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Caveolin-1 gene silencing promotes the activation of PI3K/AKT dependent on Erα36 and the transformation of MCF10A^(CE)

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Science china(Life Sciences) 2010年第5期53卷 598-605页

作者： FENG Shuang1,WANG Yang1,WANG Xi1,WANG ZhaoYi3,CUI YuYing1,LIU Jing4,ZHAO ChunHui1,JIN Mei1,2 & ZOU Wei1,2 1 College of Life Science,liaoning Normal university,dalian 116029,china 2 liaoning Key Laboratory of Biotechnology and Molecular Drug R&D,dalian 116029,china 3 Cancer Center,Creighton university,2500 California Plaza,Omaha,NE 68178,USA 4 First Clinical Hospital of dalian medical university,dalian 116011,china 1. College of Life Science Liaoning Normal University Dalian 116029 China3. Cancer Center Creighton University 2500 California Plaza Omaha NE 68178 USA4. First Clinical Hospital of Dalian Medical University Dalian 116011 China2. Liaoning Key Laboratory of Biotechnology and Molecular Drug R&D Dalian 116029 China

ERα36,a variant of estrogen receptor-α,acts as a dominant-negative factor in both estrogen-dependent and estrogen-independent transactivation signaling pathways,and is a key factor in the promotion,progression and prognosis of breast ***-1,a 22-to 24-kD integral membrane protein,may function as a tumor suppressor in inhibiting of many growth-promoting signaling *** was shown that downregulation of Caveolin-1 strengthens the interaction of ERα and *** conclusion,Caveolin-1 gene silencing activated the PI3K/AKT signaling pathway in an ERα36-dependent *** finding may provide a promising therapeutic target of breast cancer.

关键词： Caveolin-1 Estrogen receptor PI3K/AKT

Numerical Simulation and Experimental Validation of Electrokinetic Injection for Integrated Capillary Electrophoresis Chip

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稀有金属材料与工程 2006年第S3期 552-555页

作者： Yan Weiping~1 Li Henan~1 Chen Liming~1 Chen Haijun~2 (1.Dept.of Electronic Engineering,dalian university of Technology,dalian 116024,china) (2.Center of Analyse and Test,dalian medical university,dalian 116027,china)

It is the key element in the integrated capillary electrophoresis chip separation to push a certain liquid sample into the separation *** electrokinetic injection system model was set up;the theory and the rule of the liquid flow were *** numerical simulation describing the potential,velocity vector and the stream distribution in the micro channel is *** to these simulation results,the pushing sample injection experiments were designed and the experimental results agree well with the simulation results.

关键词： capillary electrophoresis electric potential distribution velocity vector distribution stream distribution

ERK1/2 contributes negative regulation to STAT3 activity in HSS-transfected HepG2 cells

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Cell Research 2004年第2期14卷 141-147页

作者： Ze Jun TIAN~2, Wei AN~(1,*) ~1department of Cell Biology, Capital university of medical Science, 10 Xitoutiao, You An Men Beijing 100054, china. ~2department of Cardiology, Second Hospital, Hebei medical university, Shijiazhuang, china.. 2department of Cardiology Second Hospital Hebei Medical University Shijiazhuang China.. 1department of Cell Biology Capital University of Medical Science 10 Xitoutiao You An Men Beijing 100054 China.

Signal transducer and activator of transcription 3 (STAT3) is a recently characterized transcription factor which is essential to liver regeneration. We have previously reported that hepatic stimulator substance (HSS), a novel growthpromoting substance, phosphorylated the epidermal growth factor (EGF) receptors and activated downstream Ras-MAP kinase (extracellular signal-regulated kinases, ERK1/2) cascade. However, whether HSS signal is related to STAT3 pathway remains unclear. The present study is aiming to explore the regulatory effect of activation of ERK1/2 evoked by HSS on STAT3 phosphorylation and STAT3 signaling. Human hepatoma cell line HepG2 was stably transfected with HSS cDNA and HSS expression was measured by Northern blot. The results showed that the transfection of HSS into HepG2 resulted in remarkable increase in cellular proliferation as compared with the non-transfected cells, and it was further proved that the cellular proliferation in the HSS-transfected cells was related to ERK1/2 a

关键词： hepatic stimulator substance ERK1/2 STAT3 hepatocyte growth.

