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potential land for plantation of Jatropha curcas as feedstocks for biodiesel in china

science china Earth sciences 2010年第1期53卷 120-127页

作者： WU WeiGuang 1,2,3,HUANG JiKun 1,3 & DENG XiangZheng 1,3 1 institute of Geographic sciences and Natural resources research,chinese academy of sciences,Beijing 100101,china 2 Department of Economics and Management,Zhejiang Forestry university,Linan 311300,china 3 Center for chinese agricultural policy,chinese academy of sciences,Beijing 100101,china 1. institute of Geographic sciences and Natural resources research Chinese Academy of Sciences Beijing 100101 China2. Department of Economics and Management Zhejiang Forestry University Linan 311300 China3. Center for Chinese Agricultural Policy Chinese Academy of Sciences Beijing 100101 China

As a renewable energy,biofuel has attracted great attention in china and the rest of *** with the national food security,china recently has shifted its biofuel development priority from grain-based to non-grain-based biofuels,including forest-based biodiesel,since *** curcas is one of major biodiesel ***,there is rising debate on availability of land for expanding Jatropha curcas *** overall goal of this paper is to evaluate potential land for Jatropha curcas used as feedstock for biodiesel in *** on remote sensing data on land use,data on meteorological,soil and land slope,and suitable environment for Jatropha curcas plantation,this study uses Agro Ecological Zone method and considers social-economic constraints to evaluate potential suitable land for Jatropha curcas plantation in china's major Jatropha curcas production region,southwest *** results show that while there are some potential lands to expand Jatropha curcas areas,amount of these lands will hardly meet the government's target for Jatropha curcas-based biodiesels development in the *** may need to reconsider its long-term targets on the development of Jatropha curcas-based biodiesels.

关键词： biodiesel Jatropha curcas potential land suitability evaluation southwest china

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HpLC in reversed phase Mode:Tool for Investigation of Isoquercitrin from Enzymatic Biosynthesis products of rutin

HPLC in Reversed Phase Mode:Tool for Investigation of Isoque...

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2009 International Conference of Natural products and Traditional Medicine(ICNpTM’ 09)

作者： Jun Wang1,Fu-An Wu1,2 *,Lu-Lu Zhao1,Tao Wu2,Qing Yang3,Yao Liang1,Shui-Qin Fang1,Xing Jiang1,Meng Wang1 1college of Biological and Environmental Engineering,Jiangsu university of science and Technology,zhenjiang 212.18,p.r.china 2sericultural research institute,chinese academy of agricultural sciences,zhenjiang 212.18,p.r.china 3college of food science,southwest university,chongqing 400715,p.r.china

Isoquercitrin（quercetin 3-o-β-glucoside） can be obtained on a large scale by directional hydrolysis of rutin with hesperidinase,which has more physiologically potential application than rutin in a wide range of biological activities.A rapid and inexpensive HpLC method in reversed phase mode has been developed for the quantitative analysis of rutin and its enzymatic hydrolysis *** in HpLC-UV was on an Alltima C18 column（250mm×4.6mm i.d.;5μm） with a mobile phase consisting acetonitrile:0.02% phosphoric acid solution（20:80 v/v）,at a flow rate of 1.0 mL·min-1,detected at *** equations of rutin and isoquercitrin were y = 0.0000002x-19287（r2 =0.9973） and y= 0.0000002x-4648（r2 =0.9990）,which showed good linear relationships between the peak area of each marker and concentration,*** method has good repeatability and precision,the intraday and interday rSD for both retention time and peak area was less than 1.10%.The mean recoveries of rutin and isoquercitrin in samples,measured at three concentration levels,were 96.6 and 98.9%,their rSD were 2.1% and 1.4 %（n = 3）,*** biotransformation conversion rate of isoquercitrin was the highest when crude hesperidinase was used as *** niger LJ-11 was selected as producer and it was used to obtain the crude hesperidinase in submerged fermentation with added *** results indicated that the developed assay method was rapid,accurate,reliable and could be readily utilized as a quantitative analysis tool for investigation of isoquercitrin from enzymatic biosynthesis products of rutin.

