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Evidence from isotopic geochemistry as an indicator of eutrophication of Meiliang Bay in Lake Taihu, china

Science china Earth Sciences 2006年第z1期49卷 62-71页

作者： LIN Lin, WU Jinglu & WANG Sumin nanjing Institute of Geography & Limnology, Chinese Academy of Sciences, nanjing 210008, china Graduate university of the Chinese Academy of Sciences, Beijing 100039, china Chinese Acad Sci Nanjing Inst Geog & Limnol Nanjing 210008 Peoples R China Chinese Acad Sci Grad Univ Beijing 100039 Peoples R China

In this paper, Lake Taihu, a large shallow freshwater lake in china, is chosen as an example of reconstruction of eutrophication through the comparison between stable isotopes from dissolved nutrients and plants and water column nutrient parameters and integration of multiple proxies in a sediment core from Meiliang Bay including TN, TP, TOC, C/N, δ15N, δ13C, etc. Differences in aquatic plant species and trophic status between East Taihu Bay and Meiliang Bay are indicated by their variations in δ13C and δ15N of aquatic plants and δ15N of NH4+. A significant influence ofexternal nutrient inputs on Meiliang Bay is reflected in temporal changes in δ 15N of NH4+ and hydro-environmental parameters. The synchronous change between δ13C and δ15N values of sedimented organic matter (OM) has been attributed to elevated primary production at the beginning of eutrophication between 1950 and 1990, then recent inverse correlation between them has been caused by the uptake of 15N-enriched inorganic nitrogen by phytoplankton grown under eutrophication and subsequent OM decomposition and denitrification in surface sediments, indicating that the lake has suffered from progressive eutrophication since 1990. Based on the use of a combination of stable isotopes and elemental geochemistry, the eutrophication of Meiliang Bay in Lake Taihu could be better traced. These transitions of the lake eutrophication respectively occurring in the 1950s and 1990s have been suggested as a reflection of growing impacts of human activities, which is coincident with the instrumental data.

关键词： stable carbon isotope of OM, stable nitrogen isotope, eutrophication, Lake Taihu.

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A membrane-tethered transcription factor ANAC089 negatively regulates floral initiation in Arabidopsis thaliana

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Science china(Life Sciences) 2010年第11期53卷 1299-1306页

作者： LI JianQin1,2, ZHANG Juan1, WANG XueChen1 & CHEN Jia1 1 State Key Laboratory of plant Physiology and Biochemistry,college of Biological Sciences, china agricultural university, Beijing 100193, china 2 college of Life Science and Technology, Guangxi university, Nanning 530005, china 1. State Key Laboratory of plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing 100193 China2. College of Life Science and Technology Guangxi University Nanning 530005 China

The plant-specific NAC (NAM, ATAF1/2,and CUC2) transcription factors have a regulatory function in developmental processes and stress responses. Notably a group of NAC members named NTLs (NTM1-Like) are membrane-tethered, ensuring plants rapidly respond to developmental changes and environmental stimuli. Our results indicated that ANAC089 was a membrane-tethered transcription factor and its truncated form was responsible for the physiological function in flowering time control.

关键词： Arabidopsis thaliana ANAC089 floral initiation membrane-tethered transcription factor

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FACULTATIVE MIGRATION OF COTTON BOLLWORM, HELICOVERPA ARMIGERA

FACULTATIVE MIGRATION OF COTTON BOLLWORM, HELICOVERPA ARMIGE...

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The ⅩⅤth International plant protection Congress

作者： ZHANG Xiao-xi Department of Entomology nanjing agricultural university nanjing 210095 china

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Novel molecular Mechanisms Associated with Different Phenotype of Benzimidazole Fungicide Resistance in the Wheat Scab Gibberella zeae

Novel molecular Mechanisms Associated with Different Phenoty...

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中国植物病理学会2005年学术年会暨植物病理学报创刊50周年纪念会

作者： Chang-jun Chen, Hong-xia Li, Li Jun, Jian-xin Wang and Ming-guo Zhou (college of plant protection, nanjing agricultural university, nanjing, 210095)