Hepatic blood inflow occlusion without hemihepatic artery control in treatment of hepatocellular carcinoma

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World Journal of Gastroenterology 2010年第46期16卷 5895-5900页

作者： Shan Jin,department of General Surgery,Affiliated Hospital of Inner Mongolia medical College,Hohhot 010050,Inner Mongolia Autonomous Region,china Chao-Liu Dai,department of Hepatobiliary Surgery,Shengjing Hospital,china medical university,Shenyang,liaoning province,china department of General Surgery Affiliated Hospital of Inner Mongolia Medical College Hohhot 010050 Inner Mongolia Autonomous Region China. jinshangood@***

AIM:To investigate the clinical significance of hepatic blood inflow occlusion without hemihepatic artery control (BIOwHAC) in the treatment of hepatocellular carcinoma (HCC).METHODS:Fifty-nine patients with HCC were divided into 3 groups based on the technique used for achieving hepatic vascular occlusion:group 1,vascular occlusion was achieved by the Pringle maneuver (n=20);group 2,by hemihepatic vascular occlusion (HVO) (n=20);and group 3,by BIOwHAC (n=19).We compared the procedures among the three groups in term of operation time,intraoperative bleeding,postoperative liver function,postoperative complications,and length of hospital ***:There were no statistically significant differences (P > 0.05) in age,sex,pathological diagnosis,preoperative Child's disease grade,hepatic function,and tumor size among the three *** intraoperative complications or deaths occurrred,and there were no significant intergroup differences (P > 0.05) in intraoperative bleeding,hepatic function change 3 and 7 d after operation,the incidence of complications,and length of hospital *** and Pringle maneuver required a significantly shorter operation time than HVO;the difference in the serum alanine aminotransferase or aspartate aminotransferase levels before and 1 d after operation was more significant in the BIOwHAC and HVO groups than in the Pringle maneuver group (P < 0.05).CONCLUSION:BIOwHAC is convenient and safe;this technique causes slight hepatic ischemia-reperfusion injury similar to HVO.

关键词： Hepatic blood inflow occlusion without hemihepatic artery control Hepatocellular carcinoma Intraoperative bleeding Ischemia-reperfusion injury

ANGIOTENSIN Ⅱ: ANTAGONISM TO ELECTROACUPUNCTURE ANALGESIA AND INVOLVEMENT IN ELECTROACUPUNCTURE TOLERANCE IN PERIAQUEDUCTAL GREY OF THE RABBIT

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针刺研究 1989年第Z1期 145-146页

作者： Wang Xiaomin Han Jisheng department of Physiolongy, Beijing medical university,Beijing department of pathophysiology, dalian medical College, dalian

Male rabbits of 2. 0-2. 6kg was used. Nociception was assessed by measur- ing the latency of the escape response (ERL) elicited by strong radiant heatfocused on the skin of the nostrils. Eleetroacupuncture (EA) stimulation wasapplied via acupuncture needles inserted into the acupoint Zusanli and Qunlun,located near the knee joint and ankle joint respectively. The analgesic effectof EA (2-15Hz, 2V, 20min) could be blocked by injecting angiotensin Ⅱ (AⅡ)

关键词： PAG EA ANGIOTENSIN ANTAGONISM TO ELECTROACUPUNCTURE ANALGESIA AND INVOLVEMENT IN ELECTROACUPUNCTURE TOLERANCE IN PERIAQUEDUCTAL GREY OF THE RABBIT

Changes in Autoantibodies against β1-Adrenoceptor and M2-Muscarinic Receptor during Development of Renovascular Hypertension in Rats

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 793-797页

作者： Jian-Ming ZHI*, Rong-Fang CHEN1, Heng WU2, Hui-Rong LIU2, and Rong-Rui ZHAO2 department of Physiology, Shanghai Second medical university, Shanghai 200025, china 1department of Toxicology, Second Military medical university, Shanghai 200433, china 2 department of Physiology, Shanxi medical university, Taiyuan 030001, china department of Physiology Shanghai Second Medical UniversityShanghai 200025 China department of Toxicology Second Military Medical UniversityShanghai 200433 China department of Physiology Shanxi Medical UniversityTaiyuan 030001 China

In an experimental rat’s renovascular hypertension model, we studied the genesis of anti- cardiac β1-adrenoceptor and M2-muscarinic receptor autoantibodies in relation to the changes in immunological function during the development of renal hypertension. The biological activities of these autoantibodies were also examined. It was shown that after two weeks of operation both the frequency of occurrence and the titre of autoantibodies to cardiac β1-adrenoceptor and M2-muscarinic receptor were significantly increased as compared with the control of pre-treatment. The increased autoantibodies lasted for several weeks and then automatically decreased gradually to the pre-clipping level at 10 weeks. Meanwhile the ratio of CD4 /CD8 + + was also undergone an initial increase followed by gradual recovery and correlated well with the changes in antibody titre. The biological effects of these autoantibodies displayed an “agonistic-like” activities on the beating frequency of cultured neonatal cardiomyocyte. It is suggested that autoimmune mechanisms are involved in the pathogenesis of renal hypertension and the cardiac receptor autoantibodies might be one of the mechanisms leading to cardiac dysfunction.

