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Expression and function analysis of the metallo-thionein-like (MT-like) gene from Festuca rubra in Chlamydomonas reinhardtii chloroplast

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Science China(Life Sciences) 2008年第12期51卷 1076-1081页

作者： HAN SiHai1,2, HU ZhangLi1 & LEI AnPing1 1 Institute of Eco-Environment, College of Life Science, Shenzhen University, Shenzhen 518060, China 2 Research Center for Analytical Instrumentation, state key lab of Industrial Control technology, Institute of Cyber-systems and Control, Zhejiang University, Hangzhou 310058, China Institute of Eco-Environment College of Life Science Shenzhen University Shenzhen China Research Center for Analytical Instrumentation State Key Lab of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Hangzhou China

The cDNA of the metallothionein-like (MT-like) gene from Festuca rubra cv. Merlin was optimized with bias codon of Chlamydomonous reinhardtii chloroplast genome. The optimized MT-like gene was de-livered into C. reinhardtii chloroplast and the transgenic strains expressing MT-like gene was obtained. PCR-Southern blot and RT-PCR-Southern blot analysis demonstrated that the MT-like gene was inte-grated into chloroplast genome of C. reinhardtii and expressed at the transcriptional level. The cad-mium binding capacity of the transgenic C. reinhardtii was determined by hydride generation-atomic fluorescence spectrometry (HG-AFS) and the binding properties were analyzed. Results showed that the transgenic C. reinhardtii expressing the MT-like gene exhibited remarkably higher Cd2+ binding capacity and grew to higher densities at toxic Cd2+ concentrations (40-100 μmol/L) than the wild type strain, and that the IC50 of Cd2+ (3-d treating ) to algal cell growth of transgenic strain was 55.43% higher than that of the wild type strain, indicating that the Cd2+ binding capacity and Cd2+ tolerance of C. reinhardtii was enhanced through the expression of the foreign MT-like gene in chloroplast.

关键词： Chlamydomonas reinhardtii chloroplast metallothionein-like (MT-like) gene cadmium binding properties

A new one-bit diFFerence collision attack on HAVAL-128

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Science China(Information Sciences) 2012年第11期55卷 2521-2529页

作者： ZHANG WenYing1,2,3,LI YanYan1 & WU Lei1 1School of Information Science and Engineering,Shandong Normal University,Jinan 250014,China 2state key lab of Information Security,Institute of Information Engineering,Chinese Academy of Sciences,Beijing 100093,China 3Shandong Provincial key laboratory for Novel Distributed Computer Software technology,Jinan 250014,China School of Information Science and Engineering Shandong Normal University Jinan China state key lab of Information Security Institute of Information Engineering Chinese Academy of Sciences Beijing China Shandong Provincial key laboratory for Novel Distributed Computer Software technology Jinan China

In this paper,we give a new fast attack on *** attack includes many present methods of constructing hash ***,we present a neighborhood *** propose a new difference path different from the previous *** conclusion is that,when the output of each step satisfies our condition,the message m can collide with m'= m + Δm,where Δm =(0,0,0,0,231,0,...,0).There is only one bit difference between m and m'.Two pairs of collision examples for HAVAL-128 are *** order to improve the probability of collision,we use four tricks of message *** attack's running time is less than 225.83 2-pass HAVAL computations,which is the best result for one-bit collision of HAVAL so far.

关键词： cryptography hash function HAVAL-128 collision message modification

Surface Microstructure Characterization of Sol-gel Derived Porous TiO_2 Thin Films

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Rare Metals 2001年第3期20卷 157-163页

作者： Jiaguo Yu, Jimmy C. Yu, Bei Cheng, A. S. K. Li, Pak Kan Tse 1) Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, China 2) state key lab of Advanced technology for Materials Synthesis and Processing, Wuhan University of technology, W 香港中文大学香港武汉工业大学材料复合新技术国家重点实验室湖北武汉 430070

Porous TiO2 thin films were prepared from alkoxide solutions with and without polyethylene glycol (PEG) by sol-get route on soda lime glass, and were characterized by atomic force microscopy (AFM), transmission electron microscopy (TEM) and X-ray diffraction (XRD). The results show that TiO2 film prepared from precursor solution without PEG is composed of spherical particles of about 100 nm and several nanometer mesoporous pores. With the increase of the amount of PEG added to the precursor solution, the diameter and the depth of the pores in the resultant films increas on the decomposition of PEG during heat-treatment, which lead to them increase of the surface roughness of the films. XRD and TEM results show that the single anatase phase is precipitated and there are some orientation effects in (101) direction.

关键词： sol-gel route porous TiO2 thin films surface microstructure characterization

Controlling Growth of ZnO Nanostructures Via A Solution Route

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Journal of Wuhan University of technology(Materials Science) 2009年第2期24卷 249-253页

作者：贾铁昆 state key lab of Advanced technology for Material Synthesis and Processing Wuhan University of technology

A solution method was developed for fabricating ZnO nanostructures using （NH4）2CO3 as starting material SEM analysis shows that ZnO nanostructures exhibit nanorod, branch and flower-like morphologies. The crystal phase of as-synthesized products was characterized by X-ray diffraction （XRD）. The growth process, formation mechanism and optical property were also discussed by means of transmission electronic microscopy （TEM）, high resolution transmission microscopy （HRTEM） and photoluminescence （PL）. The growth direction of ZnO nanostructures was investigated based on the results of HRTEM. The PL spectrum shows two strong peaks （centered at around -387 and -470 nm） and a broad Deak （centered at around -580 nm）.

