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Effects of Citral on Aspergillus flavus Spores by Quasi-elastic Light Scattering and Multiplex Microanalysis Techniques

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 277-283页

作者： Man LUO1,2, Li-Ke JIANG2, Yao-Xiong HUANG3, Ming XIAO4, Bo LI4, and Guo-Lin ZOU1* 1college of life sciences, Wuhan university, Wuhan 430072, china 2college of life sciences, anhui agricultural university, hefei 230036, china 3 Institute of Biomedical Engineering, Ji’nan university, Guangzhou 510632, china 4Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, the Institute of Biodiversity Science, Fudan university, Shanghai 200433, china college of life sciences Wuhan UniversityWuhan 430072 China college of life sciences Anhui Agricultural UniversityHefei 230036 China Institute of Biomedical Engineering Ji'nan UniversityGuangzhou 510632 China Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering the Institute of Biodiversity Science Fudan UniversityShanghai 200433 China

Citral refined from Litsea cubeba oil has been found to have a strong influence on fungi, especially Aspergillus flavus. Multiplex microanalysis and quasi-elastic light scattering techniques were applied to study the effects of citral on Aspergillus flavus spores from the levels of membrane, organelle and intracellular macromolecule. It was found that citral injured the wall and the membrane of A. flavus spore, resulting in decrease of its elasticity. After entering the cell, citral not only influenced the genetic expression of mitochondrion reduplication and its morphology, but also changed the aggregation of protein-like macromolecules. As a result, cells, organelles and macromolecules lost their normal structures and functions, eventually leading to the loss of germination ability of A. flavus spores. Since Litsea cubeba oil as food additive and antifungal agent is safe and less poisonous, it is important to elucidate the inhibitory mechanisms of Litsea cubeba oil on the germination ability of A. flavus spore.

关键词： citral Aspergillus flavus spore multiplex microanalysis techniques quasi-elastic light scattering technique

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Spatio-temporal variations of the bacterioplankton community composition in Chaohu Lake, china

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Progress in Natural Science:Materials International 2008年第9期18卷 1115-1122页

作者： Chaoling Wei a,, Sumin Bao b, Xinyan Zhu c, Xiangming Huangd a School of Environmental and Resource sciences, anhui agricultural university, 130 West Changjiang Road, hefei 230036, china b School of life sciences, anhui agricultural university, hefei 230036, china c School of Forestry and Landscape Architecture, anhui agricultural university, hefei 230036, china d Chaohu Environmental Protection Monitoring Center, Chaohu 238000, china School of Environmental and Resource sciences Anhui Agricultural University 130 West Changjiang Road Hefei 230036 China School of life sciences Anhui Agricultural University Hefei 230036 China School of Forestry and Landscape Architecture Anhui Agricultural University Hefei 230036 China Chaohu Environmental Protection Monitoring Center Chaohu 238000 China

Despite considerable attention paid to Chaohu Lake in china, the dynamics of bacterioplankton community composition (BCC) on spatial and seasonal scales are poorly *** this study, water samples were collected from autumn 2006 to summer 2007 at five positions in Chaohu Lake with difierent trophic *** of these samples was determined by both the PCR amplification of the 16S rDNA gene and the denaturing gradient gel electrophoresis (DGGE).The abundance and diversity of bacterioplankton communities at difierent sampling positions showed similar seasonal *** BCCs in the samples varied substantially, and the pattern of changes indicated that the seasonal difierence might have a significant impact on the BCC’s structure in the *** correspondence anal-ysis (CCA) on the DGGE patterns and physicochemical parameters indicated that the temperature and the levels of 5-d biochemical O2 demand (BOD5), NH3-N, CODMn, total nitrogen, total phosphorous, and dissolved oxygen significantly infiuenced the BCC, and four of the seven variables were related to the level of *** results indicate that eutrophic status and season are the most infiu-ential factors in determining BCC in Chaohu Lake.

