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Role of oxyR from Sinorhizobium meliloti in Regulating the Expression of Catalases

Acta Biochimica et Biophysica Sinica 2005年第6期37卷 421-428页

作者： Li EUO~(1.3) Ming-Sheng QI~(1.3) Shi-Yi YAO~2 Hai-Ping CHENG~2 Jia-Bi ZHU~1 Guan-Qiao YU~1 1 national laboratory of plant molecular genetics, institute of plant physiology and ecology. shanghai institutes for biological sciences.chinese academy of sciences. shanghai 2.0032. china 2 biological sciences.Department, Lehman College, the City University of New York, Bronx, NY 10468. USA, 3 Graduate School of the chinese academy of sciences. shanghai 2.0032. china nationallaboratoryofplantmoleculargenetics InstituteofPlantPhysiologyandEcologyShanghaiInstitutesforBiologi

The process of symbiotic nitrogen fixation results in the generation of reactive oxygen speciessuch as the superoxide anion（O2-）and hydrogen peroxide（H2O2）.The response of rhizobia to these toxicoxygen species is an important factor in nodulation and nitrogen *** Sinorhizobium meliloti,oneoxyR homologue and three catalase genes,katA,katB,and katC were detected by sequence *** gene is located next to and divergently from katA on the *** investigate the possible rolesof oxyR in regulating the expression of catalases at the transcriptional level in ***,an insertion mutantof this gene was *** mutant was more sensitive and less adaptive to H2O2than the wild typestrain,and total catalase/peroxidase activity was reduced approximately fourfold with the OxyR mutationrelative to *** activities of KatA and KatB and the expression of katA::lacZ and katB::lacZ promoterfusions were increased in the mutant strain compared with the parental strain grown in the absence of H2O2,indicating that katA and katB are repressed by ***,when exposed to H2O2,katA expressionwas also increased in both *** and Escherichia *** exposed to H2O2,OxyR is convertedfrom a reduced to an oxidized form in *** concluded that the reduced form of OxyR functions as arepressor of katA and katB ***,in the presence of H2O2,reduced OxyR is converted to theoxidized form of OxyR that then results in increased katA *** further showed that oxyR expressionis autoregulated via negative feedback.

关键词： Sinorhizobium meliloti OxyR catalase katA H2O2

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Bacterial Type Ⅰ Glutamine Synthetase of the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32,is the Only Enzyme Responsible for Glutamine Synthesis under Physiological Conditions

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Acta Biochimica et Biophysica Sinica 2006年第12期 821-830页

作者： Wen-Tao PENG~(1&) Jin WANG~1 Ting WU~2 Jian-Qiang HUANG~1 Jui-Shen CHIAO~1 Guo-Ping ZHAO~(1,3,4) 1 laboratory of molecular Microbiology,institute of plant physiology and ecology.shanghai institutes for biological sciences.chinese academy of sciences.shanghai 2.0032.china 2 Department of Medicinal Chemistry,School of Pharmacy,Fudan University,shanghai 2.0032.china 3 State key laboratory of Genetic Engineering/Department of Microbiology,School of Life Science,Fudan University,shanghai 200433,china 4 laboratory of Health and Disease Genomics,chinese national Haman Genome Center at shanghai,shanghai 201203,china

The structural gene for glutamine synthetase,glnA,from Amycolatopsis mediterranei U32 wascloned via screening a genomic library using the analog gene from Streptomyces *** clone wasfunctionally verified by complementing for glutamine requirement of an Escherichia coli glnA null mutantunder the control of a lac *** analysis showed an open reading frame encoding a protein of466 amino acid *** deduced amino acid sequence bears significant homologies to other bacterialtype Ⅰ glutamine synthetases,specifically,71% and 72% identical to the enzymes of *** and Myco-bacterium tuberculosis,*** of this glnA gene in *** U32 led to glutamineauxotrophy with no detectable glutamine synthetase activity in *** contrast,the cloned glnA+gene cancomplement for both phenotypes in *** thus suggested that in *** U32,the glnA geneencoding glutamine synthetase is uniquely responsible for in vivo glutamine synthesis under our laboratorydefined physiological conditions.

