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沈阳药科大学学报 2008年第S1期25卷 81-82页

作者： JIN Yue1,LV Yong1,HAN Guo-zhu1,YU Hong-shan2,JIN Feng-xie2(1.department of Clinical Pharmacology,dalian medical university,dalian 116044 2.department of Foodstuffs and Bio-engineering,dalian Polytechnology university,dalian 116034)

Objective To comparatively study anti-free radical and cytoprotective effects of quercetin(Q)and its monoglucoside isoquercetin(I),diglucoside rutin(R),which differs only in glycosyl-substitution at C-3 position of the molecules,using anoxia/hypoglycemia-induced cell injury model and thereby to explore the structure-effect relationship *** The cell injury model was established by HEK293 cells cultured in vitro with Na2S2O3 plus sugar-free Earle's fluid as incubation medium;Cell survival rate(CSR),total antioxidant capacity(TAC),SOD and LDH levels were determined;The effect intensity of the 3 flavonoids were compared by means of IC50,the concentration required to achieve 50% inhibition of the changes in the above indices in injured *** Q,I and R all concentration-dependently elevated CSR,TAC and SOD,and reduced LDH level;the all of IC50s for the above indices were ranked in order of IC50,QI>*** The 3 structurally similar flavoloids all have significant and concentration-dependent anti-free radical and cyto-protective effects with the intensity being in order of aglycone>monoglucoside>diglucoside;the substitution of-OH by sugar group at C-3 position of flavoloids and increase in the sugar-substituent number are associated with the effect intensity reduced;namely,the intensity of these effects of flavonoids is negatively related the substutution by sugar group at C-3 position.

关键词： quercetin isoquercetin rutin flavonoid free-radical cytoprotection cell culture

Morphological and functional changes in the blood-spinal cord barrier of rabbits in an experimental spinal cord presyrinx state

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Neural Regeneration Research 2006年第7期1卷 597-600页

作者： Jianfeng Li1, Haiying Liu1, Qingjun Zhang2 1department of Neurosurgery, Fourth Hospital, Hebei medical university, Shijiazhuang 050011, Hebei province, china 2department of Neurosurgery, People’s Hospital, Peking university, Beijing 100044, china department of Neurosurgery Hebei Medical University Shijiazhuang 050011 Hebei Province China department of Neurosurgery People's Hospital Peking University Beijing 100044 China

BACKGROUND: Presyrinx state of spinal cord can reflect the initial lesion of syringomyelia (SM). The early trials has proved that ischamia and edema are main pathological changes of presyrinx state. OBJECTIVE: To establish SM model of rabbits for investigating the relationship between changes of morphous and function of blood-spinal cord barrier and the edema degree, histological changes in presyrinx state of SM, and to explore the mechanism of the presyrinx state of SM. DESIGN: Randomized controlled animal experiment. SETTING: department of Neurosurgery, Fourth Hospital, Heibei medical university. MATERIALS: Sixty Chinese healthy white rabbits, aged 3.5-4.5 months, weighing 1.5-2.0 kg, were provided by Experimental Animal Center of Hebei medical university [certification: (SYXK(Ji)2003-0026)]. Evan's blue (EB) and dimethylformamide (DMF) were purchased from Jingmei Biotech Co., Ltd. RM2125 paraffin section cutter (Leica Company, Japan), H-7500 transmission electron microscope (Hitachi Company, Japan), PM-20 light microscope photograph system (Olympus Company, Japan). METHODS: The experiment was carried out in the Laboratory of Neurosurgery department, Second Hospital of Hebei medical university from January to June 2006. ① All the rabbits were randomly divided into two groups: model group (n =40), control group (n =20). Rabbits in two groups were divided into five subgroups once again at five time points (1st, 3rd, 7th, 14th, 21st days, n =8 and n =4 at each time point in the model group and control group, respectively). Under ketamine anesthesia, 0.6 mL Kaolin solution (250 g/L, 37 ℃) was injected into the cisterna magna of rabbits in model group, while 0.6 mL physiological saline (37 ℃) was injected into the rabbits of control group. ② On the 1st, 3rd, 7th, 14th, 21st days after kaolin injection, cervical cord samples were harvested after sacrifice of animal. Quantitative analysis on the function of blood-spinal cord barrier was performed by Evan's blue technique

