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Science china {1. 2006年第5期49卷 407-410页

作者： CHENG Mingming1.SUN Zhenying1.2,MA Wanhong1.CHEN Chuncheng1.ZHAO Jincai1.& WANG Zhengping2 1. Laboratory of Photochemistry,{1. for Molecular Science,institute of chemistry,Chinese Academy of Sciences,beijing 1.0080,china 2. Department of Chemical Engineering,Harbin Engineering University,Harbin 1.0001.china 1. Laboratory of Photochemistry Center for Molecular Science Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China 2. Department of Chemical Engineering Harbin Engineering University Harbin 150001 China

The organic-inorganic layered solid catalyst Fe(bpy)_3^(2+)-laponite was ableto photode-grade Rhodamine B (RhB) by activation of dioxygen under visible irradiation (λ > 420nm), while the homogeneous aqueous solution Fe(bpy)_3^(2+) showed no photoactivity. The catalystcould be repeatedly used and retained its reactivity. The hybrid catalyst could be separated fromthe suspension by centrifugation or sedimentation. The TOC removal yield of RhB was *** oxygen species (ROS) formed during degradation was detected by EPR. A possible mechanismwas proposed on the basis of the experimental results.

关键词： laponite, Rhodamine B, molecular oxygen, visible light.

Modeling the intracellular dynamics for Vif-APO mediated HIV-1.virus infection

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Chinese Science Bulletin 2010年第22期55卷 2329-2340页

作者： WANG Yi1.2 & LAI LuHua1.3 1.center for Theoretical Biology, Peking University, {1. 1.0871. china 2 School of Physics, Peking University, {1. 1.0871. china 3 BNLMS, State Key Laboratory for Structural chemistry of Unstable and Stable Species, College of chemistry and Molecular Engineering, Peking University, {1. 1.0871. china center for Theoretical Biology Peking University Beijing 100871 China School of Physics Peking University Beijing 100871 China BNLMS State Key Laboratory for Structural Chemistry of Unstable and Stable Species College of Chemistry and Molecular Engineering Peking University Beijing 100871 China

The viral infectivity factor (Vif) was found to be essential for controlling HIV-1.virus infectivity. It targets cellular antiviral proteins in APOBEC family (APO) to trigger its fast degradation and inhibits APO packaging into nascent virion. In the present study, we propose a mathematical model to quantitatively study the intracellular dynamics of these typical virus-host interactions. Four sets of published experimental data were compared with simulation results to justify the model. Systematic parameter sensitivity and perturbation analysis showed that parameters related to APO are crucial to the infectivity of newly synthesized HIV-1.virus. Interestingly, we discovered that the synthesis rate of the viral structure protein Gag and the required number per nascent virion are optimized to achieve high virion production with minimal level of packaged APO, and large portion of model parameters are beneficial to virus only within a relatively small range. Furthermore, minor variations in several parameters related to viral protein Tat, the activator of viral RNA synthesis, were found to induce switch-like behaviors on both incorporated Vif and APO. These findings may provide new insights for understanding the high mutation rate of HIV-1.virus and its latency, as well as help identify key targets for therapeutic design.

关键词：病毒感染载脂蛋白 HIV 细胞内动力学抗病毒蛋白建模介导

Study of the effect of atorvastatin on the interaction between ICAM-1.and CD1.b by live-cell single-molecule force spectroscopy

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Science china {1. 2010年第4期53卷 752-758页

作者： LI Yi1. SHI XiaoLi2, LIU HuiLiang1. YI ShaoQiong3, ZHANG XueJie2 & FANG XiaoHong2 1.epartment of Cardiology, the General Hospital of Chinese People’s Armed Police Forces, beijing 1.0039,{1. 2beijing National Laboratory for Molecular Sciences institute of chemistry, Chinese Academy of Sciences, beijing 1.01.0,{1. 3State Key Laboratory of Pathogen and Biosecurity beijing institute of Microbiology and Epidemiology, beijing 1.0071.{1. 1. Department of Cardiology the General Hospital of Chinese People’s Armed Police Forces Beijing 100039 China2. Beijing National Laboratory for Molecular Sciences Chinese Academy of Sciences Beijing 100190 China4. Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China3. State Key Laboratory of Pathogen and Biosecurity Beijing 100071 China5. Beijing Institute of Microbiology and Epidemiology Beijing 100071 China

