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Stress responsive gene CIPK14 is involved in phytochrome A-mediated far-red light inhibition of greening in Arabidopsis

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Science china(Life Sciences) 2010年第11期53卷 1307-1314页

作者： QIN YuZhi2,3, GUO Ming1, LI Xu1, XIONG XingYao2,3, HE ChangZheng2,3, NIE XianZhou4 & LIU XuanMing1 1college of Life Science and Biotechnology, hunan university, changsha 410082, china 2college of horticulture and landscape, hunan agricultural university, changsha 410128, china 3hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, hunan agricultural university, changsha 410128, china 4Potato Research Centre, Agriculture and Agri-Food Canada, Fredericton, New Brunswick E3B 4Z7, Canada 2. college of horticulture and landscape Hunan Agricultural University Changsha 410128 China3. Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization Hunan Agricultural University Changsha 410128 China1. College of Life Science and Biotechnology Hunan University Changsha 410082 China4. Potato Research Centre Agriculture and Agri-Food Canada Fredericton New Brunswick E3B 4Z7 Canada

In this study, we show that CIPK14,a stress responsive CBL-interacting protein kinase gene,is involved in phytochrome A-mediated far-red light inhibition of greening in Arabidopsis seedlings. The CIPK14-impairment mutant cipk14 grown in continuous far-red (FR) light did not show greening when exposed to white light illumination for 15 h. By contrast, the FR-grown phytochrome A null mutant phyA greened within 0.5 h of exposure to white light. Although greening of Col-4 (wild-type) was not completely abolished by FR, it exhibited a significantly decreased greening capacity compared with that of phyA. Further analyses demonstrated that the expression of protochlorophyllide reductase (POR) genes was correlated with the greening ability of the genotypes. In addition, CIPK14 appeared to be regulated by both the circadian clock and PhyA. Taken together, these results suggest that CIPK14 plays a role in PhyA-mediated FR inhibition of seedling greening, and that a Ca-related kinase may be involved in a previously undefined branch point in the phytochrome A signaling pathway.

关键词： chlorophyll CIPK14 far-red light inhibition of greening POR phytochrome A

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Fixed Ammonium in Major Types of Paddy Soils in hunan Province, china

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Pedosphere 2003年第3期13卷 199-208页

作者： ZHANG YANGZHU, LIAO JIPEI, SUN YUHUAN, FENG YUEHUA and HUANG YUNXIANGcollege of Resources and Environment, hunan agricultural university, changsha 410128 (china) college of Resources and Environment Hunan Agricultural University Changsha 410128

The contents, affecting factors, seasonal changes and availability of fixed ammonium in major types ofpaddy soils derived from different parent materials in hunan Province, china, were studied using the Silva-Bremner method by laboratory and pot experiments. Results showed that the content of fixed ammoniumin the plough horizons ranged from 88.3 mg kg-1 to 388.1 mg kg-1, with 273.2 ± 77.7 mg kg-1 on average,accounting for 11.2% of total soil N on average. Content of fixed ammonium decreased in the order of newlylacustrine clayey paddy soil ＞ alluvial sandy paddy soil ＞ purple clayey paddy soil ＞ newly alluvial sandypaddy soil ＞ yellow clayey paddy soil ＞ reddish-yellow clayey paddy soil ＞ granitic sandy paddy soil. Therewere four distribution patterns of fixed ammonium in the profiles to 1-m depth, i.e., increase with the depth,decrease with increasing depth, no distinct change with the depth, and abrupt increase or decrease in somehorizon. Percentage of fixed ammonium in total N increased with the depth in most of the soils. Fixationof NH4+ by soil was higher at 30 ℃ than at 20 ℃ and 40 ℃, and continuous submergence benefited thefixation of NH4+ in newly alluvial sandy paddy soil, purple clayey paddy soil and alluvial sandy paddy soil,while alternating wetting and drying contributed to the fixation of NH4+ in yellow clayey paddy soil *** ammonium content in the test paddy soils was significantly correlated with ＜ 0.01 mm clay content(P ＜ 0.05), but not with ＜ 0.001 mm clay content, total N, organic N and organic matter. Fixed ammoniumcontent varied with rice growth stages. Application of N fertilizer promoted fixation of NH4+ by soil, and Nuptake by rice plant promoted release of fixed ammonium from the soil. Recently fixed ammonium in paddysoil after N fertilizer application was nearly 100% available to rice plant, while native fixed ammonium wasonly partly available, varying with the soil type and rice type.

