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Differentially Expression of Tual,a Tubulin-encoding Gene,during Flowering of Tea Plant Camellia sinensis (L.) *** Using cDNA Amplified Fragment Length Polymorphism Technique

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 653-662页

作者： Wan-Ping FANG~(1,2) Chang-Jun JIANG~1* Mei YU~1 Ai-Hua YE~1 Zhao-Xia WANG~(1,3) 1 Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of Agriculture,Anhui Agricultural University,hefei 2.0036,china 2 College Of Horticulture,Nanjing Agriculture University,Nanjing 210095,china 3 auhui institute of education,hefei 2.0036,china Key Laboratory of Tea Biochemistry and Biotechnology Ministry of Agriculture Anhui Agricultural UniversityHefei 230036 China College Of Horticulture Nanjing Agriculture UniversityNanjing 210095 China auhui institute of educationhefei 2.0036 China

The complementary DNA （cDNA） amplified fragment length polymorphism technique wasused to isolate transcript-derived fragments corresponding to genes involved in the flowering of tea *** sequence analysis of an approximately 300 bp differential fragment amplified by primer combi-nation E11M11revealed 80%-84% similarity to the corresponding part of an α-tubulin gene of other *** complete cDNA sequence of this α-tubulin was cloned by the rapid amplification of cDNA ends technique;its full length is 1537 bp and contains an open reading frame of 450 amino acid residues with two N-glycosylation sites and four protein kinase C phosphorylation *** deduced amino acid sequences didshow significant homology to the α-tubulin from other plants that has been reported to be a pollen-specificprotein and could be correlated with plant cytoplasm-nucleus-interacted male *** named this completecDNA *** nucleotide and amino acid sequence data of Tual have been recorded in the GenBanksequence database with the accession *** Tual gene was cloned into the pET-32aexpression system and expressed in Escherichia coli BL21trxB（DE3）.The molecular weight of expressedprotein was deduced to be approximately 49 *** blot analysis was used to identify the temporalexpression of Tual in tea *** studies of the effect of Tual protein on pollen tube growth indicatedthe Tual solution obviously promoted the growth of tea pollen tube.

关键词： cDNA-AFLP Camellia sinensis flower bud α-tubulin Western blot pollen

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Study on New Sensitive Method of Determination of Phosphorus by Solid Phase Spectrophotometry

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Chinese Chemical Letters 2000年第7期11卷 609-612页

作者： Yue Qin XEI Shao Min LIU Hong Wu TONG Ya DAI(Department of Chemistry, Anhui institute of education, hefei 230061 Department of Food Science, institute of Economics and Technology, University of Science and Technology of china, hefei 230052) Anhui Inst Educ Dept Chem Hefei 230061 Peoples R China Univ Sci & Technol china Inst Econ & Technol Dept Food Sci Hefei 230052 Peoples R China

The use of solid phase spectrophotometry for the determination of trace phosphorus in the system of phosphomolybdate-fructose is described. The adsorption of the system on anion-exchange resin is reported.

关键词： phosphorus solid phase spectrophotometry anion-exchanger resin adsorption

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吉林省2023年初中毕业学业水平考试评价与分析报告

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吉林省教育学院学报 2024年第4期 1-23页

作者： Junior High School Teaching Research and Training Department,Jilin Provincial institute of education 吉林省教育学院初中教研培训部

学科：数学成员：徐明国，贾长彬，梁凯毓吉林省初中毕业学业水平考试数学试卷具有时效性、稳定性和创新性。2023年初中毕业学业水平考试数学试卷持续贯彻新课程标准的理念，考查内容以第四学段数学学科核心知识为主，素材选取以人民教...

学科：数学成员：徐明国，贾长彬，梁凯毓吉林省初中毕业学业水平考试数学试卷具有时效性、稳定性和创新性。2023年初中毕业学业水平考试数学试卷持续贯彻新课程标准的理念，考查内容以第四学段数学学科核心知识为主，素材选取以人民教育出版社的教材为主，其他版本的教材为辅。试卷整体结构合理，试题以原创为主，难易适中，稳中有变。

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THE LIMITING CASE OF THIELE'S INTERPOLATING CONTINUED FRACTION EXPANSION

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Journal of Computational Mathematics 2001年第4期19卷 433-444页

作者： Jie-qing Tan (institute of Applied Mathematics, College of Science and CAD/CG Division College of Computer & Information hefei, University of Technology, hefei 230009, china) 中国科学技术大学安徽合肥 230009

By means of the determinantal formulae for inverse and reciprocal differences with coincident data points, the limiting case of Thiele's interpolating continued fraction expansion is studied in this paper and given numerical example shows that the limiting Thiele's continued fraction expansion can be determined once for all instead of carrying out computations for each step to obtain each convergent as done in [3].