关键词： glnA glutamine synthetase Amycolatopsis mediterranei rifamycin SV

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Activities of floral meristem identity genes in “cauliflower-like” inflorescences of ago1 mutants

Activities of floral meristem identity genes in “cauliflowe...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Han Wang and Yuke He* national key laboratory of plant molecular genetics, institute of plant physiology and ecology. shanghai institutes for biological sciences. chinese academy of sciences. 300 Fenglin Road, shanghai 2.0032. china

＜正＞Floral development is an important process in the life cycle of plants and floral meristem genes play a key role in the transition to reproductive stage, such as LFY, AP1 and CAL[1,2,3]. MicroRNAs are endogenous... 详细信息

关键词： Arabidopsis Floral meristem genes AGO1 AP1 CAL

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集胞蓝藻PCC6803含疏水亚基的NAD(P)H脱氢酶亚复合体的分离

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生物化学与生物物理学报 2003年第8期35卷 723-727页

作者：邓勇叶济宇米华玲沈允钢中国科学院上海生命科学研究院植物生理生态研究所、植物分子遗传国家重点实验室中国科学院上海生命科学研究院植物生理生态研究所、植物分子遗传国家重点实验室上海2.0032

利用离子交换与凝胶过滤层析 ,从n dodecylβ D maltoside(DM)处理的集胞蓝藻SynechocystisPCC6 80 3细胞粗提液中 ,首次分离到两个包含NDH疏水亚基NdhA的亚复合体。酶活性分析表明 ,分离到的NDH亚复合体具有NADPH 氮蓝四唑 (NBT)氧化... 详细信息

利用离子交换与凝胶过滤层析 ,从n dodecylβ D maltoside(DM)处理的集胞蓝藻SynechocystisPCC6 80 3细胞粗提液中 ,首次分离到两个包含NDH疏水亚基NdhA的亚复合体。酶活性分析表明 ,分离到的NDH亚复合体具有NADPH 氮蓝四唑 (NBT)氧化还原酶活性 ,以NADPH为电子供体可以还原铁氰化钾、二溴百里香醌 (DBMIB)、二氯酚靛酚 (DCPIP)、duroquinone以及UQ 0等质醌类电子受体。

关键词：集胞蓝藻 NAD(P)H脱氢酶疏水亚基

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Regulation of rice inclination by an IAA amino synthetase

Regulation of rice inclination by an IAA amino synthetase

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2007中国植物生理学会全国学术会议

作者： Shuqing Zhao Hongwei Xue (national key laboratory of plant molecular genetics,institute of plant physiology and ecology.shanghai institute for biological sciences.chinese academy of Scienses,shanghai,china)

The angle of rice leaf inclination is an important agronomic ***,few mutants with altered leaf angles were reported,however,the molecular mechanism remains to be *** characterized a rice mutant leaf inclination 1,lc1 from shanghai T-DNA Insertion Population(SHIP),and phenotypic analysis confirmed the enlarged leaf angels were due to the overexpression of the OsGH3.1 ***3.1 encodes

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Negative Regulation of Receptor Signaling by ERS1

Negative Regulation of Receptor Signaling by ERS1

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上海市植物生理学会成立三十周年庆祝活动暨第三届上海市植物生理学青年学术研讨会

作者： Qian Liu, Chan Xu, and Chikuang Wen* national laboratory of plant molecular genetics, institute of plant physiology and ecology. shanghai institutes for biological sciences. chinese academy of sciences. shanghai 2.0032

Previous studies revealed that the five Arabidopsis ethylene receptor genes have partially redundant functions and negatively regulate ethylene response. Genetic redundancy limits further

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Study of Complex Redundancy-Pleiotropy Network of Arabidopsis Ethylene Receptors

Study of Complex Redundancy-Pleiotropy Network of Arabidopsi...