关键词： HpLC isoquercitrin rutin enzymatic biosynthesis

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Artificial Tactile Sense Technique for predicting Beef Tenderness Based on FS pressure Sensor

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Journal of Bionic Engineering 2009年第2期6卷 196-201页

作者： Xiao-dan Wang~1,Yong-hai Sun~1,Ying Wang~2,Tie-jun Hu~3,Min-hua Chen~1,Bing He~31.School of Biological and agricultural Engineering,Jilin university,Changchun 130022,p.r.china2.School of food science and Engineer,Qingdao agricultural university,Qingdao 266109,p.r.china3.Jilin province Changchun Haoyue Halal Meat Co.,Ltd,Changchun 130013,p.r.china [a]School of Biological and agricultural Engineering Jilin University Changchun 130022 P. R. China [b]School of Food Science and Engineer Qingdao Agricultural University Qingdao 266109 P. R. China [c]Jilin Province Changchun Haoyue Halal Meat Co. Ltd Changchun 130013 P. R. China

We present a rapid system for predicting beef tenderness by mimicking the human tactile sense. The detection system includes a FS pressure sensor, a power supply conversion circuit, a signal amplifier and a box in which the sample is mounted. A sample of raw Longissimus dorsi (LD) muscle is placed in the measuring box; then a rod connected to the pressure sensor is pressed into the beef sample to a given depth; the reaction force of the beef sample is measured and used to predict the tenderness. Sensory evaluation and Warner-Bratzler Shear Force (WBSF) evaluation of samples from the same LD muscle are used for comparison. The new detection system agrees with established procedure 95% of the time, and the time to test a sample is less than 5 minutes.

关键词： bionics tactile sense beef tenderness detection FS pressure sensor

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Highly efficient transformation of intact yeast-like conidium cells of Tremella fuciformis by electroporation

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science china(Life sciences) 2008年第10期51卷 932-940页

作者： GUO LiQiong1,2, LIU Yong1, ZHAO ShuXian1, LIU ErXian1 & LIN JunFang1,2 1 college of food sciences, South china agricultural university, Guangzhou 510640, china 2 institute of Biomass research, South china agricultural university, Guangzhou 510640, china 1. college of food sciences South China Agricultural University Guangzhou 510640 China 2. institute of Biomass research South China Agricultural University Guangzhou 510640 China

Tremella fuciformis is one of higher basidiomycetes. Its basidiospore can reproduce yeast-like conidia, also called the blastospore by budding. The yeast-like conidia of T. fuciformis is monokaryotic and easy to culture by submerged fermentation similar to yeast. So it is a good recipient cell for exogenous gene expression. In this study, two expression vectors pGlg-gfp containing gpd-Gl promoter and gfp gene and pGlg-hph containing gpd-Gl promoter and hph gene were constructed. The lowest sensitive concentration of hygromycin for the blastospore was determined on three types of media. Our ex- periments showed that the lowest sensitive concentration of hygromycin for the blastospore was 5 μg/mL on MA medium. The intact blastospores were transformed with the expression vector pGlg-hph by electroporation. The putative transformants were obtained by the MA selective medium. Experi- mental results showed that the most effective parameters for the electroporation of intact blastospores were obtained by using STM buffer, 1.0×108 cells/mL of blastospores, 200 μL in transformation volume, 6 μg plasmid, 2.0 kV/cm of electric pulse voltage, stillness culturing on MB liquid medium for 48 h after electroporation. In these transformation conditions, the efficiency reached 277 colonies/μg DNA. Co-transformation of plasmid pGlg-gfp and pGlg-hph with ratio of 1:1 was performed by electroporation with the optimal parameters. The putative co-transformants were obtained by the MA selective medium. Eight randomly selected colonies from the vast putative co-transformants were analyzed by pCr de- tection and Southern blotting. The experiments showed that the gfp was integrated into the genomes of three transformants. The co-transformation efficiency was 37.5%. Green fluorescence was observed under laser scanning confocal microscope in these gfp positive transformants. This indicates that the exogenous gfp can be expressed effectively in the yeast-like conidia of T. fuciformis.

关键词： Tremella fuciformis electroporation yeast-like conidia intact cells gene expression hph gene gfp gene

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Efficient transformation and expression of gfp gene in the edible mushroom pleurotus nebrodensis

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progress in Natural science:Materials International 2008年第7期18卷 819-824页

作者： Junfang Lin a,b, Mingyao Zheng a, Jie Wang a,b, Wei Shu c, Liqiong Guo a,b,* a Department of Biotechnology, college of food sciences, South china agricultural university, Guangzhou 510640, china b institute of Biomass research, South china agricultural university, Guangzhou 510640, china c Test and Experimental Center, South china agricultural university, Guangzhou 510640, china Department of Biotechnology College of Food Sciences South China Agricultural University Guangzhou 510640 China institute of Biomass research South China Agricultural University Guangzhou 510640 China Test and Experimental Center South China Agricultural University Guangzhou 510640 China

An efficient transformation method mediated by pEG-protoplasts was developed for the newly commercial edible mushroom pleu- rotus nebrodensis. Two plasmids were used to co-transform protoplasts of p. nebrodensis. One plasmid is pAN7-1 containing a positive selectable marker gene hph conferring hygromycin B resistance. Another plasmid is pBlue-GFp containing a reporter gene gfp conferring green fluorescent protein. pCr and Southern blot analysis showed that hph gene or/and gfp gene were integrated into the genome of p. nebrodensis transformants. The transformation efficiency of the positive selectable marker gene hph was 3 transformants per microgram of plasmid pAN7-1 DNA, which was about 30 times higher than that previously reported in thoroughly studied pleurotus species such as pleurotus ostreatus. The transformation efficiency of the reporter gene gfp was 9 transformants per microgram of plasmid pBlu-GFp DNA. The co-transformation efficiency was 23.68%. This is the first report that a "reporter" gene, green fluorescent protein gene can be successfully stably expressed in this pleurotus species.

