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食管癌组织KAI1/CD_(82)的表达与其生物学行为的关系

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实用临床医药杂志 2004年第3期8卷 52-54页

作者：季润元王成海沈阳李国利冯振卿南京医科大学病理学系江苏南京210029 扬州大学医学院病理教研室江苏扬州225001 江苏省淮安市第二人民医院病理科江苏淮安223302

目的　探讨食管癌组织和正常食管组织中KAI1/CD82 表达的临床意义及其与生物学行为的关系。方法　应用免疫组化SABC法 ,检测 5 2例食管癌及 3 3例食管正常组织中KAI1/CD82 的表达 ,并进行比较分析。结果　食管正常组织、无淋巴结转移食... 详细信息

目的　探讨食管癌组织和正常食管组织中KAI1/CD82 表达的临床意义及其与生物学行为的关系。方法　应用免疫组化SABC法 ,检测 5 2例食管癌及 3 3例食管正常组织中KAI1/CD82 的表达 ,并进行比较分析。结果　食管正常组织、无淋巴结转移食管癌组织和有淋巴结转移食管癌组织KAI1/CD82 的表达率分别为 48 5 % ( 16/3 3 )、 73 7% ( 2 8/3 8)、 2 8 6% ( 4 /14 ) ,食管正常组织、无淋巴结转移食管癌组织、有淋巴结转移食管癌组织KAI1/CD82 的表达有显著差异 (P <0 0 5 )。结论　KAI1/CD82 在食管癌早期高水平表达对肿瘤的恶性进程起抑制作用 ,一旦无法保持KAI1/CD82 的高水平表达则促使肿瘤进入发展期 ,进而发生转移。

关键词： KAI1/CD82 食管癌生物学行为免疫组化

The Relationships between cyclin D1 Expression and Prognosis of Non-small Cell Lung Cancer

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中国肺癌杂志 2010年第8期13卷 803-808页

作者： Jiping ZHU1,Like YU1,Ping ZHAN2,Yong SONG2,Qin WANG3 1Department of Respiratory Medicine,Nanjing Chest Hospital,Nanjing 210029,China 2Department of Respiratory Medicine,Jinling Hospital,Nanjing University School of Medicine,Nanjing 210001,China 3Department of Respiratory Medicine,81 Hospital of PLA,Nanjing 210002,China Department of Respiratory Medicine Nanjing Chest Hospital Nanjing University School of Medicine Nanjing 210029 China.

Background and objective cyclin D1 is a member of the cyclin family,and it has been proven that it plaied an important role in tumorigenesis,invasion and *** performed a retrospective study on the cyclin D1 expression in non-small cell lung cancer (NSCLC) according to the clinical *** One hundred fifteen postsurgical NSCLC patients were *** was used to evaluate the cyclin D1 *** Overall survival was significantly lower in patients with cyclin D1-high expression of tumors than those with cyclin D1 low expression of tumors (χ2=5.132,P=0.023).In early stage patients (stage I,II),the overall survival was significantly lower in patients with cyclin D1-high expression of tumors than those with cyclin D1-low expression of tumors (χ2=6.863,P=0.009).cyclin D1 status (hazard ratio=0.630;P=0.035),differentiation (hazard ratio=0.399;P<0.001),and pTNM (hazard ratio=1.576;P<0.001) to be independent prognostic factors for NSCLC ***,the cyclin D1 status (hazard ratio=0.188;P=0.008) was a significant prognostic factor for patients with stage I *** cyclin D1 expression is an independent prognosis factor for postoperative patient in stage I,II NSCLCs.

