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Monitoring early responses to irradiation with dual-tracer micro-PET in dual-tumor bearing mice

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World Journal of Gastroenterology 2010年第43期16卷 5416-5423页

作者： Hui Wang, Jia-He Tian, Bai-Xuan Xu, Zhi-Wei Guan, ChangBin Liu, Rui-Min Wang, Ying-Mao Chen, Jin-Ming Zhang, Nuclear Medicine Department, The general hospital of the Chinese People’s Liberation Army and military Medical Postgraduate College, beijing 100853, china Bo Liu, Department of Hepatobiliary Surgery, The general hospital of the Chinese People’s Liberation Army and military Medical Postgraduate College, beijing 100853, china Bao-Lin Qu, Department of Radiation Oncology, The general hospital of the Chinese People’s Liberation Army and military Medical Postgraduate College, beijing 100853, china Nuclear Medicine Department The General Hospital of the Chinese People's Liberation Army and Military Medical Postgraduate College 28 Fuxing Road Haidian District Beijing 100853 China.

AIM: To monitor the early responses to irradiation in primary and metastatic colorectal cancer (CRC) with 18 Ffluorothymidine ( 18 F-FLT) and 18 F-fluorodeoxyglucose ( 18 F-FDG) small-animal position emission tomography (micro-PET). METHODS: The primary and metastatic CRC cell lines, SW480 and SW620, were irradiated with 5, 10 and 20, l l l , Gy. After 24 h, the cell cycle phases were analyzed. A dual-tumor-bearing mouse model of primary and metastatic cancer was established by injecting SW480 and SW620 cells into mice. micro-PET with 18 F-FLT and 18 F-FDG was performed before and 24 h after irradiation with 5, 10 and 20 Gy. The region of interest (ROI) was drawn over the tumor and background to calculate the ratio of tumor to non-tumor (T/NT) in tissues. Immunohistochemical assay and Western blotting were used to examine the levels of integrin β3, Ki-67, vascular endothelial growth factor receptor 2 (VEGFR2) and heat shock protein 27 (HSP27). RESULTS: The proportion of SW480 and SW620 cells in the G 2 -M phase was decreased with an increasing radiation dose. The proportion of SW480 cells in the G 0 -G 1 phase was increased from 48.33% ± 4.55% to 87.09% ± 7.43% (P 3.57% ± 2.65% to 66.59% ± 7.37% (P = 0.021). In micro-PET study, with increasing dose of radiation, 18 F-FLT uptake was significantly reduced from 3.65 ± 0.51 to 2.87 ± 0.47 (P = 0.008) in SW480 tumors and from 2.22 ± 0.42 to 1.76 ± 0.45 (P = 0.026) in SW620 tumors. 18 F-FDG uptake in SW480 and SW620 tumors was reduced but not significantly (F = 0.582, P = 0.633 vs F = 0.273, P = 0.845). Dose of radiation was negatively correlated with 18 F-FLT uptake in both SW480 and SW620 tumors (r = -0.727, P = 0.004; and r = -0.664, P = 0.009). No significant correlation was found between 18 F-FDG uptake and radiation dose in SW480 or SW620 tumors. HSP27 and integrin β3 expression was higher in SW480 than in SW620 tumors. The T/NT ratio for 18 F-FLT up

关键词： 18 F-fluorothymidine 18 F-fluorodeoxyglucose Irradiation Positron emission tomography Colorectal cancer

The influence of urine volume and fibrinogen in nephrotic syndrome therapy with anticoagnlant therapy and nitrogen mustard

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The influence of urine volume and fibrinogen in nephrotic sy...

APPLICATION OF PUNCTURING THE POINTS OPPOSITE TO THE DISEASED SIDE IN THE TREATMENT OF TRAINING INJURY IN TROOPS——A Report on 2560 Cases

第三届国际中西医结合肾病学术会议

作者： YANG Tao CAO Mingliang JIANG DexunDept. of Nephrology general hospital of beijing military region beijing (100700)

＜正＞Objective: To evaluate the influence of urine volume and fibrinogen in nephrotic syndrom therapy with antico-agnlant therapy and Nitrogen mustard. Methods: Record the change of urine volume and fibrinogen after therapy, and contrast thesechanges. Results: The urine volume changes in the therapies of Uroknase with nitrogen mustard and Heparin are obvious（P ＜ 0.05 ）. The Nitrogen mustard and Low molecular can cut down the fibrinogen obviously（P＜ 0. 01 ）. Conclusions: The therapy methodof using Urokinase with nitrogen mustard is superior in nephrotic syndrome patient.