The interaction between the cell adhesion molecule CD1.b and its ligand ICAM-1.plays an important role in inflammatory responses in the disease of atherosclerosis. Atorvastatin is a commonly prescribed statin drug which has been considered as one of the most potent therapeutic agents for atherosclerosis due to its lipid-lowering effect. Recently, there is a growing body of evidence that atorvastatin has anti-inflammatory effect. We have applied the advanced method of live-cell single-molecule force spectroscopy to investigate the effect of atorvastatin on adhesion force between ICAM-1.and CD1.b. Our result showed that single-molecule binding force of ICAM-1.and CD1.b detected by AFM in the living cells was about 40 pN, and atorvastatin did not affect this force by blocking ICAM-1.or CD1.b. This was different from the ICAM-1.monoclonal antibody, which could directly reduce the binding force of ICAM-1.and CD1.b. Flow cytometry results revealed that atorvastatin pretreatment decreased the ICAM-1.expression in TNF-α activated HUVECs, which may contribute to its anti-inflammatory effect. The study provides a new approach to study anti-inflammatory mechanism for clinic drugs.

关键词： atherosclerosis atorvastatin atomic force microscopy single-molecule force spectroscopy protein-protein interaction

Novel gaseous transient species:generation and characterization

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Science china {1. 2007年第2期50卷 145-169页

作者： ZENG XiaoQing1.2 & WANG DianXun1. State Key Laboratory for Structural chemistry of Unstable and Stable Species,{1. of chemistry,Chinese Academy of Sci-ences,beijing 1.0080,china 2 Graduate School of Chinese Academy of Sciences,beijing 1.0039,china 1. State Key Laboratory for Structural chemistry of Unstable and Stable Species Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China 2. Graduate School of Chinese Academy of Sciences Beijing 100039 China

Due to special properties of transient species, such as short-lived, unstable, reactive, and even explosive, the generation and subsequent characterization is a great challenge for experimental chemists. In our laboratory, systematic researches have been carried out to investigate novel transient species: reactive halogen species (RHS), short-lived free radicals, and metastable pseudohalogen compounds, based on the successive technical improvements on the Hel Photoelectron spectroscopy (PES). In this review, the topic mainly focuses on innovative methods of generating novel transient species, and subsequent geometric and electronic structure characterizations of these species combined with quantum chemical calculations.

关键词： reactive halogen species radicals pseudohalogen compounds Hel Photoelectron Spectroscopy (PES) quantum chemical calculations

Acid/alkali gated photochromism of two diarylperfluorocyclopentenes

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Science china {1. 2007年第5期50卷 581-586页

作者： CHEN ZiHui1. ZHAO ShengMin2, LI ZhongYu1. ZHANG Zhi1.& ZHANG FuShi1.1.The Key Lab of Organic Optoelectronics & Molecular Engineering of Ministry of Education, Department of chemistry, Tsinghua University, {1. 1.0084, china 2 Lab of Printing & Packing Materials and Technology-{1. Area Major Laboratory, {1. institute of Graphic Communication, {1. 1.0084, china 1. The Key Lab of Organic Optoelectronics & Molecular Engineering of Ministry of Education Department of Chemistry Tsinghua University Beijing 100084 China 2. Lab of Printing & Packing Materials and Technology-beijing Area Major Laboratory Beijing Institute of Graphic Communication Beijing 100084 China

Two novel diarylfuorocyclopentenes, which underwent gated photochromism, were synthesized. It was found that the photochromic property of these diarylethenes could be switched by alternative addition of acids/alkalis,... 详细信息

关键词： photochromism, diarylethene, gated reactivity, pyridinium betaine

FTIR Spectroscopic Study of Crystallization of Uniaxial Drawn Syndiotactic Polystyrene/Carbon Nanotube Nanocomposite Films

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Chemical Research in Chinese Universities 2012年第1期28卷 153-155页

作者： ZHANG Jin-hao1.2, YANG Ji-ping1. YUAN Cui-ping1.2 and CHEN Guang-ming2 1. School of Materials Science and Engineering, Beihang University, beijing 1.01.1. P. R.{1. 2. beijing National Laboratory for Molecular Sciences(BNLMS), institute of chemistry, Chinese Academy of Sciences, beijing 1.01.0, P. R.{1. Beihang Univ Sch Mat Sci & Engn Beijing 100191 Peoples R China Chinese Acad Sci Inst Chem BNLMS Beijing 100190 Peoples R China

