TaqIB,TaqID,Ser311Cys,rs6275,rs6277and TaqIA) in the DRD2 gene in 146 schizophrenic inpatients (59 with EPS and 87without EPS according to the Simpson-Angus Scale) treated with chlorpromazineafter 8 *** alleles of all loci were determined by PCR (polymerase chainreaction).Results:Polymorphisms TaqlD,Ser311Cys and rs6277 were not poly-morphic in the population recruited in the present *** statistical signifi-cance was found in the allele distribution of-141Cins>del,TaqIB,rs6275 andTaqIA or in the estimated haplotypes (constituted by TaqIB,rs6275 and TaqIA) inlinkage disequilibrium between the two ***:Our results did notlend strong support to the view that the genetic variation of the DRD2 gene playsa major role in the individually variable adverse effect induced by chlorpromazine,at least in Chinese patients with *** results confirmed a previousstudy on the relationship between DRD2 and EPS in Caucasians.

关键词： dopamine receptors basal ganglia disease polymorphism chlorpromazine schizophrenia

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Answering a century old riddle: brachydactyly type Al

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Cell research 2004年第3期 179-187页

作者： Bo GAO, Lin HE Institute for Nutritional sciences, shanghai Institutes for biological sciences, Chinese Academy of sciences, shanghai 200031, china. Neuropsychiatric and Human Genetics Group, bio-x life science research center, shanghai jiaotong university, shanghai 200030, china. Department of biochemistry, Faculty of Medicine, The university of Hong Kong, Hong Kong, china.

In 1903, Farabee analyzed the heredity of the human digital malformation, brachydactyly, the first recorded disorder of the autosomal dominant Mendelian trait. In 1951, Bell classified this type of brachydactyly as type A1 (BDA1). Over 100 cases from different ethnic groups have so far been reported. However, the real breakthrough in identifying the cause of BDA1 has only taken place in the last few years with the progress of the mapping and identification of one of the genes responsible for this disorder, thus providing an answer for a century old riddle. In this article, we attempt to review the current state of knowledge on the genetic features of BDA1 with its century-old history and signalling pathway of IHH, and also discuss genotype-phenotype correlation not only of BDA1, but also of all types of brachydactyly.

关键词： Brachydactyly IHH GDF5 ROR2 BMPRIB.

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Accurate tracking control in LOM application

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Journal of university of science and Technology Beijing 2003年第1期10卷 74-78页

作者： Yadong Liu, Congxin Li, xingui Guo, and Decai WangNational Die & Mould CAD Engineering research center, shanghai jiaotong university, shanghai 200030, china National Die & Mould CAD Engineering research center Shanghai Jiaotong University Shanghai 200030 China

The fabrication of accurate prototype from CAD model directly in short time depends on the accurate tracking control and reference trajectory planning in (Laminated Object Manufacture) LOM application. An improvement on contour accuracy is acquired by the introduction of a tracking controller and a trajectory generation policy. A model of the x-Y positioning system of LOM machine is developed as the design basis of tracking controller. The ZPETC (Zero Phase Error Tracking Controller) is used to eliminate single axis following error, thus reduce the contour error. The simulation is developed on a Maltab model based on a retrofitted LOM machine and the satisfied result is acquired.

关键词： tracking control LOM ZPETC trajectory planning

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Predicting Protein Subcellular Location Using Digital Signal Processing

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Acta biochimica et biophysica Sinica 2005年第2期37卷 88-96页

作者： Yu-xi PAN Da-Wei LI Yun DUAN Zhi-Zhou ZHANG Ming-Qing xU Guo-Yin FENG Lin HE bio-x life science research center,shanghai jiaotong university,shanghai 200030,china.Institute for Nutritional sciences,shanghai Institutes for biological sciences.Chinese Academy of science,shanghai 200030.china.Neuropsvchiatric & Human Genetics Group,bio-x center shanghai jiaotong university,shanghai 200030.china bio-xlifescienceresearchcenter ShanghaiJiaotongUniversityShanghai200030China