关键词： Chaohu Lake Bacterioplankton community composition Spatial and seasonal dynamics

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ON　THE　UPPER　ESTIMATES　OF　FUNDAMENTAL　SOLUTIONS　OF　PARABOLIC　EQUATIONS　ON　RIEMANNIAN　MANIFOLDS

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Chinese Annals of Mathematics,Series B 1995年第1期16卷 119-130页

作者： LI JIAYU SHAO XIN (Departmellt of Mathematics, anhui university, hefei 230039, china.Basic Department, anhui agricultural college, hefei 230036, china.) anhui UNIV DEPT MATHHEFEI 230039PEOPLES R CHINA anhui AGR COLL DEPT BASICHEFEI 230036PEOPLES R CHINA

The authors first derive the gradient estimates and Harnack inequalties for positive solutions of the equation on(x, t)An(x, t) + b(x, t)' ac(￣, t) + h(x, t)u(x, t) ￣ m ￣ 0on complete Riemannian manifolds, and then derive the upper bounds of any positive L2fundamental solution of the equation when h(x, t) and b(x, t) are independent of t.

关键词： Parabolic equation Gradient estimate Harnack inequality Fundamentalsolution Riemannian manifolds.

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Differentially Expression of Tual,a Tubulin-encoding Gene,during Flowering of Tea Plant Camellia sinensis (L.) *** Using cDNA Amplified Fragment Length Polymorphism Technique

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 653-662页

作者： Wan-Ping FANG~(1,2) Chang-Jun JIANG~1* Mei YU~1 Ai-Hua YE~1 Zhao-Xia WANG~(1,3) 1 Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of Agriculture,anhui agricultural university,hefei 230036,china 2 college Of Horticulture,Nanjing Agriculture university,Nanjing 210095,china 3 Auhui Institute of Education,hefei 230036,china Key Laboratory of Tea Biochemistry and Biotechnology Ministry of Agriculture Anhui Agricultural UniversityHefei 230036 China college Of Horticulture Nanjing Agriculture UniversityNanjing 210095 China Auhui Institute of Educationhefei 230036 China

The complementary DNA （cDNA） amplified fragment length polymorphism technique wasused to isolate transcript-derived fragments corresponding to genes involved in the flowering of tea *** sequence analysis of an approximately 300 bp differential fragment amplified by primer combi-nation E11M11revealed 80%-84% similarity to the corresponding part of an α-tubulin gene of other *** complete cDNA sequence of this α-tubulin was cloned by the rapid amplification of cDNA ends technique;its full length is 1537 bp and contains an open reading frame of 450 amino acid residues with two N-glycosylation sites and four protein kinase C phosphorylation *** deduced amino acid sequences didshow significant homology to the α-tubulin from other plants that has been reported to be a pollen-specificprotein and could be correlated with plant cytoplasm-nucleus-interacted male *** named this completecDNA *** nucleotide and amino acid sequence data of Tual have been recorded in the GenBanksequence database with the accession *** Tual gene was cloned into the pET-32aexpression system and expressed in Escherichia coli BL21trxB（DE3）.The molecular weight of expressedprotein was deduced to be approximately 49 *** blot analysis was used to identify the temporalexpression of Tual in tea *** studies of the effect of Tual protein on pollen tube growth indicatedthe Tual solution obviously promoted the growth of tea pollen tube.

关键词： cDNA-AFLP Camellia sinensis flower bud α-tubulin Western blot pollen

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Characteristics of N Mineralization in Urban Soils of hefei,East china

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Pedosphere 2010年第2期20卷 236-244页

作者： ZHANG Kai,XU Xiao-Niu and WANG Qin School of Forestry and Landscape Architecture,anhui agricultural university,hefei 230036 (china) [a] School of Forestry and Landscape Architecture Anhui Agricultural University Hefei 230036 (China)

Nitrogen cycling has been poorly characterized in urban *** in-situ buried bag incubation technique was used to quantify net rates of N mineralization and nitrification in soils of two urban sites,a street greening belt and a university campus,and a suburban site,a forest park,in hefei,East *** average concentration of extractable NO 3 in the surface soil (0-10 cm) was significantly higher at the urban sites than the suburban park site,whereas extractable NH + 4 concentration was significantly higher at the suburban park site than the urban *** forest park soil had greater potential N mineralization (148.1 μg N cm 3) than the soils from the campus (138.3 μg N cm 3) and street (99.8 μg N cm 3).The net mineralization rates varied between 1.63 and 2.69 μg N cm 3 d 1 and net nitrification rates between 0.82 and 1.02 μg N cm 3 d 1 at the suburban forest park site,but the rates varied from 1.27 to 2.41 μg N cm 3 d 1 and from 1.07 to 1.49 μg N cm 3 d 1,respectively,at the urban campus *** net mineralization and nitrification rates were lower during dry *** from regression analysis indicated that net N mineralization was significantly and positively correlated with soil moisture and soil C/N ratio,and was negatively correlated with soil *** nitrification was,however,significantly and negatively correlated with soil moisture and soil C/N ratio,and was positively correlated with soil *** relative nitrification was 0.763,indicating the dominance of nitrate cycling relative to ammonium cycling at the urban *** urban soils had the great potential for N losses compared to the suburban soils.