关键词： affecting factors bioavailability fixed ammonium hunan province paddysoil

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Exogenous ethylene influences flower opening of cut roses (Rosa hybrida) by regulating the genes encoding ethylene biosynthesis enzymes

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Science china(Life Sciences) 2005年第5期48卷 434-444页

作者： MA Nan1, CAI Lei1, LU Wangjin2, TAN Hui1 & GAO Junping1 1. Department of Ornamental horticulture and landscape Architecture, china agricultural university, Beijing 100094, china 2. Department of horticulture, South china agricultural university, Guangzhou 510642,china 1. Department of Ornamental horticulture and landscape Architecture China Agricultural University 100094 Beijing China 2. Department of horticulture South China Agricultural University 510642 Guangzhou China

The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars, ‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process is inhibited by ethylene. Ethylene production and 1- aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment, ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage (stage 3) in ‘Samantha’, and they were much more dramatically enhanced and peaked at the later stage (stage 4) in ‘Kardinal’ than control during vasing. cDNA fragments of three Rh-ACSs and one Rh- ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-in- duced expression patterns of Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in ‘Kardinal’ than that of ‘Samantha’. As an ethylene action inhibitor, STS at concentration of 0.2 mmol/L generally inhib-ited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in ‘Kardinal’. Our results suggests that ‘Kardinal’ is more sensitive to ethylene than ‘Samantha’; and the changes of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in ‘Samantha’ and the inhibition in ‘Kardinal’. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding.

关键词： cut rose (Rosa hybrida), flower opening, ethylene, 1-aminocyclopropane-1-carboxylic acid (ACC), ACC synthase (ACS), ACC oxidase (ACO), gene expression.

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Ectopic expression of a hyacinth AGL6 homolog caused earlier flowering and homeotic conversion in Arabidopsis

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Science china(Life Sciences) 2007年第5期50卷 676-689页

作者： FAN JinHui1, LI WenQing2, DONG XiuChun1, GUO Wei1 & SHU HuaiRui3 1 college of Forestry, Shandong agricultural university, Taian 271018, china 2 college of Biological Science, china agricultural university, Beijing 100094, china 3 college of horticulture Science and Engineering, Shandong agricultural university, Taian 271018, china 1. college of Forestry Shandong Agricultural University Taian 271018 China 2. college of Biological Science China Agricultural University Beijing 100094 China 3. college of horticulture Science and Engineering Shandong Agricultural University Taian 271018 China

MADS-box genes are involved in floral organ *** we report thatan AGL6(Agamous-like 6)-like MADS-box gene,H0AGL6,was isolated from Hyacinthus *** pattern analysis demonstrated that H0AGL6 transcript was detected in inflorescencebuds,tepals,carpels and ovules,but not in stamina,leaves or *** Arabidopsis plantsectopically expressing H0AGL6 exhibited novel phenotypes of significantly reduced plantsize,extremely early flowering,and losing inflorescence *** addition,wide homeoticconversion of sepals,petals,and leaves into carpel-like or ovary structures,and disappearance ornumber reduction of stamens in 35S::HoAGL6 Arabidopsis plants were also ***-PCR analysisindicated that the expressions of flowering time gene SOC1 and flower meristem identity gene LFYwere significantly up-regulated in 35S::Ho4GL6transgenic Arabidopsis plants,and the expressionlevels of floral organ identity genes AG and SEP1 in leaves were also *** resultsindicated that H0AGL6 was involved in the regulation of flower transition and flower organformation.

关键词： AGL6 homologue, flower development, flowering time, MADS-box genes, Hyacinthus orientalis

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Effects and Mechanisms of P and K Nutrients on Yield and Protein Content of Fodder Rice

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agricultural Sciences in china 2002年第4期1卷 432-437页

作者： TANG Xiang-ru and YU Tie-qiao( Department of Agronomy, South china agricultural university, Guangzhou 510642 , P.R. china college of Plant Science and Technology, hunan agricultural university, changsha 410128 , P.R. china) Department of Agronomy South China Agricultural University Guangzhou 510642 P. R. China college of Plant Science and Technology Hunan Agricultural University Changsha 410128 P.R. China college of Plant Science and Technology Hunan Agricultural University Changsha 410128 P.R. China

Effects and mechanisms of P and K nutrients on yield and protein content of Weiyou 56, a fodder hybrid rice combination,were studied through pot experiment and biochemical analysis. The results showed that the increase of P and K nutrients enhanced the activities of PEP carboxylase (PEPC), glutamine synthase (GS) and sucrose phosphate synthase (SPS) in leaves, sucrose synthase (SS), ADP glucose pyrophosphorylase (ADPGP) and GS in grains, and the chlorophyll content in leaves, soluble sugar and starch content in grains, protein N and total N content in leaves and grains. Howerer, they decreased soluble sugar content in leaves and led to an increase of protein content in brown rice, biomass, grain yield and harvest index. Excessive P nutrients slightly reduced SPS and ADPG activity in leaves and grains respectively.