关键词： pleurotus nebrodensis protoplast-mediated transformation Green fluorescent protein GFp expression

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EST-derived microsatellites in p seudosciaena crocea and their applicability to related species

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Acta Oceanologica Sinica 2010年第6期29卷 83-91页

作者： YE Hua WANG Xiaoqing GAO Tianxiang WANG Zhiyong college of Animal science and Technology Hunan Agricultural University Changsha 410128 China Key Laboratory of science and Technology for Aquaculture and food Safety Fisheries College/Fisheries Biotechnology Institute Jimei University Xiamen 361021 China Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 China

Simple sequence repeat （SSr） markers were obtained for the large yellow croaker pseudosciaena crocea using 1 205 expressed sequences tags （ESTs） from the NCBI *** for 48 ESTSSr loci were designed and screened with 30 *** specimens captured from Guanjingyang sea area in Fujian province of *** of the loci were polymorphic,which were amplified with 3 to 11 alleles per locus and the mean of *** observed and expected heterozygosity per locus ranged from 0.091 to 0.844 （mean 0.544） and from 0.118 to 0.892 （mean 0.644）,*** information content （pIC） ranged from 0.115 to 0.866 （mean 0.593）.The results for cross-species amplification of the 16 large yellow croaker EST-SSrs on ***,***,***,*** and *** revealed that 14,12,11,7 and 6 loci were successfully amplified with 1 to 10 alleles with an average of 4.5 per locus,respectively,which are suitable for population genetics studies of these species and useful for phylogenetic relationship analysis among these ***,this study provides a set of type I markers for population genetics studies and genome mapping for large yellow croaker and its closely related species.

关键词： pseudosciaena crocea EST-SSrs genetic diversity cross-species amplification

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Dietary Methionine Affect Meat Qulity and Myostatin Gene Exon 1 region Methylation in Skeletal Muscle Tissues of Broilers

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agricultural sciences in china 2010年第9期9卷 1338-1346页

作者： LIU Guo-qing , ZONG Kai, ZHANG Li-li and CAO Shu-qing School of Biotechnology and food Engineering, Hefei university of Technology, Hefei 230009, p.r.china [a] School of Biotechnology and food Engineering Hefei University of Technology Hefei 230009 P.R. China

Dietary amino acids imbalance will result in stunted broiler performance and deteriorated meat quality, which are involved in various biochemical cycles in vivo. In this study, the effects of dietary methionine on meat quality and methylation of myostatin exon 1 were investigated. Drip loss of the broilers fed with diet of high methionine levels （0.2%） increased from （6.3 ± 0.1）% （control group） to （10.1 ± 1.0）%, and the muscle shearing force increased from （22.8 ± 1.9） N （control group） to （26.3 ±2.3） N. Moreover, many CpG sites were found at the myostatin exon 1 region （nucleotides 2 360-2 540 bp）. To further understand the regulation of broiler myostatin expression, the methylation status of broiler myostatin exon 1 and its mrNA expression were analyzed. At the myostatin exon 1 region where CG enriches （nucleotides 2 360-2 540 bp）, the percentages of methylation were 46 and 84% in low Met and high Met content groups after 55-d feeding, respectively. In skeletal muscle tissues, the exon 1 hypermethylation status of myostatin gene was found to be negatively correlated with the gene expression. These results suggested that methylation of this gene is a dynamic process, which plays a dominant role in regulating gene expression for development of individuals.

