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Journal of Huazhong University of Science and Technology(medical Sciences) 2006年第1期26卷 21-24页

作者：骆翔喻志源冯永东王伟 department of neurology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

To examine the effects of ischemia and anoxia on cell activation and cell cycle of astrocytes in vitro, the cell cycles and the proliferation of astrocytes in different time points after ischemia and anoxia were studied by flow cytometry and BrdU labeling and the expression of GFAP and cyclin D1 was detected by the fluorescence immunochemistry. After ischemia and anoxia in vitro, the astrocytes in S phase were significantly increased as compared with those in the normal group and the proliferating ability of the astrocytes was highest 6 h after the treatment as revealed by BrdU pulse labeling, but the astrocytes in S phase and proliferating ability were decreased after 6 h. At the early stages of ischemia and anoxia, the positive staining intensity of GFAP was increased, peaked at 6th h, while 12 h after the ischemia and anoxia, the positive staining intensity of GFAP became weak, and the expression of cyclin D1 was gradually increased after the ischemic and anoxic damage. It is concluded that astrocytes are activated to proliferate and enter new cycle events by ischemia and anoxia, and cyclin D1 is implicated in the proliferation and repair of astrocytes. The cell cycle events are closely associated with the proliferation and activation of astrocytes.

关键词： brain ischemia astrocytes cell cycle

FgBβ链三个基因多态性位点单体型与其功能表达和脑梗死的关联性分析

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现代免疫学 2011年第5期31卷 383-388页

作者：张楠楠元小冬徐建辉邓红亮王淑娟华北煤炭医学院附属开滦医院唐山063000

为分析纤维蛋白原(Fg)Bβ链基因FgB-β1420G/A、-993C/T及BsmAIG/C位点多态性及其单体型对血浆Fg浓度和分子活性等功能表达的影响以及与脑梗死疾病的关系,选取首次新发急性脑梗死(ACI)患者108例,及对照组选取健康志愿者113例,测定了全... 详细信息

为分析纤维蛋白原(Fg)Bβ链基因FgB-β1420G/A、-993C/T及BsmAIG/C位点多态性及其单体型对血浆Fg浓度和分子活性等功能表达的影响以及与脑梗死疾病的关系,选取首次新发急性脑梗死(ACI)患者108例,及对照组选取健康志愿者113例,测定了全部样本的血浆Fg浓度、纤维蛋白单体最大聚合速率(FMPV)、最大光密度值(Amax)、FMPV/Amax,及相关生化指标,并应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和等位基因特异性聚合酶链反应法(AS-PCR)检测各位点等位基因和基因型。结果显示,两组间三个多态性位点等位基因和基因型的频率分布无统计学差异(P>0.05);但ACI组FMPV和Amax明显高于对照组(P2、H3、H6及H7四种主要单体型中,H2、H7在ACI中的分布频率明显高于对照组(P2、H7单体型携带者可以在特定因素作用下导致不同类型的血浆Fg功能表达异常,而引发ACI。

关键词：急性脑梗死纤维蛋白原基因多态性单体型功能表达

Effect of Largehead Atractylodes Rhizome on Serum Albumin Level and Intestinal Mucosa Morphology in Rats with Cerebral Hemorrhage

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医学信息 2010年第6期23卷 1824-1826页

作者： WANG Jian-lin1,ZHANG Su-ming1,YANG Zhi-xiu2,ZHANG Xiao-min2,FAN Yun-hu2,WANG Jing-mei2(1.neurology department,Tongji hospital of Tongji medical college of Huazhong University of Science & Technology Wuhan 430030 2.neurology department of The Second People's hospital of Yunnan province Kunming Yunnan 650021,china) neurology department Tongji Hospital of Tongji Medical College of Huazhong Unversity of Science & Technology Wuhan430030 neurology department of The Second People's hospital of Yunnan province Kunming Yunnan 650021 China

Objective To evaluate the effect and mechanism of largehead atractylodes rhizome on the serum albumin level and jejunal mucosa morphology in rats with cerebral *** The cerebral hemorrhage rat model was established by using a simple grouping experimental design method to detect the serum albumin level and the morphological parameter of the jejunal mucosa in the treatment and control groups on Day 1,7 and 14 after cerebral *** Compared with the control group,the serum albumin was increased in the treatment group on Day 1 and 7 after the operation(P<0.05),the small intestinal villus height and mucosal thickness were significantly decreased on Day 1,and the small intestinal villus height,villus area and mucosal thickness were all significantly increased(P<0.01).Conclusion Largehead atractylodes rhizome can increase the serum albumin level of rats,and can aggravate the injury of the small intestinal mucosa in the early stage,but relieve in the late stage.

