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Factors Influencing Phalaenopsis Tansformation by Particle Bombardment

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Factors Influencing Phalaenopsis Tansformation by Particle B...

2012年环境污染与大众健康学术会议

作者： Shuguo Fan 1,2 1 Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong, 675000 2 Institute for Bio-resources Development and utilization in Central Yunnan Plateau, Chuxiong, 675000

An efficient and reproducible gene transformation system from PLBs of Phalaenopsis amabilis has been created for the molecular breeding of orchids. L-methionine sulfoximine （MSO） was used as an agent for selection of transgenic Phalaenopsis, and the bialaphos resistance （bar） gene as a selectable marker. Gene transformation was performed by particle bombardment with a 4-day recovery period on MSO-free medium and two selection stages on media with increasing amounts of selection agent, using concentrations of 0.5 and 2 μM MSO. Helium gas pressure of 1350 psi and a distance of 9 cm from the stopping screen to orchid PLBs on plates were optimal parameters for biolistic bombardment of P. amabilis, as transformation under these parameters gave the highest number of putative transformants after 3 months of MSO selection. Independent transgenic lines were obtained and the presence of the transgene was confirmed by PCR and PCR Southern blot analysis, respectively.

关键词： Cymbididum mosaic virus (CymMV) Phalaenopsis amabilis coat protein (CP)

efficient Synthesis of 1,3-Disubstitutedimidazole-2-selones

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Efficient Synthesis of 1,3-Disubstitutedimidazole-2-selones

第一届硒与环境和人体健康国际会议

作者： R Li X.F.Wang X.H.Yuan S.D.Wang and S.W.Lu~* national engineering research center for Catalysis Dalian Institute of Chemical Physics Chinese Academy of Sciences 457 Zhongshan Road Dalian Liaoning 116011 P.R.china.

＜正＞Introduction Chemistry of imidazole-2-selone derivatives has attracted great attention recently because:（1） the imidazole-2-selone derivatives could serve as donors towards halogens in a fashion similar to many other selone derivatives,affording charge-transfer complexes of spoke structures or T-shaped structures;（2） some of the imidazole-2-selone derivatives are bioactive and promising *** have also reported that some of imidazole-2-selones are efficient catalysts for oxidative carbonylation of organic *** their important chemistry synthetic methodologies to imidazole-2-selone derivatives have not been extensively *** route to 1,3-disubstitutedimidazole-2-selone derivatives was to react elemental Selenium（Se） with the N-heterocyclic carbenes（NHCs） prepared

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A Technique of Triangular Mesh Adaptation and Its Application

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A Technique of Triangular Mesh Adaptation and Its Applicatio...

2011 International Conference on Business Management and Electronic Information(BMEI 2011)

作者： Wang Xueyuan School of Information engineering Southwest University of Science and technology Mianyang Zhang Zhengyu china Aerodynamics research and Development center Mianyang He Xiaochun Sichuan Jiuzhou Electronic Technology CO.LTD Mianyang

A method of triangular mesh adaptation and its application is presented in this paper.A triangular mesh is divided into multi-region according to its geometrical feature,and its triangles are sorted by their curvature and pressure gradient for the operations of refinement and *** from optimizing vertex position by mapping triangular mesh 3D to 2D regular region,the techniques of maximizing minimum angle and vertex relocation based on surface reconstructing are employed in this paper for every triangle of each region,to realize robust and efficient adaptation for a triangular *** method is especially suitable to generate triangular mesh for numerical calculation from STL model with free-form surface,and it is verified by some engineering cases,which shows this method is useful.

关键词： remeshing triangular mesh optimization of triangular mesh

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一种高效和可扩展的OCSP系统

通信学报 2003年第11期24卷 93-99页

作者：周永彬卿斯汉季庆光张振峰中国科学院软件研究所中国科学院信息安全技术工程研究中心北京100080 信息安全国家重点实验室北京100080

证书状态查询是PKI中的一个关键问题,OCSP是解决这个问题的一种重要机制。本文分析了OCSP协议的技术细节,并在此基础上设计了一种高效的、可扩展的OCSP系统。文中对该系统的关键技术和其自身的安全性问题进行了详细的论述。最后,给出了... 详细信息

证书状态查询是PKI中的一个关键问题,OCSP是解决这个问题的一种重要机制。本文分析了OCSP协议的技术细节,并在此基础上设计了一种高效的、可扩展的OCSP系统。文中对该系统的关键技术和其自身的安全性问题进行了详细的论述。最后,给出了关于OCSP机制的一些未决问题以及某些思考。

关键词：公钥基础设施 OCSP系统证书状态查询认证中心

Randomized Algorithms for Optimal Robust Performance Controller Design

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Randomized Algorithms for Optimal Robust Performance Control...

第十九届中国控制会议

作者： Chunlei Song Long Wang Lin Huang center for Systems and Control Department of Mechanics and engineering Science Peking University, Beijing, 100871, P.R.china

＜正＞In this paper efficient randomized algorithms are constructed to solve optimal robust performance controller design problems. The randomized algorithms here are based on a property from statistical learning theory known as uniform convergence of empirical means （UCEM）. It is argued that in order to assess the performance of a controller as the plant varies over a prespecified family, it is better to use the average performance of the controller as the objective function to be minimized, rather than its worst-case performance. The approach is illustrated to be efficient through an example.