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World Journal of Acupuncture-Moxibustion 1994年第4期4卷 3-4,8页

作者： Wang WenyuanNo. 292 hospital of beijing military region, beijing 100026,china

Puncturing points opposite to the diseased side was used to treat injuries in 2560soldiers during their *** instant effective rate and curative rate of the therapy was up to 96.25％and 38.44％respectively.

关键词： Puncturing points opposite to the diseased side military training injury

血清乙酰肝素酶在糖尿病并发急性冠脉综合征中的变化及临床意义

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中国实用内科杂志 2009年第10期29卷 933-934页

作者：段景琪吕肖锋北京军区总医院内分泌科北京10070 第三军医大学

目的探讨血清乙酰肝素酶(HPA)在糖尿病并发急性冠脉综合征(ACS)中的变化及临床意义。方法选取2006年7月至2008年10月北京军区总医院内分泌科28名健康志愿者,27例糖尿病(DM组)患者,52例ACS患者、46例糖尿病并发急性冠脉综合征组(DM+ACS组... 详细信息

目的探讨血清乙酰肝素酶(HPA)在糖尿病并发急性冠脉综合征(ACS)中的变化及临床意义。方法选取2006年7月至2008年10月北京军区总医院内分泌科28名健康志愿者,27例糖尿病(DM组)患者,52例ACS患者、46例糖尿病并发急性冠脉综合征组(DM+ACS组)进行研究。酶联免疫吸附法(ELISA)检测各组血清C反应蛋白(CRP)、基质金属蛋白酶9、HPA。检测各指标受试者工作特性曲线(ROC),判断并比较血清CRP、MMP-9和HPA检测临界值及检测价值。结果DM+ACS组血清HPA显著高于其他3组(P3和0.967,组间比较差异有统计学意义(P<0.01)。结论血清HPA变化与糖尿病并发急性冠脉综合征发生发展有关,HPA作为预测该类患者动脉粥样斑块不稳定或破裂的血清学指标优于CRP和MMP-9。

关键词：糖尿病急性冠状动脉综合征乙酰肝素酶基质金属蛋白酶9 C-反应蛋白

N-(β-Carboxypropionyl)luminol as a new chemiluminescence label in immunoassay

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Chinese Journal of Chemistry 1997年第2期15卷 123-129页

作者： ZHUANG,Hui-Sheng WANG,Qiong-E ZHANG,FanDepartment of Chemistry,Fuzhou University,Fuzhou,Fujian 350002,china.HUANG,Qiao-Jia TANG,Yu-ChaiFuzhou general hospital,Nanjing military region,Fuzhou,Pujian 350002,china Department of Chemistry Fuzhou University Fuzhou Fujian 350002 China Fuzhou general hospital Nanjing Military Region Fuzhou Fujian 350002 China

A new chemilurninescence label N-（β-carboxypropionyl）luminol （CPL） was used to label sheep anti-human IgG （SaHIgG）.The labeled antibody was stable and could be detected at least down to 10-170-16 *** molar incorporation ratio was estimated to be 0.26 mol of CPL per mol of *** were no apparent changes in the immunoreactivity of the labeled SaHIgG and in the quantum efficiency of the CPL after labeling.

关键词： Chemiluminescence immunoassay N-(β-carbaxypropionyl)luminol sheep anti-human IgG

Expression of p57^(kip2), Rb protein and PCNA and their relationships with clinicopathology in human pancreatic cancer

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World Journal of Gastroenterology 2003年第2期9卷 377-380页

作者： Hui Yue Yan-Li Na Xin-Li Feng Shu-Ren Ma Fu-Lin Song Bo Yang Department of Gastroenterology and Pathology, general hospital of Shenyang military region, Shenyang 110016, Liaoning Province, china Department of Gastroenterology General Hospital of Shenyang Military Region Shenyang 110016 Liaoning Province China. yh12070430@***

