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Pedosphere 2003年第3期13卷 219-226页

作者： TANMANZHI ZHANGXUELEI CHENJIE YANWUJIU YANGYUJIAN InstituteofSoilScience theChineseAcademyofSciencesNanjing210008(China) collegeofNationalTerritoryResourceandTourism AnhuiNormalUniversityWuhu241000(China) JinanEnvironmentalprotectionBureau Jinan250012(China)

The diversity of soils derived from different parent materials and developed on different terrains inShandong Province, china, was analyzed with the theory of pedodiversity using a 1:1000000 scale soilsand terrain digital database (SOTER) of Shandong Province in a geographical information system (GIS)environment. Diversity index of soils derived from different parent materials followed the sequence of igneousrock ＞ sedimentary rock ＞ metamorphic rock ＞ unconsolidated material. Diversity index of soils developedon different terrains followed the sequence of plain ＞ medium-gradient mountain ＞ medium-gradient hill＞ valley floor ＞ medium-gradient escarpment zone ＞ depression ＞ high-gradient mountain. Abundancedistributions of these soil groups matched the logarithmic normal distributions.

关键词： abundance distribution pedodiversity shandong province SOTER

Detection of Sensitivity and Resistance Variation of Magnaporthe grisea to Kitazin P, Carbendazim and Tricyclazole

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Rice Science 2004年第Z1期 317-323页

作者： ZHANG Chuan-qing, ZHOU Ming-guo, SHAO Zhen-run, LIANG Gui-mei (Key Laboratory of Monitoring and Management of plant Diseases and Insects, Ministry of Agriculture nanjing agricultural university, nanjing 210095, china National agricultural Technical Extension and Service Center, Ministry of Agriculture, Beijing 100026, china)

One hundred and twenty-nine isolates of Magnaporthe grisea from Guangdong, Guangxi, Anhui and Jiangsu provinces of china were tested for resistance frequency to kitazin P and carbendazim, respectively by the distinctive concentration method. The resistance frequency of the isolates to kitazin P which had not been used in practice for about ten years was as high as 79.1%, and only one carbendazim-resistant isolate was detected in Gaoyao, Guangdong Province (with a frequency of 0.78%). Meanwhile, the minimum inhibition concentration (MIC) of hyphal melanization was adopted to detect the sensitivity of M. grisea to tricyclazole. There existed several different degrees of sensitivity to tricyclazole in the melanin biosynthesis of M. grisea, but no relationship was found between these MIC values completely inhibiting melanization in hyphae and the EC50 values of tricyclazole against rice blast tested in vivo. After the isolates were induced by chemical taming or UV irradiation in laboratory, kitazin P-resistant and carbendazim-resistant mutants were recovered by both the methods, but none of tricyclazole-resistant mutant was obtained.

关键词： Magnaporthe grisea fungicide resistance fungicide mutant kitazin P carbendazim tricyclazole

Root Exudates, Rhizosphere Zn Fractions, and Zn Accumulation of Ryegrass at Different Soil Zn Levels

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Pedosphere 2007年第3期17卷 389-396页

作者： XU Wei-Hong LIU Huai MA Qi-Fu XIONG Zhi-Ting college of Resources and Environmental Sciences Southwest University Chongqing 400716 （China） college of plant protection Southwest University Chongqing 400716 （China） Soil Science and plant Nutrition University of Western Australia Crawley WA 600Y （ Australia） college of Resources and Environmental Sciences Wuhan University Wuhan 430072 （China）

A glasshouse experiment was conducted using a root-bag technique to study the root exudates, rhizosphere Zn fractions, and Zn concentrations and accumulations of two ryegrass cultivars （Lolium perenne L. cvs. Airs and Tede） at different soil Zn levels （0, 2, 4, 8, and 16 mmol kg^-1 soil）. Results indicated that plant growth of the two cultivars was not advérsely affected at soil Zn level ≤ 8 mmol kg^-1. plants accumulated more Zn as soil Zn levels increased, and Zn concentrations of shoots were about 540 μg g^-1 in Aris and 583.9 μg g^-1 in Tede in response to 16 mmol Zn kg^-1 soil. Zn ratios of shoots to roots across the soil Zn levels were higher in Tede than in Airs, corresponding with higher rhizosphere available Zn fractions （exchangeable, bound to manganese oxides, and bound to organic matter） in Airs than in Tede. Low-molecular-weight （LMW） organic acids （oxalic, tartaric, malic, and succinic acids） and amino acids （proline, threonine, glutamic acid, and aspartic acid, etc.） were detected in root exudates, and the concentrations of LMW organic acids and amino acids increased with addition of 4 mmol Zn kg^-1 soil compared with zero Zn addition. Higher rhizosphere concentrations of oxalic acid, glutamic acid, alanine, phenylalanine, leucine, and proline in Tede than in Airs likely resulted in increased Zn uptake from the soil by Tede than by Airs. The results suggested that genotypic differences in Zn accumulations were mainly because of different root exudates and rhizosphere Zn fractions.

