检索结果-南通市图书馆

Cloning, Expression, Purification and Crystallization of NHR3 Domain from Acute Myelogenous Leukemia-related Protein AML1-ETO

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第8期36卷 566-570页

作者： Hai-Tao YANG , Dong-Hui WU1 , Xiao-Yu XUE, Wen-Xue LIANG1, Xiao-Yu MIAO,Hai PANG , and Sai-Juan CHEN1Laboratory of Structural Biology, Department of Biological Sciences and Biotechnology & Protein Sciences Laboratory of MOE, Tsinghua University, Beijing 100084, China Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics,1 Shanghai Second Medical University, Shanghai 200023, China Laboratory of Structural Biology Department of Biological Sciences and Biotechnology & Protein Sciences Laboratory of MOE Tsinghua UniversityBeijing 100084 China Shanghai Institute of Hematology State Key Laboratory of Medical Genomics Shanghai Second Medical UniversityShanghai 200023 China

The t(8;21) translocation is one of the most frequent chromosome abnormalities in acute myeloid leukemia. This translocation creates a fusion between the acute myelogenous leukemia 1 (AML1, a transcription factor) gene on chromosome 21 and the eight-twenty-one (ETO, a zinc finger nuclear protein) gene on chromosome 8, leading to the repression of certain AML1 target genes. We cloned NHR3 domain coding fragment into vector pGEX-6p-1 using PCR and obtained recombinant plasmid pGEX-6p-1-NHR3, which can be induced to stably overexpress fusion protein in E. coli. Through the purification on GST affinity chromatography column and PreScission protease cleavage, a large amount of NHR3 protein with high purity was obtained. In order to avoid possible interference of some strong negative charged molecules, NHR3 protein was further purified by Mono Q anion exchange chromatography. The NHR3 crystals were grown with hanging drop/vapor diffusion method and the first crystals appeared after four weeks at 18 °C in 0.2 M Tris-sodium citrate dihydrate, 0.1 M sodium cacodylate, pH 6.5, and 30% iso-propanol (V/V). ESI mass spectrum showed that the molecular weight of this domain was in agreement with its primary structure sequence prediction, and circular dichroism spectral data (190–250 nm) showed that NHR3 had a well-defined secondary structure of 25.9% α-helix, 23.2% random coil and 50.9% turn, which was consistent with GOV4 software prediction.

关键词： AML1-ETO NHR3 crystallization ESI mass spectrum circular dichroism spectrum glutathione S-transferase (GST)

Effect of salvianolic acid B on Smad3 expression in hepatic stellate cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2004年第1期3卷 102-105页

作者： Jun-Fang Zhao, Cheng-Hai Liu, Yi-Yang Hu, Lie-Ming Xu, Ping Liu and Cheng Liu Shanghai, China Institute of Liver Diseases, Shanghai University of Tra- ditional Chinese Medicine, Shanghai 200023 , China Institute of Liver Diseases Shanghai University of Traditional Chinese Medicine Shanghai 200023 China.

BACKGROUND: Salvianolic acid B (SA-B), one of water soluble compounds derived from Radix salviae miltiorrhizae, had good action against liver fibrosis of patients with chro- nic hepatitis. Hepatic stellate cells (HSCs) is the cellular re- source for liver fibrogenesis, while transforming growth factor-β1 (TGF-β1) is most potent fibrogenic factor. In this study we investigated the mechanism of SA-B action against liver fibrosis relating to the interference with TGF- β1 signaling at HSC. METHODS: Hepatic stellate cells (HSCs) were isolated, cultured, and incubated with SA-B. The TGF-β1 content in the supernatant of subcultured HSCs was assayed with ELISA. Type I collagen and Smad3 protein in TGF-β1-sti- mulated primarily cultured HSCs for 4 days were detected by Western blot. RESULTS: TGF-β1 secreted in activated HSCs was more than in primary HSCs, and SA-B significantly decreased TGF-β1 secretion in activated HSCs. TGF-β1 increased the expression of type I collagen and Smad3 protein in d4 pri- mary HSCs, while SA-B inhibited their expression. CONCLUSIONS: SA-B inhibits TGF-β1 secretion in activa- ted HSCs and counteracts the expression of TGF-β1 stimu- lated type I collagen and Smad3. These actions are associat- ed with the effect of SA-B on liver fibrosis.

关键词： salvianolic acid B liver fibrosis transforming growth factor-β Smads

The Design of Intelligent Corporate E-commerce System

在线全文

学校读者我要写书评

暂无评论

厦门大学学报（自然科学版） 2002年第S1期41卷 265-266页

作者： ZHANG Qing-bian 1, ZHANG Fu-hai 2, ZHANG Li-fang 1 (1. Dept. of Electronic Engineering, Xiamen University, Xiamen 361005 , China 2. Corporation of Kingside Network Ltd, Shanghai 200023, China)