Three sensitivity phenotypes of wild-type sensitivity, high and moderate resistance were identified among field strains of Gibberella zeae to the benzimidazole fungicide carbendazim (MBC). The whole sequence of the beta-tubulin gene from twenty-eight G. zeae strains representing the three field phenotypes and from five G. zeae laboratory MBC-resistance mutants was separated using PCR. No alternative change was found at the beta-tubulin gene in all MBC-resistant strains both from field and laboratory. Thus, it can be concluded that the mechanism of G. zeae to MBC-resistance is different from other filamentous fungi, which was caused by point mutation at amino acid position 198 or other position of beta-tubulin gene. To look for the carbendazim resistance mechanism of G. zeae, microtubule-associated protein gene (map) and other members of tubulin gene family, i.e. alpha-, alpha2-, gamma-, and no-named tubulins were also cloned and analyzed. The results revealed that there was not any mutation found in alpha-, alpha2-, gamma-tubulins genes and map among three sensitivity phenotypes. However, alternation at codon 33, 64 and 66 in a no-named tubulin gene, which had 997 bp lengths, including one intron, encoding 313 amino acids, corresponded to the different phenotype sensitivities, respectively. At Codon 33, TTT encoding phenylalanine in MBC-sensitive isolates was converted to TAT coding tyrosine in field middle MBC-resistance strains. At codon 64, GAG was altered into CTG resulting in substitution of glutamic acid for leucine in a field high benzimidazole-resistance isolate. While one-nucleotide mutation GAG into AAG also at the same codon 64 resulted in the single amino acid substitution of glutamic acid for lysine in UV induced high MBC-resistance mutants. But at codon 66, only a different single point mutation of TTC (in sensitive isolates) to TAC (in the NT07 isolate) caused a change of phenylalanine (Phe) to a tyrosine (Tyr) expressing middle resistance level, too.

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IAA production of Neotyphodium sincium EHA-1-Roegneria kamoji complex

IAA production of Neotyphodium sincium EHA-1-Roegneria kamoj...

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2010年中国菌物学会学术年会

作者： JI YanLing PAN JieYou WANG ZhiWei~* (college of Life Sciences nanjing agricultural university nanjing 210095 china)

＜正＞Endophytic fungi-grass symbiotum base on either endophyte can synthesize or induce the host plant to produce various kinds of secondary metabolites（De Battista ＆ Bacon 1990）. These secondary metabolites,such as alkaloids and plant hormones usually benefit to plant survival,growth and *** detected IAA concentrations of culture filtrates,cell free macerates of Neotyphodium sincium EHA-1 by ELISA and HPLC,and report here that indole-3-acetic acid（IAA） accumulation in culture *** EHA-1 synthesizing IAA even no tryptophan（Trp）,precursor of IAA was added exogenously into the culture（Ji 2002）. These indicated this isolate can produce plant hormone de *** Trp presence conditions, highest concentration of 47.08 mg/g dry mycelium was recorded in IAA production *** IAA was extracted from aerial tissue of Roegneria kamoji EI and EF plants and analyzed by HPLC,higher concentration of IAA was detected in EI plants comparing with that of EF plants. This phenomenon was recognized on plants in period of heading stage and flowering stage *** suggested that IAA biosynthesis in fungal cells may have a certain contribution in total IAA level within Chinese Roegneria-Neotyphodium symbiotum.

关键词： Neotyphodium sincium EHA-1 Roegneria kamoji IAA HPLC

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AN OBSERVATION STUDY ON BIOLOGICAL CHARACTERS AND MATING BEHAVIOR OF DAHURIAN CASEBEARER(COLEOPHORA DAHURICA FLKV.)ADULTS

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Journal of Northeast Forestry university 1993年第2期4卷 82-86页

作者： Dai Huaguo Song Xiaoling nanjing agricultural university Li Jianlong Northeast Forestry university 1. Dept. of plant protection Nanjing Agricultural University 210014 Nanjing China 2. Northeast Forestry university China

Through rearing tests and observations under laboratory conditions, biological characters and mating behavior of Dahurian casebearer (Coleophora dahurica Flkv.) adults were studied in this paper. Results discovered that the time of emergense of male adults was 24h to 36h earlier than that of female adults. The peak of sex pheromone secretion of the female adults appeared of 24h to 42h after the emergence. The reaction of male adults on the sex pheromone was most sensitive during 30h to 48h after emerged, so the earlier emergence of the male adults was favorable to mating. The phenomenon of repeated mating did not exist in female adults, but the male adults could mate for several times, with maximum of 4 times. There was a definite behavioral reaction during the mating period of male and female adults, and the certain period of feeding and mating times could affect the adults longervity to a certain degree.