关键词： plant Zn accumulation rhizosphere Zn fractions root exudates ryegrass soil Zn level

The optimal immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella and its cross-immunity to Eimeria necatrix and Eimeria acervulina

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The optimal immunization procedure of DNA vaccine pcDNA-TA4-...

Effects of elevated CO_2 on growth and carbon partitioning in rice

中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： Song-Xiaokai,Xu-Lixin,Yan-Ruofeng,Xu-Qianming,Huang-Xinmei,Li-Xiangrui~* (college of Veterinary Medicine,nanjing agricultural university,nanjing 210095,PR china)

Fusion DNAvaccines co-expressed Eimeria tenella TA4 and chicken IL-2(chIL-2) were constructed and their efficacy against *** challenge were *** immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella,including route,dose,time of immunization and age of primary immunization of chicken,was optimized and the stability and the cross-species protection of the vaccine were also *** of immunization was evaluated on the basis of oocyst decrease ratio,lesion score,body-weight gain and the anti-coccidial index(ACI).Results indicated DNA vaccines pcDNA3.1-TA4-IL-2,pcDNA3.1- TA4 and pcDNA4.0c-IL-2 were successfully constructed and the antigen genes could be expressed effectively in *** animal experimental results showed that DNA vaccines could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease *** ACI of pcDNA3.0-TA4-IL-2 group was 192,higher than that of pcDNA3.1-TA4 *** optimization of immunization procedure of DNA vaccine pcDNA-TA4- IL-2 showed that 25μg was the optimal dose and intramuscular injection was the most effective route to induce protective *** were no significant differences of ACIs between boosting and non-boosting *** time and temperature had little effect on the immunizing efficacy of the *** vaccine could provide partial cross-protection against the challenge with *** and ***,but not with E. maxima.

关键词： E.tenella TA4 DNA vaccine chIL-2 immunization procedure cross-protection

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Chinese Science Bulletin 1998年第23期43卷 1982-1986页

作者： LIN Weihong\+1 and WANG Dali\+2 1. Department of plant Nutrition, china agricultural university, Beijing 100094, china 2. Department of Systems Ecology, Research Center for Eco_Environmental Science, Chinese Academy of Sciences, Beijing 100085, china Department of plant Nutrition China Agricultural University Beijing China Department of Systems Ecology Research Center for Eco-Environmental Science Chinese Academy of Sciences Beijing China

Rice (\%Oryza sativa\% cv. Jindao 1187) was grown in open_top chambers which contained ambient and enriched CO\-2. CO\-2 elevation stimulated rice tillering during early vegetative stage. However, panicle dry weight per plant did not change at maturity stage. Root biomass was enhanced by high CO\-2. Root / shoot ratio was increased under high CO\-2 at maturity, indicating more carbon allocation to the below_bround part in rice under high CO\-2.

关键词： CO 2 elevation growth carbon partitioning rice (\%Oryza sativa cv\%. Jindao 1187).

Molecular hydrogen isotope analysis of living and fossil plants—Metasequoia as an example

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Progress in Natural Science:Materials International 2009年第8期19卷 901-912页

作者： Hong Yang a,b, Qin Leng a,c a Department of Science and Technology, college of Arts and Sciences, Bryant university, Smithfield, RI 02917, USA b Department of Geology and Geophysics, Yale university, New Haven, CT 06520, USA c LPS, nanjing Institute of Geology and Palaeontology, CAS, nanjing 210008, china [a]Department of Science and Technology College of Arts and Sciences Bryant University Smithfield RI 02917 USA [b]Department of Geology and Geophysics Yale University New Haven CT 06520 USA [c]LPS Nanjing Institute of Geology and Palaeontology CAS Nanjing 210008 China

Molecular hydrogen isotope analysis preformed on modern and fossil plants has made a significant impact on diverse research fields in biology and geology. Using living and fossil Metasequoia as an example, we review the technology of online GC-IRMS that made the molecular analysis of hydrogen isotope possible and discuss critical issues concerning with the studies of molecular dD and its applications. The apparent hydrogen fractionation factors between lipid molecules and source water (ewater–lipid) vary across plant taxonomy and differ among biomolecules and are affected by multiple environmental factors in which precipitation dD values exercise the first order of control. Eco-physiological factors and environmental parameters are also known to influence dD in plants. Molecular hydrogen isotope analysis of chemically stable lipid molecules, such as n-alkanes, finds a wide range of applications in detecting source sediments, reconstruction of paleoclimatic parameters, inference of air-mass trajectory, as well as in petroleum industry and environmental studies.