关键词： randomized algorithms statistical learning theory optimal robust performance controller design

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消能减震建筑结构的计算分析

西北建筑工程学院学报（自然科学版） 2002年第1期19卷 9-14,19页

作者：吴泽厚周福霖深圳宝安工程质量监督检验站广东深圳518101 广州大学工程结构抗震研究中心广东广州510800

运用非线性规划中的复形法 ,对消能支撑框架结构中消能支撑的参数进行了优化 ,编制了适用性较好的计算机程序 ,并以一个 6层钢筋混凝土框架作为算例。

关键词：减震建筑结构计算消能支撑框架复形法

Safflower as bioreactor produce Acid Fibroblast Growth Factor(aFGF)

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Safflower as bioreactor produce Acid Fibroblast Growth Facto...

第十三届全国植物基因组学大会

作者： Zhenhao Guan~#,Jing Yang~#,Yongxin Guo,Yanfang Wang,Xiuming Liu,Haiyan Li~*,Xiaokun Li~* engineering research center of Bioreactor and Pharmaceutical Development,Ministry of Education,Jilin Agricultural University,Changchun,jilin 130118,china

Plant is safe,efficient and low cost to produce medical value proteins,peptides or antibodies and oral *** as a bioreactor to produce aFGF,which could not only cumulate the activity in tissue wound repair of aFGF and the functions of safflower which conclude activating blood circulation,eliminating stasis to activate blood circulation,relieving the pain,and to heal the injury from *** oleosin gene

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束晕-混沌的复杂性理论与控制方法及其应用前景

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物理学进展 2003年第3期23卷 321-388页

作者：方锦清陈关荣中国原子能科学研究所北京27581信箱102413 香港城市大学混沌控制与同步学术研究中心

　本文系统论述涉及强流加速器等强流离子束装置中产生的束晕混沌的复杂性理论与控制方法及其应用前景。强流离子束在核材料生产与增殖、洁净核能、放射性废物嬗变、放射性药物生产、重离子聚变、高能物理、核科学与工程、国防与民用... 详细信息

　本文系统论述涉及强流加速器等强流离子束装置中产生的束晕混沌的复杂性理论与控制方法及其应用前景。强流离子束在核材料生产与增殖、洁净核能、放射性废物嬗变、放射性药物生产、重离子聚变、高能物理、核科学与工程、国防与民用工业和医疗等许多方面都有极其重要的应用潜力和诱人的发展前景。尤其是,近年来强流加速器驱动的放射性洁净核能系统是国内外关注的热门课题,因为它比常规核电更安全、更干净、更便宜。但是,强流离子束形成的束晕混沌的复杂性现象已引起了国内外广泛关注,需要加以抑制、控制和消除这类现象,解决这一难题已经成为强流离子束应用中的关键问题之一。目前不仅必须深入研究这类束晕混沌的复杂特性及其产生的物理机制,而且需要研究如何实现对束晕混沌的有效控制,并寻求和发展其新理论、新方法和新技术。这就向强流离子束物理和非线性-复杂性科学及其技术提出了一系列极富挑战性的新课题。本文结合国内外的研究概况,根据我们多年来的研究成果,特别是我们首创性地提出了一些束晕混沌的有效控制方法,它们包括:非线性反馈控制法,小波反馈控制法,变结构控制法,延迟反馈控制法,参数自适应控制法等,进行重点的介绍。

关键词：束晕-混沌控制复杂性理论控制方法强流加速器等强流离子束装置放射性洁净核能系统

resources Investigation and Ribosomal DNA ITS Sequence Analyses of Medical Plant of Rumex hastatus

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Resources Investigation and Ribosomal DNA ITS Sequence Analy...

中华中医药学会第九届中药鉴定学术会议

作者： ZHENG Shui-qing~1,ZENG Ming~2,CHEN Wan-sheng~1,MA Ya-jun~3,Ding Ru-xian~1,QIAO Chuan-zhuo~1,CAO Hui~4 (1.College of Pharmacy,the Second Military Medical University,Shanghai 200433,china 2.Department of Pharmacology, General Hospital of Beijing Military Area,Beijing 100700,china,3.Department of Basic Medicine of the Second Military Medical University,Shanghai 200433,china 4.national engineering research center for Modernization of Traditional Chinese Medicine,Zhuhai 519020,china)

Objective:To investigate the resources of medical plants of Rumex hastatus in china for providing scientific data for their exploitation and to sequence its ribosomal DNA ITS for providing DNA molecular markers for authenticating Rumex *** Field investigation,sample collecting and identifying,and specimens checking in some institutes of botany were carried *** DNA ITS was amplified by PCR using consensus primer set and its sequence was determined by PCR direct *** Rumex hastatus is growing in Yunnan、Sichuan,southeast of Tibet and west of Guizhou. resources of Rumex hastatus are relatively rich in above *** lengths of its internal transcribed spacer 1,5.8S ribosomal RNA gene and internal transcribed spacer 2 are respectively 199bp,165bp and *** Rumex hastatus has values of development and utilization and DNA sequencing could be a useful and reliable tool in the identification of Rumex hastatus.

关键词： Rumex hastatus resources investigation ribosomal DNA internal transcribed spacer