The crystallization of uniaxial hot drawn syndiotactic polystyrene/multi-walled carbon nanotube （sPS/MWCNT） nanocomposite films was studied by FTIR spectroscopy. The effects of MWCNT content, draw ratio and drawing temperature on the sPS crystallinity were investigated. The sPS/MWCMT nanocomposite films show reduced crystallinities with the increase of MWCNT content. In addition, with the increase of draw ratio, both the pure sPS and the sPS/MWCNT nanocomposite drawn films exhibit increased crystallinity. The effect of drawing temperature on the sPS crystallization is complex. In a temperature range of 1.0―1.5 °C, the crystallinity decreases with drawing temperature, whereas it increases at 1.0 °C for both pure sPS and its nanocomposite films.

关键词： Carbon nanotube Crystallization FTIR spectroscopy Nanocomposite Syndiotactic polystyrene

Adsorption behavior of hexadecyltrimethylammonium bromide (CTAB) to mica substrates as observed by atomic force microscopy

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Science china {1. 2005年第2期48卷 101-106页

作者： ZHAO Feng1.2, DU Yukou1. YANG Ping1. LI Xingchang2 & TANG Ji’an2 1. chemistry and Chemical Engineering Department, Suzhou University, Suzhou 21.006,{1. 2. Key Laboratory of Colloid and Interface Science, institute of chemistry, Chinese Academy of Sciences, beijing 1.0080,{1. Correspondence should be addressed to Tang Ji’an (email: jatang@***) 1. chemistry and Chemical Engineering Department Suzhou University 215006 Suzhou China 2. Key Laboratory of Colloid and Interface Science Institute of Chemistry Chinese Academy of Sciences 100080 Beijing China

Atomic force microscopy is employed to study the adsorption behavior of CTAB to mica surfaces. Results show that conformational transitions from globular micelles→cylindrical micelles→flat films are observed in the process of the adsorption of CTAB to mica at twice the bulk critical concentration. However, in 0.5 cmc CTAB solution, the adsorbed CTAB molecules to mica form the layered film structures, and apparent conformational transitions do not appear. Meanwhile, the phenomena of the adsorption of CTAB to mica and the dissociation of mica sur- faces are periodically observed.

关键词： atomic force microscopy (AFM), micelle, behavior of adsorption, the dissociation of mica surfaces.

N-glycosylation proteome of endoplasmic reticulum in mouse liver by ConA affinity chromatography coupled with LTQ-FT mass {1.

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Science china {1. 2010年第4期53卷 768-777页

作者： SONG LiNa1. WANG JingLan1. LIU JinFeng1. LU Zhuang1.3, SUI ShaoHui1. JIA Wei1., YANG Bing1. CHI Hao2, WANG LeHeng2, HE SiMin2, YU WenFeng1. MENG LingYan1. CHEN Shuo1. PENG Xu1. LIANG YiMin1. CAI Yun1.& QIAN XiaoHong1.1.tate Key Laboratory of Proteomics, beijing Proteome Research {1., beijing institute of Radiation Medicine, beijing 1.2206, china 2institute of Computing Technology, Chinese Academy of Sciences, beijing 1.01.0, china 3beijing institute of Biotechnology, beijing 1.0081. china 1. State Key Laboratory of Proteomics Beijing Proteome Research Center Beijing Institute of Radiation Medicine Beijing 102206 China3. Beijing Institute of Biotechnology Beijing 100081 China2. Institute of Computing Technology Chinese Academy of Sciences Beijing 100190 China

Glycosylation is the most versatile and one of the most significant protein post-translational modifications. It is generally classified into three categories according to the amino acid to which the glycan is attached: N-glycosylation, O-glycosylation and C-glycosylation. Synthesis of N-glycoproteins occurs in the rough endoplasmic reticulum (rER), and all N-glycoproteins synthesized in rER have uniform glycan endings with mannose (Man) and glucose (Glc). A systematic strategy was developed to comprehensively profile N-glycoproteins in complex biological samples. The lectin Concanvalin A (ConA), which has a high affinity for glycans ending with Man, was used to extract the N-glycoproteins synthesized or located in the rER, and identified the N-glycoproteins and their glycosylation sites by LTQ-FT MS. The analysis was repeated three times at both the biological sample and mass {1. levels. In total, 323 glycosylation sites on 21. N-glycoproteins were identified in the mouse liver. Of these, 1.1.were known N-glycoproteins in the Swissprot database. The identified N-glycoproteins were classified according to their cell location by the Swissprot database and GO software. The identified N-glycoproteins in this study would serve as a good complement to the N-glycoprotein database for the mouse liver.