关键词： Morphological and functional changes in the blood-spinal cord barrier of rabbits in an experimental spinal cord presyrinx state

Interaction of cellular-localized signature modules in response to prostate cancer

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Progress in Natural Science:Materials International 2007年第11期17卷 1368-1375页

作者： Zhu Jing1, Guo Zheng1,2, Zhang Min2, Yang Da2, Wang Jing2 and Wang Chenguang2(1. School of Life Science and Bioinformatics Centre, university of Electronic Science and Technology of china, Chengdu 610054, china 2. department of Bioinformatics, Bio-Pharmaceutical Key Laboratory of Heilongjiang province, Harbin medical university, Harbin 150081, china) School of Life Science and Bioinformatics Centre University of Electronic Science and Technology of China Chengdu 610054 China department of Bioinformatics Bio-Pharmaceutical Key Laboratory of Heilongjiang Province， Harbin Medical University， Harbin 150081 China

Rapid progress in high-throughput biotechnologies (e.g. microarrays) and exponential accumulation of gene functional knowledge makes it promising for systematic understanding of complex human diseases at the functional modules level. Current modular categorizations can be defined and selected more specifically and precisely in terms of both biological processes and cellular locations, aiming at uncovering the modular molecular networks highly relevant to cancers. Based on Gene Ontology, we identifed the functional modules enriched with differentially expressed genes and characterized by biological processes and specific cellular locations. Then, according to the ranking of the disease discriminating abilities of the pre-selected functional modules, we further defined and filtered signature modules which have higher relevance to the cancer under study. Applications of the proposed method to the analysis of a prostate cancer dataset revealed insightful biological modules.

关键词： microarray analysis Gene Ontology classification analysis cellular location functional module

The cell cycle related apoptotic susceptibility to arsenic trioxide is associated with the level of reactive oxygen species

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Cell Research 2004年第1期14卷 81-85页

作者： Fei GAO~1, Jing YI~1, Jing Qi YUAN~2, Gui Ying SHI~1, Xue Ming TANG~(1,*) ~1department of Cell Biology, Shanghai Second medical university, Shanghai 200025, china ~2department of Automation, Shanghai Jiaotong university, Shanghai 200031, china. Tang XM Shanghai Med Univ 2 Dept Cell Biol Shanghai 200025 Peoples R China

Double staining flow cytometry was performed using 7-amino actinomycin D and 6-carboxy-2’,7’-dichlorodihydrofluorescein diacetate, to detect the level fluctuation of reactive oxygen species （ROS） during the cell cycle of normal NB4 cells. Our results showed that NB4 cells possessed higher level of ROS in G2/M phase than in G1 and S phases. Double staining flow cytometry, with TdT mediated dUTP nick end labeling （Tunel） and propidium iodide （PI）, indicated that As2O3 （2μM） could induce apoptosis in NB4 cells prevailingly from G2/M phase, and this efficacy was enhanced upon co-administration of 2, 3-dimethoxy-1, 4-naphthoquinone （DMNQ）（2.5μM） which could produce the endogenous ROS. These results suggested that different ROS level in different cell cycle phases of NB4 cells might determin the selective induction of G2/M apoptosis and the cells’ susceptibility to apoptosis by As2O3.

关键词： arsenic trioxide apoptosis cell cycle reactive oxygen species (ROS).