关键词： largehead atractylodes rhizome cerebral hemorrhage rat serum albumin intestinal mucosa

煤工尘肺患者血清中白细胞介素-18及其受体水平的变化和意义

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中国职业医学 2009年第3期36卷 196-198,201页

作者：王冬梅袁宝军邹吉敏李超华北煤炭医学院附属开滦医院检验科河北唐山063000

目的探讨血清中白细胞介素-18(IL-18)及其受体(IL-18R)水平在煤工尘肺(CWP)中的变化及意义。方法采用酶联免疫吸附测定法检测134例CWP患者(CWP组)和68例矽肺患者(矽肺组),具有与CWP组相同接尘条件的57例0+矿工(0+组)和64例健康井下接尘... 详细信息

目的探讨血清中白细胞介素-18(IL-18)及其受体(IL-18R)水平在煤工尘肺(CWP)中的变化及意义。方法采用酶联免疫吸附测定法检测134例CWP患者(CWP组)和68例矽肺患者(矽肺组),具有与CWP组相同接尘条件的57例0+矿工(0+组)和64例健康井下接尘矿工(接尘对照组),50例非接尘井上健康人员(正常对照组)血清中IL-18及IL-18R含量,并根据CWP期别及有无合并症、并发症进行分析。结果与正常对照组比较,接尘对照组血清IL-18水平明显降低(P0.05)。CWP组血清IL-18和IL-18R呈正相关(r=0.230,P<0.01),其他各组两者均无相关性。结论IL-18和IL-18R共同参与CWP的发生发展过程,且可能有助于早期CWP病理分型;IL-18R可能预示CWP的严重程度和病情进展。

关键词：煤工尘肺白细胞介素-18 受体意义

尘肺患者全肺灌洗液某些白细胞介素指标检测的意义

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中国职业医学 2007年第6期34卷 472-474页

作者：袁宝军王冬梅邹吉敏李超华北煤炭医学院附属开滦医院检验科河北唐山063000

目的探讨煤矿工人尘肺患者灌洗液中白细胞介素-12p70(IL-12p70)、IL-12p40、IL-18和IL-10水平及意义。方法用酶联免疫吸附双抗体夹心法检测59例尘肺患者(其中煤工尘肺40例、矽肺19例)灌洗液中IL-12p70、IL-12p40、IL-18和IL-10水平。结... 详细信息

目的探讨煤矿工人尘肺患者灌洗液中白细胞介素-12p70(IL-12p70)、IL-12p40、IL-18和IL-10水平及意义。方法用酶联免疫吸附双抗体夹心法检测59例尘肺患者(其中煤工尘肺40例、矽肺19例)灌洗液中IL-12p70、IL-12p40、IL-18和IL-10水平。结果煤工尘肺患者灌洗液IL-18水平随期别增加而明显下降;矽肺患者灌洗液IL-18水平随期别增加而上升,差异均具有显著性(P2p70、IL-12p40和IL-10水平在各组中均无明显改变(P>0.05)。与单纯煤工尘肺比较,煤工尘肺并发肺气肿患者灌洗液IL-10水平下降,差异有显著性(P2p70、IL-12p40和IL-10三者间呈正相关,与IL-18不相关。结论尘肺患者存在异常免疫应答。检测尘肺患者全肺灌洗液IL-18和IL-10水平可能对鉴别尘肺期别及判断疾病严重程度有一定价值。

关键词：尘肺灌洗液白细胞介素

Effect of Stent-Assisted Angioplasty on Serum Levels of Beta-Amyloid in Patients with Intracranial and Extracranial Artery Stenosis

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Effect of Stent-Assisted Angioplasty on Serum Levels of Beta...