关键词： nitrification nitrogen cycling soil properties

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Study on transmittance spectra of ITO thin films by ellipsometric method

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Science china(Technological sciences) 2010年第7期53卷 1893-1896页

作者： SUN ZhaoQi1, CAO ChunBin123, CAI Qi1 & SONG XuePing1 1 School of Physics and Material Science, anhui university, hefei 230039, china 2 School of sciences, anhui agricultural university, hefei 230036, china 3 Key Laboratory of Opto-electronic Information Acquisition and Manipulation, Ministry of Education, hefei 230036, china 1. School of Physics and Material Science Anhui University Hefei 230039 China2. School of Sciences Anhui Agricultural University Hefei 230036 China3. Key Laboratory of Opto-electronic Information Acquisition and Manipulation Ministry of Education Hefei 230036 China

ITO films with thicknesses (134+8) nm, grown on glass substrates by sputtering method, were post-annealed at the temperatures of 100, 200, 300 and 400°C for 1 h, respectively. The as-deposited ITO film was amorphous, but crystallized with annealing at elevated temperatures, as demonstrated by X-ray diffraction. The transmittance spectra of all samples were obtained and subsequently simulated by means of spectroscopic ellipsometry. The optical constants n and k of the films were extracted. With the annealing temperature increasing, the optical constants n and k of the films firstly decreased then increased in the whole investigated wavelength range. The optical band gaps of all films were evaluated and they varied between 3.74 and 3.93 eV.

关键词： ITO films optical constants transmittance spectra spectroscopic ellipsometry analysis

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Studies on Poplar Parthenogenesis Induced by Laser

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Chinese Journal of Lasers 1995年第1期B4卷 91-95页

作者： CHEN Zhengu YU Wansu XU Chenglin LIU Xiaogan (Laser Laboratory,Biotechnoloyg Centre,anhui agricultural university, hefei 230036,china) anhui agricultural Univ Hefei China

ＳｔｕｄｉｅｓｏｎＰｏｐｌａｒＰａｒｔｈｅｎｏｇｅｎｅｓｉｓＩｎｄｕｃｅｄｂｙＬａｓｅｒＳｔｕｄｉｅｓｏｎＰｏｐｌａｒＰａｒｔｈｅｎｏｇｅｎｅｓｉｓＩｎｄｕｃｅｄｂｙＬａｓｅｒ￥ＣＨＥＮＺｈｅｎｇｕ；ＹＵＷａｎｓｕ；... 详细信息

ＳｔｕｄｉｅｓｏｎＰｏｐｌａｒＰａｒｔｈｅｎｏｇｅｎｅｓｉｓＩｎｄｕｃｅｄｂｙＬａｓｅｒＳｔｕｄｉｅｓｏｎＰｏｐｌａｒＰａｒｔｈｅｎｏｇｅｎｅｓｉｓＩｎｄｕｃｅｄｂｙＬａｓｅｒ￥ＣＨＥＮＺｈｅｎｇｕ；ＹＵＷａｎｓｕ；ＸＵＣｈｅｎｇｌｉｎ；ＬＩＵＸｉａ...