关键词： glycosylation rER lectin affinity chromatography ConA LTQ-FT MS

Surface effects of monolayer-protected gold nanoparticles on the redox reactions between ferricyanide and thiosulfate

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Science china {1. 2005年第5期48卷 424-430页

作者： LI Di1. SUN Chunyan1. HUANG Yunjie1. LI Jinghong1.2 & CHEN Shaowei3 1. State Key Laboratory of Electroanalytical chemistry, Changchun {1. of Applied chemistry, Chinese Academy of Sciences, Changchun 1.0022, china 2. Department of chemistry, Tsinghua University, beijing 1.0084, china 3. Department of chemistry, University of California, Santa Cruz, Santa Cruz, CA 95064, USA 1. State Key Laboratory of Electroanalytical chemistry Changchun {1. of Applied chemistry Chinese Academy of Sciences 130022 Changchun China 2. Department of chemistry Tsinghua University 100084 Beijing China 3. Department of chemistry University of California 95064 Santa Cruz Santa Cruz CA USA

Electron transfer through the self-assembled monolayers (SAMs) on gold nanopar-ticles is investigated by using the monolayer protected gold nanoclusters (MPCs) as elec-tron-transfer mediators. 3-Mercaptopropionic acid (MPA) and 1.-meraptoundecanoic acid (MUA) MPCs were employed to catalyze the redox reaction between potassium ferricyanide and sodium thiosulfate. The catalytic mechanism was proposed that the MPCs act as diffusing elec-tron-mediators and electron transfers to and from the MPCs surface. Therefore the electron transfer rate through the capping layers would be proportional to the MPCs catalyzed reaction rate, which was monitored by the UV absorbance of ferricyanide. The calculated apparent rate constant was orders of magnitude smaller than that of the maximum of tunneling current, which was attributed to the splited energy level of the nanoscale particles.

关键词： electron transfer, three-dimensional self-assembled monolayers, redox reaction, electron transfer mediator, surface effect.

Study on the two and three-parameter working stability regions of 3Y-TZP suspension

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Science china(Technological Sciences) 2006年第5期49卷 547-568页

作者： CHEN Youqiang1. 2, ZHANG Xinni3, CHEN Sha’ou3 & QI Ping3 1. Department of chemistry, Qingdao University, Qingdao 266071.{1. 2. Department of chemistry, Tsinghua University, beijing 1.0084,{1. 3. Department of Physics, Qingdao University, Qingdao 266071.{1. 1. Department of chemistry Qingdao University Qingdao 266071 China 2. Department of chemistry Tsinghua University Beijing 100084 China 3. Department of Physics Qingdao University Qingdao 266071 China

We determined the variation tendency of viscosity (η) at various solids load- ing (α) for 3Y-TZP suspension as a function of dispersant concentration (Cw) using vis- cosity measurement, measured the diameter of particles as a function of Cw in very dilute suspension using light scattering method, and obtained the surface features of the sedi- ment of suspension with different Cw using SEM. We also discussed the influence of the microstructure of adsorbed polymer layers on particles and their interactions in the dis- persing medium on the stability of suspension. Then two different stable states and two different unstable states for 3Y-TZP suspension were given. Accordingly, the (Cw, η), (Cw, α), and (α, η) two-parameter, and (Cw, α, η) three-parameter stability maps were con- structed. Based on the DLVO theory calculations, the dispersant concentration, particle distance (r), and interparticle potential energy (VT) maps with various solids loading for 3Y-TZP aqueous suspension were also constructed. In (Cw, r) two-parameter coordinate system, the stable motion region map for particles was established. The Cw, α, and VT,max (the highest potential energy) surface of the potential barrier map was obtained through calculation and simulation on the basis of the surfaces of Cw, r, VT maps introduced above. The (Cw, α) two-parameter stability map was then obtained from the Cw, α, VT,max map. The results showed that this theoretical map can qualitatively prove the experimentally obtained results—the existence of different dispersed states of particles in suspension system and the variation tendency of suspension stability with changing each parame- ter—were reasonable.

关键词： 3Y-TZP, suspension, stability, DLVO theory, interparticle potential energy.