关键词：肺癌治疗临床化疗

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大学生鼻变态反应患病率的初步调查

中国中西医结合耳鼻咽喉科杂志 1997年第3期5卷 107-109页

作者：程雷三好彰殷敏陈智斌时海波乔宗海三边武幸由嵘田口喜雄徐其昌殷明德南京医科大学第一附属医院耳鼻咽喉科 210029 日本东京都立荏原病院耳鼻咽喉科日本东北大学医学系研究科异文化健康学教研室

采用问诊表、鼻腔检查和变应原皮肤试验，于1995年和1996年对南京医科大学863名医学生进行鼻变态反应患病率调查。结果显示：变应原皮论总阳性率43．8％，鼻变态反应患病率1．2％。作者结合文献进行了分析和讨论，认为有必要对变应原皮... 详细信息

采用问诊表、鼻腔检查和变应原皮肤试验，于1995年和1996年对南京医科大学863名医学生进行鼻变态反应患病率调查。结果显示：变应原皮论总阳性率43．8％，鼻变态反应患病率1．2％。作者结合文献进行了分析和讨论，认为有必要对变应原皮试阳性但未发生鼻变态反应者进行流行病学追踪观察。

关键词：鼻变态反应患病率大学生调查

基于神经网络的大跨空间结构脉动风荷载的随机模拟

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特种结构 2006年第2期23卷 1-3页

作者：丁幼亮李爱群杜东升张志强任家骥仓慧勤东南大学土木工程学院混凝土及预应力混凝土结构教育部重点实验室江苏省建筑设计研究院江苏省建筑设计研究院南京 210096 南京 210029

研究了大跨空间结构脉动风荷载的随机模拟技术,基于神经网络进行了风速时程的随机模拟。将该数值模拟技术应用于南京奥林匹克体育中心体育场的随机风荷载模拟,计算结果表明了该方法的有效性。

关键词：大跨空间结构风荷载神经网络随机模拟

Ultra-high-performance liquid chromatography-quadrupole/time of flight mass spectrometry based chemical profiling approach to rapidly reveal chemical transformation of sulfur-fumigated medicinal herbs,a case study on white ginseng

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Ultra-high-performance liquid chromatography-quadrupole/time...

Two different molecular mechanisms underlying progesterone neuroprotection against ischemic brain damage

中药与天然药高峰论坛暨第十二届全国中药和天然药物学术研讨会

作者： Song-Lin Li~(a,*),Hong Shen~a,Ling-Ying Zhu~a,Jun Xu~a,Xiao-Bin Jia~a,Hong-Mei Zhang~b,Ge Lin~c, Hao Cai~d,Bao-Chang Cai~d,Shi-Lin Chen~e,Hong-Xi Xu~(b,*) a Department of Metabolomics and Key Laboratory of New Drug Delivery System of Chinese Materia Medica,Jiangsu Province Academy of Chinese Medicine,Nanjing 210028,PR China b School of Chinese Medicines,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,PR China c School of Biomedical Sciences,The Chinese University of Hong Kong,Hong Kong SAR,PR China d Nanjing Haichang Chinese Medicine Co.Lit and Nanjing University of Chinese Medicine, Nanjing 210029,PR China e The Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,PR China

Sulfur-fumigation may induce chemical transformation of medicinal *** of rapid method to reveal potential sulfur-fumigation induced chemical transformation of herbs is a very important issue for efficacy and safety of herb *** present study,a new strategy was proposed to rapidly reveal chemical transformation of sulfur-fumigated herbs by ultra-high-performance liquid chromatography-quadrupole/time of flight mass spectrometry (UHPLC-QTOF-MS/MS)based chemical profiling *** non-fumigated herb was water-wetted and further treated with burning sulfur to get sulfur-fumigated *** the chemical fingerprints of both non-fumigated and sulfur-fumigated samples were compared by UHPLC-QTOF-MS/MS *** identities of all detected peaks,in particular those newly-generated in sulfur-fumigated samples were confirmed by comparing the mass spectra and retention times of peaks with that of reference compounds,and/or tentatively assigned by matching empirical molecular formula with that of published compounds,and/or elucidating quasi-molecular ions and fragment ions referring to available literature *** identification could be rationalized through deducing possible reactions involved in the generation of these newly detected *** proposed strategy was extensively investigated in the case of white *** 82 components were detected in non-fumigated and sulfur-fumigated white ginseng samples,among them 35 sulfur-containing compounds detected only in sulfur-fumigated white ginseng and its decoction were assigned to be sulfate or sulfite derivatives of original ginsenosides,and were deduced to be generated via reactions of esterification,addition,hydrolysis and dehydration during sulfur-fumigation and decocting of white *** established approach was applied to discriminate sulfur-fumigated white ginseng among commercial samples from America,Canada,and Hong Kong SAR,Macau SAR and Mainland of China,which indicated

