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世界地震工程 2003年第1期19卷 25-30页

作者：陈艳艳王光远北京工业大学交通工程研究所北京100022 哈尔滨工业大学土木工程学院黑龙江哈尔滨150090

借鉴可靠度理论,通过震后交通需求及道路震后通行能力的随机性分析并考虑灾后不同阶段的交通供需特性,进行交通系统震后阻塞发生的概率分析及引发损失期望预测,从而为抗震加固决策及震后交通管理及修复提供依据。

关键词：道路交通系统震害阻塞概率地震引发损失期望铁路抗震加固震后应急系统

research on Double Sided Polishing technology for Silicon Wafer

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Research on Double Sided Polishing Technology for Silicon Wa...

The 9th International Symposium on Advances in Abrasive technology

作者： X.D. Hu, Y.F. Jin, W. Li , X.Z. Hu The MOE Key Laboratory of Mechanical manufacture and Automation, zhejiang university of technology Engineering College, zhejiang Ocean university

Double sided polishing is one of the main processes to access ultra-smooth surface of semiconductor wafers. This paper introduces the key technologies of developing double sided polishing machine for 4" wafers, and analyzed the polishing processes for 4" Si wafers base on the machine developed. Experiment results showed that the equipment can finish a batch 4" Si wafers (thickness 0.5mm) in 40 minutes, the average removal rate is 0.25μ/min, and the surface quality can reach Ra 0.401nm, GFLD ±1.63μm.

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Impact of Frequency of Intercourse on Erectile Dysfunction: A Cross-sectional Study in Wuhan, china

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Journal of Huazhong university of Science and technology(Medical Sciences) 2012年第3期32卷 396-399页

作者：秦战停田斌群王行环刘同族白锦兵 Urology and Andrology research center Zhongnan Hospital Wuhan University School of Nursing Tianjin Medical University

This study was aimed to investigate the influence of coital frequency and masturbation on erectile dysfunction （ED） in Chinese patients. A total of 332 male outpatients with or without ED and volunteers were recruited from Zhongnan Hospital, Wuhan university, china. ED was assessed by using the five-item International Index of Erectile Function scale and the frequency of intercourse by patients＇ self-report. After adjusting for lifestyle factors and diseases-related factors, the analyses showed that coital and masturbation in a certain frequency tended to decrease the risk of ED. Men reporting intercourse once a week had lower risk of ED than those did less than once a week, with age, hyperlipidemia, hy-pertension, diabetes, body mass index, smoking, and drinking as covariates （P=0.67, adjusted odds ratio [OR] = 0.84; 95% confidence interval [CI]： 0.37-1.88）. For those reporting coital frequency two times per week and three or more times per week, there were 63% （adjusted OR = 0.37; 95% CI： 0.18-0.77） and 85% （adjusted OR = 0.15; 95% CI： 0.07-0.35） lower risk of ED than those reporting intercourse frequency less than once per week, respectively （P〈0.05）. Results indicated that maintaining a regular frequency of intercourse can reduce the risk of ED for males aged among 30 to 75 years.

关键词： frequency intercourse erectile dysfunction outpatients cross-sectional study

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人肝肿瘤细胞HepG2最佳培养条件探讨

同济医科大学学报 2001年第4期30卷 319-320页

作者：甘起霓鲁文清张悦华中科技大学同济医学院环境卫生学教研室武汉430030 华中科技大学同济医学院实验医学研究中心免疫室武汉430030

人肝肿瘤细胞株 Hep G2可应用于体外遗传毒理学试验等多种研究。但其培养难度较大 ,通过对 Hep G2细胞在不同培养条件下生长状态的对比 ,探讨了 Hep G2细胞培养增殖的最佳条件。结果表明在下列情况下细胞能顺利传代生长 :复苏时需 2 0 ... 详细信息

人肝肿瘤细胞株 Hep G2可应用于体外遗传毒理学试验等多种研究。但其培养难度较大 ,通过对 Hep G2细胞在不同培养条件下生长状态的对比 ,探讨了 Hep G2细胞培养增殖的最佳条件。结果表明在下列情况下细胞能顺利传代生长 :复苏时需 2 0 %胎牛血清 ,接种密度为 1× 10 4/ cm2 ,需在含 5 % CO2 环境下 ;培养时需 10 %胎牛血清 ,可在无 5 %