关键词： Continued fraction Inverse difference Reciprocal difference Expansion.

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A Study of Mechanical Properties of the Iron-base Sintered Steel Adding Fine Cr-Fe Powder

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稀有金属材料与工程 2011年第S3期40卷 5-8页

作者： Chen Hua 1 , Liu Yongbing 2 , Li Yueying 2 , Cao Zhanyi 2 , Chai Zhilong 3 1 Key Laboratory of Advanced Structural Materials, Ministry of education, Changchun University of Technology, Changchun 130012, china 2 Key Laboratory of Automobile Materials, Ministry of education, Jilin University, Changchun 130022,china 3 Research institute of First Automobile Factory of china, Changchun 130011, china Changchun Univ Technol Sch Mat Sci & Engn Key Lab Adv Struct Mat Minist Educ Changchun 130012 Peoples R China Jilin Univ Key Lab Automobile Mat Minist Educ Changchun 130022 Peoples R China Res Inst First Automobile Factory china Changchun 130011 Peoples R China

This paper reports an investigation of the mechanical properties and the fracture mechanism of iron-based sintered alloy containing fine Cr-Fe powder particles 300 nm in size and in contents raging from 0.2-1.5 wt%. The iron-base alloy were sintered at 1120 ℃ for 40min. The results of the study revealed improvement in mechanical properties such as tensile strength, elongation and hardness of the sintered alloy. The fracture behaviors of the alloy were influenced significantly by the presence of Cr-Fe powder particles. The mechanical properties increased with increasing Cr content. Micrographs of fracture appeared dimple and quasi-cleavage.

关键词： sintered steel fine Cr-Fe powder mechanical properties

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Real-World Treatment Adherence to Smoking Cessation Medications and Association with Treatment Outcome—27 Centers,china,2017–2022

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china CDC weekly 2024年第22期6卷 522-529,I0001-I0003页

作者： Anqi Cheng Zhao Liu Xinmei Zhou Zheng Su Dan Xiao Chen Wang the china National Tobacco Cessation Cohort Study(CNTCCS)Study Group Department of Tobacco Control and Prevention of Respiratory Disease Center of Respiratory MedicineChina-Japan Friendship Hospital/WHO Collaborating Center for Tobacco Cessation and Respiratory Diseases Prevention/National Center for Respiratory Medicine/National Clinical Research Center for Respiratory Diseases/Institute of Respiratory MedicineChinese Academy of Medical SciencesBeijingChina Department of Pulmonary and Critical Care Medicine Center of Respiratory MedicineChina-Japan Friendship Hospital/WHO Collaborating Center for Tobacco Cessation and Respiratory Diseases Prevention/China National Center for Respiratory Medicine/China National Clinical Research Center for Respiratory DiseasesBeijingChina Chinese Academy of Medical Sciences and Peking Union Medical College BeijingChina 不详

What is already known about this topic?Medication non-adherence significantly impedes smoking cessation *** effective smoking cessation medications are accessible in china,realworld adherence and its correlation with treatment outcomes remain largely *** is added by this report?From December 2017 to January 2022,1,120 participants were recruited from 27 centers in all seven geographical regions of *** revealed that only 38.2%of smokers adhered to the prescribed 12-week smoking cessation treatment and those with a higher education level were more likely to be *** addition,smokers with good adherence were more likely to successfully quit *** are the implications for public health practice?The data obtained could enhance our understanding of smoking cessation practices in *** adherence is a crucial strategy to enhance the effectiveness of smoking cessation treatments and management provided to smokers.

关键词： china smoke likely

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Effects of Citral on Aspergillus flavus Spores by Quasi-elastic Light Scattering and Multiplex Microanalysis Techniques

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 277-283页

作者： Man LUO1,2, Li-Ke JIANG2, Yao-Xiong HUANG3, Ming XIAO4, Bo LI4, and Guo-Lin ZOU1* 1College of Life Sciences, Wuhan University, Wuhan 430072, china 2College of Life Sciences, Anhui Agricultural University, hefei 2.0036, china 3 institute of Biomedical Engineering, Ji’nan University, Guangzhou 510632, china 4Ministry of education Key Laboratory for Biodiversity Science and Ecological Engineering, the institute of Biodiversity Science, Fudan University, Shanghai 200433, china College of Life Sciences Wuhan UniversityWuhan 430072 China College of Life Sciences Anhui Agricultural UniversityHefei 230036 China institute of Biomedical Engineering Ji'nan UniversityGuangzhou 510632 China Ministry of education Key Laboratory for Biodiversity Science and Ecological Engineering the Institute of Biodiversity Science Fudan UniversityShanghai 200433 China