关键词： UHPLC-QTOF-MS/MS Ginseng Ginsenoside Chemical transformation Sulfur-fumigation

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南京医科大学学报(自然科学版) 2008年第10期 1355-1355页

作者： Cai, W. Y. Zhu, Y. Furuya, K. Li, Z. Sokabe, M. Chen, L. Nanjing Med Univ, Lab Reprod Med, Dept Physiol, Nanjing 210029, Jiangsu, Peoples R China. Nagoya Univ, Grad Sch Med, Dept Physiol, Nagoya, Aichi 4668550, Japan. JST, ICORP SORST Cell Mechanosensing, Nagoya, Aichi 4668550, Japan. Natl Inst Physiol Sci, Dept Mol Physiol, Okazaki, Aichi 4448585, Japan.

Herein, we show that a single injection of P4(4 mg/kg) at 1 h or 48 h, but not 96 h, before middle cerebral artery occlusion (MCAO) produces significant protective effects against the ischemia-induced neuronal death and the deficits in spatial cognition and UP induction. The present study focused on the molecular mechanisms underlying the neuroprotection exerted by P4 administration at I h and 48 h pre-MCAO, termed acute and delayed P4-neuroprotection, respectively. Pharmacology suggested that P4-receptor(P4R) cascading to a Src-ERK1/2 signaling mediated the delayed P4-neuroprotection. To support this, it was observed by anti-phosph-ERK1/2 immunoblots that a single injection of P4 triggered a P4R-mediated persistent increase in ERK1/2 phosphorylation and their nuclear translocation for 48 h. In contrast, the acute P4-neuroprotection did not depend on the P4R-mediated Src-ERK1/2 signaling. Instead, the acute P4-administration attenuated the NMDA-induced rise in the intracellular calcium concentration([Ca2+] (i) ) that may be a primary cause for MCAO-induced neuronal injury. This effect seemed to be exerted by an antagonism of sigma(1) receptor since the sigma(1) receptor antagonist NE100 perfectly mimicked the acute P4-neuroprotection and also attenuated the NM- DA-incluced [Ca2+] (i), increase. These findings suggest that the P4 neuroprotection involves two independent processes depending on the timing of P4 administration before MCAO: an acute protection by antagonizing sigma (1) receptor to inhibit NMDAr-Ca2+ influx and a delayed one by an activation of P4R-mediated SrcERK signaling pathway. (C) 2008 Elsevier Ltd. All rights reserved.

关键词： progesterone(P4) middle cerebral artery occlusion(MCAO) extracellular receptor kinase(ERK) NMDA receptor (NMDAr) sigma1 (sigma (1) ) receptor cerebral-artery occlusion long-term potentiation methyl-d-aspartate preconditioning-induced activation cerebellar purkinje-cells hippocampal ca1 region rat hippocampus protein-kinase nmda receptor tyrosine phosphorylation

液相色谱-串联质谱法测定人血浆中倍他米松的浓度

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中国新药与临床杂志 2011年第3期30卷 220-225页

作者：谭洁贺春晖刘江慧余潜胡云芳程俊霖于翠霞周洁樊宏伟朱余兵邹建军南京医科大学附属南京第一医院国家药物临床试验机构江苏南京210006 南京医科大学药学院江苏南京210029

目的建立快速、灵敏的液相色谱-串联质谱法测定人血浆中倍他米松(BOH)浓度的方法。方法采用Agilent Zorbax C_(18)(2.1mm×150mm,5μm)色谱柱,柱温25℃,流动相为甲醇-水(85:15,V/V),流速为0.2mL·min^(-1),电喷雾(ESI)离子源,多反应离... 详细信息