Relationship between collagen Ⅳ expression and biological behavior of gastric cancer

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World Journal of Gastroenterology 2000年第3期6卷 438-439页

作者： Zhen Ning Wang Hui Mian Xu Oncology department,china medical university,Shenyang 110001,liaoning province,china

INTRODUCTION Conceivably the presence of basement membrane(BM)in a neoplasm might be a result of interactionof tumor cells with the extracellular *** Ⅳ is one of the major intrinsic componentsof *** study has shown that collagen Ⅳhas cell adhesion function and is involved in theprocess of tumor invasion and metastasis,includingcolorectal cancer and breast *** there arefew systematic studies on gastric cancer and theresults are *** this study we evaluated

关键词： stomach neoplasms collagen IV biological behavior immunohistochemisty

Expression of prostaglandin E_2 receptors in rat hippocampus

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Neural Regeneration Research 2006年第7期1卷 668-669页

作者： Fang Liu1,2, Weidong Xu1, Xijiu Fu1, Jie Li1, Yanrong Song1, Xiaolin Li1, Xiaoxin He1, Dong Han1, Qiuxia Li1, Yuhua Chen3 1department of Neurology, Fengtian Hospital, Shenyang medical College, Shenyang 110024, liaoning province, china 2university of South Carolina, USA 3department of Development, china medical university, Shenyang 110024, liaoning province, china department of Neurology Fengtian Hospital Shenyang Medical College Shenyang 110024 Liaoning Province China university of South Carolina Columbia SC United States department of Development China Medical University Shenyang 110024 Liaoning Province China

BACKGROUND: Prostaglandin E2 (PGE2) can directly regulate toxic injury of hippocampal neurons through participation by its receptor. Increase of excitability of hippocampal membrane and long-term synaptic elasticity are closely related to PGE2, PGD2 and PGF2A. This suggests that PGE2 may be a key molecule of neuronal signal passage and regulate the existence of neurons through its receptor. However, which isoforms of PGE2 receptor expressing in hippocampal neurons is still unclear. OBJECTIVE: To research the subtype expression of PGE2 receptor in hippocampus of rats through mRNA transcription and protein interpretation. DESIGN: Animal studies with random, control and operator and designer double-blind methods. SETTING: university of South Carolina, Animal Center. MATERIALS: Sprague-Dawley rats, aged 12 weeks, weighing 200 g, females 48 and males 48, were selected from Animal Center in South Carolina university. Tri ReagentTM kit was provided by Molecular Research Center, USA. METHODS: The experiment was carried out in Animal Center in South Carolina university from January to December 2005. The expression of the PGE2 receptors was profiled and compared in rat hippocampus using real-time RT-PCR and Western-blot techniques. MAIN OUTCOME MEASURES: Expression of PGE2 receptors in various isoforms of hippocampal neurons of rats. RESULTS: mRNAs of all four EP1-4 subtypes were detected in the hippocampus. Western-blot data showed consistently detectable bands at approximately Mr 50 000 of EP1, Mr 40 000 and Mr 52 000 of EP2, Mr 45 000, Mr 57 000 and Mr 105 000 of EP3, and Mr 46 000 of EP4. CONCLUSION: Identifying four subtypes of EPs heterogeneously expresses in the hippocampus.

关键词： PCR Expression of prostaglandin E2 receptors in rat hippocampus PGE

Inactivation gating determines drug potency:a common mechanism for drug blockade of HERG channels

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Acta Pharmacologica Sinica 2004年第5期25卷 12-18页

作者： Bao-feng YANG1, Dong-hui XU2, Chao-qian XU, Zhe LI, Zhi-min DU, Hui-zhen WANG, De-li DONGdepartment of Pharmacology, Harbin medical university, Harbin 150086, china 2department of Pharmacy, Sun Yat-sen university, Guangzhou 510080, china department of Pharmacology Harbin Medical University department of Pharmacy Sun Yat-sen University

AIM: To determine the mechanisms of interactions between different drugs and HERG channels. METHODS:Various antiarrhythmic (dofetilide, quinidine, azimilide, RP58866) and non-antiarrhythmic (terfenadine, nicotine)agents were used on HERG channels expressed in Xenopus oocyte. Whole-cell voltage-clamp techniques wereused. RESULTS: All drugs produced concentration-dependent block of HERG current. The inhibition was mark-edly facilitated with voltage protocols favoring channel inactivation (eg, less negative holding potentials). Maneu-vers that weakened channel inactivation (eg, elevation of external K+), relieved HERG blockade by all drugs. Moreover,the inhibitory potency was reduced by at least 20-300 fold with varying compounds when rapid C-type inactivationwas removed by a mutation located between the transmembrane domains 5 and 6 (S631A). CONCLUSION: Theinactivation gating of HERG channels determines the blocking potency of drugs. This mechanism might be com-mon to drugs of various classes.