Citral refined from Litsea cubeba oil has been found to have a strong influence on fungi, especially Aspergillus flavus. Multiplex microanalysis and quasi-elastic light scattering techniques were applied to study the effects of citral on Aspergillus flavus spores from the levels of membrane, organelle and intracellular macromolecule. It was found that citral injured the wall and the membrane of A. flavus spore, resulting in decrease of its elasticity. After entering the cell, citral not only influenced the genetic expression of mitochondrion reduplication and its morphology, but also changed the aggregation of protein-like macromolecules. As a result, cells, organelles and macromolecules lost their normal structures and functions, eventually leading to the loss of germination ability of A. flavus spores. Since Litsea cubeba oil as food additive and antifungal agent is safe and less poisonous, it is important to elucidate the inhibitory mechanisms of Litsea cubeba oil on the germination ability of A. flavus spore.

关键词： citral Aspergillus flavus spore multiplex microanalysis techniques quasi-elastic light scattering technique

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Simulation of Tensile Behavior of Nickel Foam

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稀有金属材料与工程 2011年第S3期40卷 224-227页

作者： Zhao Mingjuan 1, Zhang Xiaolan 1, Zhao Longzhi 1,3, Zhang Jinsong 2 1 Key Laboratory of Ministry of education for Conveyance and Equipment, East china Jiaotong University, Nanchang 330013, china 2 institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, china 3 Key Laboratory of Superlight Materials & Surface Technology, Ministry of education, Harbin Engineering University, Harbin 150001, china E china Jiaotong Univ Key Lab Minist Educ Conveyance & Equipment Nanchang 330013 Peoples R China Harbin Engn Univ Minist Educ Key Lab Supertight Mat & Surface Technol Harbin 150001 Peoples R China Chinese Acad Sci Inst Met Res Shenyang 110016 Peoples R China

Nickel foam is widely used as the electrode, the catalyst carrier, sound absorption material and other application fields. The tensile behavior of the open cell nickel foam which is one of the most important parameters in the design process was investigated with the finite element method based on ANSYS software in this paper. The effects of strain rates and temperature on the yield strength of the nickel foam were obtained. The results show that the yield strength exhibit a nonlinear reduction with the increases of the temperature for the elastic bending of the struts of the reinforcement grew quickly depending on the temperature. However, the yield strength increases slowly with the improvement of the strain rate because the strain rate neither change the internal structure and properties such as the porosity and the aperture, nor alter the external circumstance in order to change the internal properties such as the temperature. The results of the simulation accord with those of the experiments, which is very important to understand the tensile behavior of the foam materials.

关键词： nickel foam ANSYS yield strength temperature strain rate

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Optimal reactions for the synthesis of superheavy nucleus ^(270)Hs

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Science china(Physics,Mechanics & Astronomy) 2006年第6期49卷 641-648页

作者： LIU Zuhua1,3 & BAO Jingdong1,2 1. Key Laboratory of Beam Technology and Material Modification of Ministry of education, Beijing Normal University, Beijing 100875, china 2. Physics Department, Beijing Normal University, Beijing 100875, china 3. china institute of Atomic Energy, Beijing 102413, china Key Laboratory of Beam Technology Material Modification of Ministry of education Beijing Normal University Beijing 100875 China Physics Department Beijing Normal University Beijing 100875 China china institute of Atomic Energy Beijing 102413 China

The superheavy nucleus 270Hs is expected to be a “double-magic” deformed nucleus. We have calculated its cross sections of evaporation residue for the reactions 248Cm(26Mg,4n)270Hs, 244Pu(30Si,4n)270Hs, 238U(36S,4n)270Hs and 226Ra(48Ca,4n)270Hs using a two-parameter Smoluchowski equation. It is found from our results that 226Ra(48Ca,4n)270Hs and 238U(36S,4n)270Hs are two optimal reactions for the synthesis of the superheavy nucleus 270Hs due to their large negative Q-values.

关键词： diffused fusion barrier,Smoluchowski equation,superheavy nuclei.

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A possible configuration of H_5^+ cluster

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Chinese Science Bulletin 2002年第14期47卷 1170-1171页

作者： ZHOU QinianDepartment of Computer education, Zhejiang institute of Science and Technology, Hangzhou 310033, china Department of Computer education Zhejiang Institute of Science and Technology Hangzhou 310033 China

On the basis of our previous work, a configuration of H5+ has been presented that is a square center structure, and the energy for the structure of H5+ has been obtained by IACQM. The result showed that there is an extreme energy Ee: -2.45981 [2 Ry] at R = 2.50a0. It indicates that the square center structure of H5+ system has the certain stability, which is a possible configuration of H5+.

关键词： H5+ IACQM channel energy configuration.

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