目的建立快速、灵敏的液相色谱-串联质谱法测定人血浆中倍他米松(BOH)浓度的方法。方法采用Agilent Zorbax C_(18)(2.1mm×150mm,5μm)色谱柱,柱温25℃,流动相为甲醇-水(85:15,V/V),流速为0.2mL·min^(-1),电喷雾(ESI)离子源,多反应离子监测(MRM)的扫描方式。BOH采用负离子检测,二丙酸倍氯米松(内标,IS)采用正离子检测,BOH和IS的离子通道分别选择为m/z 361.0→m/z307.2和m/z 543.1→m/z 433.2。结果 BOH在0.1～30μg·L^(-1)浓度范围内有良好的线性关系(r^2=0.999 1),定量下限(LOQ)为0.1μg·L^(-1),日内、日间精密度(RSD)均小于15%,提取回收率均在65%左右,相对回收率均在85%～115%范围内。结论该方法简便、准确、灵敏,可用于人血浆中BOH的浓度检测、人体药动学和生物等效性研究。

关键词：倍他米松色谱法,高压液相光谱法,质量,电喷雾电离

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复苏困难的重症瓣膜病人心肌保护方法

中国体外循环杂志 2003年第4期1卷 237-238页

作者：梁永年陈亦江陈广明邵永丰骆金华吴延虎朱小鸾南京医科大学第一附属医院心胸外科江苏南京210029

目的总结9例复苏困难的重症瓣膜病患者成功复苏经验,尝试心肌保护新方法。方法对经多次除颤不能复苏的重症瓣膜病患者,采用再次阻断升主动脉,行4:1半钾温冷血停搏液诱停加纯温血持续灌注,待心脏复苏后再开放升主动脉的方法。结果9例复... 详细信息

目的总结9例复苏困难的重症瓣膜病患者成功复苏经验,尝试心肌保护新方法。方法对经多次除颤不能复苏的重症瓣膜病患者,采用再次阻断升主动脉,行4:1半钾温冷血停搏液诱停加纯温血持续灌注,待心脏复苏后再开放升主动脉的方法。结果9例复苏困难的重症瓣膜病患者均自动复苏。结论再次阻断升主动脉,行4:1半钾温冷血停搏液诱停加纯温血(不含停搏液)持续灌注,待心脏复苏后再开放升主动脉的方法为复苏困难的心脏直视手术患者成功复苏提供了新方法。

关键词：重症瓣膜病心脏复苏困难心肌保护升主动脉温血灌注体外循环

氨曲南治疗60例老年下呼吸道细菌感染的多中心随机对照研究

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中国新药与临床杂志 2004年第1期23卷 13-17页

作者：唐英春张扣兴张月莉殷凯生戴令娟庞仲华张德平戴山林中山大学第三附属医院呼吸内科广东广州510630 华东医院呼吸内科上海200040 南京大学附属一院呼吸内科江苏南京210029 南京大学附属鼓楼医院呼吸内科江苏南京210008

目的 :评价氨曲南治疗老年下呼吸道细菌感染的疗效和安全性。方法 :老年人下呼吸道细菌感染 12 1例 ,随机分氨曲南组 6 0例 ,用氨曲南2 .0 g ,加入氯化钠注射液 10 0mL中 ,iv ,gtt ,q 12h ,疗程 7～ 14d ;头孢他啶组 6 1例 ,用头孢他啶2... 详细信息

目的 :评价氨曲南治疗老年下呼吸道细菌感染的疗效和安全性。方法 :老年人下呼吸道细菌感染 12 1例 ,随机分氨曲南组 6 0例 ,用氨曲南2 .0 g ,加入氯化钠注射液 10 0mL中 ,iv ,gtt ,q 12h ,疗程 7～ 14d ;头孢他啶组 6 1例 ,用头孢他啶2 .0 g,加入氯化钠注射液 10 0mL中 ,iv ,gtt ,q 12h ,疗程 7～ 14d。结果 :氨曲南组和头孢他啶组有效率分别为 83%和 80 % ,痊愈率分别为 5 8%和5 4% ,差异无显著意义 (P >0 .0 5 )。 2组细菌清除率分别为 85 %和 82 % ,细菌阴转率分别为 82 %和82 % ,差异无显著意义 (P >0 .0 5 ) ;2组不良反应发生率分别为 2 %和 3% ,实验室检查异常发生率分别为 3%和 3% ,均系轻度呈一过性 ,差异无显著意义 (P >0 .0 5 )。结论 :氨曲南治疗老年下呼吸道细菌感染的安全。

关键词：细菌感染老年人多中心研究随机对照试验氯曲南头孢他啶