AIM: To investigate the effects of inhibiting factor of cellcycle regulation p57kip2, retinoblastinoma protein (Rb protein)and proliferating cell nuclear antigen (PCNA) in the genesisand progression of human pancreatic ***: The expression of p57kip2, Rb protein and PCNAin tumor tissues and adjacent tissues of 32 patients withpancreatic cancer was detected with SP ***: p57kip2 protein positive-expression rate in tumortissues of pancreatic cancer was 46.9 %, which was lowerthan that in adjacent pancreatic tissues (75.0 %) (x2=5.317,P＜0.05), p57kip2 protein positive-expression correlatedsignificantly with tumor cell differentiation (well-differentiationversus moderate or low-differentiation, P＜0.05) but did notcorrelate significantly with lymph node metastasis (lymph nodemetastasis versus non-lymph node metastasis, P＞0.05); Rbgene protein positive-expression rate in tumor tissues was50.0 %, which was also lower than that in adjacent pancreatictissues (78.1%) (x2=5.497, P＜0.05); PCNA positive-expression rate was 71.9 %, being higher than that inadjacent pancreatic tissues (43.8 %) (x2=5.189, P＜0.05),PCNA positive-expression also correlated significantly withtumor cell differentiation and lymph node metastasis (well-differentiation versus moderate or low- differentiation, lymphnode metastasis versus non-lymph node metastasis, P＜0.05).Rb protein positive-expression rate in the tumor tissues ofp57kip2 protein positive-expression group was 53.3 %; andRb protein positive-expression rate in the tumor tissues ofp57kip2 protein negative-expression group was 47.1%. Therewas no significant relationship between the two groups(r=0.16507, P＞0.05).CONCLUSION: The decreased expression of p57kip2, Rbprotein or over-expression of PCNA protein might contributeto the genesis or progression of pancreatic cancer, p57kip2,Rb protein and PCNA may play an important role in genesisand progression of pancreatic cancer.

关键词：胰腺癌临床病理学 p57^kip2 Rb蛋白增殖细胞核抗原

Identification of two novel missense WFS1 mutations,H696Y and R703H,in patients with non-syndromic low-frequency sensorineural hearing loss

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Journal of Genetics and Genomics 2011年第2期38卷 71-76页

作者： Yi Sun Jing Cheng Yanping Lu Jianzhong Li Yu Lu Zhanguo Jin Pu Dai Rongguang Wang Huijun Yuan Institute of Otolaryngology Chinese PLA General Hospital Beijing 100853 China Department of Otolaryngology Chinese PLA Wuhan General Hospital of Guangzhou Military Command Wuhan 430070 China Department of Obstetrics and Gynecology Chinese PLA General Hospital Beijing 100853 China Fuzhou general hospital of Nanjing military Command PLA Fuzhou 350025 China

Non-syndromic low-frequency sensorineural hearing loss （LFSNHL） is an unusual type of hearing loss in which frequencies ≤2000 Hz predominantly are affected. To date, different mutations in two genes, DIAPHI and WFSI, have been found to be associated with LFSNHL. Here, we report a five-generation Chinese family with postlingual and progressive LFSNHL. We mapped the disease locus to a 2.5 Mb region on chromosome 4p16 between markers SNP_A-2167174 and D4S431, overlapping with the DFNA6/14/38 locus. Sequencing of candidate gene revealed a heterozygous c.2086C〉T substitution in exon 8 of WFS1, leading to p.H696Y substitution at the C-terminus of Wolframin （WFS 1）. In addition, we performed mutational screening of WFS1 in 37 sporadic patients, 7--50 years of age, with LFSNHL. We detected a heterozygous c.2108G〉A substitution in exon 8 of WFSI, leading to p.R703H substitution in a patient. The H696 and R703 in WFS1 are highly conserved across species, including human, orangutan, rat, mouse, and frog （Xenopus）, Sequence analysis demonstrated the absence of c.2086C〉T or c.210gG〉A substitutions in the WFS1 genes among 200 unrelated control subjects of Chinese background, supporting the hypothesis that they represent causative mutations, and not rare polymorphisms. Our data provide additional molecular and clinical information for establishing a better genotype-phenotype correlation for LFSNHL.

关键词： DFNA6/14/38 WFS1 Mutation Low-frequency hearing loss