关键词：人肝肿瘤细胞株细胞培养肝肿瘤

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聚四氟乙烯防水透湿层压织物的研制与开发

上海纺织科技 2003年第5期31卷 53-54页

作者：黄机质张建春郝新敏王锋东华大学纺织学院上海200051 总后军需装备研究所北京100088 上海新特纺织材料研究中心上海200082

阐述了研制的聚四氟乙烯防水透湿层压织物生产的技术关键 ,介绍了该产品的国内外应用和开发概况 ,认为有必要加强这一高科技产品的研究和开发力度。

关键词：聚四氟乙烯防水透湿层压织物生产技术产品开发 PTFE微孔薄膜

The Mitochondrial tRNAGlu A14693G Mutation May Influence the Phenotypic Manifestation of Deafness-Associated 12S rRNA A1555G Mutation in a Chinese Family

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The Mitochondrial tRNAGlu A14693G Mutation May Influence the...

中国遗传学会第八次代表大会暨学术讨论会

作者： Yu Ding~1 Jian Xin Lu~1 Bo Bei Chen~2 Yong Yan Li~1 Jun Yan You~1 Li Yang~3 Min-Xin Guan~(1,3,4) 1.zhejiang Provnical Key Laboratory of Medical Genetics,Wenzhou Medical College,Wenzhou,zhejiang 325035,china 2.Department of Otolaryngology,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou,zhejiang 325027,china 3.Division and Program in Human Genetics and center for Hearing and Deafness research,Cincinnati Children’s Hospital Medical center,Cincinnati,Ohio,OH 45229,USA 4.Department of Pediatrics,university of Cincinnati College of Medicine,Cincinnati,Ohio,OH 45229,USA

Mutations in mitochondrial DNA(mtDNA)have been found to be associated with sensorineural hearing *** report here on the clinical,genetic,and molecular characterization of a four generation Chinese family with aminoglycoside-induced and nonsyndromic hearing loss. Clinical evaluation revealed the variable phenotype of hearing loss including bilateral,severity, age-at-onset and audiometric configuration in these *** penetrance of hearing loss in this family is 26%,while the aminoglycoside is excluded,the penetrance is 11%.Sequence analysis of the complete mitochondrial DNA in this pedigree showed the presence of the deafness-associated 12S rRNA A1555G mutation,in addition to distinct sets of mtDNA polymorphism belonging to East Asian haplogroup ***,this homoplasmic mtDNA variant was absent among 156 unrelated Chinese *** further understand the role of nuclear modified genes involved in the phenotypic manifestation of deafness-associated 12S rRNA A I555G mutation,we perform the PCR amplification of GJB2 gene and TRMU gene in the affected relatives of this ***,the absent of variants in GJB2 and TRMU indicated that GJB2 and TRMU may not be the modified factors of the phenotypic effects of the A1555G mutation in those *** these variants,the homoplasmic A14693G mutation in the tRNAGlu is of special interest as it was implicated to be associated with various mitochondrial disorders,such as MELAS,hypertension,and may have a modified role in the phenotypic expression of LHON-associated ND1 G3460A mutation in Chinese *** mutation is located at the TΨC-loop,at conventional position of 54 of *** uridine at this position(U54),which is highly conserved from bacteria to human species,has been shown to be important for tRNA structure and *** alteration of structure of the tRNA by these mtDNA mutations may lead to a failure in tRNA metabolism,thereby causing impairment of mitochondrial ***,mitochondri

关键词： Hearing loss Mitochondrial DNA Mutation tRNA variant Aminoglycoside Penetrance

Elevation of IAA Level in Ovule Epidermal Cells by Targeted Expression of an Auxin Biosynthetic Gene at the Stage of Fiber Cell Initiation Improves Fiber Yield and Fineness of Cotton

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Elevation of IAA Level in Ovule Epidermal Cells by Targeted ...

第十二届全国植物基因组学大会

作者： Mi Zhang~1,Xuelian Zheng~1,Shuiqing Song~1,Qiwei Zeng~1,Lei Hou~1,Demou Li~1,Juan Zhao~1,Yuan Wei~1, Xianbi Li~1,Ming Luo~1,Yuehua Xiao~1,Xiaoying Luo~1,Jinfa Zhang~2,Chengbin Xiang~3,Yan Pei~(1*) 1.Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture,Biotechnology research center, Southwest university,Chongqing 400716,china 2.Department of Plant and Environmental Sciences,New Mexico State university, Las Cruces,NM 88003,USA 3.School of Life Sciences,university of Science and technology of china,Hefei,Anhui 230027,china