关键词： ZnO nanostructures pH solvent luminescence property

Research on user-aware QoS based Web services composition

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The Journal of China Universities of Posts and Telecommunications 2009年第5期16卷 125-130页

作者： LIU Feng , LEI Zhen-ming key laboratory of Information Processing and intelligent technology, Beijing University of Posts and Telecommunications, Beijing 100876, China key laboratory of Information Processing and intelligent technology Beijing University of Posts and Telecommunications Beijing 100876 China

To improve user experience of composite Web services, a user-aware quality of service （QoS） based Web services composition model is proposed. Under such model, a Web services selection method based on quantum genetic algorithm is proposed. This algorithm uses quantum bit encoding, dynamic step-length quantum gate angle adjustment, neighborhood service search and dynamic punishment strategy to expand search scope and speed up convergence. Simulation experiment shows that this algorithm is more efficient than other existing algorithms in Web services selection.

关键词： Web services composition user awareness quality of service quantum genetic algorithm

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带有资格迹的模糊CMAC控制仿真研究

系统仿真学报 2004年第11期16卷 2604-2607页

作者：沈智鹏郭晨大连海事大学航海动态仿真与控制重点实验室大连116026

提出一种带有资格迹的模糊CMAC控制器(FCE),资格迹的引入可以对控制系统进行超前预测,提高系统的稳定性。研究了FCE系统的构成,并推导其学习算法。为适用于在线控制,给出了高效的FCE算法实现方案。应用于船舶航向控制的仿真结果表明,当... 详细信息

提出一种带有资格迹的模糊CMAC控制器(FCE),资格迹的引入可以对控制系统进行超前预测,提高系统的稳定性。研究了FCE系统的构成,并推导其学习算法。为适用于在线控制,给出了高效的FCE算法实现方案。应用于船舶航向控制的仿真结果表明,当存在风浪干扰海况下,船舶航向的控制仍能取得令人满意的效果。

关键词：资格迹模糊CMAC FCE控制器船舶航向控制

Preparation of TiAl/Ti_2AlC Composites with Ti/Al/C Powders by In-situ Hot Pressing

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Journal of Wuhan University of technology(Materials Science) 2006年第2期21卷 14-16页

作者：梅炳初严明 state key lab of Advanced technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 China

TiAl/Ti2AlC composites were prepared by in-situ hot pressing of TilAl/C powders mixtures and sintered at different temperatures were investigated by X- ray diffraction （ XRD ） of samples. The reaction procedure of Ti-Al-C system could be divided into three stnges. Below 900℃ , Ti reacts with Al to form TiAl intermetallics ; above 900 ℃ , C reacts with remain Ti to form TiC triggered by the exothermal reaction of Ti and Al ; TiAl reacts with TiC to produce dense TiAl/Ti2AlC *** the holding stage, ternary Ti2AlC develops to layered polycrystal and composites pyknosis in the meanwhile. The mechanism of synthesis and microstructure was especially discussed.

关键词： Ti-Al-C system TiAl/Ti2AlC composites in-situ hot pressing microstructure

Facile Synthesis of Uniform Hollow Hematite Sub-micro Spheres with Controllable Shell Thickness

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Journal of Wuhan University of technology(Materials Science) 2010年第1期25卷 32-38页

作者：闫共芹官建国 state key lab of Advanced technology for Materials Synthesis and Processing Wuhan University of Technology

A simple method was developed to prepare the uniform hematite hollow submicro-spheres with controllable structure and different diameter based on monodisperse poly（styrene-co-acrylic acid） [P（St-co-AA）] particles. The structure and formation mechanism of the hollow spheres were investigated in detail. The control mechanism of shell thickness was also discussed. The results indicated that the shell thickness and coarseness of the synthesized core-shell hematite hollow spheres could be tuned simply by the surface carboxyl content of the P（St-co-AA） particles. This method provided a new approach for the structure control in the preparation of hollow spheres. A Brunauer-Emmett-Teller （BET） test shows that the prepared hollow spheres have large surface areas which were decreased along with the increase of the diameter. The magnetic properties of the as-obtained hematite hollow spheres were investigated. The result showed that the coercivity and saturated magnetization were increased along with the increase of the shell thickness, and the remanent magnetization was increased along with the decrease of the diameter.

关键词： hollow spheres hematite shell thickness BET special surface area magnetization properties

A characterization of solvability for a class of networks

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Science China(Information Sciences) 2012年第4期55卷 747-754页

作者： YUAN Chen & KAN HaiBin Shanghai key lab of intelligent Information Processing,School of Computer Science,Fudan University,Shanghai 200433,China Shanghai key lab of intelligent Information Processing School of Computer Science Fudan University Shanghai China

In this paper,we find that Property P can be generalized to characterize the solvability of a kind of networks with any number of sources,thus partially answering the open problem as to whether there are properties similar to Property P to characterize the solvability of some *** an application,for a given integer n,we construct such a solvable network that has no solvable solution if its alphabet size is less than n.

关键词： capacity coding capacity network coding solvability