关键词： Dahurian casebearer Adults Biological characters Mating behavior

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REGIONALIZATION OF WEED VEGETATION OF ARABLE LAND IN china

REGIONALIZATION OF WEED VEGETATION OF ARABLE LAND IN CHINA

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The ⅩⅤth International plant protection Congress

作者： QIANG Sheng Weed Research Laboratory nanjing agricultural university nanjing 210095 china

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A novel aminated polymeric adsorbent for removing refractory dissolved organic matter from landfill leachate treatment plant

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Journal of Environmental Sciences 2009年第8期21卷 1089-1095页

作者： ZHANG Long, LI Aimin, WANG Jinnan, LU Yufei, ZHOU Youdong State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, nanjing university, nanjing 210093, china. [a]State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Nanjing University Nanjing 210093 China

Refractory dissolved organic matter （DOM） from landfill leachate treatment plant was with high dissolved organic carbon （DOC） content. An aminated polymeric adsorbent NDA-8 with tertiary amino groups and sufficient mesopore was synthesized, which exhibited high adsorption capacity to the DOM （raw water after coagulation）. Resin NDA-8 performed better in the uptake of the DOM than resin DAX-8 and A100. Electrostatic attraction was considered as the decisive interaction between the adsorbent and adsorbate. Special attention was paid to the correlation between porous structure and adsorption capacity. The mesopore of NDA-8 played a crucial role during uptake of the DOM. In general, resin in chloride form performed a higher removal rate of DOC. According to the colunm adsorption test, total adsorption capacity of NDA-8 was calculated to 52.28 mg DOC/mL wet resin. 0.2 mol/L sodium hydroxide solution could regenerate the adsorbent efficiently.

关键词： electrostatic interactions chemically modified resin coagulation adsorption pore structure

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Expression pattern and core region analysis of AtMPK3 promoter in response to environmental stresses

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Science china(Life Sciences) 2010年第11期53卷 1315-1321页

作者： GAO Fei1,2, SU Qi1, FAN YunLiu1& WANG Lei1 1Biotechnology Research Institute/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of agricultural Science, Beijing 100081, china 2college of Life and Environmental Sciences, Minzu university of china, Beijing 100081, china 1. Biotechnology Research Institute/National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Science Beijing 100081 China2. College of Life and Environmental Sciences Minzu University of China Beijing 100081 China

The protein kinase AtMPK3,a component of the MAP kinase cascade,plays an important role in stress signal transduction in plant cells. To clarify how AtMPK3 is regulated at the transcriptional level in response to various environmental factors, the 1016-bp promoter sequence upstream of the transcription start site of the AtMPK3 gene was isolated. Analyses of the promoter sequence using plant promoter databases revealed that the AtMPK3 promoter contains many potential cis-acting elements involved in environmental stress responses. We constructed four deletion mutants of the AtMPK3 promoter, and introduced the intact and truncated promoter sequences fused to the β-glucuronidase (GUS) gene into Arabidopsis. GUS histochemical staining and quantitative fluorometric GUS assays were performed to visualize and compare the expression patterns in response to different environmental stimuli. The region between-188 and-62 upstream of the transcription start site was identified as the essential DNA sequence of the AtMPK3 promoter for responses to drought, high salinity, low temperature, and wounding. These results advance our understanding of the molecular mechanisms controlling AtMPK3 expression in response to different environmental stimuli.

关键词： Arabidopsis thaliana AtMPK3 promoter stress

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Proteome Reference Maps for plant-pathogenic Bacterium Xanthomonas oryzae ***

Proteome Reference Maps for Plant-pathogenic Bacterium Xanth...

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中国植物病理学会2012年学术年会

作者： XU Shu LUO Jian-Ying PAN Xia-Yan LIANG Xiao-Yu CHEN Chang-Jun ZHOU Ming-Guo (college of plant protection,nanjing agricultural university,nanjing 210095,china)

＜正＞The plant-pathogenic bacterium Xanthomonas oryzae *** is the causal agent of bacterial blight,which is one of the most serious diseases of *** reference maps of Xanthomonas oryzae *** were constructed by two-dimensional gel electrophoresis,and 412 spots in the gels representing 302 different protein species were identified by MALDI-TOF-MS/*** identified proteins were assigned to 13 functional categories according to the Kyoto Encyclopedia of Genes and Genomes （KEGG） database and the annotations from the National Center for Biotechnology Information （NCBI） *** were distributed within the following categories:carbohydrate metabolism （15.53%）,amino acid metabolism（13.83%）,lipid metabolism（4.37%）,necleotide metabolism （3.64%）,secondary metabolism（5.1%）,energy metabolism（4.61%）,transportation（15.78%）, replication,transcription and translation（10.68%）,protein folding and degradation（11.65%）,signal transduction（2.43%）,cellular processes（1.94%）,other（6.07%）,and unknown（4.37%）.The data set has been deposited into the World-2DPAGE database（accession no.0044） and the MIAPE （Minimum Information about a Proteomics Experiment ） ? document is accessible at http:// ***/experiment/134/.Annotations of the 412 spots will greatly facilitate further comparative proteomic research of Xanthomonas oryzae ***.

关键词： Reference maps two-dimensional gel electrophoresis plant-pathogenic bacteria Xanthomonas oryzae pv.oryzae

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