关键词： Superphosphate Potassium chloride Fodder rice Yield Protein content Enzyme activity

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EST-derived microsatellites in P seudosciaena crocea and their applicability to related species

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Acta Oceanologica Sinica 2010年第6期29卷 83-91页

作者： YE Hua WANG Xiaoqing GAO Tianxiang WANG Zhiyong college of Animal Science and Technology Hunan Agricultural University Changsha 410128 China Key Laboratory of Science and Technology for Aquaculture and Food Safety Fisheries College/Fisheries Biotechnology Institute Jimei University Xiamen 361021 China Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 China

Simple sequence repeat （SSR） markers were obtained for the large yellow croaker Pseudosciaena crocea using 1 205 expressed sequences tags （ESTs） from the NCBI *** for 48 ESTSSR loci were designed and screened with 30 *** specimens captured from Guanjingyang sea area in Fujian Province of *** of the loci were polymorphic,which were amplified with 3 to 11 alleles per locus and the mean of *** observed and expected heterozygosity per locus ranged from 0.091 to 0.844 （mean 0.544） and from 0.118 to 0.892 （mean 0.644）,*** information content （PIC） ranged from 0.115 to 0.866 （mean 0.593）.The results for cross-species amplification of the 16 large yellow croaker EST-SSRs on ***,***,***,*** and *** revealed that 14,12,11,7 and 6 loci were successfully amplified with 1 to 10 alleles with an average of 4.5 per locus,respectively,which are suitable for population genetics studies of these species and useful for phylogenetic relationship analysis among these ***,this study provides a set of type I markers for population genetics studies and genome mapping for large yellow croaker and its closely related species.

关键词： Pseudosciaena crocea EST-SSRs genetic diversity cross-species amplification

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Analysis of Calcium Content, Hormones, and Degrading Enzymes in Tomato Pedicel Explants During Calcium-Inhibited Abscission

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agricultural Sciences in china 2009年第5期8卷 556-563页

作者： XU Tao, LI Tian-lai and QI Ming-fang college of horticulture, Shenyang agricultural university, Shenyang 110161, P.R.china [a]college of horticulture Shenyang Agricultural University Shenyang 110161 P.R. China

This study was designed to analyze the changes of phytohormone concentrations, calcium fraction, and the activities of degrading enzymes during calcium-inhibited and ethyleneglycol-bis-（β-aminoethyl ether）N, N＇-tetraacetic acid （EGTA）-induced abscission of tomato pedicel explants. Added calcium caused an increase in the total calcium content within the abscission zone and produced a corresponding reduction （20%） in pedicel explant abscission. As expected, EGTA treatment produced the opposite effect and resulted in a decrease in the total calcium content, while accelerating abscission of pedicel explants. Hormone analysis revealed that indole-3-acetic acid （IAA） concentrations in the abscission zone first decreased and then increased before the occurrence of abscission in all treatments, with the greatest effect produced by addition of EGTA. Similarly, abscisic acid （ABA）, and gibberellin （GA1＋3） concentrations, and ethylene production were elevated in the abscission zone during the first 16 h before abscission when explants imbibed EGTA. With calcium treatment, the concentrations of ABA, ethylene, and GA1＋3 also increased in pedicels throughout the first 16 h following exposure, but the increase was slower and less dramatic than with EGTA. Both cellulase and polygalacturonase were induced in the explants during abscission and the activities were also strengthened by treatment with EGTA. Calciumtreated explants produced lower hydrolysing enzyme activities than controls throughout abscission. Calcium played a role of mediating hormone balance and degrading enzymes activities and affected on abscission.