关键词： antiarrhythmic agents potassium channels mutation channel inactivation patch-clamp techniques oocytes Xenopus

Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-con-verting enzyme-1 synthesis

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Acta Pharmacologica Sinica 2007年第8期28卷 1116-1122页

作者： Chao TANG~1 An-hua WU~(1,3) Hong-li XUE~2 Yurl-jie WANG~1 ~1department of Neurosurgery,the First Affiliated Hospital,china medical university ~2department of Neurosurgery,General Hospital of Shenyang Military Command,Shenyang 110001,china. department of Neurosurgery the First Affiliated Hospital China Medical University Shenyang 110001 China department of Neurosurgery General Hospital of Shenyang Military Command Shenyang 110001 China

Aim:To investigate the effects of tanshinone ⅡA（Tan ⅡA）on the regulation ofthe production of endothelin（ET）-1（including large ET-1）,mRNA levels of ET-1,endothelin-converting enzyme-1（ECE-1）,endothelin-A receptor（ETA）andendothelin-B receptor（ETB）induced by TNF-α in rat brain microvascular endo-thelial cells（BMVEC）.Methods:The ET-1 release（including large ET-1）into theculture medium was determined by enzyme *** levels of ET-1,ECE- 1,ETA,and ETBmRNA were measured by *** receptor bind-ing was also ***:The induction of ET-1 release by TNF-α from cul-tured BMVEC was dose-dependently reduced by Tan IIA,but large ET-1 levelsprogressively increased in response to Tan ⅡA;the mRNA expression of ET-1 *** ⅡA also caused a decrease in ETAreceptor mRNA and ECE-1expression in a dose-dependent *** receptor binding was unal-tered in BMVEC stimulated with TNF-α alone or a combination of TNF-α and TanⅡ***:These findings suggest that Tan ⅡA may inhibit ET-1 produc-tion in TNF-α-induced BMVEC through the suppression of ECE-1 synthesis.

关键词： tanshinone IIA endothelin-1 TNF-α brain microvascular endothelial cells

STAT1 Antisense Oligonucleotides Attenuate the Proinflammatory Cytokine Release of Alveolar Macrophages in Bleomycin-Induced Fibrosis

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Cellular & Molecular Immunology 2005年第3期2卷 211-217页

作者： Xianming Fan~(1,3) Zengli Wang~2 ~1department of Respiratory Medicine,the Affiliated Hospital of Luzhou medical College,Luzhou 646000,china ~2department of Respiratory Medicine,West china Hospital,Sichuan university,Chengdu 610041,china ~3Corresponding to:Dr.Xianming Fan,department of Respiratory Medicine,the Affiliated Hospital of Luzhou medical College,Luzhou 646000,china. department of Respiratory Medicine the Affiliated Hospital of Luzhou Medical College Luzhou 646000 China. fxm129@***