Cotton(Gossypium hirsutum L.) is the most important textile fiber crop in the ***,the capacity of conventional breeding to simultaneously improve both the yield and quality of cotton fiber is limited. Based on our observation that the plant hormone IAA is highly accumulated in cotton fiber initials,we developed a novel strategy to improve fiber yield and quality by genetically increasing IAA level in the ovule *** achieve this goal,we analyzed expression patterns of 16 promoters and selected a seed coat specific gene promoter,FBP7,to drive an auxin biosynthesis gene,iaaM,in *** iaaM transcript level

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An Efficient Spam Filtering Algorithm Based on NPE

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An Efficient Spam Filtering Algorithm Based on NPE

2008 IEEE International Symposium on Knowledge Acquisition and Modeling Workshop(KAM 2008)

作者： Ziqiang Wang School of Information Science and Engineering Henan university of technology Zhengzhou chinaXia Sun School of Information Science and Engineering Henan university of technology Zhengzhou china

With the popularization of the Internet,it is challenging to develop spam filters that can effectively eliminate the increasing volumes of unwanted mails automatically before they enter a user’s *** efficiently solve the spam filtering problems,a spam mail filtering method based on neighborhood preserving embedding （NPE） and support vector machines （SVM） classifier is proposed in this *** results show that the proposed algorithm outperforms other related spam filtering methods.

关键词： spam filtering neighborhood preserving embedding support vector machine text categorization

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乌鳢致病诺卡氏菌的鉴定及系统发育分析

水产学报 2008年第3期32卷 449-454页

作者：王国良徐益军金珊宁波大学生命科学与生物工程学院教育部应用海洋生物技术重点实验室宁波大学生命科学与生物工程学院教育部应用海洋生物技术重点实验室浙江宁波315211 宁波大学医学院

2006年6月,浙江萧山养殖乌鳢(Ophiocephalus argusCantor)暴发一种类似细菌性的结节病,从患典型症状乌鳢肝脏和肾脏中分离到纯度一致的菌株W060622。形态结构观察显示,菌株W060622革兰氏阳性,好氧,具有弱抗酸性,菌体呈长或短杆状,或细... 详细信息

2006年6月,浙江萧山养殖乌鳢(Ophiocephalus argusCantor)暴发一种类似细菌性的结节病,从患典型症状乌鳢肝脏和肾脏中分离到纯度一致的菌株W060622。形态结构观察显示,菌株W060622革兰氏阳性,好氧,具有弱抗酸性,菌体呈长或短杆状,或细长分枝状。常规生理生化试验表明,该菌株具有诺卡氏菌属(Nocardia)的基本特性。对其16S rRNA基因进行PCR扩增、测序及系统发育分析,结果表明,该菌株与诺卡氏菌属的菌株亲缘关系最近,与鱼诺卡氏菌(Nocardia serioleaJCM 3360T)的16S rRNA基因序列相似性达99.9%。据此鉴定菌株W060622为鱼诺卡氏菌(Nocardia seriolea)。

关键词：乌鳢鱼诺卡氏菌结节病生理生化 16SrRNA 系统发育分析

Genotypic diagnosis of long QT syndrome by analysis of candidate genes

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Journal of Pharmaceutical Analysis 2009年第4期21卷 222-224,229页

作者： Jiang-fang Lian1,Chen Huang2,Xiao-yan Huang1,Ying Wang1,Shi-jun Ge1,Jian-qing Zhou1 1.The Li Huili Hospital,Medical School of Ningbo university,Ningbo 315041 2.Medical School of Xi’an Jiaotong university,Xi’an 710061,china Li Huili Hospital Medical School of Ningbo University Ningbo 315041 China Medical School of Xi'an Jiaotong university Xi'an 710061 China

Objective To diagnose 6 LQTS families by genetic *** A total aof 6 LQTS pedigrees with 43 family members were brought together for genetic diagnosis by using short-sequence tandem-repeat(STR)markers or *** DNA was extracted from blood samples by standard *** markers or KCNQ1,KCNH2 and SCN5A were *** haplotype analysis for LQTS was *** the family got the negative haplotype analysis,the sequencing was *** LQTS patients were always linkaged with the SCN5A gene in family ***2 was linkaged with the disease in family 2 to 5.21 gene carriers were identified from these 5 families.A mutation(A561V-KCNH2)was only found in the proband of family 6 and an SNP(G1691A)was found in all the members of the *** Genetic diagnosis can not only improve presymptomatic diagnosis,but also provide the basis for personal therapy and research on disease-causing mutations.

关键词： cardiology long QT syndrome short tandem repeat linkage analysis sequencing