关键词： Compound-specific dD plant molecule Hydrogen fractionation Paleoclimate Metasequoia

A hybrid origin to generate Neotyphodium endophytes in some Chinese native grasses

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Research on Indicator System of Regional Eco-efficiency:A Case Study of Chengyang District

2008年中国微生物学会学术年会

作者： Ji Yan-ling~1,Sun Xiang-hui~1,Kang Yan~1,Han Kui~1,Li Wei~2,Yu Han-shou~1,Wang Zhi-wei~1 (~1college of Life Sciences,nanjing agricultural university,nanjing,P.R.china,210095 ~2 Institute of plant protection,Jiangsu Academy of agricultural Sciences,nanjing,210014)

Endophytic fungi are widely surveyed as novel bioresources symbiosis with graminous *** isolates developed mature stromata on culms and inflorescences of Brachypodium sylvaticum grown in Huangshan,Anhui. Morphological characteristics of this endophyte including colony,conidia and phialides,pertherium and asci,host specificity,and phylogenetic characteristics based

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Chinese Journal of Population,Resources and Environment 2006年第4期4卷 54-58页

作者： Zhou Zhenfeng1,2,Sun Lei2,Sun Yinglan2 1.college of Resources&Environment,Laiyang agricultural college,Qingdao,Shandong 266109,china 2.School of Environmental Science and Engineering,Ocean university of china,Qingdao,Shandong 266003,china college of Resources & Environment Laiyang Agricultural College Qingdao Shandong 266109 China School of Environmental Science and Engineering Ocean University of China Qingdao Shandong 266003 China School of Environmental Science and Engineering Ocean University of China Qingdao Shandong 266003 China School of Environmental Science and Engineering Ocean University of China Qingdao Shandong 266003 China

Eco-efficiency is a valuable tool for managing and solving issues involving resource consumption and pollution emission in current production *** the popularity of the term"eco-efficiency"in business,limited attention has been paid to measuring and reporting regional eco-efficiency for local government policy *** on the concept and method of eco-efficiency,an indicator system of regional eco-efficiency is established in this *** indicator system comprises 22 indicators,which are divided in to three categories including socio-economic development,resources consumption, environmental *** a promising statistical technique, principle component analysis is used to set the weight of indicators which attempts to calculate the eco-efficiency indices of Qingdao's Chengyang *** results show that the eco-efficiency of Chengyang District has clearly improved 35.1% with small fluctuation from 1995 to ***-economic development index and resources consumption index also represent obvious increasing *** correlation coefficient between soci-economic development index and resources consumption index is 0.979,which means the social progress and economic growth of Chengyang District depend on an extravagant consumption of *** environmental pressure index increased slowly before 1997 and declined gradually after 1997,due to more attention being paid to environmental protection by local government in recent *** District still keeps the traditional economic development mode with a high consumption and high production,so the emphases of future development should put on improving the improving the efficient use of natural resources and promoting environmental management *** results show that the indicators system of regional eco-efficiency is a promising method to quantitatively evaluate resources and environmental efficiency and provide an effective decision-making support for local governments.

关键词： regional eco-efficiency indicator system Ecoefficiency Synthetic Index (ESI) Principle Component Analysis (PCA)

Use of the modified viral satellite DNA vector to silence mineral nutrition-related genes in plants: silencing of the tomato ferric chelate reductase gene, FRO1, as an example

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Science china(Life Sciences) 2008年第5期51卷 402-409页

作者： HE XiuXia1,2,JIN ChongWei1, LI GuiXin3, YOU GuangYi4, ZHOU XuePing3 & ZHENG ShaoJian4 1 Key Laboratory for Environmental and Ecosystem Health of the Ministry of Education, college of Environmental and Resource Science, Zhejiang university, Hangzhou 310029, china 2 college of Life Science, Changchun university of Science and Technology, Changchun 130022, china 3 Institute of Biotechnology, Zhejiang university, Hangzhou 310029, china 4 State Key Laboratory of plant Physiology and Biochemistry, college of Life Science, Zhejiang university, Hangzhou 310058, china 1. Key Laboratory for Environmental and Ecosystem Health of the Ministry of Education College of Environmental and Resource Science Zhejiang University Hangzhou 310029 China 2. college of Life Science Changchun University of Science and Technology Changchun 130022 China 3. Institute of Biotechnology Zhejiang University Hangzhou 310029 China 4. State Key Laboratory of plant Physiology and Biochemistry College of Life Science Zhejiang University Hangzhou 310058 China

Virus-induced gene silencing (VIGS) is potentially an attractive reverse-genetics tool for studies of plant gene function, but whether it is effective in silencing mineral nutritional-related genes in roots has not been demonstrated. Here we report on an efficient VIGS system that functions in tomato roots using a modified viral satellite DNA (DNAmβ) associated with Tomato yellow leaf curl china virus (TYLCCNV). A cDNA fragment of the ferric chelate reductase gene (FRO1) from tomato was inserted into the DNAmβ vector. Tomato roots agro-inoculated with DNAmβ carrying both a fragment of FRO1 and TYLCCNV used as a helper virus exhibited a significant reduction at the FRO1 mRNA level. As a consequence, ferric chelate reductase activity, as determined by visualization of the pink FeBPDS3 complex was significantly decreased. Our results clearly demonstrated that VIGS system can be employed to investigate gene function associated with plant nutrient uptake in roots.

关键词： virus-induced gene silencing roots ferric chelate reductase