关键词： autoantibody renal hypertension cardiac receptor immunology

Removal of Endotoxin from Human Serum Albumin Solutions by Hydrophobic and Cationic Charged Membrane

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Chinese Chemical Letters 2000年第4期11卷 357-360页

作者： Gui Lin WEI~1 Zhen Hua SHAN~1 Ming Chen PAN~2 Zhi Hong GAO~2 (1dalian Institute of Chemical Physics. Chinese Academy of Sciences. dalian 1 16012 2 dalian medical university 1st Teaching Hospital) Chinese Acad Sci Dalian Inst Chem Phys Dalian 116012 Peoples R China dalian Med Univ Teaching Hosp 1 Dalian Peoples R China

A novel matrix of macropore cellulose membrane was prepared by chemical graft, and immobilized the cationic charged groups as affinity ligands. The prepared membrane Fan be used for the removal of endotoxin from human serum albumin (HSA) solutions. With a cartridge of 20 sheets affinity membrane of 47 mm diameter, the endotoxin level in HSA solution can be reduced ro 0.027 eu/mL. Recovery of HSA was over 95%.

关键词： hydrophobic and cationic charged membrane endotoxin removal

Salvianic acid A inhibits induction of inflammatory mediators by blocking Nuclear Factor-κB activation in macrophages

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沈阳药科大学学报 2008年第S1期25卷 104-105页

作者： YUAN Jun,YAO Ji-hong,ZHOU Qin(dalian medical university,dalian 116044,china)

Objective To investigate the anti-inflammation effect and possible mechanism of Salvianic acid A(SAA)in mouse peritoneal *** Peritoneal macrophages were obtained from BALB/c *** induced nitric oxide(NO),tumor necrosis factor-alpha(TNF-α)and interleukin-6(IL-6)in supernatant,protein expression of inducible nitric oxide synthase(iNOS),matrix metalloproteinase-9(MMP-9)and activation of nuclear factor-kappa B(NF-κB)in the extract were *** SAA strongly inhibited the excessive production of NO,TNF-α and IL-6 in LPS-induced peritoneal macrophages in a concentration-dependent manner and blocked the expression of iNOS and *** with LPS alone increased the translocation of NF-κB(p65)from cytosol to the nucleus,but the SAA inhibited the translocation of NF-κB(p65).Conclusions The results showed that SAA had strong anti-inflammatory effects in LPS-stimulated peritoneal *** important mechanism is due to its inhibition of NF-κB activation.

关键词： salvianic acid a tumor necrosis factor-α nterleukin-6 inducible nitric oxide synthase matrix metalloproteinase-9 nuclear factor-kappa B

Cloning and Expression Analysis of p26 Gene in Artemia sinica

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Acta Biochimica et Biophysica Sinica 2007年第5期39卷 351-358页

作者： Lijuan JIANG~1, Lin HOU~(1*), Xiangyang ZOU2, Ruifeng ZHANG~1, Jiaqing WANG~1, We njing SUN~1, Xintao ZHAO~1, and Jialu AN~1 1 College of Life Sciences, liaoning Normal university, dalian 116029, china 2 department of Biology, dalian medical university, dalian 116027, china CollegeofLifeSciences LiaoningNormalUniversityDalian116029China departmentofBiology DalianMedicalUniversityDalian116027China

The protein p26 is a small heat shock protein that functions as a molecular chaperone to protect embryos by preventing irreversible protein damage during embryonic development. A 542 bp fragment of the p26 gene was cloned and sequenced. The fragment encoded 174 amino acid residues and the amino acid sequence contained the α-crystallin domain. Phylogenetic analysis showed that eight Artemia populations were divided into four major groups. Artemia sinica (YC) belonged to the East Asia bisexual group. Expression of the p26 gene at different developmental stages ofA. sinica was quantified using real-time quantitative polymerase chain reaction followed by cloning and sequencing. The relationship between the quantity of p26 gene expression and embryonic development was analyzed. The results indicated that massive amounts of p26 were expressed during the development of A. sinica. At the developmental stage of 0 h, A. sinica expressed the highest level of p26. As development proceeded, expression levels of the p26 gene reduced significantly. There was a small quantity of p26 gene expression at the developmental stages of 16 h and 24 h. We concluded that p26 might be involved in protecting the embryo from physiological stress during embryonic development.

关键词： Artemia sinica cloning expression p26 gene real-time quantitative PCR