关键词： abscission calcium phytohormone degrading enzymes tomato

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Development of simple sequence repeats (SSR) markers of ramie and comparison of SSR and inter-SSR marker systems

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Progress in Natural Science:Materials International 2005年第2期15卷 42-47页

作者： ZHOU Jianlin1*, JIE Yucheng2*, JIANG Yanbo2, ZHONG Yingli1, LIU Yunhai1 and ZHANG Jian1**(1. Department of Biochemistry and Molecular Biology, college of Life Science, hunan Normal university, changsha 410081, china 2. Institute of Bast Fiber Crops, Chinese Academy of agricultural Science, changsha 410006, china) Department of Biochemistry and Molecular Biology College of Life Science Hunan Normal University Changsha 410081 China. Institute of Bast Fiber Crops Chinese Academy of Agricultural Science Changsha 410006 China

Ramie (Boehmeria nivea L.) is an important bast fiber crop. To study genetic background of this species, we isolated and characterized microsatellite markers of ramie. A genomic library containing inserts of rapid amplification of polymorphic DNA (RAPD) fragments was constructed, and screened by PCR amplification using anchored simple sequence repeats as primers. A total of 26 clones were identified as positives, and 13 microsatellite loci were found after sequencing. The polymorphism of these 13 microsatellite loci was examined and the utility of simple sequence repeats (SSR) and inter-SSR (ISSR) marker systems for genetic characterization compared using 19 selected ramie cultivars. Both approaches successfully discriminated the 19 cultivars which differed in the amount of polymorphism detected. The level of polymorphism detected by SSR was 95.0%, higher than that by ISSR (72.3%), but the average polymorphism information content (PIC) of ISSR (0.651) was higher than that of SSR (0.441). The higher PIC value of ISSR suggests that ISSR is more efficient for fingerprinting ramie cultivars than SSR markers. However, because the SSR loci are codominant, they are more suitable for determining the homozygosity levels of ramie, constructing linkage map, quantitative trait loci study of complex traits and marker-assisted selection.

关键词： ramie (Boehmeria nivea L.Gaud) simple sequence repeats inter-simple sequence repeats

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Several Theoretical Issues on agricultural Sci-tech Service and Management

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Asian agricultural Research 2012年第5期4卷 67-70页

作者： DU Yan-ping 1 ,WANG Kui-wu 2 ,ZENG Zhao-hui 2 1. college of Humanities and Social Science,hunan agricultural university,changsha 410128,china 2. Science and Technology Department, hunan agricultural university,changsha 410128,china

In view of particularity and complexity of agricultural sci-tech service and management,this paper presents definition of basic concept of agricultural sci-tech service and management,sums up its basic characteristics,and discusses and analyzes its structural elements and functions. The agricultural sci-tech service and management plays an important role in speeding up rural construction and narrowing the gap between urban and rural areas. It ensures benign cycle of scientific and technological achievements and regional environment,and promotes development of agricultural industrialization.

关键词： agricultural sci-tech service agricultural sci-tec

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Analysis of the Genetic Relationships in Chinese Ziziphus with SRAP Markers

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agricultural Sciences in china 2010年第9期9卷 1278-1284页

作者： LI Li, PENG Jian-ying and BAI Rui-xia college of horticulture, agricultural university of Hebei, Baoding 071001, P.R.china [a] college of horticulture Agricultural University of Hebei Baoding 071001 P.R. China

The phylogenic and genetic relationships in germplasm resources of Chinese Ziziphus were studied at molecular levels, for providing new molecular evidences of classification, protection, and utilization of germplasm resources of Ziziphus. The sequence-related amplified polymorphism （SRAP） was assessed to analyse the genetic relationships among 14 species of Ziziphus, 11 cultivars of Z. jujuba Mill., and one outgroup. A total of 580 DNA bands were amplified by 19 selective primers, 570 of which （98.28%） were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 30. The genetic similarities of 26 sample materials were between 0.22 and 0.99. UPGMA method cluster analysis showed that 26 sample materials were classified into six cluster groups with the genetic similarity of 0.28. The results showed that SRAP technique is efficient in studying genetic relationships among Ziziphus, Z. jujuba Mill. and Z. acidojujuba C. Y. Cheng et M. J. Liu which should be treated as one species, and further infraspecific classification of Z. jujuba Mill. should be classified into two subspecies; the scientific names of new taxa, Chinese jujube, and wild jujube were Ziziphus jujuba Mill. subsp, jujuba and Ziziphus jujuba Mill. subsp, spinosa （Bunge） J. Y. Peng, X. Y. Li et L. Li, respectively. Z. xiangchengensis Y. *** et P. K. Chou, Z. montana W.W. Smith and Z. mairei Dode might be originated from same ancestry. Z xiangchengensis Y. L. Chen et P. K. Chou and Z. montana W.W. Smith should be treated as one species. The infrasubspecific taxon of Z. jujuba Mill. was not suitable to set up varieties.

关键词： Ziziphus Mill. genetic relationships SRAP cluster analysis

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