关键词： poplar ovary callus rate differetiation rate aberration frequence

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Purification of a Novel Antibacterial Short Peptide in Earthworm Eisenia foetida

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 297-302页

作者： Yan-Qin LIU1,2, Zhen-Jun SUN1*, Chong WANG1, Shi-Jie LI2, and Yu-Zhi LIU2 college of Resource and Environment Science, china agricultural university, Beijing 100094, china 2college of Animal Science, Hebei agricultural university, Baoding 071001, china college of Resource and Environment Science China Agricultural UniversityBeijing 100094 China college of Animal Science Hebei Agricultural UniversityBaoding 071001 China

A novel antimicrobial short peptide was purified from earthworm (Eisenia foetida) by a five-step protocol including ammonium sulfate precipitation, ultrafiltration, DE-52 ion exchange chromatography, Sephadex G-10 column chromatography, and C-18 reversed-phase HPLC techniques. The purified peptide was applied to the MALDI-TOF MS to determine the molecular mass and was also subjected to TOF MS-MS analysis to determine the amino acid sequence. As a result, a novel antibacterial peptide, named OEP3121, was obtained, with the molecular mass of 510.8 Da and the sequence being “ACSAG”.

关键词： antibacterial peptide earthworm purification

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Expression pattern and core region analysis of AtMPK3 promoter in response to environmental stresses

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Science china(life sciences) 2010年第11期53卷 1315-1321页

作者： GAO Fei1,2, SU Qi1, FAN YunLiu1& WANG Lei1 1Biotechnology Research Institute/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of agricultural Science, Beijing 100081, china 2college of life and Environmental sciences, Minzu university of china, Beijing 100081, china 1. Biotechnology Research Institute/National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Science Beijing 100081 China2. College of Life and Environmental Sciences Minzu University of China Beijing 100081 China

The protein kinase AtMPK3,a component of the MAP kinase cascade,plays an important role in stress signal transduction in plant cells. To clarify how AtMPK3 is regulated at the transcriptional level in response to various environmental factors, the 1016-bp promoter sequence upstream of the transcription start site of the AtMPK3 gene was isolated. Analyses of the promoter sequence using plant promoter databases revealed that the AtMPK3 promoter contains many potential cis-acting elements involved in environmental stress responses. We constructed four deletion mutants of the AtMPK3 promoter, and introduced the intact and truncated promoter sequences fused to the β-glucuronidase (GUS) gene into Arabidopsis. GUS histochemical staining and quantitative fluorometric GUS assays were performed to visualize and compare the expression patterns in response to different environmental stimuli. The region between-188 and-62 upstream of the transcription start site was identified as the essential DNA sequence of the AtMPK3 promoter for responses to drought, high salinity, low temperature, and wounding. These results advance our understanding of the molecular mechanisms controlling AtMPK3 expression in response to different environmental stimuli.

关键词： Arabidopsis thaliana AtMPK3 promoter stress

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Microstructure and optical properties of nano Ag-ITO films

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Science china(Technological sciences) 2010年第5期53卷 1266-1270页

作者： XIAO Lei1,3,CAO ChunBin2,3,SONG XuePing1,3,MENG FanMing1,3 & SUN ZhaoQi1,3 1 School of Physics and Material Science,anhui university,hefei 230039,china 2 School of sciences,anhui agricultural university,hefei 230036,china 3 Key Laboratory of Opto-Electronic Information Acquisition and Manipulation,Ministry of Education,hefei 230039,china 1. School of Physics and Material Science Anhui University Hefei 230039 China3. Key Laboratory of Opto-Electronic Information Acquisition and Manipulation Ministry of Education Hefei 230039 China2. School of Sciences Anhui Agricultural University Hefei 230036 China

Nano Ag-ITO films with Ag volume fraction of 0.3%-1.0% were prepared by radio-frequency magnetron co-sputtering and analyzed by X-ray diffraction,scanning electron microscopy and ultraviolet-visible *** analysis shows that the films are composed of polycrystalline ITO matrix embedded with Ag nanoparticles with a mean size of 60-100 *** spectra of Ag-ITO films indicate that the visible light transmissivity of the films decreases with increasing the Ag *** transmissivity of the annealed films is higher than that of the as-deposited *** volume 0.3% Ag-ITO films have the highest light *** annealed films exhibit lower light absorptance than as-deposited films.A surface plasmon resonance(SPR) peak of volume 0.3% Ag-ITO films is located around 510 *** with the annealed ITO film,the annealed volume 0.3% Ag-ITO film shows 10% higher reflectivity,while its transmittance is almost the same as that of the annealed ITO film,indicating its potential application in new-type transflective displays.

关键词： nanoparticle films microstructure optical properties transflective displays

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