6-OHDA Induces Cycle Reentry and Apoptosis of PC12 Cells through Activation of ERK1/2 Signaling Pathway

第六届国际脑血管病高峰论坛

作者： Zhao L,Zhao Y,Zhou Y,Lin S,Jin Q department of neurology,department of medical Examination,Second People’s hospital of Huai’an and affiliated Huai’an hospital of Xuzhou medical college, Huai’an,Jiangsu,china

Objective:The purpose of this study was to investigate the effects of stent-assisted angioplasty on serum levels of beta-amyloids (Aβ) 1-40 and 1-42 in patients with intracranial and extracranial arterial ***:Twenty-four patients with severe intracranial and/or extracranial arterial stenosis received stent-assisted angioplasty in addition to conventional *** 24 patients

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Journal of Huazhong University of Science and Technology(medical Sciences) 2009年第1期29卷 97-100页

作者：张振涛王涛曹学兵孙圣刚王岚 department of neurology Union Hospital Tongji Medical College Huazhong University of Science and Technology

This study investigated the effect and mechanism of cell cycle reentry induced by 6-hydrodopamine （6-OHDA） in PC12 cells. By using neural differentiated PC12 cells treated with 6-OHDA, the apoptosis model of dopaminergic neurons was established. Cell viability was measured by MTT. Cell apoptosis and the distribution of cell cycle were assessed by flow cytometry. Western blot was used to detect the activation of extracellular regulator kinasel/2 （ERK1/2） pathway and the phosphorylation of retinoblastoma protein （RB）. Our results showed that after PC12 cells were treated wtih 6-OHDA, the viability of PC12 cells was declined in a concentration-dependent manner. Flow cytornetry revealed that 6-OHDA could increase the apoptosis ratio of PC12 cells in a time-dependent manner. The percentage of ceils in G0/G1 phase of cell cycle was decreased and that in S phase and G2/M phase increased. Simultaneously, ERK1/2 pathway was activated and phosphorylated RB increased. It was concluded that 6-OHDA could induce cell cycle reentry of dopaminergic neurons through the activation of ERK1/2 pathway and RB phosphorylation. The aberrant cell cycle reentry contributes to the apoptosis of dopaminergic neurons.

关键词： 6-hydrodopamine cell cycle extracellular regulator kinase 1/2 retinoblastorna protein Parkinson' s disease

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外伤闭合性单纯小肠破裂77例临床分析

陕西医学杂志 2007年第6期36卷 686-688页

作者：贺房勇张国志赵鹏陈建立李曙光华北煤炭医学院附属医院普外科唐山063000

目的:探讨外伤闭合性单纯小肠破裂的早期诊断及治疗的方法。方法:回顾分析77例外伤闭合性单纯小肠破裂的临床资料。结果:小肠破裂单纯缝合修补65例,小肠部分切除端端吻合6例,小肠修补加小肠部分切除端端吻合6例,全部治愈。结论:早期诊... 详细信息

目的:探讨外伤闭合性单纯小肠破裂的早期诊断及治疗的方法。方法:回顾分析77例外伤闭合性单纯小肠破裂的临床资料。结果:小肠破裂单纯缝合修补65例,小肠部分切除端端吻合6例,小肠修补加小肠部分切除端端吻合6例,全部治愈。结论:早期诊断依靠外伤史和腹膜炎体征,诊断性腹穿阳性率高,X线检查有气腹征可明确诊断,CT和B超有重要参考价值。及时手术是降低并发症的关键。

关键词：小肠破裂/诊断破裂/外科学

Correlation Study on Expression of GST-π Protein in Brain Tissue and Peripheral Blood of Epilepsy Rats Induced by Pilocarpine

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Journal of Huazhong University of Science and Technology(medical Sciences) 2011年第5期31卷 701-704页

作者：邓学军贾红杨志勇李刚孙圣刚 department of neurology Union Hospital Tongji Medical College Huazhong Science and Technology University

Previous studies have suggested that glutathione-S-transferase π （GST-π） over-expression in the brain tissue is associated with refractory epilepsy. However, whether the change in GST-π level in the peripheral blood is in line with that in brain tissue remains unknown. This study examined the correlation between GST-π in brain tissue and that in peripheral blood in rat models of pilocarpine-induced refractory epilepsy. The animals were divided into drug-resistant group and drug-responsive group according to the response to anti-epileptic drugs. GST-π expression in brain tissue was immunohistochemically determined, while the expression of GST-π in peripheral blood was analyzed by Western blotting. In the hippocampus and cortex, GST-π was mainly found in the cytoplasm and membrane of neurons, and the GST-π expression level was higher in drug-resistant group than in the drug-responsive group and saline control group （P〈0.05）. Moreover, there was no significant difference between responders and saline control animals （P〉0.05）. The change in expression of GST-π in peripheral blood showed the same pattern as that in brain tissues, suggesting GST-π might contribute to drug resistance in epilepsy. Importantly, the GST-π over-expression in peripheral blood could be used as a marker for resistance to anti-epileptic agents.

关键词： GST-π protein epilepsy brain pilocarpine rats correlation study