To investigate the effect of signal transducers and activators of transcription 1 （STAT1） antisense oligonucleotides （ASON） on concentrations of TNF-α IL-8, NO secreted by alveolar macrophages （AMs） in bleomycin-induced rat pulmonary fibrosis, five adult female Wistar rats were intratracheally instilled with bleomycin. After 7 days, the rats were killed by right ventricle of heart exsanguinations under ketamine anaesthesia and bronchoalveolar lavage （BAL） was performed to obtain AMs. AMs were divided into four groups, treated with STAT1 ASON, STAT1 sense oligonucleotides （SON）, dexamethasone （DEX） and medium alone （control）, respectively. AMs and media were collected after culture for 36 h. The mRNA and protein expressions of STAT1 and ICAM-1 in AMs were detected by RT-PCR and ELISA, respectively. The concentrations of TNF-α IL-8, NO in cultured medium were detected. The STAT1 mRNA expression by AMs in the STAT1 ASON group was lower than those of AMs in the STAT1 SON group, the DEX group and the control group （p 〈 0.05）. Moreover, the STAT1 mRNA expression by AMs in the DEX group was also lower than those of AMs in the STAT1 SON group and the control group （p 〈 0.05）, but the STAT1 mRNA expression by AMs in the STAT1 SON group was not different from that of the control group （p 〉 0.05）. The protein expressions of STAT1 and ICAM-1 and the mRNA expression of ICAM-1 showed similar changes to the STAT1 mRNA expression by AMs. The concentrations of TNF-α IL-8, NO in cultured medium from STAT1 ASON group were lower than those from STAT1 SON, DEX and the control groups （p 〈 0.05）. Moreover, the concentrations of TNF-α IL-8, NO in cultured medium from DEX group were also lower than those from the control and STAT1 SON group （p 〈 0.05）, but no difference between STAT1 SON group and the control （p 〉 0.05）. The results suggest that STAT1 ASON could inhibit the secretion of TNF-α IL-8, NO in AMs, and STAT1 could become a target of treating pulmonary fibrosis. Cellular ＆ Molecul

关键词： STAT1 pulmonary fibrosis alveolar macrophage ASON

Correlative study on the cognitive dysfunction of patients with myasthenia gravis

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Neural Regeneration Research 2006年第8期1卷 744-746页

作者： Wenli Chen1, Wenmin Wang2, Yi Wang3 1department of Neurology, Yunnan First People’s Hospital, Kunming 650032, Yunnan province, china 2department of Neurology, First Affiliated Hospital of Kunming medical College, Kunming 650032, Yunnan province, china 3department of Neurology, Huashan Hospital Affiliated to Shanghai Fudan university, Shanghai 200040, china department of Neurology Yunnan First People's Hospital Kunming 650032 Yunnan Province China department of Neurology Hospital of Kunming Medical College Kunming 650032 Yunnan Province China department of Neurology Huashan Hospital Shanghai Fudan University Shanghai 200040 China

BACKGROUND: Myasthenia gravis (MG) is considered as a peripheral neuromuscular disorder. Some investigations demonstrate that MG affects central nervous system (CNS), and there are disorders in cognitive function, affection, sleep, mental status and others; however, there are also some different standpoints. Some questions are still disputed, such as whether or not MG exists in the CNS and cognitive function is impaired. DATA SOURCES: Using the terms of 'myasthenia gravis, neuropsychological test, memory, central, brain, cognitive dysfunction and so on', we searched the Medline database for the articles about CNS involved in MG, nerve and neuropsychology as well as cognitive function, which were published in the English language between January 1985 and December 2004. Meanwhile, we also searched CNKI database by inputting 'myasthenia gravis, neuropsychology and cognitive function' in Chinese for articles about involved neuropsychology and cognitive function which were published between October 1995 and June 2006 in the Chinese language. STUDY SELECTION: Data were checked in the first trial, and articles about the influences of MG on CNS, neuropsychology, cognitive function and central cholinergic function were selected, then those were obviously unrelated with above criteria were excluded. Articles which expound the influences of MG on neuropsychology and cognitive were included, but those with repeated contents were also excluded. DATA EXTRACTION: A total of 89 related English articles and 34 Chinese articles were collected, including 43 studies on involved CNS and 32 studies on involved central cholinergic function and cholinergic receptor. Sixty-three literatures were about neuropsychology and cognitive function, which were closely related with this article. Seventy-two articles met inclusive criteria and 66 were excluded. Thirty of seventy-two were chosen for analysis. DATA SYNTHESIS: MG is an autoimmune disease of muscular diseased fatigue induced by transmissi

关键词： cognitive function central nervous system cholinergic receptor autoimmune disease English language Chinese language used in