关键词： DNA methylation gene expression meat quality methionine myostatin

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Spatial-Temporal Changes in Grain production, Consumption and Driving Mechanism in china

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Journal of Integrative Agriculture 2013年第2期12卷 374-385页

作者： XU Shi-wei WU Jian-zhai SONG Wei LI Zhi-qiang LI Zhe-min KONG Fan-tao Key Laboratory of Digital agricultural Early-Warning Technology Ministry of Agriculture/Agriculture Information Institute Chinese Academy of Agriculture Sciences institute of Geographic sciences and Natural resources research Chinese Academy of Sciences

The spatial-temporal patterns of grain production and consumption have an important influence on the effective national grain supply on condition of tight balance in the total grain amount in china. In this paper, we analyze the spatial-temporal pattems of grain production, consumption and the driving mechanism for their evolution processes in china. The results indicate that both gravity centers of grain production and consumption in china moved toward the northern and eastern regions, almost in the same direction. The coordination of grain production and consumption increased slightly from 1995 to 2007 but decreased from 2000 to 2007. There is a spatial difference between the major districts of output increase and the strong growth potential in grain consumption, which indicates an increasing difficulty in improving the regional coordination of grain production and consumption. The movement of the gravity center of grain production is significantly correlated with regional differences in grain production policy, different economic development models, and spatial disparity of land and water resource use. For grain consumption, the main driving factors include rapid urbanization, the upgrade of food consumption structure, and distribution of food industries.

关键词： grain production grain consumption gravity center driving mechanism

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Expression and function of heregulin-α and its receptors in the mouse mammary gland

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science china(Life sciences) 2010年第8期53卷 1015-1024页

作者： GENG LiJing1 & LI QingZhang2 1institute of food science and Engineering, Liaoning Medical university, Jinzhou 121000, china 2Key Laboratory of Dairy science of Ministry of Education, Northeast agricultural university, Harbin 150030, china 1. institute of food science and Engineering Liaoning Medical University Jinzhou 121000 China2. Key Laboratory of Dairy Science of Ministry of Education Northeast Agricultural University Harbin 150030 China

Heregulin-α (HrGα) is a cytokine secreted by the mammary mesenchyme, adjacent to lobuloalveolar structures. To understand the role of HrGα and its receptors in mammary glands, and the underlying mechanisms, we performed this study to determine the expression and localization of HrGα and its receptors ErbB2 and ErbB3. We also determined the role of HrGα in the development of mammary glands, β-casein expression and secretion, rab3A protein expression and the phosphorylation of HrGα signaling molecules using confocal laser scanning microscopy, tissue culture, capillary electrophoresis, Western blotting and enzyme-linked immunosorbent assays. We found that a peak was on pregnancy day 15. Changes of ErbB2 and ErbB3 expression were positively and linearly correlated with HrGα, indicating that HrGα positively regulates ErbB2 and ErbB3 expression. During pregnancy, HrGα enhanced the phosphorylation of STAT5, p42/p44, p38, pKC and rab3A protein expression, stimulated the proliferation and differentiation of the ductal epithelial cells of mammary glands, and increased and maintained the expression and secretion of β-casein. During lactation, HrGα enhanced the phosphorylation of STAT5 and p38, inhibited the phosphorylation of pKC and rab3A protein expression, maintained the morphology of the mammary glands and increased the secretion of lactoprotein to reduce the expression of β-casein in mammary epithelial cells. During involution, HrGα induced the phosphorylation of STAT3 and rab3A protein expression, and inhibited the phosphorylation of pKC to stimulate the degeneration of mammary epithelial cells. It also inhibited the secretion of β-casein, resulting in increased levels of β-casein in mammary epithelial cells.

关键词： mammary gland heregulin-α ErbB2 ErbB3 signal transduction

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Methyl Jasmonate reduces Chilling Injury and Maintains postharvest Quality in peaches

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agricultural sciences in china 2003年第11期2卷 1246-1252页

作者： FENG Lei, ZHENG Yong-hua, ZHANG Yan-fen, WANG Feng ZHANG Lan and LU Zhao-xin(college of food science and Technology, Nanjing agricultural university, Nanjing 210095 , p.r.china) college of food science and Technology Nanjing Agricultural University Nanjing 210095 P. R. China

Zhaohui peaches(prunus persica Batsch)were treated with 0(CK), 1, 10 or 100 μmol L-1 methyl jasmonate(MeJA)vapor at 20℃ for 24 h before stored at 0℃ for 35 d. The untreated fruits showed chilling injury(CD symptoms after 4 wk of storage, as indicated by increased fruit firmness and reduced ex-tractable juice, which is referred as leatheriness. Treatment with 1 and 10 μmol L-1 MeJA promoted normal ripening and softening, maintained higher levels of extractable juice, titratable acidity, pectinesterase(pE)and polygalacturonase(pC)activities, inhibited increases in fruit fresh weight loss, decay incidence, electrolyte leakage and MDA content, and improved color development, thereby preventing chilling injury symptoms development and maintaining edible quality. MeJA treatment also delayed the climacteric rise in respiratory rate, but promoted ethylene production during the later period of cold storage, suggesting that ethylene may involve in CI development of peaches. These results indicate that 1 and 10 μmol L-1 MeJA treatments could be used to reduce CI development and decay incidence in peaches.

关键词： peach fruit Chilling injury Leatheriness Quality Methyl jasmonate

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