The biological functions of a protein are closely related to its attributes in a *** the rapidaccumulation of newly found protein sequence data in databanks,it is highly desirable to develop an auto-mated method for predicting the subcellular location of *** establishment of such a predictor willexpedite the functional determination of newly found proteins and

关键词： sequence order effect digital signal processing digital Fourier transform (DFT) frequency domain covariance discriminant algorithm bioinformatics proteomics

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SHEsis,a powerful software platform for analyses of linkage disequilibrium,haplotype construction,and genetic association at polymorphism loci

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Cell research 2005年第2期15卷 97-98页

作者： Yong Yong SHI1,2, Lin HE2,3,*1bio-x life science research center, shanghai Jiao Tong university, shanghai, china.2Institute for Nutritional sciences, shanghai Institute of biological sciences, Chinese Academy of sciences, shanghai,china.3NHGG, bio-x center, shanghai Jiao Tong university, shanghai, china bio-xlifescienceresearchcenter ShanghaiJiaoTongUniversityShanghaiChina InstituteforNutritionalsciences ShanghaiInstituteofBiologicalSciencesChineseAcademyofSciencesShanghaiChina

In multiloci-based genetic association studies of complex diseases, a powerful and high efficient tool for analyses oflinkage disequilibrium (LD) between markers, haplotype distributions and many chi-square/p values with a large numberof samples has been sought for long. In order to achieve the goal of obtaining meaningful results directly from raw data,we developed a robust and user-friendly software platform with a series of tools for analysis in association study withhigh efficiency. The platform has been well evaluated by several sets of real data.

关键词： software linkage disequilibrium haplotype analysis genetic association study.

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Rietveld Quantitative Analysis of Carbides Precipitation in Normalized-tempered 2.25Cr-1Mo-0.25V Steel

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Journal of Wuhan university of Technology(Materials science) 2009年第6期24卷 922-926页

作者：张永涛张汉谦 School of Materials science and Engineering Shanghai Jiaotong University research Institute Baoshan Iron & Steel Co.Ltd.

The quantitative determination of the mass fractions of precipitates in steels is very difficult using traditional materials characterization techniques. The Rietveld full-pattern fitting algorithm was introduced to solve this problem. The precipitated multicomponents’ mass fraction of M3C, MC, M7C3 and M23C6 were evaluated precisely and relatively quickly. It is found evolution of carbides apparently occurs during tempering at high temperatures, and a two-step transformation mechanism is proposed for M7C3 during early tempering treatment. The method is an effective way on the investigation of precipitation kinetics, which may play a promising role in propertities’ enhancement and design of the heat-resistant steels.

关键词： 2.25Cr-1Mo-0.25V steel rietveld quantitative analysis precipitates

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Modeling Study of the Particle Pinning Effect on the Grain Boundary Movement

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Journal of Iron and Steel research International 2011年第S1期18卷 342-345页

作者： WANG xue-lun 1,FU Li-ming 2,WANG Wei 3,JIA Zhi-xin 1 (1.Rizhaosteel Technology center,Rizhao Iron and Steel Co Ltd.Rizhao 276806,china.2.School of Materials science and Engineering,shanghai jiaotong university,shanghai 200240 china.3 Department of Structural Steels,Baosteel research Institute,shanghai 201900,china. Rizhao Iron & Steel Co Ltd Rizhaosteel Technol Ctr Rizhao 276806 Peoples R China shanghai Jiao Tong Univ Sch Mat Sci & Engn Shanghai 200240 Peoples R China Baosteel Res Inst Dept Struct Steels Shanghai 201900 Peoples R China

In this work,based on the classical grain boundary (GB) formula and the principle of work-energy conversion,a new physically-based model has been developed to predict the particle pinning force concerning the interaction between second phase particles (SPPs) and the moving *** effect of particles pinning on the GB movement is *** modeling results can be applied to quantitatively determine the critical numbers of SPPs required for complete pining the grain growth,such as the critical SPPs number of the unit GB area,the critical number for single grain stagnation,the critical volume fraction of particles at a given particle *** predictions are in good agreement with the experimental results by Gladman.

关键词： the second-phase particle pinning force physical-based model

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