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2010中国青年遗传学家论坛

作者： Qian Liu and Chi-Kuang Wen national key laboratory of plant molecular genetics,institute of plant physiology and ecology.shanghai institutes for biological sciences.chinese academy of sciences.300 Fenglin Rd,shanghai 2.0032.china

Ethylene responses are modulated by a family of five ethylene receptor members in *** five receptors are genetically redundant while not functionally exchangeable;how the five receptor members may act coordinately remains *** addressed this question by examining the signal output of transmitter-on ETR1 or ERS1 in the absence of other wild-type receptors or in the presence of a wild-type receptor *** to a singling active state by silver or 1-MCP, ETR1 alone was able to reverse ethylene growth inhibition to a great extent while ERS1 was ***-1, ersl-1,and erslC65Y are transmitter-on receptor ***-1 substantially repressed ethylene responses in quintuple receptor knockout mutants while ersl-1 did *** inhibition of ethylene responses by ersl-1 is mainly dependent on ETR1 or ***4 had a greater role than ETR1 in erslC65Y *** lack of both ETR1 and EIN4 abolished the ability of erslC65Y in the repression of ethylene,*** show that the wild-type receptors are not dispensable to the signal output of a transmitter-on ethylene receptor,implying collaborative receptor *** collaborations may facilitate a plant to make corresponding responses to a wide range of ethylene *** redundancy and functional divergence of the five receptor members are essential to the maintenance of collaborative receptor signaling.

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The differential reversion of ethylene response by ETRl and ERS1 implies functional significance of ethylene receptor collaboration

The differential reversion of ethylene response by ETRl and ...

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2011全国植物生物学研讨会

作者： Qian Liu and Chi-Kuang Wen~* national key laboratory of plant molecular genetics,institute of plant physiology and ecology. shanghai institutes for biological sciences.chinese academy of sciences.300 Fenglin Rd,shanghai 2.0032.china

In Arabidopsis,the ethylene signal is mediated by a family of five receptor members while little is known about the nature of the receptor signal and how the five receptor members may act *** we show that ETR1,but not ERS 1,was able to reverse ethylene growth inhibitions to a great extent upon 1-MCP,an ethylene antagonist,treatment in the absence of other ***-1,ers 1-1,and ers 1 are ethylene-insensitive ***-1 substantially repressed various ethylene responses independent of other receptor *** inhibition of ethylene response

关键词： Ethylene etr1-1 ers1-1 ers1C65Y receptor collaborations

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Global Expression Pattern Analysis of Rice RLK Coding Genes in Developing Seeds and in Response to Stress Conditions

Global Expression Pattern Analysis of Rice RLK Coding Genes ...

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Linlin Gao,Hong-Wei Xue~* national key laboratory of plant molecular genetics,institute of plant physiology and ecology. shanghai institutes for biological sciences.chinese academy of sciences.300 Fenglin Road, 2.0032.shanghai,china

The receptor-like kinase(RLK) plays critical roles in plant growth,development,and stresses responses,through sensing the extracellular signals and activating the downstream signal transduction by phosphorylating the ... 详细信息

关键词： receptor-like kinase rice seed development stress

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Proteomic analysis of rice plasma membrane in early defense response to bacterial blight and research on PM associated proteins

Proteomic analysis of rice plasma membrane in early defense ...

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中国遗传学会七届二次青年研讨会

作者： Fang Chen,Yuexin Yuan,Qun Li,Zuhua He national key laboratory of plant molecular genetics,institute of plant physiology and ecology.shanghai institutes for biological sciences.The chinese academy of sciences.shanghai 2.0032.china

plant plasma membrane (PM) proteins play important roles in signal perception and transduction during defense response to an attacking pathogen. Through using an improved method of PM protein preparation, and PM-bound GFP fusion protein as a visible marker for routine experiments, we conducted PM proteomics analysis of the rice suspension cells expressing the disease resistance gene Xa21, to identify PM components involved in the early defense response to bacterial blight (Xanthomonas oryzae pv. oryzae). A total of 20 regulated protein spots were observed on 2-D gels of PM fractions at 12h and 24h after pathogen inoculation, of which some were differentially regulated between the incompatible and compatible interactions mediated by Xa21. Eleven protein spots with predicted functions in plant defense were identified by MS/MS, with 9 putative PM-associated proteins including H+-ATPase, protein phosphatase, hypersensitive-induced response protein (OsHIR1), prohibitin (OsPHB2), zinc finger and C2 domain protein, universal stress protein (USP), and LMW heat shock protein. OsHIR1 could be phosphorylated by the microbe challenge, leading to two differentially accumulated protein spots. Finally, gene expression analysis of regulated proteins was performed by RT-PCR, which showed that most, if not all, different proteins’ mRNA level were consistant with their protein level. Besides, some research was also performed on a membrane protein kinase involved in disease resistance. Our study would provide a starting point for the functionality of PM proteins in the rice defense.

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