The paper shows how to design and implement an inte ll igent e-commerce system for medium or small enterprises. The corporate intranet is connected with Internet, which constitutes the hardware of the system. The s ystem adopts modern voice-identification technology for user authentication to enable secure access to key databases and thus enhance the security of system da tabases. The software system consists of four modules: 1. Corporate website and advertisement information system, intending for updatin g online advertisement, collecting and analyzing market research information. 2. Employee technology training and assessment system, including a variety of co urses containing technology, technique and management and so on. Login the training system, employees are able to study courses of technology or technique or others. The training system adopts modern multimedia E-Learning te chnology and in fact it is an innovative education method, which includes self- study, tutorship and lecture. It is able to help employees enjoy their learning. Employees can take simulative exams, and managers can test employees onlin e periodically. 3. Corporate decision-making management system, calculating and analyzing the o peration, predicting future development, facilitating communication between mana gers and employees. It provides valuable foundation for decisions, which enables managers to make strategic decisions and scientific management. 4. E-commerce online transaction system, which facilitates material purchase an d product sale. By using intelligent e-commerce system, corporations will enhance the employee’ s skills and management level, reduce production cost, promote product sale , and thus strengthen corporation’s market competitiveness effectively.

关键词： e-commerce corporate decision management techn ology training and assessment

The Seasonal Variation Characters of Natural Vegetation and its Application in Green Space

在线全文

学校读者我要写书评

暂无评论

The Seasonal Variation Characters of Natural Vegetation and ...

第8届中日韩国际风景园林学术研讨会

作者： TIAN Qi YANG Wen-yue Shanghai Institute of Landscape and Gardening. Shanghai. 200232 Shanghai Landscaping Administration Bureau Shanghai. 200023

＜正＞The characters of north-subtropical climate decide that the typical vegetation in Shanghai is evergreen and deciduous broad-leaved forest, which has obvious seasonal variation. The climate-based law for residents to go into the green space shows that, the people prefer to go into the gardens or parks for the beautiful flowers on the trees and shrubs in shining spring days, for colorful scenery of seasonal changed leaves and fruits in golden autumn, for nice and cool shade of trees in summer, and for warm sunny in winter. Following the natural characters and man’s needs above, the landscape in Shanghai should be built with plentiful flowers in spring, more nice plant shade in summer, colorful scenery in autumn, sunny ground without shade in winter. In order to improve the seasonal variation ol green space, some checklists of seasonal changed plants are provided, and some technical advice for garden plan and design is also given.

关键词： Landscape plants Vegetation Study: Green Space Seasonal Variation Shanghai

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

英国和德国危险场所用电气设备的接地实践

石油化工自动化 1998年第2期 14-17+26+3页

作者： Provided by MTL Instrument Co.Shanghai Office,Shanghai,200023 英国ＭＴＬ仪器仪表集团公司上海办事处

在欧洲共同体范围内，由欧洲电工委员会（ＣＥＮＥＬＥＣ）制订的《防爆电气设备技术标准》已明显被接受。现行的关于爆炸性环境的指令也已由批准的认证机构之一认可的电气设备具有较高程度的可接受性。最新的爆炸性环境用电气设备的指... 详细信息

在欧洲共同体范围内，由欧洲电工委员会（ＣＥＮＥＬＥＣ）制订的《防爆电气设备技术标准》已明显被接受。现行的关于爆炸性环境的指令也已由批准的认证机构之一认可的电气设备具有较高程度的可接受性。最新的爆炸性环境用电气设备的指令将产生更进一步的状态变化。阐述了尽管现行的指令仅仅涉及设备，但是设备设计和安装实践之间不可避免地存在有相互作用关系。特别是电气设备必须具有接地防护措施，而且如果消除故障和过载的方法是已知的，那么唯一可进行的是设备的安全性评定。介绍了英国的接地和接线技术在某些方面与德国的接地和接线技术存在有明显的不同（抵触）。并通过对两种技术方法的讨论，试图说明它们之间没有特别意义的区别，任何一种技术都将产生一个可接受的安全结果。

关键词：危险场所电气设备本安回路接地

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

基于RS485通信的染色机计算机控制系统

丝绸 2004年第7期41卷 26-29页

作者：张永明老正和染厂上海200023

论述了可编程控制器(PLC)网络在工业控制中的应用。以个人计算机(PC)作上位机,小型可编程控制器(PLC)为控制核心,染色单片机作自动温控时的控制模块,智能数字显示调节仪作模拟信号采集模块及手动温控时的上限控制,组成基于RS485通信的小... 详细信息

论述了可编程控制器(PLC)网络在工业控制中的应用。以个人计算机(PC)作上位机,小型可编程控制器(PLC)为控制核心,染色单片机作自动温控时的控制模块,智能数字显示调节仪作模拟信号采集模块及手动温控时的上限控制,组成基于RS485通信的小型PLC网络控制系统,应用于对拥有多台染色机的自动化管理监控。给出了具体的系统方案、网络拓扑结构及通信协议等硬件配置和通信软件的设计。本方案以集中监管、分散控制、简单可靠、高性价比为设计理念,集个人计算机、PLC、单片机、VB语言通信技术应用为一体,具有很好的可扩展性和实用性。

关键词：可编程控制器染色机计算机控制系统温度控制模块模拟信号采集工业控制织物